BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,184,484	A→G	F112L (TTT→CTT)	potA ←	ATP‑binding component of spermidine/putrescine transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,184,484	0	A	G	100.0%	709.1 / NA	220	F112L (TTT→CTT)	potA	ATP‑binding component of spermidine/putrescine transport
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (94/126); total (94/126)

Only 100 of 229 total aligned reads displayed.

GAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGG  >  NC_000913/1184394‑1184573

gAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGGTTTTTGCATGCGCAACCCAAggg                                                                                         >  1:5798495/1‑93 (MQ=255)

 aGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGc                                                                                        <  1:5385195/93‑1 (MQ=255)

 aGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGc                                                                                        >  1:3155741/1‑93 (MQ=255)

 aGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGc                                                                                        >  1:6001450/1‑93 (MQ=255)

  gCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGcc                                                                                       <  2:6141149/93‑1 (MQ=255)

    gAAGGTTTCCAACTGCACCATCCGCAGGGCGTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCcac                                                                                     >  2:3338493/1‑93 (MQ=255)

     aaGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCcaca                                                                                    <  1:512847/93‑1 (MQ=255)

     aaGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCcaca                                                                                    >  1:1722226/1‑93 (MQ=255)

     aaGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCcaca                                                                                    <  2:1749704/93‑1 (MQ=255)

      aGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGTGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACAt                                                                                   >  1:276614/1‑93 (MQ=255)

      aGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACAt                                                                                   >  1:497987/1‑93 (MQ=255)

      aGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATATCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACAt                                                                                   >  1:6624172/1‑93 (MQ=255)

        gTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACAttt                                                                                 <  1:1493869/93‑1 (MQ=255)

        gTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGGTTTTTGCATGCGCAACCCAAGGGGCACAttt                                                                                 >  1:507213/1‑93 (MQ=255)

         tttCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACAtttt                                                                                <  2:212022/93‑1 (MQ=255)

         tttCAAACTGCACGATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACAtttt                                                                                <  1:1366347/93‑1 (MQ=255)

          ttCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTc                                                                               >  2:5683259/1‑93 (MQ=255)

          ttCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTc                                                                               <  1:2059799/93‑1 (MQ=255)

             cAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGaa                                                                            >  2:483400/1‑93 (MQ=255)

              aaCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAac                                                                           >  1:3960902/1‑93 (MQ=255)

              aaCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAac                                                                           >  1:1234721/1‑93 (MQ=255)

               aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAaca                                                                          <  1:2388668/93‑1 (MQ=255)

                cTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAacac                                                                         <  1:65017/93‑1 (MQ=255)

                   cACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGt                                                                      <  1:6408612/93‑1 (MQ=255)

                    aCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTc                                                                     >  1:2825336/1‑93 (MQ=255)

                    aCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTc                                                                     <  1:1418361/93‑1 (MQ=255)

                      cATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAt                                                                   <  2:23424/93‑1 (MQ=255)

                        tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAtgt                                                                 <  2:1039619/93‑1 (MQ=255)

                         ccGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAtgtg                                                                <  1:830965/93‑1 (MQ=255)

                         ccGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAtgtg                                                                <  1:5115552/93‑1 (MQ=255)

                          cGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCATCCCAAGGGCCACATTTTCGAACACGGTCATGTgg                                                               <  2:2568661/93‑1 (MQ=255)

                          cGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTgg                                                               <  1:4388149/93‑1 (MQ=255)

                            cAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTgggg                                                             <  2:5361762/93‑1 (MQ=255)

                            cAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTgggg                                                             >  2:4784600/1‑93 (MQ=255)

                             aGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTggggg                                                            >  2:2978349/1‑93 (MQ=255)

                             aGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTggggg                                                            >  1:3107195/1‑93 (MQ=255)

                                 cTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGaaaa                                                        <  2:1488895/93‑1 (MQ=255)

                                     cATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAgggg                                                    >  1:4902221/1‑90 (MQ=255)

                                     cATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGc                                                    <  2:3859624/93‑1 (MQ=255)

                                     cATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGc                                                    <  2:2716345/93‑1 (MQ=255)

                                      aTCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCg                                                   >  1:5608928/1‑93 (MQ=255)

                                      aTCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCg                                                   <  1:346226/93‑1 (MQ=255)

                                        cACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTa                                                 <  2:4160834/93‑1 (MQ=255)

                                        cACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTa                                                 <  2:627087/93‑1 (MQ=255)

                                          cgcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGc                                               >  2:4362541/1‑93 (MQ=255)

                                            cgGGTCGTAATTTCAGCAGCGGGTGTTTTTTGCACGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCtt                                             <  2:2316908/93‑1 (MQ=255)

                                            cgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCtt                                             >  1:3984459/1‑93 (MQ=255)

                                              gggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGCAAAGTGCGTAGCTTTg                                           >  1:4474049/1‑93 (MQ=255)

                                              gggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTg                                           <  1:744566/93‑1 (MQ=255)

                                                  gTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaaa                                       >  2:1116242/1‑93 (MQ=255)

                                                   tAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaaaa                                      <  2:2524514/93‑1 (MQ=255)

                                                    aaTTTCAGCAGCGGGTGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAAc                                     <  1:3799514/93‑1 (MQ=255)

                                                    aaTTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAAc                                     >  2:2840655/1‑93 (MQ=255)

                                                     aTTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACa                                    <  1:1080340/93‑1 (MQ=255)

                                                     aTTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACa                                    <  1:5459232/93‑1 (MQ=255)

                                                      tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACAg                                   >  2:6368096/1‑93 (MQ=255)

                                                      tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAg                                   <  2:4931752/93‑1 (MQ=255)

                                                      tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAg                                   >  1:479694/1‑93 (MQ=255)

                                                      tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAg                                   >  2:4664785/1‑93 (MQ=255)

                                                       ttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAgt                                  <  1:6355924/93‑1 (MQ=255)

                                                       ttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAgt                                  >  2:3683468/1‑93 (MQ=255)

                                                        tCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAgtg                                 <  1:1537918/93‑1 (MQ=255)

                                                         cagcagCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAgtgt                                <  2:2923355/93‑1 (MQ=255)

                                                          agcagcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGtt                               <  2:3479460/93‑1 (MQ=255)

                                                           gcagcGGTGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTc                              <  2:5962456/93‑1 (MQ=255)

                                                           gcagcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTc                              >  2:1792293/1‑93 (MQ=255)

                                                           gcagcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTc                              >  2:5133717/1‑93 (MQ=255)

                                                                gggtgTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATa                         <  1:5531863/93‑1 (MQ=255)

                                                                gggggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATa                         >  2:1583323/1‑93 (MQ=255)

                                                                 ggggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                        <  2:1562485/93‑1 (MQ=255)

                                                                 ggggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                        >  2:1539073/1‑93 (MQ=255)

                                                                 ggggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                        >  2:720766/1‑93 (MQ=255)

                                                                 ggggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAg                        <  1:2009313/93‑1 (MQ=255)

                                                                  gggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGc                       <  1:2724940/93‑1 (MQ=255)

                                                                  gggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGc                       <  2:3239103/93‑1 (MQ=255)

                                                                   gtttttttGCATGCACAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCg                      <  1:2098555/91‑1 (MQ=255)

                                                                   ggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCg                      <  1:6240194/93‑1 (MQ=255)

                                                                   ggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCg                      >  2:5084931/1‑93 (MQ=255)

                                                                   ggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCg                      <  2:5642757/93‑1 (MQ=255)

                                                                    gTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCgg                     <  1:251167/93‑1 (MQ=255)

                                                                     ttttttGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGt                    <  1:49223/93‑1 (MQ=255)

                                                                       ttttGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGttt                  <  1:5365759/93‑1 (MQ=255)

                                                                       ttttGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGttt                  >  2:58761/1‑93 (MQ=255)

                                                                       ttttGCATGCGCAACCCAAGGGCCACATTATCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGttt                  <  2:5901635/93‑1 (MQ=255)

                                                                          tGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTcc               <  1:2220094/93‑1 (MQ=255)

                                                                           gCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccg              <  2:2029217/93‑1 (MQ=255)

                                                                            cATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgc             <  2:6605544/93‑1 (MQ=255)

                                                                             aTGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc            >  2:775241/1‑93 (MQ=255)

                                                                             aTGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGCAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc            >  2:1251101/1‑93 (MQ=255)

                                                                              tGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgccg           >  2:5231837/1‑93 (MQ=255)

                                                                              tGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgccg           <  2:1672118/93‑1 (MQ=255)

                                                                                 gcAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGaa        <  2:5812160/93‑1 (MQ=255)

                                                                                  cAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAAc       <  2:4255535/93‑1 (MQ=255)

                                                                                  cAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAAc       <  1:2952496/93‑1 (MQ=255)

                                                                                   aaCCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACg      >  2:3911876/1‑93 (MQ=255)

                                                                                    aCCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGt     <  2:4109276/93‑1 (MQ=255)

                                                                                     cccAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTg    <  1:2560786/93‑1 (MQ=255)

                                                                                     cccAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTg    >  2:3454679/1‑93 (MQ=255)

                                                                                      ccAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTgg   >  1:461940/1‑93 (MQ=255)

                                                                                       cAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg  <  1:3725694/93‑1 (MQ=255)

GAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGG  >  NC_000913/1184394‑1184573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`