BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,245	T→C	L91L (TTA→TTG)	ynfC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,245	0	T	C	99.6%	822.2 / NA	248	L91L (TTA→TTG)	ynfC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (130/117); minor base G (1/0); total (131/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGT  >  NC_000913/1655155‑1655334

  aaCGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGCGCGACCACGGTATTATTTTCCAGATCCAGCAAtt                                                                                       >  1:5528070/1‑93 (MQ=255)

   aCGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTc                                                                                      >  2:126236/1‑93 (MQ=255)

   aCGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTc                                                                                      <  2:162109/93‑1 (MQ=255)

   aCGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTc                                                                                      >  2:4998814/1‑93 (MQ=255)

    cGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCg                                                                                     <  2:4546697/93‑1 (MQ=255)

    cGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCg                                                                                     >  1:1706298/1‑93 (MQ=255)

     gTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGa                                                                                    <  1:1006297/93‑1 (MQ=255)

      tACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAg                                                                                   >  1:4257461/1‑93 (MQ=255)

       aCTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGt                                                                                  <  1:6552364/93‑1 (MQ=255)

           tcttctCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAAt                                                                              >  1:3329142/1‑93 (MQ=255)

            cttctCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATc                                                                             <  2:3235606/93‑1 (MQ=255)

               ctcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaaa                                                                          >  2:4082255/1‑93 (MQ=255)

                 ccAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAAc                                                                        <  1:246409/93‑1 (MQ=255)

                 ccAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAAc                                                                        <  2:2562948/93‑1 (MQ=255)

                   aGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAg                                                                      >  1:4722314/1‑93 (MQ=255)

                    gCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGc                                                                     >  2:6718541/1‑93 (MQ=255)

                    gCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGc                                                                     <  2:3143597/93‑1 (MQ=255)

                    gCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGc                                                                     >  1:6044747/1‑93 (MQ=255)

                      gTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCct                                                                   >  1:1515926/1‑93 (MQ=255)

                      gTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCct                                                                   <  1:846852/93‑1 (MQ=255)

                      gTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCct                                                                   <  2:6439233/93‑1 (MQ=255)

                      gTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCct                                                                   <  2:5471601/93‑1 (MQ=255)

                       tctcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCctt                                                                  >  1:4577418/1‑93 (MQ=255)

                       tctcGGCATCACGGTAATAATTGGCGGCCAGTACCAGAGCTACCACGGTATTATTTGCCAGATCCAGCAATTCGAGTGAATCAAAACAGCctt                                                                  <  1:3024049/93‑1 (MQ=255)

                        ctcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCcttc                                                                 >  1:3641897/1‑93 (MQ=255)

                         tcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCcttct                                                                <  2:3097393/93‑1 (MQ=255)

                         tcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCcttct                                                                <  1:4096894/93‑1 (MQ=255)

                         tcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCcttct                                                                <  1:243150/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                              <  1:2019592/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                              >  1:3699112/1‑93 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                              <  1:6010923/93‑1 (MQ=255)

                            gCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTcc                                                             <  1:1154347/93‑1 (MQ=255)

                            gCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTcc                                                             >  1:5768561/1‑93 (MQ=255)

                              aTCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGa                                                           >  2:6375910/1‑93 (MQ=255)

                              aTCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGa                                                           >  2:1493370/1‑93 (MQ=255)

                              aTCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGa                                                           <  2:6203081/93‑1 (MQ=255)

                                  cGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACaaa                                                       <  1:4063095/93‑1 (MQ=255)

                                   ggTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAg                                                      >  2:847997/1‑93 (MQ=255)

                                   ggTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTCCCGACAAAg                                                      >  1:2732124/1‑93 (MQ=255)

                                    gTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGt                                                     <  1:1109132/93‑1 (MQ=255)

                                    gTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGt                                                     <  1:1261859/93‑1 (MQ=255)

                                    gTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGt                                                     <  1:5288865/93‑1 (MQ=255)

                                     taataaTTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTc                                                    >  1:2862958/1‑93 (MQ=255)

                                       ataatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTccc                                                  >  1:76060/1‑93 (MQ=255)

                                        taatTGGCGTCCAGTACCAGCGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTcaca                                                 >  2:1709416/1‑93 (MQ=255)

                                          atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGa                                               >  2:3860777/1‑93 (MQ=255)

                                          atTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGa                                               <  2:2803224/93‑1 (MQ=255)

                                           ttGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGaa                                              >  2:2951154/1‑93 (MQ=255)

                                           ttGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGaa                                              >  1:1188198/1‑93 (MQ=255)

                                              gCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAaca                                           <  2:1994191/93‑1 (MQ=255)

                                              gCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAaca                                           <  2:1000157/93‑1 (MQ=255)

                                               cGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAacac                                          <  1:1094461/93‑1 (MQ=255)

                                                gTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACg                                         >  2:1375536/1‑93 (MQ=255)

                                                  ccAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGtt                                       <  2:3435559/93‑1 (MQ=255)

                                                  ccAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGtt                                       <  1:5104349/93‑1 (MQ=255)

                                                   cAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGttt                                      <  2:495219/93‑1 (MQ=255)

                                                    aGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc                                     >  2:6210839/1‑93 (MQ=255)

                                                    aGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc                                     <  2:6549570/93‑1 (MQ=255)

                                                     gTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCg                                    >  2:5056635/1‑93 (MQ=255)

                                                     gTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCg                                    >  1:3234200/1‑93 (MQ=255)

                                                      tACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGt                                   <  2:2310895/93‑1 (MQ=255)

                                                       aCCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTc                                  >  1:606578/1‑93 (MQ=255)

                                                        ccAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCa                                 >  1:5804575/1‑93 (MQ=255)

                                                          agagCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACt                               >  2:1956853/1‑93 (MQ=255)

                                                            agCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTc                             <  2:3345307/93‑1 (MQ=255)

                                                                aCCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCt                         >  2:3535676/1‑93 (MQ=255)

                                                                aCCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCt                         <  2:928085/93‑1 (MQ=255)

                                                                aCCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCt                         <  1:3063734/93‑1 (MQ=255)

                                                                aCCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCt                         <  1:4226117/93‑1 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                      <  1:5476762/93‑1 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                      >  2:3948542/1‑93 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                      >  2:5721908/1‑93 (MQ=255)

                                                                    cGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCt                     >  2:5084234/1‑93 (MQ=255)

                                                                    cGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCt                     >  2:3613403/1‑93 (MQ=255)

                                                                     ggTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACCAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTc                    >  1:2315439/1‑93 (MQ=255)

                                                                     ggTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTc                    <  2:2188556/93‑1 (MQ=255)

                                                                      gTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCa                   >  2:6621845/1‑93 (MQ=255)

                                                                      gTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCa                   >  1:4896395/1‑93 (MQ=255)

                                                                      gTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCa                   <  1:4998721/93‑1 (MQ=255)

                                                                       tattatTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAt                  <  1:1587240/93‑1 (MQ=255)

                                                                       tattatTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAt                  >  1:6320669/1‑93 (MQ=255)

                                                                        attattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATc                 <  1:4847911/93‑1 (MQ=255)

                                                                         ttattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATcc                >  2:766865/1‑93 (MQ=255)

                                                                           attTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCAt              >  2:4485834/1‑93 (MQ=255)

                                                                              ttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTaa           <  2:2688022/93‑1 (MQ=255)

                                                                              ttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTaa           <  1:6607156/93‑1 (MQ=255)

                                                                                ccAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATg         <  1:1343570/93‑1 (MQ=255)

                                                                                 cAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGt        >  2:5300669/1‑93 (MQ=255)

                                                                                  aGATCCAGCAATTCGAGTGCATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTc       >  1:6506883/1‑93 (MQ=255)

                                                                                  aGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTc       >  2:4144066/1‑93 (MQ=255)

                                                                                  aGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTc       >  2:3836242/1‑93 (MQ=255)

                                                                                  aGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTc       >  2:2627742/1‑93 (MQ=255)

                                                                                   gATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCt      <  2:580170/93‑1 (MQ=255)

                                                                                    aTCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTg     <  1:2359844/93‑1 (MQ=255)

                                                                                     tCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGa    >  2:3670611/1‑93 (MQ=255)

                                                                                     tCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGa    <  2:1121761/93‑1 (MQ=255)

                                                                                     tCCAGCAATTCGAGCGAATCAAAACAGCCTTCTTCCGACAACGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCTATTAATGTCTGa    >  1:5243042/1‑93 (MQ=255)

                                                                                       cAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGt  <  2:390825/93‑1 (MQ=255)

                                                                                       cAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGt  <  1:670344/93‑1 (MQ=255)

                                                                                       cAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGt  <  2:3036224/93‑1 (MQ=255)

TAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGT  >  NC_000913/1655155‑1655334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`