BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,563,111	G→A	T224M (ACG→ATG)	b1489 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,563,111	0	G	A	99.0%	701.4 / ‑5.9	190	T224M (ACG→ATG)	b1489	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (82/106); total (83/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

GAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATG  >  NC_000913/1563021‑1563200

gagaAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGCTAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATc                                                                                         >  2:5641854/1‑93 (MQ=255)

gagaAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTc                                                                                         >  2:709492/1‑93 (MQ=255)

 agaAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCa                                                                                        >  2:3182523/1‑93 (MQ=255)

 agaAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCa                                                                                        <  2:4010384/93‑1 (MQ=255)

   aaaGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAAc                                                                                      <  2:5680929/93‑1 (MQ=255)

   aaaGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAAc                                                                                      <  1:627226/93‑1 (MQ=255)

   aaaGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAAc                                                                                      <  2:4933987/93‑1 (MQ=255)

      gTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAAcagc                                                                                   <  1:6319831/93‑1 (MQ=255)

       tCATTACCAGTTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAAcagca                                                                                  <  2:2634216/93‑1 (MQ=255)

       tCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCGGATTTTTTCACCGGTGCGCATCAAcagca                                                                                  <  2:6396906/93‑1 (MQ=255)

       tCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAAcagca                                                                                  >  2:2326170/1‑93 (MQ=255)

       tCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAAcagca                                                                                  <  1:3314127/93‑1 (MQ=255)

        cATTACCAGGTTCTGCAGATGCGCGAGCACGTCATGAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAAcagcag                                                                                 <  1:1045684/93‑1 (MQ=255)

        cATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAAcagcag                                                                                 <  1:109013/93‑1 (MQ=255)

         aTTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGa                                                                                <  1:1362407/93‑1 (MQ=255)

            aCCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAAt                                                                             >  1:4677173/1‑93 (MQ=255)

             ccAGGTTCTGCAGATGCGCGAGCACGTCATTAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAAtt                                                                            >  1:847114/1‑93 (MQ=255)

             ccAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAAtt                                                                            >  2:3047078/1‑93 (MQ=255)

              cAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTc                                                                           >  1:1901123/1‑93 (MQ=255)

              cAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTc                                                                           >  1:3750841/1‑93 (MQ=255)

              cAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTc                                                                           >  1:4488388/1‑93 (MQ=255)

                  ttCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTcc                                                                       <  1:1039786/93‑1 (MQ=255)

                   tCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCt                                                                      <  2:4467884/93‑1 (MQ=255)

                   tCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCt                                                                      >  2:385895/1‑93 (MQ=255)

                   tCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCt                                                                      <  1:2775942/93‑1 (MQ=255)

                      gCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGAt                                                                   >  2:5837302/1‑93 (MQ=255)

                      gCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGAt                                                                   <  2:5214950/93‑1 (MQ=255)

                        aGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATcg                                                                 >  1:5391606/1‑93 (MQ=255)

                          aTGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATcgcg                                                               <  1:3990011/93‑1 (MQ=255)

                            gcgcGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCgcggg                                                             >  1:5899155/1‑93 (MQ=255)

                               cgAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCgcgggcgg                                                          >  1:2422206/1‑93 (MQ=255)

                               cgAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCgcgggcgg                                                          >  2:5967082/1‑93 (MQ=255)

                                gAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGt                                                         <  2:3386619/93‑1 (MQ=255)

                                   cACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGtttt                                                      >  1:927252/1‑93 (MQ=255)

                                    aCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTc                                                     >  2:2602599/1‑93 (MQ=255)

                                    aCGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTc                                                     >  2:1566332/1‑93 (MQ=255)

                                      gTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCa                                                   <  1:2556363/93‑1 (MQ=255)

                                      gTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCa                                                   <  1:4850682/93‑1 (MQ=255)

                                      gTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCa                                                   <  1:2387098/93‑1 (MQ=255)

                                       tCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCAt                                                  <  2:3695661/93‑1 (MQ=255)

                                        cATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATa                                                 <  2:3787710/93‑1 (MQ=255)

                                        cATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATa                                                 <  1:678309/93‑1 (MQ=255)

                                        cATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATa                                                 >  1:4282463/1‑93 (MQ=255)

                                               cGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACtg                                          <  2:543469/93‑1 (MQ=255)

                                                gggCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACtgt                                         >  1:789642/1‑93 (MQ=255)

                                                aggCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGAGCGCGGGCGGTTTTCCATAGCAACtgt                                         <  1:4180778/92‑1 (MQ=255)

                                                  gCTGATAGAGGCTTTAATCCAGCTTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCTTGATCGCGGGCGGTTTTCCATAGCAACtgttg                                       >  1:4416437/1‑93 (MQ=255)

                                                   cTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACtgttgt                                      >  2:2874495/1‑93 (MQ=255)

                                                   cTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACtgttgt                                      <  1:3263173/93‑1 (MQ=255)

                                                    tGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTa                                     >  1:2254712/1‑93 (MQ=255)

                                                    tGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTa                                     >  2:1859748/1‑93 (MQ=255)

                                                    tGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTa                                     >  1:2004861/1‑93 (MQ=255)

                                                     gaaagagACATTAATACAGATTTTTACACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTaa                                    <  1:117806/90‑1 (MQ=255)

                                                     gATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTaa                                    >  1:1216337/1‑93 (MQ=255)

                                                      aTAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTaaa                                   <  2:2459376/93‑1 (MQ=255)

                                                       tAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAAc                                  >  1:4666650/1‑93 (MQ=255)

                                                         gagGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGa                                <  1:4484662/93‑1 (MQ=255)

                                                         gagGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGa                                >  2:812755/1‑93 (MQ=255)

                                                         gagGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGa                                <  1:1164561/93‑1 (MQ=255)

                                                         gagGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGa                                <  1:5453628/93‑1 (MQ=255)

                                                          agGCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGaa                               <  1:3689362/93‑1 (MQ=255)

                                                           ggCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAAt                              >  1:4843267/1‑93 (MQ=255)

                                                           ggCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGCTGTAAACGAAt                              >  1:5499407/1‑93 (MQ=255)

                                                            gCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATg                             >  1:2709676/1‑93 (MQ=255)

                                                            gCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATg                             <  2:3948619/93‑1 (MQ=255)

                                                            gCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATg                             >  2:5008629/1‑93 (MQ=255)

                                                            gCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATg                             >  2:5225016/1‑93 (MQ=255)

                                                            gCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATg                             <  1:5619571/93‑1 (MQ=255)

                                                            gCTTTAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATg                             >  2:2525230/1‑93 (MQ=255)

                                                              tttAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCg                           >  1:2962266/1‑93 (MQ=255)

                                                                tAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGt                         >  2:536345/1‑93 (MQ=255)

                                                                tAATCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGt                         <  1:1581504/93‑1 (MQ=255)

                                                                 aaTCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGtt                        >  1:5757857/1‑93 (MQ=255)

                                                                 aaTCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGtt                        <  1:5786421/93‑1 (MQ=255)

                                                                 aaTCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGtt                        >  2:4882425/1‑93 (MQ=255)

                                                                  aTCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTa                       <  2:5409543/93‑1 (MQ=255)

                                                                   tCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTAt                      <  1:3155239/93‑1 (MQ=255)

                                                                   tCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTAt                      >  2:2707821/1‑93 (MQ=255)

                                                                   tCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTAt                      >  1:5134448/1‑93 (MQ=255)

                                                                   tCCAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTAt                      >  2:5750694/1‑93 (MQ=255)

                                                                     cAGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCg                    <  1:1597085/93‑1 (MQ=255)

                                                                      aGATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCgg                   >  2:3282688/1‑93 (MQ=255)

                                                                       gATTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGc                  >  1:4347965/1‑93 (MQ=255)

                                                                        aTTTTTTCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCa                 <  2:5231528/93‑1 (MQ=255)

                                                                          tttttCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAgg               <  1:1800296/93‑1 (MQ=255)

                                                                           ttttCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAggg              <  1:721299/93‑1 (MQ=255)

                                                                           ttttCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAggg              <  2:6027279/93‑1 (MQ=255)

                                                                            tttCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGa             <  1:3819541/93‑1 (MQ=255)

                                                                            tttCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGa             >  1:3383000/1‑93 (MQ=255)

                                                                             ttCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGaa            <  2:927114/93‑1 (MQ=255)

                                                                              tCACCGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAAt           <  1:1917020/93‑1 (MQ=255)

                                                                                  cGGTGCGCGTCGCCAGCAGAACTTCGTCCTGATCGCGGGCGGTTTTCCATAGCCCCTGTTGTCCCCGAATGCGGTTATCGGCCGGGCATTCAg       <  1:6727482/93‑1 (MQ=255)

                                                                                  cGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAg       <  2:1633739/93‑1 (MQ=255)

                                                                                  cGGTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAg       <  2:2134579/93‑1 (MQ=255)

                                                                                    gTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGa     <  2:3767024/93‑1 (MQ=255)

                                                                                    gTGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGa     >  2:2857180/1‑93 (MQ=255)

                                                                                     tGCGCATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGaa    <  2:1073549/93‑1 (MQ=255)

                                                                                     ggcgcATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGaa    <  2:6227853/92‑1 (MQ=255)

                                                                                      gcgcATCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAAt   >  1:3043728/1‑93 (MQ=255)

                                                                                       cgcaTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATg  <  2:4659551/93‑1 (MQ=255)

GAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATG  >  NC_000913/1563021‑1563200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`