Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,662,601 T→C A27A (GCT→GCC)  b1591 → putative oxidoreductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,662,6010TC99.4% 547.8 / ‑5.8 171A27A (GCT→GCC) b1591putative oxidoreductase component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (89/80);  minor base G (0/1);  total (90/81)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 188 total aligned reads displayed.

ACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGC  >  NC_000913/1662511‑1662690
                                                                                          |                                                                                         
acaTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGcccc                                                                                         <  1:106063/93‑1 (MQ=255)
acaTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGcccc                                                                                         >  1:3367518/1‑93 (MQ=255)
acaTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGGGTTGGGCGCGCTGTTTTATTACGcccc                                                                                         >  2:4648311/1‑93 (MQ=255)
 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        <  2:4019947/93‑1 (MQ=255)
   tttCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTCTATTACGCCCCaga                                                                                      >  2:3743405/1‑93 (MQ=255)
    ttCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagag                                                                                     <  1:1705191/93‑1 (MQ=255)
    ttCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagag                                                                                     <  2:4068723/93‑1 (MQ=255)
     tCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagaga                                                                                    >  1:357087/1‑93 (MQ=255)
     tCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagaga                                                                                    <  1:4487636/93‑1 (MQ=255)
      cAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagagag                                                                                   <  2:6300038/93‑1 (MQ=255)
      cAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagagag                                                                                   >  1:6080811/1‑93 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGt                                                                                  <  1:3178581/93‑1 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGt                                                                                  <  2:6583578/93‑1 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCACCAGAGAGt                                                                                  >  1:854246/1‑93 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGGTTTATTACGCCCCAGAGAGt                                                                                  <  1:5442849/93‑1 (MQ=255)
        ggATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTg                                                                                 <  1:2240913/93‑1 (MQ=255)
         gATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCCGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGc                                                                                >  2:3564583/1‑93 (MQ=255)
          aTGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCg                                                                               <  1:2066652/93‑1 (MQ=255)
          aTGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCg                                                                               <  2:2456633/93‑1 (MQ=255)
            gTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGa                                                                             >  2:5359251/1‑93 (MQ=255)
             ttAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGaa                                                                            >  1:6124811/1‑93 (MQ=255)
               aGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGc                                                                          <  1:3590688/93‑1 (MQ=255)
                ggAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGcc                                                                         <  2:2672270/93‑1 (MQ=255)
                 gagtGACCCATTTTTCACAGCAAGATAATTTTCCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCg                                                                        <  1:3413542/90‑1 (MQ=255)
                    tGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCAc                                                                     <  1:4628272/93‑1 (MQ=255)
                     gACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCAcc                                                                    >  2:4231815/1‑93 (MQ=255)
                     gACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCAcc                                                                    >  2:4377033/1‑93 (MQ=255)
                     gACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCAcc                                                                    >  1:1565876/1‑93 (MQ=255)
                      aCCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACct                                                                   <  1:3706058/93‑1 (MQ=255)
                      aCCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACct                                                                   >  2:1132110/1‑93 (MQ=255)
                      aCCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACct                                                                   >  2:2718049/1‑93 (MQ=255)
                       cccATTTTTCTCAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctc                                                                  <  1:3460998/93‑1 (MQ=255)
                       cccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctc                                                                  >  1:535278/1‑93 (MQ=255)
                           tttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGt                                                              <  2:1828920/93‑1 (MQ=255)
                           tttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGt                                                              >  2:1366068/1‑93 (MQ=255)
                           tttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGt                                                              <  2:702571/93‑1 (MQ=255)
                           tttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCACCAGAGAGTGCGGAAGCCGCACCTCTGGt                                                              >  1:396278/1‑93 (MQ=255)
                           tttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCACTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGt                                                              <  2:5223365/93‑1 (MQ=255)
                                 acaGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGt                                                        >  2:4599761/1‑93 (MQ=255)
                                 acaGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGt                                                        >  1:4047721/1‑93 (MQ=255)
                                   aGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTAc                                                      <  2:3680808/93‑1 (MQ=255)
                                    gCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGACGCACCTCTGGTTGCGGTACt                                                     >  1:2998440/1‑93 (MQ=255)
                                       aGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCAc                                                  >  2:6716680/1‑93 (MQ=255)
                                       aGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCAc                                                  <  2:3337777/93‑1 (MQ=255)
                                       aGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCAc                                                  >  1:1746937/1‑93 (MQ=255)
                                       aGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCAc                                                  <  2:4965167/93‑1 (MQ=255)
                                       aGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCAc                                                  <  2:1333992/93‑1 (MQ=255)
                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCa                                                <  2:2400680/93‑1 (MQ=255)
                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCa                                                >  2:2200916/1‑93 (MQ=255)
                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCa                                                <  2:2193199/93‑1 (MQ=255)
                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCa                                                <  2:6632624/93‑1 (MQ=255)
                                          tAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAg                                               <  2:3163731/93‑1 (MQ=255)
                                          tAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAg                                               >  2:3236660/1‑93 (MQ=255)
                                          tAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAg                                               <  1:6362815/93‑1 (MQ=255)
                                          tAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGGTGCGGGGCTCACCAg                                               >  1:1243663/1‑93 (MQ=255)
                                            aTTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTg                                             <  2:1493226/93‑1 (MQ=255)
                                            aTTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTg                                             >  1:1883532/1‑93 (MQ=255)
                                             tttttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGa                                            <  2:6355155/93‑1 (MQ=255)
                                               tttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGGCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACg                                          >  2:4444810/1‑93 (MQ=255)
                                                   tGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTg                                      >  2:5478304/1‑93 (MQ=255)
                                                     tCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTggg                                    >  2:1025628/1‑93 (MQ=255)
                                                     tCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTggg                                    <  1:4836215/93‑1 (MQ=255)
                                                       gcggcgCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGaa                                  >  1:1146036/1‑93 (MQ=255)
                                                       gcggcgCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGaa                                  >  2:1465492/1‑93 (MQ=255)
                                                        cggcgCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGaaa                                 >  2:1853261/1‑93 (MQ=255)
                                                         ggcgCGCGTGTTGGGCGCTCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAAc                                <  2:6185108/93‑1 (MQ=255)
                                                              gcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt                           <  1:3924114/93‑1 (MQ=255)
                                                                 tgtgGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGc                        <  1:3311985/93‑1 (MQ=255)
                                                                  gtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGcc                       >  2:2296447/1‑93 (MQ=255)
                                                                   ttGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCct                      >  1:4786811/1‑93 (MQ=255)
                                                                    tGGGCGCGCTGTTTTATTACGCCCGAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAGCTCAGTGGCctc                     <  1:2908873/93‑1 (MQ=255)
                                                                    tGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCctc                     >  2:1688144/1‑93 (MQ=255)
                                                                     gggCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCctct                    >  1:1022482/1‑93 (MQ=255)
                                                                      ggCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTa                   >  1:2440550/1‑93 (MQ=255)
                                                                      ggCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTa                   >  1:1852640/1‑93 (MQ=255)
                                                                       gcgcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTAc                  >  2:4092260/1‑93 (MQ=255)
                                                                           gcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGTGAAACTCAGTGGCCTCTACCAGa              >  2:4138678/1‑93 (MQ=255)
                                                                           gcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGa              >  2:2475648/1‑93 (MQ=255)
                                                                           gcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGa              >  2:2036944/1‑93 (MQ=255)
                                                                             tGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAg            <  2:2758606/93‑1 (MQ=255)
                                                                             tGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAg            <  1:3536785/93‑1 (MQ=255)
                                                                             tGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAg            <  1:5359251/93‑1 (MQ=255)
                                                                             tGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAg            <  2:6165993/93‑1 (MQ=255)
                                                                              gTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGc           >  2:4123633/1‑93 (MQ=255)
                                                                              gTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGc           >  1:5228321/1‑93 (MQ=255)
                                                                              gTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGc           >  2:5259628/1‑93 (MQ=255)
                                                                               ttttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCg          >  1:2784947/1‑93 (MQ=255)
                                                                               ttttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCg          <  2:2151387/93‑1 (MQ=255)
                                                                               ttttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCg          >  1:3825998/1‑93 (MQ=255)
                                                                                   attaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt      <  1:1657358/93‑1 (MQ=255)
                                                                                   attaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt      >  1:4664811/1‑93 (MQ=255)
                                                                                    ttaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAtt     <  2:5234976/93‑1 (MQ=255)
                                                                                    ttaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAtt     >  2:1710429/1‑93 (MQ=255)
                                                                                    ttaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAtt     <  1:4856346/93‑1 (MQ=255)
                                                                                    ttaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAtt     <  2:2713356/93‑1 (MQ=255)
                                                                                     taCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTa    <  1:6585731/93‑1 (MQ=255)
                                                                                      aCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAg   <  2:4859313/93‑1 (MQ=255)
                                                                                       cGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAgc  <  1:5077898/93‑1 (MQ=255)
                                                                                       cGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAgc  <  1:5149086/93‑1 (MQ=255)
                                                                                       cGCCCCAGAGAGTGCGGAAGCCACACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAgc  <  2:5631533/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGC  >  NC_000913/1662511‑1662690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑