BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,456,531	A→G	M82V (ATG→GTG)	b1394 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,456,531	0	A	G	99.5%	628.2 / NA	193	M82V (ATG→GTG)	b1394	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (93/99); minor base T (0/1); total (93/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

TTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCA  >  NC_000913/1456441‑1456620

ttacttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTg                                                                                         >  2:4278021/1‑93 (MQ=255)

ttacttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTg                                                                                         <  1:341834/93‑1 (MQ=255)

  acttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTc                                                                                       <  1:823626/93‑1 (MQ=255)

  acttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTc                                                                                       <  2:1686883/93‑1 (MQ=255)

  acttacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTc                                                                                       <  2:1779970/93‑1 (MQ=255)

     tacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGt                                                                                    <  1:5941646/93‑1 (MQ=255)

     tacAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGt                                                                                    <  2:3364978/93‑1 (MQ=255)

      acAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGtt                                                                                   <  1:3353351/93‑1 (MQ=255)

        aGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGa                                                                                 >  2:4991562/1‑93 (MQ=255)

         ggTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGaa                                                                                >  2:2746857/1‑93 (MQ=255)

             ccGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGtt                                                                            <  1:1967340/93‑1 (MQ=255)

              cGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGttt                                                                           >  1:5467570/1‑93 (MQ=255)

              cGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGttt                                                                           <  1:676782/93‑1 (MQ=255)

               gggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTc                                                                          >  1:1544413/1‑93 (MQ=255)

                ggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCt                                                                         <  2:777683/93‑1 (MQ=255)

                ggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCt                                                                         >  1:2906386/1‑93 (MQ=255)

                ggCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCt                                                                         >  2:4748440/1‑93 (MQ=255)

                 gcgcGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCta                                                                        <  1:1684932/93‑1 (MQ=255)

                  cgcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCtat                                                                       <  1:3096031/93‑1 (MQ=255)

                   gcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCtata                                                                      <  1:5266904/93‑1 (MQ=255)

                   gcgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCtata                                                                      <  2:62507/93‑1 (MQ=255)

                    cgGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATaa                                                                     <  1:2616219/93‑1 (MQ=255)

                     ggATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAc                                                                    >  1:6180880/1‑93 (MQ=255)

                       gttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAccc                                                                  <  2:840561/92‑1 (MQ=255)

                       aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAccc                                                                  >  1:2025194/1‑93 (MQ=255)

                       aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAccc                                                                  >  1:5316998/1‑93 (MQ=255)

                       aTTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAAccc                                                                  <  2:3554924/93‑1 (MQ=255)

                        ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCa                                                                 >  2:6336441/1‑93 (MQ=255)

                        ttttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCa                                                                 <  1:6332106/93‑1 (MQ=255)

                         tttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCAc                                                                <  2:2643409/93‑1 (MQ=255)

                          ttGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACt                                                               <  1:2717824/93‑1 (MQ=255)

                           tgtgCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTg                                                              >  2:654144/1‑93 (MQ=255)

                              gCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTa                                                           <  2:2401046/93‑1 (MQ=255)

                               cTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCCGTTGAACGTTTCTATAACCCACTGGTAc                                                          >  2:2162220/1‑93 (MQ=255)

                                tGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACg                                                         >  2:2202980/1‑93 (MQ=255)

                                  gTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTc                                                       >  2:6654784/1‑93 (MQ=255)

                                     aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                    >  2:923909/1‑93 (MQ=255)

                                     aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                    >  2:4813993/1‑93 (MQ=255)

                                     aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                    >  1:1390819/1‑93 (MQ=255)

                                     aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                    <  1:4450217/93‑1 (MQ=255)

                                     aGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                    <  1:3731939/93‑1 (MQ=255)

                                     aGGATCTGAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGcc                                                    <  2:2532219/93‑1 (MQ=255)

                                         tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGc                                                >  2:5353255/1‑93 (MQ=255)

                                         tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGc                                                >  1:3627684/1‑93 (MQ=255)

                                         tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGc                                                <  2:1418228/93‑1 (MQ=255)

                                         tCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGc                                                >  1:4048395/1‑93 (MQ=255)

                                           ttAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCaa                                              >  1:5664509/1‑93 (MQ=255)

                                              aCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAc                                           <  2:2015927/93‑1 (MQ=255)

                                              aCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAc                                           >  1:3813385/1‑93 (MQ=255)

                                              aCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAc                                           <  2:1021101/93‑1 (MQ=255)

                                              aCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAAc                                           >  2:2886887/1‑93 (MQ=255)

                                                 aTCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGc                                        >  1:4244602/1‑93 (MQ=255)

                                                  tCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGcc                                       >  2:6440546/1‑93 (MQ=255)

                                                  tCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGcc                                       <  2:3928157/93‑1 (MQ=255)

                                                  tCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGcc                                       <  2:5609289/93‑1 (MQ=255)

                                                   cGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCa                                      <  2:2472414/93‑1 (MQ=255)

                                                     tAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCaaa                                    >  2:4433440/1‑93 (MQ=255)

                                                       aCGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAacac                                  >  1:1171105/1‑93 (MQ=255)

                                                        cGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCGCTGGTACGTCGCCTGGCAAAACTGCCAAAAcc                                 <  1:1932373/93‑1 (MQ=255)

                                                        cGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAcc                                 >  2:6580814/1‑93 (MQ=255)

                                                        cGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAcc                                 >  1:3191078/1‑93 (MQ=255)

                                                        cGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAcc                                 >  2:1489892/1‑93 (MQ=255)

                                                        cGTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAAcc                                 <  1:4942572/93‑1 (MQ=255)

                                                         gTTGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCg                                <  1:4870763/93‑1 (MQ=255)

                                                          ttGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                               >  1:4128100/1‑93 (MQ=255)

                                                          ttGATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCgg                               >  2:5936488/1‑93 (MQ=255)

                                                            gATCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCggcg                             >  1:4671615/1‑91 (MQ=255)

                                                             aTCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                            >  2:5710616/1‑93 (MQ=255)

                                                             aTCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                            >  2:2309271/1‑93 (MQ=255)

                                                             aTCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGa                            >  1:5391965/1‑93 (MQ=255)

                                                              tCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAt                           <  2:3815452/93‑1 (MQ=255)

                                                              tCCCACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAt                           >  2:5493692/1‑93 (MQ=255)

                                                              acccACAGGCCCCGCACCGGATGTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGAt                           <  2:5233193/92‑1 (MQ=255)

                                                                ccACAGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCt                         >  1:2122822/1‑93 (MQ=255)

                                                                  acaGGCCCTGCACCGGATTCAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtgt                       >  1:6558148/1‑93 (MQ=255)

                                                                  acaGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtgt                       >  1:1618785/1‑93 (MQ=255)

                                                                   caGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCtgtg                      >  1:176620/1‑93 (MQ=255)

                                                                    aGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATACCCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGc                     <  1:2372619/93‑1 (MQ=255)

                                                                    aGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGc                     <  1:6257718/93‑1 (MQ=255)

                                                                    aGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGc                     >  2:4560986/1‑93 (MQ=255)

                                                                    aGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGc                     <  1:1879683/93‑1 (MQ=255)

                                                                    aGGCCCCGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGc                     <  2:1945062/93‑1 (MQ=255)

                                                                       ccccGCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGt                  <  1:2692999/93‑1 (MQ=255)

                                                                           gCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              <  2:1989087/93‑1 (MQ=255)

                                                                           gCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              >  2:5983872/1‑93 (MQ=255)

                                                                           gCACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAt              >  2:1840597/1‑93 (MQ=255)

                                                                            cACCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtg             >  1:4601304/1‑93 (MQ=255)

                                                                             aCCGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtgg            >  2:3291156/1‑93 (MQ=255)

                                                                               cGGATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcg          <  1:5502965/93‑1 (MQ=255)

                                                                                ggATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgt         <  2:406725/93‑1 (MQ=255)

                                                                                ggATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgt         <  2:6032108/93‑1 (MQ=255)

                                                                                ggATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgt         >  2:3045410/1‑93 (MQ=255)

                                                                                ggATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgt         <  1:418489/93‑1 (MQ=255)

                                                                                 gATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtg        <  2:3691471/93‑1 (MQ=255)

                                                                                 gATTTAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtg        <  2:307264/93‑1 (MQ=255)

                                                                                   tttAGGGGTGTCAGTTGAGTGTTTCTATACCCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtggc      <  1:6642675/93‑1 (MQ=255)

                                                                                    ttAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAAtggcgtggcg     <  1:4528254/93‑1 (MQ=255)

                                                                                     tAGGGGTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTggcgg    >  2:4457777/1‑93 (MQ=255)

                                                                                       ggggTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggca  <  1:3876844/93‑1 (MQ=255)

                                                                                       ggggTGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCggca  >  1:5801726/1‑93 (MQ=255)

TTACTTACAGGTGCCGGGCGCGGATTTTGTGCTGGTCAGGATCTTAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCA  >  NC_000913/1456441‑1456620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`