BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,599	T→C	H292R (CAC→CGC)	b1428 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,599	0	T	C	99.5%	681.3 / ‑4.7	211	H292R (CAC→CGC)	b1428	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (97/113); total (97/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.80e-01

Only 100 of 223 total aligned reads displayed.

AGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATC  >  NC_000913/1497509‑1497688

cgcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCgg                                                                                         <  1:3427934/92‑1 (MQ=255)

aGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCgg                                                                                         <  2:2158687/93‑1 (MQ=255)

aGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCgg                                                                                         <  2:4720285/93‑1 (MQ=255)

 gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        <  1:5338993/93‑1 (MQ=255)

 gcggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGt                                                                                        >  1:615194/1‑93 (MQ=255)

   ggcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCg                                                                                      <  2:2298280/93‑1 (MQ=255)

    gcgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCgg                                                                                     >  1:472818/1‑93 (MQ=255)

     cgTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGcggtcggt                                                                                    <  2:1875186/93‑1 (MQ=255)

      gTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGtt                                                                                   >  2:3192623/1‑93 (MQ=255)

       ttCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTa                                                                                  >  2:6274077/1‑93 (MQ=255)

        tCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTaa                                                                                 <  2:2753620/93‑1 (MQ=255)

         cgcgTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAAc                                                                                <  2:504420/93‑1 (MQ=255)

          gcgTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACt                                                                               <  1:2732282/93‑1 (MQ=255)

           cgTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTc                                                                              <  1:3894798/93‑1 (MQ=255)

            gTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTcc                                                                             <  2:882774/93‑1 (MQ=255)

            gTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTcc                                                                             <  1:1464238/93‑1 (MQ=255)

               aTGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCcaca                                                                          <  2:1598744/93‑1 (MQ=255)

                  cgcTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATgg                                                                       <  2:3063818/93‑1 (MQ=255)

                    ctcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGtt                                                                     <  1:6313745/93‑1 (MQ=255)

                    ctcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGtt                                                                     <  1:2657449/93‑1 (MQ=255)

                    ctcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGtt                                                                     <  1:2845826/93‑1 (MQ=255)

                    ctcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGtt                                                                     <  1:1635575/93‑1 (MQ=255)

                     tcctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGttt                                                                    >  1:1103709/1‑93 (MQ=255)

                      cctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGtttt                                                                   <  2:4574548/93‑1 (MQ=255)

                      cctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGtttt                                                                   >  1:1319555/1‑93 (MQ=255)

                      cctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGcttt                                                                   >  1:642224/1‑93 (MQ=255)

                       ctcGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTc                                                                  <  2:265233/93‑1 (MQ=255)

                         cGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCaa                                                                <  1:4363943/93‑1 (MQ=255)

                          gCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAAt                                                               <  2:5474515/93‑1 (MQ=255)

                           ccGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAAtt                                                              <  2:592688/93‑1 (MQ=255)

                           ccGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAAtt                                                              <  2:4461784/93‑1 (MQ=255)

                            cGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAAttt                                                             >  2:6359809/1‑92 (MQ=255)

                            cGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTa                                                             <  2:1398105/93‑1 (MQ=255)

                            cGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTa                                                             >  1:4058249/1‑93 (MQ=255)

                            cGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTa                                                             <  1:3122726/93‑1 (MQ=255)

                             gTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTAt                                                            >  1:3540483/1‑93 (MQ=255)

                             gTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTAt                                                            >  2:3023550/1‑93 (MQ=255)

                             gTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTAt                                                            >  1:5419286/1‑93 (MQ=255)

                              ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           <  1:6114339/93‑1 (MQ=255)

                              ttAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATc                                                           <  1:3104672/93‑1 (MQ=255)

                                  tGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCg                                                       >  2:4772552/1‑93 (MQ=255)

                                  tGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCg                                                       >  1:1387609/1‑93 (MQ=255)

                                   gACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGc                                                      >  2:3133746/1‑93 (MQ=255)

                                    aCTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCa                                                     >  2:1275634/1‑93 (MQ=255)

                                     cTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGGTTTCAATTATCACCGCAc                                                    >  1:1604196/1‑93 (MQ=255)

                                            aCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAAt                                             >  1:2237390/1‑93 (MQ=255)

                                            aCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAAt                                             <  2:3300981/93‑1 (MQ=255)

                                            aCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAAt                                             <  1:4984334/93‑1 (MQ=255)

                                             ccACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATa                                            <  1:6491780/93‑1 (MQ=255)

                                             ccACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATa                                            >  1:624092/1‑93 (MQ=255)

                                               aCGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACg                                          <  2:3280184/93‑1 (MQ=255)

                                               aCGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACg                                          >  1:1340893/1‑93 (MQ=255)

                                               aCGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACg                                          <  1:1154862/93‑1 (MQ=255)

                                                cGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACgg                                         <  1:5694804/93‑1 (MQ=255)

                                                  gACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTTAATTATCACCGCACCTGTAATACGGct                                       >  2:5387239/1‑93 (MQ=255)

                                                   aCATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGctc                                      <  1:1823744/93‑1 (MQ=255)

                                                    cATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGctct                                     >  2:4308112/1‑93 (MQ=255)

                                                    cATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGctct                                     >  1:314726/1‑93 (MQ=255)

                                                      tGAACCGTGTCGCCATCAAAGTCTTCAGCGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCa                                   <  1:21002/93‑1 (MQ=255)

                                                       gAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCAc                                  >  2:6691631/1‑93 (MQ=255)

                                                        aaCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCAcc                                 <  2:3226807/93‑1 (MQ=255)

                                                        aaCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCAcc                                 <  2:255130/93‑1 (MQ=255)

                                                         aCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCt                                >  2:5309005/1‑93 (MQ=255)

                                                         aCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCt                                >  1:822735/1‑93 (MQ=255)

                                                         aCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCt                                >  1:364284/1‑93 (MQ=255)

                                                         aCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCt                                >  2:3925275/1‑93 (MQ=255)

                                                         aCCGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCt                                <  1:6696082/93‑1 (MQ=255)

                                                          ccGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCtt                               <  1:1766472/93‑1 (MQ=255)

                                                          ccGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCtt                               >  1:5593500/1‑93 (MQ=255)

                                                          ccGTGTCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCtt                               <  2:5679388/93‑1 (MQ=255)

                                                            gtgtCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGt                             >  2:5603055/1‑93 (MQ=255)

                                                            gtgtCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGt                             >  2:5444763/1‑93 (MQ=255)

                                                             tgtCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTa                            <  1:3174347/93‑1 (MQ=255)

                                                             tgtCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTa                            <  2:6191532/93‑1 (MQ=255)

                                                               tCGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATg                          >  1:4192743/1‑93 (MQ=255)

                                                                cGCCATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGa                         <  1:574211/93‑1 (MQ=255)

                                                                  ccATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACATTGTATGACa                       >  1:2363468/1‑93 (MQ=255)

                                                                   cATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACaa                      <  2:3586530/93‑1 (MQ=255)

                                                                   cATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACaa                      >  2:4024484/1‑93 (MQ=255)

                                                                   cATCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACaa                      >  2:2076566/1‑93 (MQ=255)

                                                                    aTCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAAc                     >  1:4875231/1‑93 (MQ=255)

                                                                    aTCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAAc                     >  1:3649313/1‑93 (MQ=255)

                                                                    aTCAAAGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAAc                     >  2:758149/1‑93 (MQ=255)

                                                                         aGGCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTACTACGGCTCTCACCTTGTATGACAACGTGCt                >  2:6340252/1‑93 (MQ=255)

                                                                          ggCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtc               <  1:2679894/93‑1 (MQ=255)

                                                                           gCTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcg              <  2:6518756/93‑1 (MQ=255)

                                                                            cTTCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCATCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcgt             >  1:6167812/1‑93 (MQ=255)

                                                                              tCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcgtcg           >  2:108443/1‑93 (MQ=255)

                                                                              tCAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCtcgtcg           <  2:1720974/93‑1 (MQ=255)

                                                                               cAACGACGGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCCCCGCACCTGTAATACGGCTCTCCCCTTGTATGACAACGTGCTCGTCGa          <  2:2638306/93‑1 (MQ=255)

                                                                                 acgacgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGc        >  2:3482646/1‑93 (MQ=255)

                                                                                 acgacgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGc        >  2:2208419/1‑93 (MQ=255)

                                                                                  cgacgGCGCGGTCGGTTAACTCCACATGGTTTTCTATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCa       >  2:3854744/1‑93 (MQ=255)

                                                                                   gacgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAg      >  1:6025313/1‑93 (MQ=255)

                                                                                    acgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGa     >  1:1053305/1‑93 (MQ=255)

                                                                                    acgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGa     >  2:4679025/1‑93 (MQ=255)

                                                                                     cgGCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGaa    <  2:1204316/93‑1 (MQ=255)

                                                                                      ggCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAAt   <  1:4939515/93‑1 (MQ=255)

                                                                                      ggCGCGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAAt   <  2:5150518/93‑1 (MQ=255)

                                                                                       gcgcGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATc  <  1:3352846/93‑1 (MQ=255)

AGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATC  >  NC_000913/1497509‑1497688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`