BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	122,235	T→C	R48R (CGT→CGC)	pdhR →	transcriptional regulator for pyruvate dehydrogenase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	122,235	0	T	C	100.0%	786.0 / NA	244	R48R (CGT→CGC)	pdhR	transcriptional regulator for pyruvate dehydrogenase complex
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (128/116); total (128/116)

Only 100 of 261 total aligned reads displayed.

ACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGTCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCT  >  NC_000913/122145‑122324

aCTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGccc                                                                                         >  1:3571343/1‑93 (MQ=255)

aCTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGccc                                                                                         <  1:323672/93‑1 (MQ=255)

 cTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGcccc                                                                                        >  1:4544068/1‑93 (MQ=255)

  tGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCcct                                                                                       >  1:3135576/1‑93 (MQ=255)

   ggAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCcctc                                                                                      >  1:4672297/1‑93 (MQ=255)

    gAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCcctcc                                                                                     >  2:2531828/1‑93 (MQ=255)

     aGTTTTTGATCCTCGAAGGCCCTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCcctcct                                                                                    <  1:397624/93‑1 (MQ=255)

     aGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCcctcct                                                                                    <  2:1467986/93‑1 (MQ=255)

      gTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCtt                                                                                   >  1:1597253/1‑93 (MQ=255)

      gTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCtt                                                                                   <  2:1735511/93‑1 (MQ=255)

      gTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCACTCCtt                                                                                   >  1:1020867/1‑93 (MQ=255)

       tttttGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTg                                                                                  <  1:5089144/93‑1 (MQ=255)

       tttttGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTg                                                                                  <  1:928863/93‑1 (MQ=255)

        ttttGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGc                                                                                 >  1:3033142/1‑93 (MQ=255)

          ttGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGt                                                                               <  1:6045867/93‑1 (MQ=255)

          ttGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGt                                                                               >  2:5456575/1‑93 (MQ=255)

             aTCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAg                                                                            >  2:4273862/1‑93 (MQ=255)

             aTCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAAAAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAg                                                                            >  1:793605/1‑93 (MQ=255)

              tCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAgg                                                                           >  2:6351867/1‑93 (MQ=255)

              tCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAgg                                                                           >  2:624421/1‑93 (MQ=255)

              cccTCGAAGGCACTCTCCGCCCGGGCGAAAAGCGCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAgg                                                                           <  1:5636462/92‑1 (MQ=255)

               ccTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGGTTGAC‑GTCTCCCGCCCCTCCTTTCGTGAggg                                                                          >  1:4265978/1‑92 (MQ=255)

                    aaGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTc                                                                     >  2:5678610/1‑93 (MQ=255)

                     aGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCa                                                                    >  2:4870873/1‑93 (MQ=255)

                      ggCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCaa                                                                   >  2:4968193/1‑93 (MQ=255)

                       gCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAAc                                                                  >  2:5504313/1‑93 (MQ=255)

                        ccctctCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACg                                                                 <  2:2027436/91‑1 (MQ=255)

                        cACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACg                                                                 >  2:5809662/1‑93 (MQ=255)

                          ctctCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtc                                                               >  2:1179942/1‑93 (MQ=255)

                           tctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtct                                                              >  1:2539749/1‑93 (MQ=255)

                            ctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtctc                                                             <  2:6709682/93‑1 (MQ=255)

                             tcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcg                                                            >  2:351942/1‑93 (MQ=255)

                               cGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaa                                                          <  1:4016728/93‑1 (MQ=255)

                               cGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaa                                                          >  1:5778238/1‑93 (MQ=255)

                                 cccGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagc                                                        <  2:1268225/93‑1 (MQ=255)

                                 cccGGGCGAAAAACCCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagc                                                        <  1:1150197/93‑1 (MQ=255)

                                   cGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcga                                                      >  2:3169885/1‑93 (MQ=255)

                                    gggCGAAAACCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcgaa                                                     <  1:3060611/93‑1 (MQ=255)

                                     ggCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcgaag                                                    <  1:434582/93‑1 (MQ=255)

                                        gAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGc                                                 >  1:472430/1‑93 (MQ=255)

                                         aaaaaCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCt                                                <  2:2708953/93‑1 (MQ=255)

                                         aaaaaCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCt                                                <  2:5296055/93‑1 (MQ=255)

                                           aaaCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttg                                              >  1:6166561/1‑93 (MQ=255)

                                             aCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttgtt                                            >  2:1148728/1‑93 (MQ=255)

                                             aCTCCCACCGGAACGCGAACTGGCAAAACAGGTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttgtg                                            >  1:1936591/1‑92 (MQ=255)

                                               tCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGc                                          >  1:5122974/1‑93 (MQ=255)

                                                cccACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCt                                         <  1:2223798/93‑1 (MQ=255)

                                                cccACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCt                                         <  1:4136983/93‑1 (MQ=255)

                                                 ccACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCtt                                        <  2:3933994/93‑1 (MQ=255)

                                                  cACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtc                                       >  1:1851943/1‑93 (MQ=255)

                                                  cACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtc                                       >  2:5108582/1‑93 (MQ=255)

                                                   acgggAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcg                                      <  1:5895845/90‑1 (MQ=255)

                                                   aCCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcg                                      >  1:4113210/1‑93 (MQ=255)

                                                    ccGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcgt                                     >  2:5499254/1‑93 (MQ=255)

                                                         aCGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCa                                >  2:3062957/1‑93 (MQ=255)

                                                         aCGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCa                                >  1:2592552/1‑93 (MQ=255)

                                                         aCGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCa                                >  1:1745881/1‑93 (MQ=255)

                                                           gcgAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAgg                              <  1:3805601/93‑1 (MQ=255)

                                                           gcgAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAgg                              >  2:3403132/1‑93 (MQ=255)

                                                            cgAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAggg                             >  2:1950378/1‑93 (MQ=255)

                                                            cgAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAgga                             >  2:6431622/1‑92 (MQ=255)

                                                             gAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGt                            <  2:2274223/93‑1 (MQ=255)

                                                             gAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGt                            <  2:2870750/93‑1 (MQ=255)

                                                             gAACTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCCTCGTCGCCAggcg                            >  1:550759/1‑91 (MQ=255)

                                                               aCTGGCAAAACAGTTTGAC‑GTCTCCGGCCCCTCCTTGCGCGAGGCGATTCAACGTCTCGAAGCGATGCGC‑TGGTGCTTCCTCGCCAAGggggg                         >  2:2940088/1‑92 (MQ=255)

                                                               aCTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTgg                          <  2:6263323/93‑1 (MQ=255)

                                                               aCTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTgg                          >  2:942048/1‑93 (MQ=255)

                                                                cTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggc                         <  1:6632514/93‑1 (MQ=255)

                                                                cTGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggc                         <  1:2384456/93‑1 (MQ=255)

                                                                atGGGAAAACACTTTTACGGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTgg                          <  2:452873/92‑1 (MQ=255)

                                                                 tGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcg                        <  2:3769801/93‑1 (MQ=255)

                                                                 tGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcg                        <  2:2136600/93‑1 (MQ=255)

                                                                 tGGCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcg                        <  2:4102326/93‑1 (MQ=255)

                                                                 cggCAAAACAGTTTGAC‑GCCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcg                        <  1:6336777/92‑1 (MQ=255)

                                                                  ggCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcgg                       >  1:3312572/1‑93 (MQ=255)

                                                                   gCAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcggc                      >  2:2218485/1‑93 (MQ=255)

                                                                    cAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCa                     <  1:6576076/93‑1 (MQ=255)

                                                                    cAAAACAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCa                     >  1:2920356/1‑93 (MQ=255)

                                                                     aaaaCAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCAc                    >  2:4177460/1‑93 (MQ=255)

                                                                      aaaCAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTTAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACt                   >  2:3562358/1‑93 (MQ=255)

                                                                      aaaCAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACt                   >  1:5185369/1‑93 (MQ=255)

                                                                      aaaCAGTTTGAC‑GTCGCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACt                   <  2:4671713/93‑1 (MQ=255)

                                                                       aaCAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACtt                  <  1:3669789/93‑1 (MQ=255)

                                                                        aCAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACttt                 <  1:1289318/93‑1 (MQ=255)

                                                                        aCAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACttt                 >  2:6393330/1‑93 (MQ=255)

                                                                        aCAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACttt                 <  1:5499254/93‑1 (MQ=255)

                                                                        aCAGTTAGAC‑GTCTCCCGCCCCTCCTAGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACttt                 <  2:4665939/93‑1 (MQ=255)

                                                                         cAGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGGGGCGGCACtttt                >  1:3716145/1‑93 (MQ=255)

                                                                          aGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTg               <  2:3138181/93‑1 (MQ=255)

                                                                          aGTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTg               <  2:5574872/93‑1 (MQ=255)

                                                                           gTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGt              >  2:2459141/1‑93 (MQ=255)

                                                                           gTTTGAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGt              >  1:4887873/1‑93 (MQ=255)

                                                                             tggAC‑GGCCCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTcc            <  2:728079/91‑1 (MQ=255)

                                                                               gAC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCag          >  1:742355/1‑93 (MQ=255)

                                                                                aC‑GTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCaga         >  1:5064205/1‑93 (MQ=255)

                                                                                      tcCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGagcagc    >  1:5381426/1‑93 (MQ=255)

                                                                                       cccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGcc   <  1:1385232/93‑1 (MQ=255)

                                                                                        ccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCt  <  2:3681341/93‑1 (MQ=255)

                                                                                        ccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCt  >  2:5399414/1‑93 (MQ=255)

                                                                                        ccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCt  >  1:2728499/1‑93 (MQ=255)

ACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGAC‑GTCTCCCGTCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCT  >  NC_000913/122145‑122324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`