BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	832,142	A→G	V11A (GTC→GCC)	ybiA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	832,142	0	A	G	99.4%	567.6 / ‑4.7	176	V11A (GTC→GCC)	ybiA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (83/92); total (84/92)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

Only 100 of 177 total aligned reads displayed.

CGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAT  >  NC_000913/832053‑832230

 gTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCgtg                                                                                      >  2:1657744/1‑93 (MQ=255)

  tCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCgtgt                                                                                     <  2:4960154/93‑1 (MQ=255)

   cAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGtt                                                                                    >  2:1766672/1‑93 (MQ=255)

     aCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTgg                                                                                  <  1:2264977/93‑1 (MQ=255)

      ccTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGa                                                                                 <  2:1114671/93‑1 (MQ=255)

      ccTTGATGGGCCAGGAGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGa                                                                                 <  2:5533183/93‑1 (MQ=255)

        ttGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATc                                                                               >  1:6670006/1‑93 (MQ=255)

          gATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCt                                                                             >  1:4074156/1‑93 (MQ=255)

           aTGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCtt                                                                            >  2:4468300/1‑93 (MQ=255)

           aTGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCtt                                                                            <  2:664647/93‑1 (MQ=255)

            tGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCttt                                                                           <  2:575809/93‑1 (MQ=255)

               gCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTtgtg                                                                        >  2:5096233/1‑93 (MQ=255)

                ccAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGc                                                                       <  2:4004215/93‑1 (MQ=255)

                 cAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCt                                                                      <  2:3577077/93‑1 (MQ=255)

                 cAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCt                                                                      >  1:6269272/1‑93 (MQ=255)

                      ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAAc                                                                 <  1:1364653/93‑1 (MQ=255)

                      ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAAc                                                                 >  2:4792573/1‑93 (MQ=255)

                      ggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAACTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTAGAAc                                                                 >  1:1468986/1‑93 (MQ=255)

                       gcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACg                                                                >  1:3703749/1‑93 (MQ=255)

                         aaaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACggg                                                              >  1:2542561/1‑93 (MQ=255)

                         aaaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACggg                                                              >  1:2046290/1‑93 (MQ=255)

                          aaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGc                                                             <  2:6375246/93‑1 (MQ=255)

                          aaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGc                                                             >  1:1702884/1‑93 (MQ=255)

                           aGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCa                                                            <  2:808430/93‑1 (MQ=255)

                            gTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCAt                                                           >  2:2214239/1‑93 (MQ=255)

                              tAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTa                                                         <  1:3085727/93‑1 (MQ=255)

                              tAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTa                                                         <  1:453735/93‑1 (MQ=255)

                                gAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTaaa                                                       <  1:5745940/93‑1 (MQ=255)

                                gAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTaaa                                                       >  2:3627914/1‑93 (MQ=255)

                                 aaaaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAc                                                      >  2:5908478/1‑93 (MQ=255)

                                   aaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCg                                                    >  1:1569396/1‑93 (MQ=255)

                                     aTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCa                                                  >  1:5287204/1‑93 (MQ=255)

                                     aTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCa                                                  <  1:5085180/93‑1 (MQ=255)

                                      tCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAt                                                 >  2:4923993/1‑93 (MQ=255)

                                       cACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAtt                                                <  2:6227926/93‑1 (MQ=255)

                                        aCCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAGCGGGCATTAAACCGCATTa                                               >  1:5995597/1‑93 (MQ=255)

                                        aCCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTa                                               >  1:4316756/1‑93 (MQ=255)

                                         ccGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTAt                                              <  2:676155/93‑1 (MQ=255)

                                         ccGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTAt                                              >  1:2290526/1‑93 (MQ=255)

                                          cGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATg                                             <  1:3968801/93‑1 (MQ=255)

                                          cGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATg                                             <  2:2002273/93‑1 (MQ=255)

                                           gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGt                                            >  2:1964987/1‑93 (MQ=255)

                                           gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGt                                            >  1:5536343/1‑93 (MQ=255)

                                           gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGt                                            >  1:6044424/1‑93 (MQ=255)

                                           gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGt                                            >  1:3018427/1‑93 (MQ=255)

                                           gTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGt                                            >  2:357345/1‑93 (MQ=255)

                                            tAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGtt                                           <  1:17391/93‑1 (MQ=255)

                                             aGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtg                                          >  1:4465252/1‑93 (MQ=255)

                                              gTCATCTCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTGGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtgg                                         <  1:192378/93‑1 (MQ=255)

                                              gTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtgg                                         >  1:5377013/1‑93 (MQ=255)

                                              gTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtgg                                         <  2:5867267/93‑1 (MQ=255)

                                              gTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtgg                                         >  2:6116085/1‑93 (MQ=255)

                                               tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAGCCGCATTATGTtggt                                        <  1:430913/93‑1 (MQ=255)

                                               tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggt                                        >  2:4171182/1‑93 (MQ=255)

                                               tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggt                                        >  1:4895031/1‑93 (MQ=255)

                                               tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggt                                        <  2:2533678/93‑1 (MQ=255)

                                                cATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtg                                       >  1:1091360/1‑93 (MQ=255)

                                                 aTCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtgg                                      >  2:1280645/1‑93 (MQ=255)

                                                 aTCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtgg                                      >  1:5028021/1‑93 (MQ=255)

                                                 aTCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtgg                                      >  1:1159281/1‑93 (MQ=255)

                                                  tCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTggtggt                                     >  1:365337/1‑93 (MQ=255)

                                                  tCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTggtggt                                     >  2:3344316/1‑93 (MQ=255)

                                                   cGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGtt                                    <  2:117131/93‑1 (MQ=255)

                                                   cGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGtt                                    <  1:1076587/93‑1 (MQ=255)

                                                    gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCGTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTa                                   >  1:6624375/1‑93 (MQ=255)

                                                    gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTa                                   <  1:698979/93‑1 (MQ=255)

                                                    gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTa                                   <  2:3242945/93‑1 (MQ=255)

                                                     cTGGTGCTGTAGAACTTTATGATGGTGTCTTGCATGGCGTGTTGGATCCCTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAt                                  <  2:1676745/93‑1 (MQ=255)

                                                     cTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAt                                  <  2:6380516/93‑1 (MQ=255)

                                                      tGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                 <  1:4268552/93‑1 (MQ=255)

                                                          gCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGa                             <  2:539129/93‑1 (MQ=255)

                                                          gCTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGa                             >  1:5456313/1‑93 (MQ=255)

                                                           cTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAg                            <  2:2681141/93‑1 (MQ=255)

                                                           cTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAg                            <  1:670804/93‑1 (MQ=255)

                                                           cTGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAg                            <  1:5601576/93‑1 (MQ=255)

                                                            tGTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGc                           >  1:4657252/1‑93 (MQ=255)

                                                             gTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGcc                          <  2:1958365/93‑1 (MQ=255)

                                                             gTAGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGcc                          >  1:4853661/1‑93 (MQ=255)

                                                              tAGAAATTTATGATGGTGTCTTACATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCg                         <  1:1974586/93‑1 (MQ=255)

                                                               aGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGc                        >  2:2476372/1‑93 (MQ=255)

                                                               aGAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGc                        <  2:1797616/93‑1 (MQ=255)

                                                                gAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCt                       >  1:3609993/1‑93 (MQ=255)

                                                                gAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCt                       <  2:6280725/93‑1 (MQ=255)

                                                                gAAATTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCt                       >  1:2996704/1‑93 (MQ=255)

                                                                  aaTTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCttt                     <  1:1835301/93‑1 (MQ=255)

                                                                   aTTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTc                    <  1:6083669/93‑1 (MQ=255)

                                                                   aTTTATGATGGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTc                    >  2:6496142/1‑93 (MQ=255)

                                                                       atgatgGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcaga                <  2:1487425/93‑1 (MQ=255)

                                                                        tgatgGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaa               >  2:5588788/1‑93 (MQ=255)

                                                                        tgatgGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaa               >  2:4369326/1‑93 (MQ=255)

                                                                         gatgGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaa              >  2:6692383/1‑93 (MQ=255)

                                                                         gatgGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaa              >  1:1458223/1‑93 (MQ=255)

                                                                          atgGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaac             <  1:4037929/93‑1 (MQ=255)

                                                                          atgGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaac             <  1:976232/93‑1 (MQ=255)

                                                                          atgGTGTCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaac             >  2:898092/1‑93 (MQ=255)

                                                                            ggggtCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacag           >  1:2996997/4‑93 (MQ=255)

                                                                              tgtCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaacagaa         >  1:2696545/1‑93 (MQ=255)

                                                                                tCTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGaaaa       <  2:6334176/93‑1 (MQ=255)

                                                                                 cTTGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGaaaaa      >  2:6556277/1‑93 (MQ=255)

                                                                                  ttGCATGGCGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAAc     >  2:4203049/1‑93 (MQ=255)

CGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAT  >  NC_000913/832053‑832230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`