BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	862,272	T→C	T164A (ACT→GCT)	ybiY ←	putative pyruvate formate‑lyase activating enzyme 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	862,272	0	T	C	100.0%	648.4 / NA	197	T164A (ACT→GCT)	ybiY	putative pyruvate formate‑lyase activating enzyme 2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (112/85); total (112/85)

Only 100 of 205 total aligned reads displayed.

CGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGC  >  NC_000913/862182‑862361

  tgtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGa                                                                                       >  2:227800/1‑93 (MQ=255)

    ttttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAt                                                                                     <  1:1847427/93‑1 (MQ=255)

     tttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtg                                                                                    <  1:3568565/93‑1 (MQ=255)

        aaaTCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgcct                                                                                 <  1:6600319/93‑1 (MQ=255)

         aaTCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGGTTCTACCGCAGCATGAAtgcctg                                                                                >  1:6346179/1‑93 (MQ=255)

          aTCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgcctgc                                                                               <  2:6152781/93‑1 (MQ=255)

           tCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgcctgcc                                                                              <  2:4435075/93‑1 (MQ=255)

            cGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATgcctgcct                                                                             <  1:1063938/93‑1 (MQ=255)

            cGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATgcctgcct                                                                             >  2:5519517/1‑93 (MQ=255)

            cGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATgcctgcct                                                                             >  2:3364645/1‑93 (MQ=255)

             ggCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTc                                                                            >  1:3443526/1‑93 (MQ=255)

             ggCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTc                                                                            >  2:3740093/1‑93 (MQ=255)

              gCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCg                                                                           <  2:3024443/93‑1 (MQ=255)

              gCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCg                                                                           <  1:6200798/93‑1 (MQ=255)

               gaaGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGt                                                                          <  2:4331142/92‑1 (MQ=255)

               cAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGt                                                                          <  2:2863251/93‑1 (MQ=255)

                aaGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtg                                                                         >  1:5086023/1‑93 (MQ=255)

                aaGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtg                                                                         >  1:4891955/1‑93 (MQ=255)

                aaGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtg                                                                         >  1:1963283/1‑93 (MQ=255)

                 aGAAACAGATCGATATAGGGCAGAGACGGGGCGATATATTTCCACGGCACATGCAGACAGGGTTCTACCGAAGCATGAATGCCTGCCTCGtgg                                                                        >  2:1006582/1‑93 (MQ=255)

                   aaaCAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggct                                                                      >  1:4198364/1‑93 (MQ=255)

                     aCAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggctgg                                                                    >  2:4047263/1‑93 (MQ=255)

                       aGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTggctggct                                                                  >  2:6682089/1‑93 (MQ=255)

                       aGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTggctggct                                                                  <  1:6150317/93‑1 (MQ=255)

                       aGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTggctggct                                                                  <  1:2493686/93‑1 (MQ=255)

                        gATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCtt                                                                 <  2:3126019/93‑1 (MQ=255)

                         aTCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTg                                                                >  1:484864/1‑93 (MQ=255)

                           cGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCa                                                              <  1:2791137/93‑1 (MQ=255)

                           cGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCa                                                              <  1:2987008/93‑1 (MQ=255)

                            gATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAg                                                             <  2:2811094/93‑1 (MQ=255)

                            gATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAg                                                             >  1:731593/1‑93 (MQ=255)

                            gATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAg                                                             >  2:3233912/1‑93 (MQ=255)

                             atatAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGt                                                            >  1:3843374/1‑93 (MQ=255)

                              tataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTa                                                           >  2:1235812/1‑93 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          <  1:3097867/93‑1 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          >  1:6065989/1‑93 (MQ=255)

                                taGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgc                                                         <  1:2702716/93‑1 (MQ=255)

                                taGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgc                                                         <  1:6318006/93‑1 (MQ=255)

                                 agcgCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGGTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCTGTAgcg                                                        >  1:6398107/4‑93 (MQ=255)

                                  gggCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc                                                       >  2:1724306/1‑93 (MQ=255)

                                  gggCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc                                                       <  1:4381961/93‑1 (MQ=255)

                                     cAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccat                                                    >  2:1056000/1‑93 (MQ=255)

                                      agagAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatc                                                   <  1:1069234/93‑1 (MQ=255)

                                       gagaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcg                                                  >  2:5854368/1‑93 (MQ=255)

                                        agaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGggccatcgc                                                 >  2:1042376/1‑93 (MQ=255)

                                         gaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgcc                                                >  1:465251/1‑93 (MQ=255)

                                         gaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgcc                                                >  1:2351712/1‑93 (MQ=255)

                                          aaGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgcca                                               >  1:5182823/1‑93 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              >  2:4604304/1‑93 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              <  2:464392/93‑1 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              <  2:5753729/93‑1 (MQ=255)

                                            ggggCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCAtt                                             >  2:1681954/1‑93 (MQ=255)

                                            ggggCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCAtt                                             >  1:5278959/1‑93 (MQ=255)

                                              ggCGATATATTTCCACGGCACATGCAGATAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTGTCAGTAGCGACATCGCCATTTc                                           <  1:146530/93‑1 (MQ=255)

                                                cGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCg                                         >  2:3079831/1‑93 (MQ=255)

                                                 gATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCgg                                        >  1:4319546/1‑93 (MQ=255)

                                                 gATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCgg                                        >  2:3937584/1‑93 (MQ=255)

                                                  atatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc                                       <  2:4793663/93‑1 (MQ=255)

                                                  atatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAACGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGc                                       >  2:145591/1‑93 (MQ=255)

                                                       tttCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCAt                                  >  1:837867/1‑93 (MQ=255)

                                                       tttCCACGGCACATGCAGACAGGTTTCTAACGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGcca                                  >  1:2186777/1‑91 (MQ=255)

                                                        ttCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATa                                 <  2:2652701/93‑1 (MQ=255)

                                                         tCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaa                                >  2:4021480/1‑93 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATTGCCATTTCCGGCTGCATaaa                               >  1:2901315/1‑93 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCTATTTCCGGCTGCATaaa                               >  1:1730755/1‑93 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               <  2:3335473/93‑1 (MQ=255)

                                                           cACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGACTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAg                              <  2:4696195/93‑1 (MQ=255)

                                                            aCGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGGCATTTCCGGCTGCATAAAgg                             >  1:4137085/1‑93 (MQ=255)

                                                            aCGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaaag                             >  1:4943581/1‑91 (MQ=255)

                                                            aCGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAgg                             >  2:1953927/1‑93 (MQ=255)

                                                            aCGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAgg                             <  1:5942080/93‑1 (MQ=255)

                                                            aCGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAgg                             <  1:3166432/93‑1 (MQ=255)

                                                              ggCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGc                           <  2:4156995/93‑1 (MQ=255)

                                                               gCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCt                          <  2:277139/93‑1 (MQ=255)

                                                                cacaTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTc                         <  1:3226124/93‑1 (MQ=255)

                                                                 acaTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGGCTAAAAGGCTca                        >  1:1192771/1‑93 (MQ=255)

                                                                 acaTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTca                        >  2:3606487/1‑93 (MQ=255)

                                                                   aTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacc                      >  2:6232409/1‑93 (MQ=255)

                                                                   aTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacc                      <  2:4091737/93‑1 (MQ=255)

                                                                   aTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacc                      <  2:4466680/93‑1 (MQ=255)

                                                                    tGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTcacca                     <  2:6551191/93‑1 (MQ=255)

                                                                       aGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCAccc                  >  1:5609993/1‑93 (MQ=255)

                                                                         aCAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGa                <  1:3311509/93‑1 (MQ=255)

                                                                           aGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGaaa              <  2:1391536/93‑1 (MQ=255)

                                                                            ggTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAg             >  1:2488752/1‑93 (MQ=255)

                                                                            ggTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAg             >  2:5296243/1‑93 (MQ=255)

                                                                            ggTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAg             <  1:3020097/93‑1 (MQ=255)

                                                                               ttCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGt          <  1:3464884/93‑1 (MQ=255)

                                                                                tCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGtt         <  2:4191166/93‑1 (MQ=255)

                                                                                 cTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCGTAAAGGGCTCACCACCCGAAAGCGTTa        >  2:1302637/1‑93 (MQ=255)

                                                                                 cTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTa        <  1:4963124/93‑1 (MQ=255)

                                                                                 cTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTa        >  1:3861530/1‑93 (MQ=255)

                                                                                  tACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTaa       >  1:5793692/1‑93 (MQ=255)

                                                                                   aCCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGGTaaa      >  1:5428199/1‑93 (MQ=255)

                                                                                    ccGCGGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAc     <  2:6635759/93‑1 (MQ=255)

                                                                                     cGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAcc    >  2:1578346/1‑93 (MQ=255)

                                                                                     cGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAcc    <  2:536672/93‑1 (MQ=255)

                                                                                      gcagcaTGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAccg   >  2:2615529/1‑93 (MQ=255)

                                                                                      gcagcaTGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAccg   >  2:4729920/1‑93 (MQ=255)

                                                                                       cAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAAccgc  <  2:1882486/93‑1 (MQ=255)

CGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGC  >  NC_000913/862182‑862361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`