BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,347,664	A→G	T182T (ACT→ACC)	yciW ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,347,664	0	A	G	99.6%	838.8 / ‑4.9	256	T182T (ACT→ACC)	yciW	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (135/120); total (135/121)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.70e-01

Only 100 of 261 total aligned reads displayed.

ATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTG  >  NC_000913/1347574‑1347753

aTGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGt                                                                                         >  2:6551235/1‑93 (MQ=255)

aTGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGt                                                                                         >  2:339043/1‑93 (MQ=255)

  gCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTaa                                                                                       <  1:5257259/93‑1 (MQ=255)

     cGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGAc                                                                                    <  1:1910238/93‑1 (MQ=255)

      gagaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGAcc                                                                                   <  2:1569317/93‑1 (MQ=255)

      gagaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGAcc                                                                                   >  2:450131/1‑93 (MQ=255)

       agaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCg                                                                                  <  1:5420500/93‑1 (MQ=255)

        gaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGa                                                                                 >  2:1336157/1‑93 (MQ=255)

        gaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGa                                                                                 >  2:6504558/1‑93 (MQ=255)

          tAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACg                                                                               >  2:397631/1‑93 (MQ=255)

            aGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTc                                                                             >  1:4666381/1‑93 (MQ=255)

            aGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTc                                                                             <  1:6619234/93‑1 (MQ=255)

            aGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTc                                                                             >  2:3497521/1‑93 (MQ=255)

                tGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTc                                                                         <  2:4798261/93‑1 (MQ=255)

                 gAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCa                                                                        <  1:2692056/93‑1 (MQ=255)

                 gAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCa                                                                        <  1:3069439/93‑1 (MQ=255)

                  aaaTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAg                                                                       >  2:3004516/1‑93 (MQ=255)

                  aaaTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAg                                                                       <  2:4632523/93‑1 (MQ=255)

                   aaTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGa                                                                      >  1:1073488/1‑93 (MQ=255)

                   aaTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGa                                                                      <  2:5557745/93‑1 (MQ=255)

                    aTGTGGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGAt                                                                     >  1:3035683/1‑93 (MQ=255)

                    aTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGAt                                                                     >  2:4568383/1‑93 (MQ=255)

                    aTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGAt                                                                     <  2:5698165/93‑1 (MQ=255)

                        cGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTg                                                                 >  1:4815033/1‑93 (MQ=255)

                         gCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTgg                                                                <  2:2185221/93‑1 (MQ=255)

                         gCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTgg                                                                <  1:1669171/93‑1 (MQ=255)

                           aaTTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGgcg                                                              >  2:2896753/1‑93 (MQ=255)

                           aaTTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGgcg                                                              >  1:2572607/1‑93 (MQ=255)

                            aTTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGgcgc                                                             >  1:570721/1‑93 (MQ=255)

                            aTTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGgcgc                                                             >  2:5403108/1‑93 (MQ=255)

                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         <  2:1744603/93‑1 (MQ=255)

                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         <  1:6180879/93‑1 (MQ=255)

                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         >  1:607391/1‑93 (MQ=255)

                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         <  1:2726995/93‑1 (MQ=255)

                                  cgcgCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCgg                                                       >  2:5485549/1‑93 (MQ=255)

                                   gcgcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGt                                                      >  1:4524420/1‑93 (MQ=255)

                                   gcgcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGt                                                      <  1:814291/93‑1 (MQ=255)

                                    cgcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGtt                                                     >  2:5059880/1‑93 (MQ=255)

                                    cgcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGtt                                                     <  2:1260163/93‑1 (MQ=255)

                                     gcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGGTg                                                    >  2:4411196/1‑93 (MQ=255)

                                       cTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTc                                                  <  2:3571866/93‑1 (MQ=255)

                                        tGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCg                                                 <  2:4382463/93‑1 (MQ=255)

                                        tGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCg                                                 >  2:2491643/1‑93 (MQ=255)

                                        tGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCg                                                 >  1:3458494/1‑93 (MQ=255)

                                        cgAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCg                                                 <  1:1246740/92‑1 (MQ=255)

                                         gAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGa                                                >  1:1772467/1‑93 (MQ=255)

                                          aaaGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGat                                               <  2:2804368/93‑1 (MQ=255)

                                          aaaGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGGCATTCAAATGCTGGCGCGATCGGGTGTCggt                                               >  1:5016134/1‑91 (MQ=255)

                                           aaGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGata                                              <  1:2278520/93‑1 (MQ=255)

                                            aGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataa                                             <  1:5954456/93‑1 (MQ=255)

                                            aGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataa                                             <  2:59839/93‑1 (MQ=255)

                                            aGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataa                                             <  2:6020935/93‑1 (MQ=255)

                                             gCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGatact                                            >  2:4967757/1‑91 (MQ=255)

                                             gCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataat                                            >  2:6239478/1‑93 (MQ=255)

                                              ccAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataata                                           <  2:6332891/93‑1 (MQ=255)

                                              ccAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataata                                           <  1:4749083/93‑1 (MQ=255)

                                              ccAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataata                                           <  1:4035254/93‑1 (MQ=255)

                                               cAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAg                                          <  1:3509939/93‑1 (MQ=255)

                                                 aTAAAACCAATGATTTGGCTAATGATCACACAGTCCTAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGaa                                        <  1:5772956/93‑1 (MQ=255)

                                                 aTAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGaa                                        >  1:3905966/1‑93 (MQ=255)

                                                 aTAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGaa                                        <  2:5145091/93‑1 (MQ=255)

                                                 aTAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGaa                                        >  1:505786/1‑93 (MQ=255)

                                                  tAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAg                                       <  1:6364426/93‑1 (MQ=255)

                                                   aaaaCCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAgg                                      >  1:2385619/1‑93 (MQ=255)

                                                    aaaCCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGc                                     <  1:1633304/93‑1 (MQ=255)

                                                     aaCCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCg                                    <  1:2233781/93‑1 (MQ=255)

                                                      aCCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGa                                   <  1:4580839/93‑1 (MQ=255)

                                                      aCCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGa                                   >  1:5496486/1‑93 (MQ=255)

                                                         aaTGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCt                                >  1:6259258/1‑93 (MQ=255)

                                                          aTGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTa                               <  2:181803/93‑1 (MQ=255)

                                                            gATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGc                             >  2:416431/1‑93 (MQ=255)

                                                             aTTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGcc                            >  1:5322238/1‑93 (MQ=255)

                                                                 ggCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGtt                        >  2:4842300/1‑93 (MQ=255)

                                                                    tAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGc                     >  1:2791218/1‑93 (MQ=255)

                                                                    tAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGc                     >  1:4720283/1‑93 (MQ=255)

                                                                    tAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGc                     >  1:3841789/1‑93 (MQ=255)

                                                                    tAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGc                     <  2:5883977/93‑1 (MQ=255)

                                                                      aTGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCgg                   >  1:320173/1‑93 (MQ=255)

                                                                       tGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCggg                  >  1:6477345/1‑93 (MQ=255)

                                                                          tCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCat               >  2:4251266/1‑93 (MQ=255)

                                                                            acacaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCatat             >  1:4278880/1‑93 (MQ=255)

                                                                            acacaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGACAGTTCGCGGGAatat             >  2:853361/1‑93 (MQ=255)

                                                                              acaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTg           >  1:1883226/1‑93 (MQ=255)

                                                                               caGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGc          >  1:2831016/1‑93 (MQ=255)

                                                                               caGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGc          <  2:5774855/93‑1 (MQ=255)

                                                                                aGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCg         <  2:5283569/93‑1 (MQ=255)

                                                                                aGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCg         >  1:1410617/1‑93 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  2:5820578/1‑93 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  2:644986/1‑93 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        <  1:6706248/93‑1 (MQ=255)

                                                                                    ccAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAt     <  2:1460561/93‑1 (MQ=255)

                                                                                    ccAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAt     >  1:4014416/1‑93 (MQ=255)

                                                                                     cAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAtt    >  2:2783096/1‑93 (MQ=255)

                                                                                     cAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAtt    >  2:194942/1‑93 (MQ=255)

                                                                                     cAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAtt    <  1:4076294/93‑1 (MQ=255)

                                                                                      aGGCGGTAGGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAttt   <  1:4403946/93‑1 (MQ=255)

                                                                                       ggCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTtg  >  2:2074634/1‑93 (MQ=255)

                                                                                       ggCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTtg  <  1:743335/93‑1 (MQ=255)

                                                                                       ggCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTtg  >  1:114103/1‑93 (MQ=255)

                                                                                       ggCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGGTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTtg  >  2:1055181/1‑93 (MQ=255)

ATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTG  >  NC_000913/1347574‑1347753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`