BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,435	A→G	S52S (AGT→AGC)	rne ←	RNase E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,435	0	A	G	99.6%	748.0 / NA	230	S52S (AGT→AGC)	rne	RNase E
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (123/106); minor base C (1/0); total (124/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

GTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACG  >  NC_000913/1143345‑1143524

gTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCATCTTCCAGGCt                                                                                         <  1:2614717/93‑1 (MQ=255)

gTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTGTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCt                                                                                         <  2:6219879/93‑1 (MQ=255)

  aGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCg                                                                                       >  2:3102231/1‑93 (MQ=255)

  aGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCg                                                                                       <  2:4215102/93‑1 (MQ=255)

  aGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCg                                                                                       >  1:1373346/1‑93 (MQ=255)

    ttAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGt                                                                                     >  2:5795916/1‑93 (MQ=255)

      aGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTc                                                                                   <  2:3733854/93‑1 (MQ=255)

      aGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTc                                                                                   <  1:1276700/93‑1 (MQ=255)

       gCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCa                                                                                  >  1:3169258/1‑93 (MQ=255)

       gCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCa                                                                                  >  2:3424166/1‑93 (MQ=255)

         aGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAAt                                                                                <  2:143906/93‑1 (MQ=255)

         aGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAAt                                                                                <  1:3308943/93‑1 (MQ=255)

          gggAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATg                                                                               >  1:6468383/1‑93 (MQ=255)

           ggAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGc                                                                              <  1:4524671/93‑1 (MQ=255)

            gAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCg                                                                             >  1:3783753/1‑93 (MQ=255)

             aaaTATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCgg                                                                            <  2:1342463/93‑1 (MQ=255)

             aaaTATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCgg                                                                            <  1:1587093/93‑1 (MQ=255)

              aaTATTCTTGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCATCGCCGTAATCAACTAATTCAGCTTCCAGGCTGGGTTCAATGCggg                                                                           >  2:6722963/1‑93 (MQ=255)

              aaTATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCggg                                                                           <  1:624016/93‑1 (MQ=255)

              aaTATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCggg                                                                           >  1:233159/1‑93 (MQ=255)

              aaTATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCggg                                                                           <  1:2217531/93‑1 (MQ=255)

                tatTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTg                                                                         >  1:6034127/1‑93 (MQ=255)

                tatTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTg                                                                         >  1:1676759/1‑93 (MQ=255)

                  ttCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAt                                                                       >  1:2854232/1‑93 (MQ=255)

                  ttCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAt                                                                       <  2:3672286/93‑1 (MQ=255)

                  ttCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAt                                                                       <  1:3413269/93‑1 (MQ=255)

                  ttCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAt                                                                       >  1:3995055/1‑93 (MQ=255)

                   tCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAtt                                                                      <  2:6381106/93‑1 (MQ=255)

                    cgcgGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGAttt                                                                     >  1:1094398/1‑93 (MQ=255)

                      cgGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTa                                                                   >  2:3817877/1‑93 (MQ=255)

                       gggCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTAc                                                                  >  1:5072496/1‑93 (MQ=255)

                       gggCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTAc                                                                  >  2:6009297/1‑93 (MQ=255)

                        ggCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTAcc                                                                 >  2:5851725/1‑93 (MQ=255)

                         gCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCt                                                                <  2:1745661/93‑1 (MQ=255)

                         gCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCt                                                                <  2:4619724/93‑1 (MQ=255)

                         gCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCt                                                                <  2:3432819/93‑1 (MQ=255)

                         gCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCt                                                                >  1:2448324/1‑93 (MQ=255)

                            aTTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTg                                                             >  1:945137/1‑93 (MQ=255)

                               tCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAg                                                          <  2:5297222/93‑1 (MQ=255)

                               tCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAg                                                          >  1:5558081/1‑93 (MQ=255)

                                 ttttAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGAt                                                        >  1:4407726/1‑93 (MQ=255)

                                 ttttAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGAt                                                        <  2:1512789/93‑1 (MQ=255)

                                 ttttAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGAt                                                        <  2:1054520/93‑1 (MQ=255)

                                 ttttAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGAt                                                        >  1:2705566/1‑93 (MQ=255)

                                 ttttAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGCCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGAt                                                        >  2:867230/1‑93 (MQ=255)

                                  tttAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATg                                                       <  1:3669434/93‑1 (MQ=255)

                                      gTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTg                                                   >  1:1995031/1‑93 (MQ=255)

                                        gggAGGAAACCGTGACGTTTAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGcc                                                 >  1:6571263/1‑93 (MQ=255)

                                          gaggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCTGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCtt                                               <  1:5433690/93‑1 (MQ=255)

                                           aggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCttt                                              <  2:2642233/93‑1 (MQ=255)

                                           aggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCttt                                              <  2:1774441/93‑1 (MQ=255)

                                           aggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCttt                                              <  1:5121043/93‑1 (MQ=255)

                                            ggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGTTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCtttt                                             >  1:3302401/1‑93 (MQ=255)

                                            ggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCtttt                                             >  2:4384227/1‑93 (MQ=255)

                                              aaaCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTc                                           <  2:1498826/93‑1 (MQ=255)

                                              aaaCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTc                                           <  1:3840090/93‑1 (MQ=255)

                                               aaCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCt                                          <  2:271517/93‑1 (MQ=255)

                                               aaCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGACTTTACCTTTGTAGATGTTTGCCTTTTTCt                                          >  1:6282042/1‑93 (MQ=255)

                                                aCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTg                                         >  2:2204703/1‑93 (MQ=255)

                                                 ccGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGc                                        <  2:5618944/93‑1 (MQ=255)

                                                   gTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGGCTTTTTCTtctc                                      >  2:2298088/1‑93 (MQ=255)

                                                   gTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTc                                      <  2:642923/93‑1 (MQ=255)

                                                   gTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTc                                      >  1:1268250/1‑93 (MQ=255)

                                                     gACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGt                                    >  2:2335071/1‑93 (MQ=255)

                                                     gACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGt                                    >  1:4921712/1‑93 (MQ=255)

                                                     gACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGt                                    >  1:6082127/1‑93 (MQ=255)

                                                     gACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGt                                    >  2:1252062/1‑93 (MQ=255)

                                                       cGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGc                                  >  1:2652967/1‑93 (MQ=255)

                                                       cGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGc                                  >  1:2942416/1‑93 (MQ=255)

                                                        gTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGcc                                 <  2:1934537/93‑1 (MQ=255)

                                                        gTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGcc                                 <  2:3210522/93‑1 (MQ=255)

                                                         ttCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGccc                                >  1:6730527/1‑93 (MQ=255)

                                                         ttCAGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGccc                                <  2:5694038/93‑1 (MQ=255)

                                                            aGCGCCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTgg                             >  2:5080266/1‑93 (MQ=255)

                                                              cgcCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGAc                           >  2:6674651/1‑93 (MQ=255)

                                                               gcCGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACt                          <  2:5472165/93‑1 (MQ=255)

                                                                 cGTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACttt                        >  2:299786/1‑93 (MQ=255)

                                                                  gTAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTc                       <  2:116646/93‑1 (MQ=255)

                                                                   tAATCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCg                      >  1:1902967/1‑93 (MQ=255)

                                                                    aaTCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGa                     >  2:4320130/1‑93 (MQ=255)

                                                                    aaTCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGa                     >  1:4239208/1‑93 (MQ=255)

                                                                     aTCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGat                    >  2:4124338/1‑93 (MQ=255)

                                                                     aTCAACAAAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATGTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGat                    >  2:4693194/1‑93 (MQ=255)

                                                                         acaaAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATcc                <  1:486056/93‑1 (MQ=255)

                                                                         acaaAAGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATcc                >  1:2105362/1‑93 (MQ=255)

                                                                           aaaaGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTATAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAg              <  1:1835314/93‑1 (MQ=255)

                                                                            aaaGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAgg             >  1:3553684/1‑93 (MQ=255)

                                                                            aaaGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAgg             <  2:6330972/93‑1 (MQ=255)

                                                                             aaGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGt            <  2:399795/93‑1 (MQ=255)

                                                                             aaGCAGCTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGt            >  2:248650/1‑93 (MQ=255)

                                                                              agcagcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTc           >  2:416508/1‑93 (MQ=255)

                                                                              agcagcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTc           <  2:5330323/93‑1 (MQ=255)

                                                                              agcagcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTc           >  2:5094880/1‑93 (MQ=255)

                                                                                 agcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATa        >  1:6088472/1‑93 (MQ=255)

                                                                                  gcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATAc       >  2:495151/1‑93 (MQ=255)

                                                                                  gcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATAc       >  1:2548530/1‑93 (MQ=255)

                                                                                  gcTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATAc       >  2:2111805/1‑93 (MQ=255)

                                                                                   cTTCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACa      <  1:5933742/93‑1 (MQ=255)

                                                                                    ttCCAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAg     >  1:4094542/1‑93 (MQ=255)

                                                                                       cAGGCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACg  >  2:3503915/1‑93 (MQ=255)

GTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACG  >  NC_000913/1143345‑1143524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`