BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,461,082	C→T	P312S (CCA→TCA)	b1398 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,461,082	0	C	T	99.6%	828.6 / NA	223	P312S (CCA→TCA)	b1398	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (121/101); minor base G (0/1); total (121/102)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

GGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCG  >  NC_000913/1460992‑1461171

ggTGAACATGGCGACCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCa                                                                                         <  2:3903267/93‑1 (MQ=255)

ggTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCa                                                                                         <  1:1397995/93‑1 (MQ=255)

ggTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCa                                                                                         >  2:3982904/1‑93 (MQ=255)

ggTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCa                                                                                         >  1:3424337/1‑93 (MQ=255)

ggTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGACATTCGTTACCGCACGCGTGATTTAACCCGCCTGTTATCa                                                                                         >  1:6279661/1‑93 (MQ=255)

 gTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAg                                                                                        >  1:3479505/1‑93 (MQ=255)

  tGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCTACTGTTATCAgg                                                                                       >  1:2439132/1‑93 (MQ=255)

  tGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAgg                                                                                       >  2:526030/1‑93 (MQ=255)

       aTGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACgg                                                                                  >  2:3185572/1‑93 (MQ=255)

        tGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGgc                                                                                 >  1:3128944/1‑93 (MQ=255)

          gCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGgcgc                                                                               <  2:4885228/93‑1 (MQ=255)

          gCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGgcgc                                                                               <  1:5657460/93‑1 (MQ=255)

             aaCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGa                                                                            >  2:2515955/1‑93 (MQ=255)

              aCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCTGAc                                                                           <  2:1236039/93‑1 (MQ=255)

              aCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGAc                                                                           >  2:244809/1‑93 (MQ=255)

               cTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACGCGACTGTTATCAGGAACGGCGCGGACt                                                                          >  2:3527942/1‑93 (MQ=255)

               cTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACt                                                                          >  1:3876081/1‑93 (MQ=255)

               cTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACt                                                                          <  2:241568/93‑1 (MQ=255)

                tGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTa                                                                         >  1:4371075/1‑93 (MQ=255)

                tGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTa                                                                         >  2:4393185/1‑93 (MQ=255)

                tGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTa                                                                         <  1:5168994/93‑1 (MQ=255)

                 gTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTAt                                                                        <  2:2614316/93‑1 (MQ=255)

                 gTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTAt                                                                        >  1:3924664/1‑93 (MQ=255)

                 gTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTAt                                                                        >  2:3058853/1‑93 (MQ=255)

                  ttATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATg                                                                       >  1:2389182/1‑93 (MQ=255)

                  ttATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATg                                                                       >  2:5777800/1‑93 (MQ=255)

                   tATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGTTTTAACCCGACTGTTATCAGGAACGGCGCGGACTATgc                                                                      >  1:4456006/1‑93 (MQ=255)

                   tATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATgc                                                                      >  2:1913707/1‑93 (MQ=255)

                   tATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATgc                                                                      <  1:80604/93‑1 (MQ=255)

                    aTTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATgcg                                                                     >  1:716530/1‑93 (MQ=255)

                      taaccacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGcgcc                                                                   >  1:4199788/3‑93 (MQ=255)

                      tCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGcgcc                                                                   >  1:2574003/1‑93 (MQ=255)

                          cacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCAt                                                               <  2:1458676/93‑1 (MQ=255)

                           acGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATg                                                              <  2:165808/93‑1 (MQ=255)

                           acGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATg                                                              <  1:6090666/93‑1 (MQ=255)

                           acGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATg                                                              >  1:6232544/1‑93 (MQ=255)

                              cTGACCAAAGAAGCATTGCTGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGAt                                                           <  2:2366025/93‑1 (MQ=255)

                                gACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCg                                                         >  2:4508161/1‑93 (MQ=255)

                                 aCCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGc                                                        >  1:2354784/1‑93 (MQ=255)

                                   cAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCAt                                                      <  2:1570941/93‑1 (MQ=255)

                                   cAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCAt                                                      >  2:4767667/1‑93 (MQ=255)

                                   cAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCAt                                                      <  1:1489089/93‑1 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     >  2:1005684/1‑93 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     >  2:6660539/1‑93 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     >  1:46355/1‑93 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     >  1:226416/1‑93 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     >  1:2717950/1‑93 (MQ=255)

                                    aaaGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                     >  1:5442983/1‑93 (MQ=255)

                                       gaagCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGc                                                  >  1:631355/1‑93 (MQ=255)

                                         agCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCCGCgg                                                >  1:2853510/1‑93 (MQ=255)

                                          gCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGa                                               <  1:4006301/93‑1 (MQ=255)

                                          gCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGa                                               <  2:5885545/93‑1 (MQ=255)

                                          gCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGa                                               >  2:1788451/1‑93 (MQ=255)

                                            aTTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACg                                             <  1:3581015/93‑1 (MQ=255)

                                             ttGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGc                                            >  1:2115852/1‑93 (MQ=255)

                                              tGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCa                                           <  2:2792981/93‑1 (MQ=255)

                                              tGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCa                                           >  2:3700724/1‑93 (MQ=255)

                                               gCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAg                                          <  2:4208884/93‑1 (MQ=255)

                                                  ggTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGa                                       >  2:1749038/1‑93 (MQ=255)

                                                  ggTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGa                                       >  2:4808881/1‑93 (MQ=255)

                                                   gTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCCGCGGACGCAGCGAt                                      >  2:3538990/1‑93 (MQ=255)

                                                   gTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGAt                                      <  1:6628493/93‑1 (MQ=255)

                                                     cATTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGa                                    >  1:2078303/1‑93 (MQ=255)

                                                      aTTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGAc                                   <  1:5996727/93‑1 (MQ=255)

                                                      aTTCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGAc                                   >  2:2232343/1‑93 (MQ=255)

                                                       ttCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACa                                  >  2:5811727/1‑93 (MQ=255)

                                                        tCGTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACAt                                 <  1:5717854/93‑1 (MQ=255)

                                                          gTTACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGt                               >  1:6193502/1‑93 (MQ=255)

                                                           ttACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGtt                              <  2:2202587/93‑1 (MQ=255)

                                                           ttACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGtt                              <  2:1958452/93‑1 (MQ=255)

                                                           ttACCGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGtt                              <  2:3433545/93‑1 (MQ=255)

                                                              ccGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGat                           <  2:572243/93‑1 (MQ=255)

                                                               cGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGatt                          <  1:4954397/93‑1 (MQ=255)

                                                               cGCACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGatt                          >  2:4667442/1‑93 (MQ=255)

                                                                 cACGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGattat                        <  2:2143738/93‑1 (MQ=255)

                                                                  aCGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGattatt                       >  2:6259729/1‑93 (MQ=255)

                                                                  aCGCGTGATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGattatt                       <  2:4282106/93‑1 (MQ=255)

                                                                        gATTTAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgt                 <  2:4966283/93‑1 (MQ=255)

                                                                          tttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtgt               >  1:1796669/1‑93 (MQ=255)

                                                                          tttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtgt               <  2:6528015/93‑1 (MQ=255)

                                                                           ttAACCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTa              >  2:4047274/1‑93 (MQ=255)

                                                                             aaCCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTaaa            <  2:1565725/93‑1 (MQ=255)

                                                                              aCCCGACTGTTATCCGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAAt           <  1:1106830/93‑1 (MQ=255)

                                                                              aCCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAAt           >  2:6007355/1‑93 (MQ=255)

                                                                              aCCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAAt           >  1:6054697/1‑93 (MQ=255)

                                                                              aCCCGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAAt           <  1:5882502/93‑1 (MQ=255)

                                                                               cccGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATg          >  1:1033102/1‑93 (MQ=255)

                                                                                ccGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCTCGGTGTAAATGt         <  2:3592596/93‑1 (MQ=255)

                                                                                ccGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGt         <  2:1355018/93‑1 (MQ=255)

                                                                                 cGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTc        <  2:2017413/93‑1 (MQ=255)

                                                                                 cGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTc        <  2:5564367/93‑1 (MQ=255)

                                                                                 cGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTc        >  2:5467935/1‑93 (MQ=255)

                                                                                 cGACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTc        <  2:5209030/93‑1 (MQ=255)

                                                                                  gACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCt       <  1:531039/93‑1 (MQ=255)

                                                                                  gACTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCt       <  1:159215/93‑1 (MQ=255)

                                                                                   aCTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCtt      <  1:3824337/93‑1 (MQ=255)

                                                                                   aCTGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCtt      >  1:2693584/1‑93 (MQ=255)

                                                                                     tGTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTc    >  1:4777609/1‑93 (MQ=255)

                                                                                      gTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTcc   <  1:6697674/93‑1 (MQ=255)

                                                                                      gTTATCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTcc   >  2:672540/1‑93 (MQ=255)

GGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCG  >  NC_000913/1460992‑1461171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`