BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	673,104	T→C	K301K (AAA→AAG)	leuS ←	leucine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	673,104	0	T	C	99.6%	710.4 / ‑5.0	224	K301K (AAA→AAG)	leuS	leucine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (124/98); total (126/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

Only 100 of 244 total aligned reads displayed.

GCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTCCGC  >  NC_000913/673014‑673193

gCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCctt                                                                                         <  2:196779/93‑1 (MQ=255)

gCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCctt                                                                                         <  1:5331415/93‑1 (MQ=255)

gCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCctt                                                                                         >  1:2082825/1‑93 (MQ=255)

gCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCctt                                                                                         <  1:5785585/93‑1 (MQ=255)

 ccGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCcttt                                                                                        <  2:3458322/93‑1 (MQ=255)

  cGTTCTCCATAAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCctttt                                                                                       <  1:4053945/93‑1 (MQ=255)

   gTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCcttttt                                                                                      <  1:5515331/93‑1 (MQ=255)

    tACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTtc                                                                                     <  2:6439099/93‑1 (MQ=255)

     aCTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTtct                                                                                    >  1:5768101/1‑93 (MQ=255)

       tCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTcc                                                                                  >  2:4750339/1‑93 (MQ=255)

       tCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTcc                                                                                  <  1:5911654/93‑1 (MQ=255)

       tCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTcc                                                                                  <  1:2021999/93‑1 (MQ=255)

        ccATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCa                                                                                 >  1:2646042/1‑93 (MQ=255)

        ccATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCa                                                                                 >  2:76223/1‑93 (MQ=255)

         cATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAt                                                                                <  2:835738/93‑1 (MQ=255)

          aTCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtc                                                                               <  2:692789/93‑1 (MQ=255)

          aTCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtc                                                                               >  1:3069313/1‑93 (MQ=255)

          aTCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtc                                                                               >  1:1785205/1‑93 (MQ=255)

          aTCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtc                                                                               >  2:2657188/1‑93 (MQ=255)

           tCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtcg                                                                              <  2:5578113/93‑1 (MQ=255)

             aaTACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtcgtc                                                                            >  2:6344689/1‑93 (MQ=255)

              aTACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtcgtcg                                                                           <  1:6498414/93‑1 (MQ=255)

              aTACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtcgtcg                                                                           >  1:6257054/1‑93 (MQ=255)

              aTACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCAtcgtcg                                                                           >  2:4432333/1‑93 (MQ=255)

                aCGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGcc                                                                         >  2:6586824/1‑93 (MQ=255)

                 cGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCa                                                                        >  1:1010524/1‑93 (MQ=255)

                 cGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCa                                                                        >  1:4929899/1‑93 (MQ=255)

                  gAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCAt                                                                       >  2:3092112/1‑93 (MQ=255)

                  gAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCAt                                                                       >  2:4486883/1‑93 (MQ=255)

                  gAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCAt                                                                       <  2:3601197/93‑1 (MQ=255)

                  gAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCAt                                                                       <  1:2580682/93‑1 (MQ=255)

                  gAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATAGTCGCCAt                                                                       >  1:5917432/1‑93 (MQ=255)

                    aGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCAttt                                                                     >  2:4185114/1‑93 (MQ=255)

                     gTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTc                                                                    <  2:3732842/93‑1 (MQ=255)

                     gTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTc                                                                    >  1:1388526/1‑93 (MQ=255)

                       ttGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAg                                                                  <  2:5037141/93‑1 (MQ=255)

                        tgctgcCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGc                                                                 <  1:3612024/93‑1 (MQ=255)

                         gctgcCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCt                                                                >  1:4118773/1‑93 (MQ=255)

                         gctgcCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCt                                                                <  1:668724/93‑1 (MQ=255)

                              ccAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGc                                                           >  2:2280747/1‑93 (MQ=255)

                              ccAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGc                                                           <  2:4797826/93‑1 (MQ=255)

                               cAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCa                                                          >  1:3503147/1‑93 (MQ=255)

                                aaaCGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCac                                                         >  2:914486/1‑92 (MQ=255)

                                aaaCGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCaa                                                         >  1:2473899/1‑93 (MQ=255)

                                 aaCGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAAc                                                        <  1:3658622/93‑1 (MQ=255)

                                  aCGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACt                                                       <  1:3018680/93‑1 (MQ=255)

                                   cGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACtt                                                      <  1:4690498/93‑1 (MQ=255)

                                    gggAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACttt                                                     <  1:4945503/93‑1 (MQ=255)

                                    gggAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACttt                                                     >  1:1851205/1‑93 (MQ=255)

                                     gaaaTTTCTTCGCCCGTGAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTg                                                    <  1:6172941/91‑1 (MQ=255)

                                        aTTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGgtg                                                 >  1:5122288/1‑93 (MQ=255)

                                        aTTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCCCCATCGTCGCCATTTCAGCTTCGGCAACTTTGgtg                                                 <  2:6189714/93‑1 (MQ=255)

                                          ttcttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGtt                                               >  2:3828873/1‑93 (MQ=255)

                                          ttcttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGtt                                               <  2:2926731/93‑1 (MQ=255)

                                          ttcttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGtt                                               >  1:2290188/1‑93 (MQ=255)

                                          tccttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGtt                                               <  2:1237886/91‑1 (MQ=255)

                                            cttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCATCTTCGGCAACTTTGGTGTTAc                                             <  1:4240350/93‑1 (MQ=255)

                                            cttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTAc                                             <  1:4511379/93‑1 (MQ=255)

                                            cttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTAc                                             <  2:1821439/93‑1 (MQ=255)

                                            cttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTAc                                             <  1:2756915/93‑1 (MQ=255)

                                             ttcGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACg                                            >  2:6118132/1‑93 (MQ=255)

                                                gCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCa                                         <  2:5363111/93‑1 (MQ=255)

                                                 cccGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCAt                                        <  1:1437449/93‑1 (MQ=255)

                                                  ccGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCAtt                                       >  1:3731894/1‑93 (MQ=255)

                                                      tAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTc                                   <  1:2890594/93‑1 (MQ=255)

                                                       aaTGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCa                                  >  1:4584812/1‑93 (MQ=255)

                                                        aTGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCaa                                 <  1:5233640/93‑1 (MQ=255)

                                                        aTGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCaa                                 >  1:3832640/1‑93 (MQ=255)

                                                         tGGGTGAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAAt                                >  1:421335/1‑93 (MQ=255)

                                                              gAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAgg                           <  1:754229/93‑1 (MQ=255)

                                                              gAACCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAgg                           >  2:1104171/1‑93 (MQ=255)

                                                               aaCCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGgc                          <  2:2124272/93‑1 (MQ=255)

                                                                aCCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGgcc                         <  2:2510509/93‑1 (MQ=255)

                                                                aCCGCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGgcc                         >  2:5277512/1‑93 (MQ=255)

                                                                   gCTTTAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGgccgcc                      >  1:916232/1‑93 (MQ=255)

                                                                     tttAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAg                    >  1:3338446/1‑93 (MQ=255)

                                                                     tttAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAg                    <  2:6028187/93‑1 (MQ=255)

                                                                     tttAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAg                    >  1:4573955/1‑93 (MQ=255)

                                                                      tgaaaGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGt                   <  1:2970006/91‑1 (MQ=255)

                                                                       tAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGtt                  >  2:158078/1‑93 (MQ=255)

                                                                       tAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGtt                  <  1:5451833/93‑1 (MQ=255)

                                                                       tAAAGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGtt                  >  1:1294428/1‑93 (MQ=255)

                                                                        aaaGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTc                 >  1:392497/1‑93 (MQ=255)

                                                                         aaGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGATTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCa                >  2:1686811/1‑93 (MQ=255)

                                                                          aGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAg               >  2:3926418/1‑93 (MQ=255)

                                                                          aGCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAg               >  1:4969683/1‑93 (MQ=255)

                                                                           gCCAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAgg              >  1:6287902/1‑93 (MQ=255)

                                                                            ccAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGa             >  2:2250204/1‑93 (MQ=255)

                                                                             cAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGat            <  1:3152916/93‑1 (MQ=255)

                                                                             cAGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGat            >  2:5746161/1‑93 (MQ=255)

                                                                              aGTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATCCGTCAATAAAGGCCGCCAGTTCAGGatt           <  1:5992500/93‑1 (MQ=255)

                                                                               gTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGattt          >  1:6325462/1‑92 (MQ=255)

                                                                               gTATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGatta          >  2:5899982/1‑93 (MQ=255)

                                                                                tATCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGattat         <  2:2410950/93‑1 (MQ=255)

                                                                                 aTCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGattatt        <  1:3570205/93‑1 (MQ=255)

                                                                                 aTCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGattatt        >  1:6070775/1‑93 (MQ=255)

                                                                                 aTCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGAATTCGTCAATAAAGGCCGCCAGTTCAGGattatt        >  1:1209350/1‑93 (MQ=255)

                                                                                  tCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTAttt       <  2:4033113/93‑1 (MQ=255)

                                                                                  tCGACGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTAttt       <  2:2777566/93‑1 (MQ=255)

                                                                                      cGCCCTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTccg   <  1:2415847/93‑1 (MQ=255)

GCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCATCGTCGCCATTTCAGCTTCGGCAACTTTGGTGTTACGGCATTCGTCAATAAAGGCCGCCAGTTCAGGATTATTTTCCGC  >  NC_000913/673014‑673193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`