BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,714,480	A→G	F256S (TTC→TCC)	tyrS ←	tyrosine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,480	0	A	G	99.6%	740.5 / ‑4.6	226	F256S (TTC→TCC)	tyrS	tyrosine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (116/109); minor base T (1/0); total (117/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 231 total aligned reads displayed.

AGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAG  >  NC_000913/1714390‑1714568

aGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAt                                                                                        >  1:1751501/1‑93 (MQ=255)

 gggCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGAtt                                                                                       >  1:4793542/1‑93 (MQ=255)

   gCGTTGATCTCTTCAATACTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTg                                                                                     <  2:3406358/93‑1 (MQ=255)

    cattGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGt                                                                                    <  1:3861140/91‑1 (MQ=255)

     gTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTa                                                                                   <  2:1275891/93‑1 (MQ=255)

      ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGTAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTAc                                                                                  >  2:4821311/1‑93 (MQ=255)

      ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTAc                                                                                  >  1:4869398/1‑93 (MQ=255)

      ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTAc                                                                                  <  2:3605914/93‑1 (MQ=255)

        gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACgg                                                                                <  2:6139992/93‑1 (MQ=255)

        gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACgg                                                                                >  2:222022/1‑93 (MQ=255)

        gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACgg                                                                                >  1:2196665/1‑93 (MQ=255)

        gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACgg                                                                                >  2:5587014/1‑93 (MQ=255)

         aTCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACggt                                                                               >  2:282064/1‑92 (MQ=255)

          tctcTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGc                                                                              >  2:3160035/1‑93 (MQ=255)

          tctcTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGc                                                                              <  2:5111709/93‑1 (MQ=255)

          tctcTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGc                                                                              <  1:4351767/93‑1 (MQ=255)

           ctctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCt                                                                             >  1:6642764/1‑93 (MQ=255)

            tctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTg                                                                            >  2:3725469/1‑93 (MQ=255)

             ctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTgg                                                                           <  1:5503895/93‑1 (MQ=255)

              ttCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGt                                                                          <  2:439185/93‑1 (MQ=255)

              ttCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGt                                                                          <  2:719497/93‑1 (MQ=255)

              ttCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGt                                                                          >  1:2384202/1‑93 (MQ=255)

               tCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtt                                                                         >  2:5701355/1‑93 (MQ=255)

               tCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtt                                                                         >  1:6564465/1‑93 (MQ=255)

                 aaTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtttt                                                                       <  2:1619736/93‑1 (MQ=255)

                 aaTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtttt                                                                       <  2:3869052/93‑1 (MQ=255)

                 aaTGCTCATAAAGAAGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGtttt                                                                       <  1:5122486/93‑1 (MQ=255)

                       cATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCgg                                                                 >  2:5038134/1‑93 (MQ=255)

                        aTAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGa                                                                <  2:3789022/93‑1 (MQ=255)

                         tAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGAt                                                               >  1:240479/1‑93 (MQ=255)

                         tAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGAt                                                               >  1:3947234/1‑93 (MQ=255)

                          aaaGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATc                                                              <  1:5652846/93‑1 (MQ=255)

                           aaGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATcc                                                             >  1:2982899/1‑93 (MQ=255)

                            aGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCa                                                            <  1:5391704/93‑1 (MQ=255)

                             ggTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCaa                                                           >  2:2031582/1‑93 (MQ=255)

                               tGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAAcc                                                         >  1:4475645/1‑93 (MQ=255)

                               tGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAAcc                                                         >  2:6424237/1‑93 (MQ=255)

                                gaagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCa                                                        <  1:5099540/93‑1 (MQ=255)

                                 aagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGCACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAg                                                       >  1:1025373/1‑93 (MQ=255)

                                 aagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAg                                                       >  1:6445945/1‑93 (MQ=255)

                                   gaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGAc                                                     <  2:1687310/93‑1 (MQ=255)

                                   gaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGAc                                                     >  2:4433773/1‑93 (MQ=255)

                                   gaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGAc                                                     <  2:662419/93‑1 (MQ=255)

                                    aaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACt                                                    >  1:6299811/1‑93 (MQ=255)

                                    aaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACt                                                    <  2:2192142/93‑1 (MQ=255)

                                     aCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                                   <  1:803899/93‑1 (MQ=255)

                                     aCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                                   >  1:5935666/1‑93 (MQ=255)

                                     aCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                                   >  1:4646902/1‑93 (MQ=255)

                                     aCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                                   >  2:4170435/1‑93 (MQ=255)

                                      cTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgc                                                  <  2:2988105/93‑1 (MQ=255)

                                        tCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgcgc                                                <  2:5667631/93‑1 (MQ=255)

                                         cAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgcc                                               >  1:3235539/1‑93 (MQ=255)

                                          aGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgccg                                              <  2:1010767/93‑1 (MQ=255)

                                          aGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgccg                                              >  1:6712468/1‑93 (MQ=255)

                                            gAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCgccgcc                                            >  1:6625912/1‑93 (MQ=255)

                                            gAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCgccgcc                                            <  1:2600628/93‑1 (MQ=255)

                                             aaGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCt                                           <  2:4252322/93‑1 (MQ=255)

                                             aaGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCt                                           >  1:6039472/1‑93 (MQ=255)

                                              aGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCtt                                          >  1:4560409/1‑93 (MQ=255)

                                              aGCGGTAAACGTCGGCATCCGCAGTGTTGATCAAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCtt                                          <  1:1081230/93‑1 (MQ=255)

                                               gCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTc                                         <  1:424224/93‑1 (MQ=255)

                                                  gTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGt                                      <  1:6087653/93‑1 (MQ=255)

                                                    aagcGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGttt                                    <  1:1392568/90‑1 (MQ=255)

                                                    aaaCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGttt                                    >  2:5788348/1‑93 (MQ=255)

                                                     aaCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtttt                                   >  2:1766054/1‑93 (MQ=255)

                                                     aaCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtttt                                   <  2:6005126/93‑1 (MQ=255)

                                                      aCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTa                                  >  1:432587/1‑93 (MQ=255)

                                                      aCGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTa                                  >  2:2400119/1‑93 (MQ=255)

                                                        gTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTAcc                                >  2:2145674/1‑93 (MQ=255)

                                                         tCGGCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCa                               >  2:4521067/1‑93 (MQ=255)

                                                            gCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTATCAAAt                            >  1:932053/1‑93 (MQ=255)

                                                            gCATCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAt                            >  1:1853157/1‑93 (MQ=255)

                                                               tCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTg                         >  1:2042057/1‑93 (MQ=255)

                                                               tCCGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTg                         <  1:5601319/93‑1 (MQ=255)

                                                                 cGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTggg                       >  1:4998243/1‑92 (MQ=255)

                                                                 cGCAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGt                       <  2:3983327/93‑1 (MQ=255)

                                                                   cAGTGTTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGc                     <  2:5914718/93‑1 (MQ=255)

                                                                     gtgtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                   >  2:4901910/1‑93 (MQ=255)

                                                                     gtgtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa                   >  1:2000767/1‑93 (MQ=255)

                                                                       gtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                 >  1:1229662/1‑93 (MQ=255)

                                                                       gtTGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                 >  1:1714396/1‑93 (MQ=255)

                                                                        ttGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCt                >  1:1315682/1‑93 (MQ=255)

                                                                        ttGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCt                >  1:3170491/1‑93 (MQ=255)

                                                                         tGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTg               <  2:3963019/93‑1 (MQ=255)

                                                                         tGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTg               <  1:148244/93‑1 (MQ=255)

                                                                         tGATCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTg               >  2:2507349/1‑93 (MQ=255)

                                                                           aTCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCt             >  1:3621158/1‑93 (MQ=255)

                                                                           aTCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCt             >  1:2745495/1‑93 (MQ=255)

                                                                            tCCAGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCtt            <  2:3038025/93‑1 (MQ=255)

                                                                               aGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAg         <  2:4450389/93‑1 (MQ=255)

                                                                               aGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAg         <  1:2486137/93‑1 (MQ=255)

                                                                               aGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAg         >  1:2805321/1‑93 (MQ=255)

                                                                               aGAACTGGTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAg         <  1:4047089/93‑1 (MQ=255)

                                                                                     ggTAGGATTTGTCCGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCa   <  2:1496238/93‑1 (MQ=255)

                                                                                     ggTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGGGCCGCCTTCAGTTATACCAAATTTGGTGCCATCTGCTTTAGTGATCa   >  1:6181619/1‑93 (MQ=255)

                                                                                     ggTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCa   <  2:460791/93‑1 (MQ=255)

                                                                                     ggTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCa   <  1:1521390/93‑1 (MQ=255)

                                                                                      gTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAg  <  2:4119525/93‑1 (MQ=255)

                                                                                      gTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAg  <  2:1812480/93‑1 (MQ=255)

                                                                                      gTAGGATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAg  >  1:4218134/1‑93 (MQ=255)

AGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAG  >  NC_000913/1714390‑1714568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`