BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,303,961	A→G	E58E (GAA→GAG)	oppF →	OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,303,961	0	A	G	100.0%	767.1 / NA	238	E58E (GAA→GAG)	oppF	OppF
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (128/110); total (128/110)

Only 100 of 246 total aligned reads displayed.

GTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGT  >  NC_000913/1303871‑1304049

gTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTc                                                                                        >  1:4236269/1‑93 (MQ=255)

gTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTc                                                                                        >  1:4585727/1‑93 (MQ=255)

  ggTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCgg                                                                                      >  1:6221218/1‑93 (MQ=255)

   gtgcTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCggg                                                                                     <  2:5480165/90‑1 (MQ=255)

    ttCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGa                                                                                    <  1:5533277/93‑1 (MQ=255)

    ttCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGa                                                                                    >  1:60035/1‑93 (MQ=255)

    ttCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGa                                                                                    <  2:4552948/93‑1 (MQ=255)

       tGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATgg                                                                                 >  1:1337296/1‑92 (MQ=255)

        ggCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCg                                                                                >  2:2164593/1‑93 (MQ=255)

        ggCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCg                                                                                <  2:4749732/93‑1 (MQ=255)

        ggCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCg                                                                                >  1:4323067/1‑93 (MQ=255)

         gCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCgg                                                                               <  2:3927807/93‑1 (MQ=255)

          cAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGt                                                                              >  2:2405056/1‑93 (MQ=255)

           acccgccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGTTAGGGGAGTCGGGATGCGGTa                                                                             <  1:3683529/91‑1 (MQ=255)

            aCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTaa                                                                            <  2:3599258/93‑1 (MQ=255)

             ccgccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAg                                                                           >  1:3023728/1‑93 (MQ=255)

               gccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTc                                                                         >  1:6169829/1‑93 (MQ=255)

                ccgAAAACGCTCAAAGCCGTCGCTGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTcc                                                                        <  2:6511197/93‑1 (MQ=255)

                ccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTcc                                                                        >  1:1601035/1‑93 (MQ=255)

                 cgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCa                                                                       <  2:1218956/93‑1 (MQ=255)

                   aaaaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCAcc                                                                     >  1:2758320/1‑93 (MQ=255)

                    aaaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCt                                                                    <  1:898905/93‑1 (MQ=255)

                     aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCtt                                                                   <  1:5682348/93‑1 (MQ=255)

                     aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCtt                                                                   >  1:4870011/1‑93 (MQ=255)

                     aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCtt                                                                   <  1:2053419/93‑1 (MQ=255)

                     aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCtt                                                                   >  1:975660/1‑93 (MQ=255)

                     aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCtt                                                                   <  2:2866356/93‑1 (MQ=255)

                      aCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCttt                                                                  >  1:1023932/1‑93 (MQ=255)

                          tCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTc                                                              >  2:5940097/1‑93 (MQ=255)

                            aaaGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTcgc                                                            >  1:6633057/1‑93 (MQ=255)

                               gCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGcc                                                         >  2:3763108/1‑93 (MQ=255)

                                 cGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcat                                                       <  2:4144917/93‑1 (MQ=255)

                                  gTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatc                                                      <  2:1477446/93‑1 (MQ=255)

                                  gTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatc                                                      <  2:4949528/93‑1 (MQ=255)

                                  gTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatc                                                      >  1:610570/1‑93 (MQ=255)

                                        ggTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGt                                                <  2:4240623/93‑1 (MQ=255)

                                            tCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTgg                                            <  2:420805/93‑1 (MQ=255)

                                            tCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTgg                                            <  1:5298979/93‑1 (MQ=255)

                                            tCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTgg                                            >  1:5431794/1‑93 (MQ=255)

                                            tCACTCTTCGCCTGTATGAAGGGGAAACATTAGGCGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTgg                                            >  1:2004117/1‑93 (MQ=255)

                                             cACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGt                                           >  2:2690970/1‑93 (MQ=255)

                                             cACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGt                                           <  2:3455750/93‑1 (MQ=255)

                                             cACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGt                                           >  1:30822/1‑93 (MQ=255)

                                              cctctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTc                                          <  1:1461901/92‑1 (MQ=255)

                                              aCTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTc                                          >  1:2301663/1‑93 (MQ=255)

                                              aCTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTc                                          >  1:1719232/1‑93 (MQ=255)

                                              aCTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTc                                          >  2:2801179/1‑93 (MQ=255)

                                              aCTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCACGCGCCATCATCGGTTTGGTc                                          <  2:6667483/93‑1 (MQ=255)

                                              aCTCTTCGCCTGTATGAAGGGGAAACATTAGGAGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTGTGGta                                          >  1:3385527/1‑92 (MQ=255)

                                               ctctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCa                                         >  2:1828999/1‑93 (MQ=255)

                                               ctctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCa                                         <  1:4704725/93‑1 (MQ=255)

                                                tctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCag                                        >  2:5275405/1‑92 (MQ=255)

                                                 ctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAg                                       >  2:5885575/1‑93 (MQ=255)

                                                 ctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAg                                       >  2:1807767/1‑93 (MQ=255)

                                                 ctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAg                                       <  1:1223823/93‑1 (MQ=255)

                                                  ttCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAgg                                      >  2:4460334/1‑93 (MQ=255)

                                                  ttCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAgg                                      >  2:4581199/1‑93 (MQ=255)

                                                   tCGCCTGTCTGAAGGGTAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCCCCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGc                                     <  2:2915403/93‑1 (MQ=255)

                                                     gCCTGTATGAAGGGGAAACATTAGGTGTGGTGGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcga                                   <  1:6477693/93‑1 (MQ=255)

                                                      ccTGTATTAAGGGGAAACATTAGGTGTGGTAGGGGAGTTGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                  <  2:827401/93‑1 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCCCCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                  <  2:2476345/93‑1 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                  <  2:5020857/93‑1 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                  <  1:193773/93‑1 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                  >  2:4844907/1‑93 (MQ=255)

                                                       cTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgacc                                 >  1:185434/1‑93 (MQ=255)

                                                            tGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCGCCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACgg                            <  1:4063385/93‑1 (MQ=255)

                                                             gAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAATGCGACCGACGGt                           >  2:4328138/1‑93 (MQ=255)

                                                              aaGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTc                          >  2:6734320/1‑93 (MQ=255)

                                                              aaGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTc                          >  1:5759534/1‑93 (MQ=255)

                                                                ggggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTTAt                        >  1:1484116/1‑93 (MQ=255)

                                                                ggggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCAt                        >  1:2371120/1‑93 (MQ=255)

                                                                ggggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCAt                        >  1:4776458/1‑93 (MQ=255)

                                                                 gggAAACATTAGGTGTGGTAGGGGCGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATg                       >  1:1115232/1‑93 (MQ=255)

                                                                  ggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGt                      <  2:651816/93‑1 (MQ=255)

                                                                  ggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGt                      <  1:6204046/93‑1 (MQ=255)

                                                                  ggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGt                      >  1:97999/1‑93 (MQ=255)

                                                                  ggAAACATTAGGGGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGt                      <  1:2424862/93‑1 (MQ=255)

                                                                   gAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGtt                     >  2:3198610/1‑93 (MQ=255)

                                                                    aaaCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTg                    <  2:6581389/93‑1 (MQ=255)

                                                                    aaaCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTg                    >  1:3333640/1‑93 (MQ=255)

                                                                      aCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                  <  1:4609248/93‑1 (MQ=255)

                                                                      aCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                  >  1:6236976/1‑93 (MQ=255)

                                                                      aCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                  <  2:1995482/93‑1 (MQ=255)

                                                                      aCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                  >  2:1568060/1‑93 (MQ=255)

                                                                       cATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCt                 >  2:6165222/1‑93 (MQ=255)

                                                                       cATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCt                 <  2:2504634/93‑1 (MQ=255)

                                                                        aTTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTg                >  2:6067627/1‑93 (MQ=255)

                                                                        aTTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTg                >  2:5320343/1‑93 (MQ=255)

                                                                         ttAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGGCCACCTTTGCTCGCGCCATCATCGGGTTGGTCAACGCGACCCACGGTCATGTTGCCttg               >  1:3547004/1‑91 (MQ=255)

                                                                           aGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGtt             >  1:1770323/1‑93 (MQ=255)

                                                                             gtgtGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAg           <  1:281540/93‑1 (MQ=255)

                                                                              tgtgGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAgg          <  2:4302536/93‑1 (MQ=255)

                                                                                tgGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTa        <  2:3378327/93‑1 (MQ=255)

                                                                                tgGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGGCATGCTGCCTGGTTAGGTa        >  1:797150/1‑93 (MQ=255)

                                                                                   tAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAag     <  2:1695541/93‑1 (MQ=255)

                                                                                    aGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAaga    <  2:511403/93‑1 (MQ=255)

                                                                                     ggggAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAagag   >  1:3478140/1‑93 (MQ=255)

                                                                                     ggggAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAagag   >  1:3823414/1‑93 (MQ=255)

                                                                                     ggggAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAagag   >  1:4950197/1‑93 (MQ=255)

                                                                                     ggggAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAagag   >  2:1433488/1‑93 (MQ=255)

GTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGT  >  NC_000913/1303871‑1304049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`