BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,698,875	A→G	V499V (GTA→GTG)	malX →	MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,698,875	0	A	G	99.6%	776.8 / NA	234	V499V (GTA→GTG)	malX	MalX
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (134/99); minor base T (0/1); total (134/100)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCA  >  NC_000913/1698785‑1698964

 aaCTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCac                                                                                        >  2:5301473/1‑92 (MQ=255)

  aCTGCATTGCCCGTCTGTGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACTATCGGGGAATTGGCGTAGTGCAAc                                                                                       <  1:1868027/93‑1 (MQ=255)

  aCTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTANTGCaat                                                                                       >  2:1486180/1‑92 (MQ=255)

  aCTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGCCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAAc                                                                                       <  2:1642414/93‑1 (MQ=255)

   cTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACt                                                                                      <  1:1289884/93‑1 (MQ=255)

   cTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACt                                                                                      >  2:4796771/1‑93 (MQ=255)

      cATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTaa                                                                                   <  1:1654993/93‑1 (MQ=255)

      cATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTaa                                                                                   <  2:3289965/93‑1 (MQ=255)

      cATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTaa                                                                                   <  2:2832699/93‑1 (MQ=255)

       aTTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAAt                                                                                  >  2:1613863/1‑93 (MQ=255)

        ttACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATc                                                                                 <  1:1447680/93‑1 (MQ=255)

        ttACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATc                                                                                 >  1:2741014/1‑93 (MQ=255)

        ttACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATc                                                                                 <  1:5693199/93‑1 (MQ=255)

          aCCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCaa                                                                               >  1:6061174/1‑93 (MQ=255)

            ccGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACa                                                                             >  1:330928/1‑93 (MQ=255)

            ccGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACa                                                                             <  1:4927003/93‑1 (MQ=255)

              gTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATa                                                                           >  1:4754347/1‑93 (MQ=255)

               tCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATaa                                                                          >  2:137482/1‑93 (MQ=255)

               tCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATaa                                                                          >  2:1336420/1‑93 (MQ=255)

                 tGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATCAcc                                                                        >  1:3618704/1‑93 (MQ=255)

                 tGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAAcc                                                                        <  2:2564310/93‑1 (MQ=255)

                 tGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAAcc                                                                        >  2:386947/1‑93 (MQ=255)

                 tGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAAcc                                                                        <  1:757648/93‑1 (MQ=255)

                 tGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAAcc                                                                        >  1:514984/1‑93 (MQ=255)

                  gCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCt                                                                       >  1:2370060/1‑93 (MQ=255)

                  gCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCt                                                                       <  1:3449755/93‑1 (MQ=255)

                  gCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCt                                                                       <  1:2082584/93‑1 (MQ=255)

                     tttGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCa                                                                    >  2:417856/1‑93 (MQ=255)

                     tttGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCa                                                                    >  2:5847280/1‑93 (MQ=255)

                     tttGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCa                                                                    >  1:5047677/1‑93 (MQ=255)

                     tttGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCa                                                                    >  2:4472909/1‑93 (MQ=255)

                     tttGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCa                                                                    >  2:3604239/1‑93 (MQ=255)

                       tGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAgg                                                                  <  1:2444041/93‑1 (MQ=255)

                       tGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAgg                                                                  >  2:4893912/1‑93 (MQ=255)

                       tGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAgg                                                                  >  2:2316385/1‑93 (MQ=255)

                        gTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGgt                                                                 >  2:1240281/1‑93 (MQ=255)

                        gTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGgt                                                                 >  2:6339657/1‑93 (MQ=255)

                           tgtgAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGgttgt                                                              <  2:2033352/93‑1 (MQ=255)

                           tgtgAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGgttgt                                                              >  1:4682776/1‑93 (MQ=255)

                           tgtgAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGgttgt                                                              <  1:6123863/93‑1 (MQ=255)

                           tgagAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGgttgt                                                              >  1:4331766/4‑93 (MQ=255)

                            gtgAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGgttgtt                                                             >  2:316501/1‑93 (MQ=255)

                              gAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTAt                                                           >  2:3103141/1‑93 (MQ=255)

                               aaaGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTAtg                                                          >  1:2161069/1‑92 (MQ=255)

                               aaaGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATc                                                          <  1:2837648/93‑1 (MQ=255)

                                 aGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCgg                                                        >  2:4679351/1‑93 (MQ=255)

                                   atatGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGc                                                      >  1:5735774/1‑93 (MQ=255)

                                    tatGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGTCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGcc                                                     <  2:2497102/93‑1 (MQ=255)

                                    tatGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGcc                                                     >  2:5048227/1‑93 (MQ=255)

                                    tatGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGcc                                                     >  1:1339128/1‑93 (MQ=255)

                                      tctCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACAGAACCTGCAGGTTGTTATCGGGCcac                                                   >  2:6482954/3‑93 (MQ=255)

                                      tGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCcac                                                   >  2:764245/1‑93 (MQ=255)

                                      tGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCcac                                                   >  1:3179070/1‑93 (MQ=255)

                                      tGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCcac                                                   >  2:1497890/1‑93 (MQ=255)

                                        tCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACaa                                                 <  1:4854533/93‑1 (MQ=255)

                                          gCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGt                                               <  1:713730/93‑1 (MQ=255)

                                          gCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGt                                               <  1:5273120/93‑1 (MQ=255)

                                            ttGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtc                                             <  1:6195991/93‑1 (MQ=255)

                                             tGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtca                                            >  1:6471567/1‑93 (MQ=255)

                                              gTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtcag                                           >  2:6141333/1‑93 (MQ=255)

                                               ttAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtcagt                                          >  2:6260307/1‑93 (MQ=255)

                                                tAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtcagtc                                         <  1:2594286/93‑1 (MQ=255)

                                                tAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtcagtc                                         >  2:5564721/1‑93 (MQ=255)

                                                 aaTGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtcagtca                                        >  1:3041802/1‑93 (MQ=255)

                                                  aTGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtcagtcag                                       >  2:5786922/1‑93 (MQ=255)

                                                  aTGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtcagtcag                                       >  1:5570770/1‑93 (MQ=255)

                                                  aTGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTtcagtcag                                       >  1:5467942/1‑93 (MQ=255)

                                                   tgtgCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTcagtcagt                                      >  1:5282709/1‑93 (MQ=255)

                                                      gCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTaaa                                   >  1:401934/1‑93 (MQ=255)

                                                       cAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTaaaa                                  <  1:5603961/93‑1 (MQ=255)

                                                        aGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAg                                 <  1:992791/93‑1 (MQ=255)

                                                        aGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAg                                 >  1:1953679/1‑93 (MQ=255)

                                                        aGGCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAg                                 >  1:2277620/1‑93 (MQ=255)

                                                         ggCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGa                                <  1:1500775/93‑1 (MQ=255)

                                                          gCACTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGAt                               <  2:966339/93‑1 (MQ=255)

                                                             cTGAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGaa                            >  2:4304297/1‑93 (MQ=255)

                                                              tGAAGGACAATCGCGCAATTGGCGTAGTGCAACTTAATCAACATAGCCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGaaa                           <  2:6092100/93‑1 (MQ=255)

                                                               gAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAAt                          <  2:836877/93‑1 (MQ=255)

                                                               gAAGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAAt                          >  2:5403565/1‑93 (MQ=255)

                                                                aaGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATg                         >  2:5160776/1‑93 (MQ=255)

                                                                aaGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATg                         >  1:2988606/1‑93 (MQ=255)

                                                                aaGGACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATg                         >  1:6383205/1‑93 (MQ=255)

                                                                   gACAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGcc                      >  2:3077084/1‑93 (MQ=255)

                                                                     cAATCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCgg                    <  2:894659/93‑1 (MQ=255)

                                                                       aTCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTc                  >  1:1382929/1‑93 (MQ=255)

                                                                       aTCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTc                  <  1:6533258/93‑1 (MQ=255)

                                                                        tCGGGCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCt                 <  2:2945833/93‑1 (MQ=255)

                                                                          gggCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGa               >  1:5885053/1‑93 (MQ=255)

                                                                          gggCAATTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGAAAAAGATGAAATGGCCGGTCTGa               >  1:5210261/1‑93 (MQ=255)

                                                                             cAATGGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGc            <  2:6654474/93‑1 (MQ=255)

                                                                              aaTTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCa           >  1:1143208/1‑93 (MQ=255)

                                                                              aaTTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCa           >  1:4386015/1‑93 (MQ=255)

                                                                              aaTTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCa           >  1:507974/1‑93 (MQ=255)

                                                                               aTTGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCTACAAGCTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCAt          >  2:4123451/1‑93 (MQ=255)

                                                                                 tGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATAc        <  1:3736431/93‑1 (MQ=255)

                                                                                 tGGCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATAc        <  2:5656852/93‑1 (MQ=255)

                                                                                  ggCGTAGTGCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACt       <  2:1534698/93‑1 (MQ=255)

                                                                                     gtagtgCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTc    <  2:2281513/93‑1 (MQ=255)

                                                                                      tagtgCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTcc   <  1:815520/93‑1 (MQ=255)

                                                                                      tagtgCAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTcc   <  2:3664773/93‑1 (MQ=255)

TAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGGACAATCGGGCAATTGGCGTAGTACAACTTAATCAACATAACCTGCAGGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCA  >  NC_000913/1698785‑1698964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`