BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,289,543	T→C	F27L (TTT→CTT)	hnr →	Hnr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,289,543	0	T	C	99.3%	872.1 / ‑3.8	270	F27L (TTT→CTT)	hnr	Hnr
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (147/121); total (148/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 285 total aligned reads displayed.

AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATA  >  NC_000913/1289453‑1289632

aGGGGAGAGAACATGCCGCAGCCATTGGTCGGAAAGCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGctt                                                                                         <  1:6350374/93‑1 (MQ=255)

aGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGctt                                                                                         <  1:5799614/93‑1 (MQ=255)

 ggggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGcttt                                                                                        >  1:6289666/1‑93 (MQ=255)

 ggggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGcttt                                                                                        >  1:204877/1‑93 (MQ=255)

  gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTc                                                                                       >  2:2164501/1‑93 (MQ=255)

    gagagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCt                                                                                     <  1:3375682/93‑1 (MQ=255)

    gagagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCt                                                                                     <  2:5044168/93‑1 (MQ=255)

    gagagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCt                                                                                     >  2:2570423/1‑93 (MQ=255)

    gagagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCt                                                                                     <  1:1610069/93‑1 (MQ=255)

     agagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTc                                                                                    <  2:4600106/93‑1 (MQ=255)

       agaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTAAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCAt                                                                                  <  1:867687/93‑1 (MQ=255)

         aaCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTg                                                                                <  2:76758/93‑1 (MQ=255)

         aaCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTg                                                                                >  1:6683251/1‑93 (MQ=255)

          aCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTgg                                                                               >  1:528596/1‑93 (MQ=255)

          aCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTgg                                                                               <  2:376544/93‑1 (MQ=255)

           cATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTggg                                                                              <  1:2435212/93‑1 (MQ=255)

           cATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTggg                                                                              >  2:3656615/1‑93 (MQ=255)

               aCGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGGTTCATGGCTTTCCTCATTGGGAGCg                                                                          >  1:585231/1‑93 (MQ=255)

               aCGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCg                                                                          >  1:3168489/1‑93 (MQ=255)

                 gCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGTAGCGAc                                                                        >  1:1452794/1‑93 (MQ=255)

                   aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACac                                                                      >  2:477695/1‑92 (MQ=255)

                      cATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACgg                                                                   >  1:1989562/1‑93 (MQ=255)

                       aTTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGt                                                                  >  1:6540012/1‑93 (MQ=255)

                       aTTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGt                                                                  >  2:414443/1‑93 (MQ=255)

                       aTTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGt                                                                  <  1:1816112/93‑1 (MQ=255)

                        ttGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTa                                                                 >  1:3437827/1‑93 (MQ=255)

                        ttGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTa                                                                 >  1:1723951/1‑93 (MQ=255)

                         tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTAc                                                                >  1:1265999/1‑93 (MQ=255)

                         tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTAc                                                                <  2:679063/93‑1 (MQ=255)

                          ggTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACt                                                               <  2:914420/93‑1 (MQ=255)

                          ggTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACt                                                               <  2:2093156/93‑1 (MQ=255)

                               gAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcgg                                                          <  1:4360382/93‑1 (MQ=255)

                                aaaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcggc                                                         >  1:4769235/1‑93 (MQ=255)

                                 aaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcggc                                                         <  2:5328362/92‑1 (MQ=255)

                                 aaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcggc                                                         >  1:5328362/1‑92 (MQ=255)

                                 aaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt                                                        <  2:1276948/93‑1 (MQ=255)

                                 aaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt                                                        >  1:4490166/1‑93 (MQ=255)

                                  aaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTg                                                       >  1:6128911/1‑93 (MQ=255)

                                   aCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGa                                                      <  2:5895848/93‑1 (MQ=255)

                                    cAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAt                                                     <  1:5045612/93‑1 (MQ=255)

                                      gATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg                                                   >  2:5554228/1‑93 (MQ=255)

                                      gATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg                                                   >  2:2675204/1‑93 (MQ=255)

                                        ttCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg                                                 >  2:5774089/1‑93 (MQ=255)

                                        ttCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg                                                 <  1:6436432/93‑1 (MQ=255)

                                        ttCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg                                                 <  2:5671979/93‑1 (MQ=255)

                                        ttCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg                                                 <  2:3313917/93‑1 (MQ=255)

                                         tctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTGCTGGCGGCTGATGGGGt                                                <  1:6225642/93‑1 (MQ=255)

                                          ctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTg                                               >  2:5989313/1‑93 (MQ=255)

                                          ctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTg                                               <  1:1181390/93‑1 (MQ=255)

                                           tcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCACGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTgg                                              <  2:4479705/93‑1 (MQ=255)

                                            cATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGa                                             <  1:5130699/93‑1 (MQ=255)

                                                gttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGGTGcc                                         >  1:2751084/1‑93 (MQ=255)

                                                gttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGcc                                         >  2:1780197/1‑93 (MQ=255)

                                                gttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGcc                                         >  2:2651982/1‑93 (MQ=255)

                                                      gATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAg                                   >  2:1065319/1‑93 (MQ=255)

                                                      gATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAg                                   >  2:3354731/1‑93 (MQ=255)

                                                       aTGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGt                                  >  1:2691129/1‑93 (MQ=255)

                                                         gAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTg                                >  2:2422962/1‑93 (MQ=255)

                                                          aGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGc                               <  2:3608326/93‑1 (MQ=255)

                                                          aGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGc                               >  2:1660657/1‑93 (MQ=255)

                                                            cAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTg                             <  2:3880/93‑1 (MQ=255)

                                                             aGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgg                            <  2:1825264/93‑1 (MQ=255)

                                                             aGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgg                            >  2:6104539/1‑93 (MQ=255)

                                                              ggTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTggg                           >  1:6656476/1‑93 (MQ=255)

                                                               gTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGa                          <  2:5255584/93‑1 (MQ=255)

                                                               gTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGa                          >  2:806024/1‑93 (MQ=255)

                                                                tATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAg                         <  2:1946227/93‑1 (MQ=255)

                                                                 aTTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTTCTGGGAgg                        >  1:1006712/1‑93 (MQ=255)

                                                                 aTTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAgg                        >  2:3093484/1‑93 (MQ=255)

                                                                 aTTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAgg                        >  2:5414513/1‑93 (MQ=255)

                                                                 aTTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGAGGATGCCCTTGAGTTGCTgggggg                        >  1:2634466/1‑93 (MQ=255)

                                                                  tttCTCTCGCTTCTGGATTGATGGCTTTCCTCATTGGGAGCTACAACGGTACGGGCGGCTGATGGGGTGGATGCTCTTGAGTTTCTGGGAGGt                       <  1:1639328/93‑1 (MQ=255)

                                                                  tttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGt                       <  1:2281496/93‑1 (MQ=255)

                                                                  tttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGt                       >  2:4097966/1‑93 (MQ=255)

                                                                    tcgctcgcTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGgtt                     >  2:1201701/1‑93 (MQ=255)

                                                                     cgctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTc                    <  2:1143390/93‑1 (MQ=255)

                                                                       ctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAc                  <  1:5392939/93‑1 (MQ=255)

                                                                       ctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAc                  <  2:2640215/93‑1 (MQ=255)

                                                                       ctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCAc                  >  1:709562/1‑93 (MQ=255)

                                                                        tcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACt                 <  1:4192181/93‑1 (MQ=255)

                                                                        tcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACt                 <  1:620005/93‑1 (MQ=255)

                                                                           ctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCa              <  2:3728401/93‑1 (MQ=255)

                                                                           ctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCACTCCa              >  1:3794077/1‑93 (MQ=255)

                                                                            ttCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAg             >  1:4295494/1‑93 (MQ=255)

                                                                            ttCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAg             >  1:5598504/1‑93 (MQ=255)

                                                                              cTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGAc           >  1:3327894/1‑93 (MQ=255)

                                                                               tGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGAcc          >  2:3017489/1‑93 (MQ=255)

                                                                               tGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGAcc          >  2:6396842/1‑93 (MQ=255)

                                                                                ggATTCATGGCTTTCCTCATTGGGAGCGACAGCGGTACTGGCGGCTGATGGGGTGGATGCCCTTCAGTTGCTGGGAGGTTTCACTCCAGACCt         <  1:924746/93‑1 (MQ=255)

                                                                                ggATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCt         >  2:4372674/1‑93 (MQ=255)

                                                                                  aTTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTAACTCCAGACCtga       >  1:4070444/1‑93 (MQ=255)

                                                                                   ttCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgat      >  1:284671/1‑93 (MQ=255)

                                                                                   ttCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgat      >  2:2850200/1‑93 (MQ=255)

                                                                                   ttCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgat      <  2:2294761/93‑1 (MQ=255)

                                                                                   ttCATGGCTTTCCTCATTGGGAGCGACAACGGTACCGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgat      >  2:1380727/1‑93 (MQ=255)

                                                                                    tCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCACTCCAGACCtgatg     >  1:2994569/1‑93 (MQ=255)

                                                                                     cATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgatga    <  1:3665358/93‑1 (MQ=255)

                                                                                     cATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgatga    <  1:604691/93‑1 (MQ=255)

                                                                                       tGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACTTGATGata  <  2:6114130/93‑1 (MQ=255)

                                                                                       tGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGata  <  1:1378742/93‑1 (MQ=255)

AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATA  >  NC_000913/1289453‑1289632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`