BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	974,364	A→G	I275V (ATT→GTT)	mukF →	MukF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	974,364	0	A	G	99.1%	719.7 / ‑4.8	225	I275V (ATT→GTT)	mukF	MukF
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (113/110); minor base T (1/1); total (114/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG  >  NC_000913/974274‑974453

gATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGtt                                                                                         >  2:5241689/1‑93 (MQ=255)

   cTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGc                                                                                      <  1:1068685/93‑1 (MQ=255)

   cTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGc                                                                                      <  1:1327901/93‑1 (MQ=255)

     gCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTa                                                                                    >  2:830969/1‑93 (MQ=255)

       aTTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACg                                                                                  >  2:1262863/1‑93 (MQ=255)

       aTTTCGTCGATCGTCTGGGGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCCACg                                                                                  >  1:4781425/1‑93 (MQ=255)

        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGa                                                                                 <  2:4629753/93‑1 (MQ=255)

         ttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAc                                                                                <  1:2674242/93‑1 (MQ=255)

         ttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAc                                                                                <  1:2250764/93‑1 (MQ=255)

               gATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCTTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCAc                                                                          <  1:3731987/93‑1 (MQ=255)

                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                         >  2:353754/1‑93 (MQ=255)

                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                         >  2:682292/1‑93 (MQ=255)

                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                         >  1:3182450/1‑93 (MQ=255)

                 tCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGt                                                                        >  2:5338862/1‑93 (MQ=255)

                    tCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTaca                                                                     <  1:815336/93‑1 (MQ=255)

                    tCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTaca                                                                     >  2:5784409/1‑93 (MQ=255)

                      tGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAcaca                                                                   >  1:5870104/1‑93 (MQ=255)

                          gtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAtt                                                               >  2:5444442/1‑93 (MQ=255)

                          gtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAtt                                                               <  2:751429/93‑1 (MQ=255)

                          gtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAtt                                                               >  1:4259007/1‑93 (MQ=255)

                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                              <  2:627421/93‑1 (MQ=255)

                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                              <  2:895710/93‑1 (MQ=255)

                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                              <  2:4456857/93‑1 (MQ=255)

                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                              >  2:4360720/1‑93 (MQ=255)

                            tCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTa                                                             <  2:2188974/93‑1 (MQ=255)

                             tgATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTAt                                                            <  2:5383802/92‑1 (MQ=255)

                             cGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTAt                                                            <  2:5559695/93‑1 (MQ=255)

                              gATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTAtt                                                           <  1:2611829/93‑1 (MQ=255)

                              gATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTAtt                                                           <  1:2412060/93‑1 (MQ=255)

                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                         <  1:3366789/93‑1 (MQ=255)

                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                         <  2:4936057/93‑1 (MQ=255)

                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACAGCCACGTACACAAATTTATTCg                                                         >  1:6483045/1‑93 (MQ=255)

                                 ttGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCAAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGt                                                        <  1:4300557/92‑1 (MQ=255)

                                 cggCAGAGCAAACGCGATCGTATTATCAGTGGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGt                                                        <  2:3449834/91‑1 (MQ=255)

                                 cTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGTTTGGCTACGACCGCCACGTACACAAATTTATTCGt                                                        >  2:1604121/1‑93 (MQ=255)

                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                       >  2:2403920/1‑93 (MQ=255)

                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                       >  2:5618466/1‑93 (MQ=255)

                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                       <  2:2437349/93‑1 (MQ=255)

                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                       >  2:3769080/1‑93 (MQ=255)

                                   gCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAc                                                      <  1:2975795/93‑1 (MQ=255)

                                     agagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcg                                                    >  2:1000400/1‑93 (MQ=255)

                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                   >  2:6306574/1‑93 (MQ=255)

                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                   >  1:1411101/1‑93 (MQ=255)

                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                   >  1:3479625/1‑93 (MQ=255)

                                       agCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgcg                                                  >  1:6660591/1‑93 (MQ=255)

                                        gCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcga                                                 >  1:6385809/1‑93 (MQ=255)

                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTTTGGTTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                                <  1:1153572/93‑1 (MQ=255)

                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                                <  1:4910810/93‑1 (MQ=255)

                                           aaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcg                                              >  1:1593636/1‑93 (MQ=255)

                                           aaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcg                                              >  2:3283952/1‑93 (MQ=255)

                                             cTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcgat                                            <  1:890747/93‑1 (MQ=255)

                                             cTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcgat                                            <  1:3904675/93‑1 (MQ=255)

                                                gATCGTATTATCAGTTGTGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATg                                         <  1:6006971/93‑1 (MQ=255)

                                                gATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATg                                         <  1:2127775/93‑1 (MQ=255)

                                                 aTCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGACTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATgg                                        >  1:5204933/1‑93 (MQ=255)

                                                    gTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATa                                     >  1:4141546/1‑93 (MQ=255)

                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                   <  2:6635849/93‑1 (MQ=255)

                                                       ttatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaa                                  >  2:1220701/1‑93 (MQ=255)

                                                       ttatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaa                                  <  2:3194691/93‑1 (MQ=255)

                                                       ttatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaa                                  >  1:1472310/1‑93 (MQ=255)

                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaaa                                 <  1:1243852/93‑1 (MQ=255)

                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaaa                                 >  1:5012459/1‑93 (MQ=255)

                                                         atCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAc                                <  2:2540250/93‑1 (MQ=255)

                                                          tCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAcc                               <  2:1753654/93‑1 (MQ=255)

                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                            >  1:4451155/1‑93 (MQ=255)

                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGCACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                            >  2:6192059/1‑93 (MQ=255)

                                                               tGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTc                          <  2:6570217/93‑1 (MQ=255)

                                                                ggggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCt                         <  2:1675198/93‑1 (MQ=255)

                                                                 gggTCAGTATTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCtt                        <  1:2489013/93‑1 (MQ=255)

                                                                 gggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCtt                        <  1:3038217/93‑1 (MQ=255)

                                                                  ggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCttt                       <  1:6035193/93‑1 (MQ=255)

                                                                   gCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTg                      >  1:3253632/1‑93 (MQ=255)

                                                                    ccAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGc                     >  1:4685190/1‑93 (MQ=255)

                                                                    ccAGCAATCCAGCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGc                     <  2:2864207/93‑1 (MQ=255)

                                                                     cAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCt                    <  1:100810/93‑1 (MQ=255)

                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                   >  2:5912763/1‑93 (MQ=255)

                                                                       gCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCa                  >  2:4080588/1‑93 (MQ=255)

                                                                       gCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCa                  >  2:1642077/1‑93 (MQ=255)

                                                                       gCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCa                  >  1:6537973/1‑93 (MQ=255)

                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc                >  2:1871957/1‑93 (MQ=255)

                                                                          aTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGGTATGGATAAAAACCGCGTCTTTGCTCAGCg               >  2:4220689/1‑93 (MQ=255)

                                                                          aTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCg               <  1:4848600/93‑1 (MQ=255)

                                                                          aTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCg               >  2:1502983/1‑93 (MQ=255)

                                                                           tCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCgg              >  1:1129779/1‑93 (MQ=255)

                                                                           tCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCgg              >  2:6408714/1‑93 (MQ=255)

                                                                            ccATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGt             >  1:2700625/1‑93 (MQ=255)

                                                                                cGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACg         <  1:5550774/93‑1 (MQ=255)

                                                                                cGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACg         <  2:1900139/93‑1 (MQ=255)

                                                                                 gACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt        >  1:3944301/1‑93 (MQ=255)

                                                                                 gACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt        <  1:5690394/93‑1 (MQ=255)

                                                                                 gACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt        >  1:839053/1‑93 (MQ=255)

                                                                                 gACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt        <  2:5855311/93‑1 (MQ=255)

                                                                                 gACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt        <  1:1789883/93‑1 (MQ=255)

                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc       >  1:542413/1‑93 (MQ=255)

                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc       <  2:2685247/93‑1 (MQ=255)

                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc       <  2:2970593/93‑1 (MQ=255)

                                                                                   cTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCa      >  2:1116920/1‑93 (MQ=255)

                                                                                    ttGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAg     <  2:4913886/93‑1 (MQ=255)

                                                                                     tgtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGt    >  2:2672417/1‑93 (MQ=255)

                                                                                       tgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCg  >  1:6198876/1‑93 (MQ=255)

GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTCG  >  NC_000913/974274‑974453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`