BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,484,846	A→G	A1254A (GCA→GCG)	hrpA →	helicase, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,484,846	0	A	G	99.5%	634.5 / ‑4.7	199	A1254A (GCA→GCG)	hrpA	helicase, ATP‑dependent
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (103/95); total (104/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 207 total aligned reads displayed.

GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTA  >  NC_000913/1484756‑1484935

ggTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCcgcgcg                                                                                         >  1:1951912/1‑93 (MQ=255)

 gTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgt                                                                                        <  1:2879435/93‑1 (MQ=255)

  ttGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtc                                                                                       >  1:1738744/1‑93 (MQ=255)

   tGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGcgtcg                                                                                      <  2:5970939/93‑1 (MQ=255)

     aTCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTg                                                                                    >  2:3336888/1‑93 (MQ=255)

       ccACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAg                                                                                  >  2:1921679/1‑93 (MQ=255)

        cacaTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAgg                                                                                 >  2:6092853/1‑93 (MQ=255)

         ccaTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGa                                                                                <  1:1118928/92‑1 (MQ=255)

         acaTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACCAACTGCCGCCCGCGCGTCGTGAGGa                                                                                >  1:5939597/1‑93 (MQ=255)

          caTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAt                                                                               <  1:3268537/93‑1 (MQ=255)

          caTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGAt                                                                               >  2:6415080/1‑93 (MQ=255)

           aTCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATg                                                                              <  2:2878018/93‑1 (MQ=255)

            tCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGa                                                                             <  1:3728223/93‑1 (MQ=255)

            tCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGa                                                                             <  1:6699525/93‑1 (MQ=255)

            tCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGa                                                                             >  1:5571737/1‑93 (MQ=255)

               cgACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGa                                                                          <  1:1816850/93‑1 (MQ=255)

               cgACCGTGCGCACATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCACGCGTCGTGAGGATGAAGa                                                                          <  2:6114531/93‑1 (MQ=255)

                gACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGAc                                                                         <  2:2600644/93‑1 (MQ=255)

                gACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGAc                                                                         <  2:3101955/93‑1 (MQ=255)

                 aCCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACg                                                                        <  2:2323876/93‑1 (MQ=255)

                   cGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTg                                                                      >  1:3179067/1‑93 (MQ=255)

                   cGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTg                                                                      >  2:172868/1‑93 (MQ=255)

                    gTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGa                                                                     >  1:2177938/1‑93 (MQ=255)

                     tGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGaa                                                                    >  2:1763227/1‑93 (MQ=255)

                       cgcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAag                                                                  <  1:3939313/93‑1 (MQ=255)

                        gcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAaga                                                                 >  2:4629069/1‑93 (MQ=255)

                        gcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAaga                                                                 <  2:3271451/93‑1 (MQ=255)

                        gcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAaga                                                                 >  1:1711345/1‑93 (MQ=255)

                        gcAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAaga                                                                 >  2:3433905/1‑93 (MQ=255)

                          aGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAgaga                                                               <  2:4986397/93‑1 (MQ=255)

                           gATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGAGAGGATGAAGACGTGAAAGAGAt                                                              >  1:1140247/1‑93 (MQ=255)

                             tGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATcc                                                            <  1:4090546/93‑1 (MQ=255)

                              gCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCg                                                           >  2:4743850/1‑93 (MQ=255)

                               cTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGt                                                          <  2:4894581/93‑1 (MQ=255)

                               cTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGt                                                          <  2:6533813/93‑1 (MQ=255)

                                tGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCTGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGtt                                                         <  1:6514396/93‑1 (MQ=255)

                                tGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGtt                                                         >  2:4207381/1‑93 (MQ=255)

                                tGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGtt                                                         >  1:5360684/1‑93 (MQ=255)

                                 gAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTg                                                        >  1:4784625/1‑93 (MQ=255)

                                  aaaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTgg                                                       >  1:1272355/1‑93 (MQ=255)

                                   aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                      <  2:6395981/93‑1 (MQ=255)

                                   aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGga                                                      <  1:6101493/93‑1 (MQ=255)

                                   aaGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGACATCAGTTGga                                                      >  2:1361812/1‑93 (MQ=255)

                                     gTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatg                                                    >  1:1363023/1‑93 (MQ=255)

                                     gTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatg                                                    >  2:2356479/1‑93 (MQ=255)

                                     gTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatg                                                    >  2:5370870/1‑93 (MQ=255)

                                     gTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatg                                                    <  1:2065691/93‑1 (MQ=255)

                                      tCGAGACCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatga                                                   <  1:1525535/93‑1 (MQ=255)

                                       cGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGgatgat                                                  <  1:916253/93‑1 (MQ=255)

                                         aaaaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATag                                                <  1:5329392/93‑1 (MQ=255)

                                           aaCGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATagaa                                              <  1:4802730/93‑1 (MQ=255)

                                               tCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGt                                          <  1:4226110/93‑1 (MQ=255)

                                               tCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGt                                          <  1:2655274/93‑1 (MQ=255)

                                               tCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGt                                          >  2:5031753/1‑93 (MQ=255)

                                                ccAGCAGGCGTGGCAGCAATGGATCAACAAACTTCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGtt                                         <  2:5910613/93‑1 (MQ=255)

                                                ccAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGtt                                         <  1:718371/93‑1 (MQ=255)

                                                  aggagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       <  2:130281/90‑1 (MQ=255)

                                                  agcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgc                                       <  2:4459802/93‑1 (MQ=255)

                                                   gcagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgcg                                      >  1:6408499/1‑93 (MQ=255)

                                                    cagGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTgcgc                                     >  2:1028196/1‑93 (MQ=255)

                                                     agGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTTGATGATAGAAGAGTTGcgcg                                    >  2:5089888/1‑93 (MQ=255)

                                                     agGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGcgcg                                    >  1:349739/1‑93 (MQ=255)

                                                     agGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGcgcg                                    >  1:547854/1‑93 (MQ=255)

                                                      ggCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgt                                   >  1:5017576/1‑93 (MQ=255)

                                                      ggCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgt                                   <  1:3579794/93‑1 (MQ=255)

                                                        cGTGGCAGCGATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAACGCGATCCGTTGGATGATAGAAGAGTTGCGCgtta                                 <  2:6721974/93‑1 (MQ=255)

                                                          tGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagt                               >  1:1908268/1‑93 (MQ=255)

                                                          tGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagt                               <  2:2847310/93‑1 (MQ=255)

                                                          tGGCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagt                               >  2:6300864/1‑93 (MQ=255)

                                                           ggCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagtt                              <  1:6003932/93‑1 (MQ=255)

                                                           ggCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagtt                              >  2:4752307/1‑93 (MQ=255)

                                                           ggCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagtt                              <  2:4028330/93‑1 (MQ=255)

                                                           ggCAGCAATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCgttagtt                              <  1:4453215/93‑1 (MQ=255)

                                                             cagcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTAc                            <  2:3833416/93‑1 (MQ=255)

                                                             cagcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTAc                            >  1:6224433/1‑93 (MQ=255)

                                                              agcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACt                           <  2:5610530/93‑1 (MQ=255)

                                                              agcaATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACt                           <  1:859712/93‑1 (MQ=255)

                                                                caATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTc                         <  1:2197353/93‑1 (MQ=255)

                                                                caATGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTc                         <  2:256855/93‑1 (MQ=255)

                                                                  aTGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGc                       >  1:491242/1‑93 (MQ=255)

                                                                   tGGATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCt                      >  2:4016878/1‑93 (MQ=255)

                                                                     gATCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTca                    >  1:2447157/1‑93 (MQ=255)

                                                                      aTCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaa                   >  1:5704761/1‑93 (MQ=255)

                                                                       tCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaac                  >  2:293181/1‑93 (MQ=255)

                                                                       tCAACAAACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaac                  <  2:5154510/93‑1 (MQ=255)

                                                                        caacaaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaaca                 >  1:4637716/1‑93 (MQ=255)

                                                                         aacaaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTcaacaa                >  1:5808192/1‑93 (MQ=255)

                                                                          acaaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCaacaac               <  2:6031398/93‑1 (MQ=255)

                                                                           caaACTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTGCTTCGCTCAACAACt              <  2:741308/93‑1 (MQ=255)

                                                                            aaaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACtt             >  1:1459990/1‑93 (MQ=255)

                                                                             aaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTg            >  2:6363049/1‑93 (MQ=255)

                                                                             aaCTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTg            >  2:2995244/1‑93 (MQ=255)

                                                                               cTGCCGCCCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGt          <  1:411084/93‑1 (MQ=255)

                                                                                 gccgccCGCGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTAc        <  2:3723471/93‑1 (MQ=255)

                                                                                    gcccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGcc     <  1:3231942/93‑1 (MQ=255)

                                                                                    gcccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGcc     >  2:1420561/1‑93 (MQ=255)

                                                                                     cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCt    >  2:5634854/1‑93 (MQ=255)

                                                                                     cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCt    >  2:3627386/1‑93 (MQ=255)

                                                                                     cccgcGCGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCt    >  1:104744/1‑93 (MQ=255)

                                                                                       cgcgcgTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTa  <  1:2772867/93‑1 (MQ=255)

GGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGACGTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTTGGTACGCCTTA  >  NC_000913/1484756‑1484935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`