Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 763,150 T→C I305T (ATC→ACC)  sucC → succinyl‑CoA synthetase beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913763,1500TC99.6% 749.2 / ‑4.7 232I305T (ATC→ACC) sucCsuccinyl‑CoA synthetase beta subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (118/112);  total (120/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 250 total aligned reads displayed.

TCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCG  >  NC_000913/763060‑763239
                                                                                          |                                                                                         
tCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCAccc                                                                                         <  2:1298735/93‑1 (MQ=255)
tCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCAccc                                                                                         >  1:5682643/1‑93 (MQ=255)
   ttAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCctct                                                                                      >  1:574970/1‑93 (MQ=255)
    tAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCctctc                                                                                     <  1:4026274/93‑1 (MQ=255)
    tAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCctctc                                                                                     <  1:2819976/93‑1 (MQ=255)
     aaaCTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCCCCctctct                                                                                    <  1:4179083/93‑1 (MQ=255)
     aaaCTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCctctct                                                                                    >  2:5892904/1‑93 (MQ=255)
       aCTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTga                                                                                  >  2:3612286/1‑93 (MQ=255)
           cACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTgacgac                                                                              <  1:1279249/93‑1 (MQ=255)
            aCGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACa                                                                             >  1:3223230/1‑93 (MQ=255)
            aCGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACa                                                                             >  2:5180754/1‑93 (MQ=255)
              ggcggcGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACaaa                                                                           >  2:5148610/1‑93 (MQ=255)
               gcggcgAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAg                                                                          >  2:2364210/1‑93 (MQ=255)
               gcggcgAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAg                                                                          >  1:3418904/1‑93 (MQ=255)
               gcggcgAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGAGTTCAAAATCACCCTCTCTGACGACAAAg                                                                          >  2:1060942/1‑93 (MQ=255)
                 ggcgAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTg                                                                        <  1:2797965/93‑1 (MQ=255)
                  gcgAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGa                                                                       >  2:6722318/1‑93 (MQ=255)
                  gcgAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGa                                                                       <  1:6701775/93‑1 (MQ=255)
                   cgAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGaa                                                                      <  1:2485199/93‑1 (MQ=255)
                   cgAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGaa                                                                      <  1:2024952/93‑1 (MQ=255)
                    gAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCCACCAAAGAACGTGTAACCGAAACGTTCAAAAAAACCCCCTCTTACGACAAAGAGaaa                                                                     >  2:4720960/1‑93 (MQ=255)
                    gAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGaaa                                                                     <  1:2309673/93‑1 (MQ=255)
                    gAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGaaa                                                                     >  1:2233407/1‑93 (MQ=255)
                    gAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGaaa                                                                     >  2:5747125/1‑93 (MQ=255)
                    gAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGaaa                                                                     >  1:555215/1‑93 (MQ=255)
                     aaCCGGCTAACTTCCTTGACGTTGGTGGCGGCGCAACCAAAGAACGGGTAACCCAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAg                                                                    >  1:3293471/1‑93 (MQ=255)
                      aCCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGc                                                                   <  2:1827689/93‑1 (MQ=255)
                      aCCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGc                                                                   >  1:6053212/1‑93 (MQ=255)
                         ggCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGt                                                                <  1:5275659/93‑1 (MQ=255)
                           cTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTc                                                              >  1:487166/1‑93 (MQ=255)
                           cTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTATAACCGAAGCGTTCAAAATCGCCCTCTCTGACGACAAAGTGAAAGCCGTTc                                                              <  2:1581164/93‑1 (MQ=255)
                            tAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCt                                                             <  1:5101883/93‑1 (MQ=255)
                              aCTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTgg                                                           >  1:2544003/1‑93 (MQ=255)
                              aCTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTgg                                                           >  1:4803981/1‑93 (MQ=255)
                               cTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGt                                                          <  1:1794225/93‑1 (MQ=255)
                                ttCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGtt                                                         <  1:3711500/93‑1 (MQ=255)
                                ttCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGtt                                                         <  2:3271074/93‑1 (MQ=255)
                                ttCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGtt                                                         <  2:6526996/93‑1 (MQ=255)
                                  ccTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTaa                                                       >  2:178385/1‑93 (MQ=255)
                                   cTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACCAAGTGAAAGCCGTTCTGGGTAAc                                                      >  2:6381570/1‑93 (MQ=255)
                                   cTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAAc                                                      <  2:5479767/93‑1 (MQ=255)
                                    ttGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATGACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACa                                                     >  1:5203490/1‑93 (MQ=255)
                                    ttGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACa                                                     <  2:6333005/93‑1 (MQ=255)
                                    ttGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACa                                                     >  1:953494/1‑93 (MQ=255)
                                       aCGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCt                                                  <  1:852874/93‑1 (MQ=255)
                                        cGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCtt                                                 <  2:4869969/93‑1 (MQ=255)
                                          ttGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTcg                                               >  1:4071254/1‑93 (MQ=255)
                                           tGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTcgg                                              >  2:4492228/1‑93 (MQ=255)
                                           tGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTcgg                                              <  1:5825630/93‑1 (MQ=255)
                                            ggcggcggcGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTcggc                                             >  2:3257525/1‑93 (MQ=255)
                                                 cggcgCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAt                                        <  1:749772/93‑1 (MQ=255)
                                                 cggcgCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAt                                        >  2:4042958/1‑93 (MQ=255)
                                                  ggcgCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAtc                                       <  1:465998/93‑1 (MQ=255)
                                                   gcgcAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAtcg                                      <  1:5427485/93‑1 (MQ=255)
                                                    cgcAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAtcgt                                     <  2:1396485/93‑1 (MQ=255)
                                                     gcAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAtcgtt                                    >  2:5819321/1‑93 (MQ=255)
                                                      cAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAtcgttc                                   >  2:2275954/1‑93 (MQ=255)
                                                       aaCCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAtcgttcg                                  <  2:5291259/93‑1 (MQ=255)
                                                       aaCCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAtcgttcg                                  <  1:203756/93‑1 (MQ=255)
                                                        aCCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAtcgttcgt                                 >  2:6239400/1‑93 (MQ=255)
                                                        aCCAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTAtcgttcgt                                 >  2:4974248/1‑93 (MQ=255)
                                                         ccAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATcgttcgtt                                <  1:1643380/93‑1 (MQ=255)
                                                          cAAAGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTg                               <  1:291755/93‑1 (MQ=255)
                                                            aaGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCg                             >  2:4297302/1‑93 (MQ=255)
                                                            aaGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCg                             >  1:385727/1‑93 (MQ=255)
                                                            aaGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCg                             >  2:3427069/1‑93 (MQ=255)
                                                            aaGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCg                             <  2:1349818/93‑1 (MQ=255)
                                                             aGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGa                            >  1:1584799/1‑93 (MQ=255)
                                                             aGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGa                            <  2:5758763/93‑1 (MQ=255)
                                                             aGAACGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGa                            >  1:6360045/1‑93 (MQ=255)
                                                               aaCGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAGCATCTTCGGCGGTATCGTTCGTTGCGAcc                          <  1:1515778/93‑1 (MQ=255)
                                                               aaCGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGAcc                          >  2:2437790/1‑93 (MQ=255)
                                                                aCGTGTAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCt                         >  2:5541544/1‑93 (MQ=255)
                                                                  gtgtAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGa                       <  2:2032651/93‑1 (MQ=255)
                                                                  gtgtAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGa                       <  1:4995549/93‑1 (MQ=255)
                                                                   tgtAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGGATCGTTCGTTGCGACCTGAt                      >  2:4074205/1‑93 (MQ=255)
                                                                    ggaaCCTAAGCGTGCAAAAGCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATc                     <  2:3016017/91‑1 (MQ=255)
                                                                     tAACCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCg                    <  2:3664443/93‑1 (MQ=255)
                                                                      aaCCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGc                   >  2:3378381/1‑93 (MQ=255)
                                                                       aCCGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCt                  >  1:2978948/1‑93 (MQ=255)
                                                                        ccGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTg                 >  2:350591/1‑93 (MQ=255)
                                                                         cGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGa                >  1:3191800/1‑93 (MQ=255)
                                                                         cGAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGa                >  1:1421526/1‑93 (MQ=255)
                                                                          gAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGCTCGCTGAc               >  1:112465/1‑93 (MQ=255)
                                                                          gAAGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGAc               <  1:4317807/93‑1 (MQ=255)
                                                                           aaGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACg              <  2:3778818/93‑1 (MQ=255)
                                                                           aaGCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACg              >  2:2670677/1‑93 (MQ=255)
                                                                            aGCGTTTAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACgg             >  1:4932352/1‑93 (MQ=255)
                                                                             gCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGt            >  1:3656795/1‑93 (MQ=255)
                                                                             gCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGt            >  1:349977/1‑93 (MQ=255)
                                                                             gCGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGt            <  1:6578895/93‑1 (MQ=255)
                                                                              cGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTa           >  1:1683999/1‑93 (MQ=255)
                                                                              cGTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTa           <  2:6419564/93‑1 (MQ=255)
                                                                               gTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTat          <  2:5658080/93‑1 (MQ=255)
                                                                               gTTCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTat          >  2:2955778/1‑93 (MQ=255)
                                                                               gTTCAAAATAACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTat          >  1:5912240/1‑93 (MQ=255)
                                                                                 tCAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTatca        <  2:5027438/93‑1 (MQ=255)
                                                                                  cAAAATCACCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTatcat       <  2:2520144/93‑1 (MQ=255)
                                                                                      atcaccCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGTGACCTGATCGCTGACGGTATCATCGgc   >  1:1035727/1‑93 (MQ=255)
                                                                                      atcaccCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCggg   >  1:1883516/1‑92 (MQ=255)
                                                                                          |                                                                                         
TCGTTAAACTGCACGGCGGCGAACCGGCTAACTTCCTTGACGTTGGCGGCGGCGCAACCAAAGAACGTGTAACCGAAGCGTTCAAAATCATCCTCTCTGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCG  >  NC_000913/763060‑763239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑