BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,693,549	A→G	W183R (TGG→CGG)	uidA ←	beta‑D‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,693,549	0	A	G	99.5%	649.3 / ‑4.7	205	W183R (TGG→CGG)	uidA	beta‑D‑glucuronidase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (83/121); total (84/121)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01

Only 100 of 219 total aligned reads displayed.

TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGT  >  NC_000913/1693459‑1693638

tGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCggg                                                                                         >  2:6438125/1‑93 (MQ=255)

tGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCggg                                                                                         <  2:1079075/93‑1 (MQ=255)

 gACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgt                                                                                        >  2:4249238/1‑93 (MQ=255)

  aCATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgtg                                                                                       <  1:1168782/93‑1 (MQ=255)

   cagcACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgtgt                                                                                      <  1:2851814/90‑1 (MQ=255)

   cATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgtgt                                                                                      >  2:6364556/1‑93 (MQ=255)

   cATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgtgt                                                                                      >  2:4066796/1‑93 (MQ=255)

    aTCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGtt                                                                                     <  2:1170883/93‑1 (MQ=255)

    aTCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGtt                                                                                     >  2:5474941/1‑93 (MQ=255)

    aTCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGtt                                                                                     <  2:3101482/93‑1 (MQ=255)

    aTCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGtt                                                                                     >  2:1778619/1‑93 (MQ=255)

     tCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTc                                                                                    >  1:1195020/1‑93 (MQ=255)

       aCCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCgg                                                                                  >  1:3619528/1‑93 (MQ=255)

        ccATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGc                                                                                 >  2:6708100/1‑93 (MQ=255)

          aTTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgt                                                                               <  1:5207127/93‑1 (MQ=255)

          aTTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgt                                                                               <  1:2932781/93‑1 (MQ=255)

           ttGCCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCCCCCGGGTGTTCGGCgtg                                                                              <  2:1496824/93‑1 (MQ=255)

              gCCACCCCCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgtggtg                                                                           <  2:801030/93‑1 (MQ=255)

              gCCACCACCTCCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgtggtg                                                                           <  2:5760560/93‑1 (MQ=255)

               ccaccaCCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGgtgt                                                                          <  1:1309884/93‑1 (MQ=255)

                caccccCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                         <  1:5291383/93‑1 (MQ=255)

                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                         >  2:6145649/1‑93 (MQ=255)

                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                       <  1:3487364/93‑1 (MQ=255)

                    accTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGc                                                                     >  1:3838214/1‑93 (MQ=255)

                    accTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGc                                                                     <  2:3666659/93‑1 (MQ=255)

                    accTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGc                                                                     >  1:4021679/1‑93 (MQ=255)

                     ccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCa                                                                    <  1:2558220/93‑1 (MQ=255)

                      cTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAt                                                                   <  1:166735/93‑1 (MQ=255)

                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                  <  1:6060195/93‑1 (MQ=255)

                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                  <  2:5947111/93‑1 (MQ=255)

                        gCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTa                                                                 <  1:6447135/93‑1 (MQ=255)

                         acAGTCAACAGACGCGTGGTTTCAGTCTTGCGCGACATGAGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTACAGCATTAc                                                                >  1:5519854/2‑93 (MQ=255)

                          cAGTCAACAGACGCGTGGTTACAGTCTTGGGCGACATGCGTCACCACGGTGATATAGTCCATCCGGGTGTTCGGCGTGGTGTAGAGCATTAcc                                                               >  2:124505/1‑92 (MQ=255)

                          cAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACg                                                               >  2:1656593/1‑93 (MQ=255)

                          cAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACg                                                               >  2:433185/1‑93 (MQ=255)

                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                              >  2:2389191/1‑93 (MQ=255)

                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                              <  1:5175222/93‑1 (MQ=255)

                            gTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCt                                                             >  2:6393202/1‑93 (MQ=255)

                              cAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGc                                                           <  1:1932435/93‑1 (MQ=255)

                              cAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGc                                                           <  2:5330934/93‑1 (MQ=255)

                                aCAGACGCATGGTTACAGTTTTGCGCGACATGCGTCACCACTGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGa                                                         >  1:6513780/1‑93 (MQ=255)

                                 cAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGAt                                                        >  1:6723381/1‑93 (MQ=255)

                                   gACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATgg                                                      >  1:4470973/1‑93 (MQ=255)

                                   gACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATgg                                                      >  1:4145977/1‑93 (MQ=255)

                                    aCGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGa                                                     <  2:2701603/93‑1 (MQ=255)

                                     cgcgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGAt                                                    <  2:5995561/93‑1 (MQ=255)

                                       cgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATcc                                                  <  1:3941012/93‑1 (MQ=255)

                                        gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATccc                                                 <  2:1340806/93‑1 (MQ=255)

                                         tGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCg                                                >  1:4873991/1‑93 (MQ=255)

                                          ggTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCACgg                                               >  1:1828787/1‑93 (MQ=255)

                                           gTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGc                                              >  1:3049288/1‑93 (MQ=255)

                                            ttACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCa                                             >  2:1866039/1‑93 (MQ=255)

                                                aGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGt                                         <  1:1971639/93‑1 (MQ=255)

                                                aGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGt                                         >  1:6638148/1‑93 (MQ=255)

                                                aGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGt                                         >  2:2512059/1‑93 (MQ=255)

                                                aGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGt                                         >  1:5143799/1‑93 (MQ=255)

                                                 gTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGtt                                        >  2:5316467/1‑93 (MQ=255)

                                                       cgcgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGaa                                  >  2:3118535/1‑93 (MQ=255)

                                                         cgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAAt                                <  2:5882023/93‑1 (MQ=255)

                                                         cgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAAt                                <  1:157971/93‑1 (MQ=255)

                                                         cgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAAt                                <  1:2475988/93‑1 (MQ=255)

                                                          gACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATc                               <  2:1513287/93‑1 (MQ=255)

                                                          gACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATc                               >  2:5158336/1‑93 (MQ=255)

                                                           aCATGCGTCACCACGGTGATATCGTTCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCa                              <  2:6479825/93‑1 (MQ=255)

                                                            cATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAt                             <  2:871425/93‑1 (MQ=255)

                                                             aTGCGTCAGCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATg                            <  1:3341875/93‑1 (MQ=255)

                                                             aTGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATg                            >  2:289125/1‑93 (MQ=255)

                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                           <  2:5086294/93‑1 (MQ=255)

                                                               gCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGa                          >  1:4248963/1‑93 (MQ=255)

                                                                 gTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAg                        <  2:2492980/93‑1 (MQ=255)

                                                                    accacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                     >  2:3250696/1‑93 (MQ=255)

                                                                     ccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAg                    >  2:843765/1‑93 (MQ=255)

                                                                     ccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAg                    <  1:5975343/93‑1 (MQ=255)

                                                                       acGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAc                  <  2:4018328/93‑1 (MQ=255)

                                                                       acGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAc                  <  2:1010266/93‑1 (MQ=255)

                                                                        cGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACt                 <  1:5419123/93‑1 (MQ=255)

                                                                          gTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGc               <  1:6113691/93‑1 (MQ=255)

                                                                          gTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGc               <  2:3407613/93‑1 (MQ=255)

                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt              <  1:3215542/93‑1 (MQ=255)

                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt              <  2:1615179/93‑1 (MQ=255)

                                                                            gATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtt             <  2:4852898/93‑1 (MQ=255)

                                                                             atatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCttt            <  1:373331/93‑1 (MQ=255)

                                                                              tatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtttt           <  2:6453072/93‑1 (MQ=255)

                                                                              tatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtttt           <  2:4654445/93‑1 (MQ=255)

                                                                              tatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtttt           >  1:18656/1‑93 (MQ=255)

                                                                              tatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtttt           <  1:5878983/93‑1 (MQ=255)

                                                                              tatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtttt           >  1:2683136/1‑93 (MQ=255)

                                                                              tatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTAAAAGAAATCATGGAAGTAAGACTGCtttt           <  2:5019479/93‑1 (MQ=255)

                                                                              tatCGTCCACCCGGGTGTTCGGCGGGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtttt           >  1:3262914/1‑93 (MQ=255)

                                                                               atCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCttttt          <  1:2087453/93‑1 (MQ=255)

                                                                                 cGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCt        <  2:6619565/93‑1 (MQ=255)

                                                                                 cGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCt        <  1:1047124/93‑1 (MQ=255)

                                                                                 cGTCCACCCCGGTGTTCGGAGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCt        <  1:886327/93‑1 (MQ=255)

                                                                                  gTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCtt       <  2:5575305/93‑1 (MQ=255)

                                                                                   tCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTg      <  1:2570486/93‑1 (MQ=255)

                                                                                   tCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTg      >  2:3553147/1‑93 (MQ=255)

                                                                                      ccccGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCg   <  2:1894989/92‑1 (MQ=255)

                                                                                      aCCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCg   >  1:246565/1‑93 (MQ=255)

                                                                                       cccGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGt  <  2:3763092/93‑1 (MQ=255)

                                                                                       cccGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGt  <  1:5726927/93‑1 (MQ=255)

TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGT  >  NC_000913/1693459‑1693638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`