BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	195,014	A→G	I38V (ATT→GTT)	yaeS →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	195,014	0	A	G	99.6%	870.0 / NA	269	I38V (ATT→GTT)	yaeS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (140/128); total (141/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

CCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTG  >  NC_000913/194924‑195103

 cACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTc                                                                                        >  1:4817236/1‑93 (MQ=255)

 cACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTc                                                                                        <  2:509174/93‑1 (MQ=255)

  aCTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCg                                                                                       >  1:1849015/1‑93 (MQ=255)

   cTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGt                                                                                      <  1:764934/93‑1 (MQ=255)

   cTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGt                                                                                      >  1:5047103/1‑93 (MQ=255)

    ttAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTg                                                                                     >  2:1207116/1‑93 (MQ=255)

      aGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGcc                                                                                   <  2:5800812/93‑1 (MQ=255)

      aGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGcc                                                                                   <  2:218009/93‑1 (MQ=255)

      aGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGcc                                                                                   <  2:2250513/93‑1 (MQ=255)

       gCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCt                                                                                  <  2:691318/93‑1 (MQ=255)

       gCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCt                                                                                  <  1:3570073/93‑1 (MQ=255)

        cGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCTATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCtt                                                                                 <  1:2647544/93‑1 (MQ=255)

         gAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCttt                                                                                >  1:4352574/1‑93 (MQ=255)

           aaaaTTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTgg                                                                              <  2:1220890/93‑1 (MQ=255)

            aaaTTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTggg                                                                             >  2:1437900/1‑93 (MQ=255)

              aTTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCa                                                                           >  2:3288094/1‑93 (MQ=255)

              aTTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCa                                                                           <  2:3690450/93‑1 (MQ=255)

              aTTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCa                                                                           <  2:667150/93‑1 (MQ=255)

               ttGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCAt                                                                          >  2:444080/1‑93 (MQ=255)

                  ccAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATaaa                                                                       >  1:5694300/1‑93 (MQ=255)

                  ccAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATaaa                                                                       <  2:3543875/93‑1 (MQ=255)

                   cAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAg                                                                      <  1:1603504/93‑1 (MQ=255)

                   cAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAg                                                                      <  2:5516922/93‑1 (MQ=255)

                   cAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAg                                                                      >  1:3351014/1‑93 (MQ=255)

                    aGCGCATGTCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGc                                                                     <  1:3712045/93‑1 (MQ=255)

                     gcgcATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGAGCCTTTGGGCATAAAGcc                                                                    >  1:4410294/1‑93 (MQ=255)

                      cgcATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCg                                                                   <  1:4869493/93‑1 (MQ=255)

                      cgcATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCg                                                                   <  2:5615383/93‑1 (MQ=255)

                       gcATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCgg                                                                  <  2:4204216/93‑1 (MQ=255)

                        cATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCggg                                                                 <  2:3634953/93‑1 (MQ=255)

                        cATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCggg                                                                 >  1:2028493/1‑93 (MQ=255)

                        cATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCggg                                                                 <  1:4212628/93‑1 (MQ=255)

                          tGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGc                                                               >  1:462861/1‑93 (MQ=255)

                          tGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGc                                                               >  1:4147808/1‑93 (MQ=255)

                          tGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGc                                                               >  2:2616365/1‑93 (MQ=255)

                           ggCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCa                                                              >  1:2148104/1‑93 (MQ=255)

                            gCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCaa                                                             <  1:3077408/93‑1 (MQ=255)

                             cTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCaaa                                                            >  2:2066138/1‑93 (MQ=255)

                              tGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCaaaa                                                           >  1:2597514/1‑93 (MQ=255)

                              tGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCaaaa                                                           <  2:6548050/93‑1 (MQ=255)

                              tGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCaaaa                                                           <  1:5342421/93‑1 (MQ=255)

                              tGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCaaaa                                                           >  1:1743771/1‑93 (MQ=255)

                                ccGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtc                                                         >  1:6656933/1‑93 (MQ=255)

                                 cGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtcc                                                        >  2:4762742/1‑93 (MQ=255)

                                    cATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtc                                                     <  1:6618317/93‑1 (MQ=255)

                                    cATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAAtccgtc                                                     <  2:1568512/93‑1 (MQ=255)

                                       gTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTccgc                                                  >  2:3950662/1‑93 (MQ=255)

                                        ttGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTccgcc                                                 >  1:564304/1‑93 (MQ=255)

                                          gCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCgg                                               >  1:6700630/1‑93 (MQ=255)

                                          gCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCgg                                               >  2:6241767/1‑93 (MQ=255)

                                           cGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCggg                                              >  2:4234581/1‑93 (MQ=255)

                                             aTCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCt                                            >  1:3284207/1‑93 (MQ=255)

                                             aTCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCt                                            >  2:3120597/1‑93 (MQ=255)

                                                aTTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGtc                                         >  2:5193430/1‑93 (MQ=255)

                                                aTTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGtc                                         >  2:496278/1‑93 (MQ=255)

                                                   aTGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTctct                                      >  1:2103024/1‑93 (MQ=255)

                                                    tGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCtt                                     <  1:4504741/93‑1 (MQ=255)

                                                     ggACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCttt                                    >  1:3108170/1‑93 (MQ=255)

                                                     ggACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCttt                                    <  2:1480717/93‑1 (MQ=255)

                                                     ggACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCttt                                    >  1:4132221/1‑93 (MQ=255)

                                                     ggACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCttt                                    <  2:2365212/93‑1 (MQ=255)

                                                      gACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCtttt                                   <  2:4802574/93‑1 (MQ=255)

                                                      gACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCtttt                                   <  2:3784997/93‑1 (MQ=255)

                                                      gACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCtttt                                   <  1:40307/93‑1 (MQ=255)

                                                       aCGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTg                                  <  1:6243121/93‑1 (MQ=255)

                                                        cGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGc                                 <  1:2921819/93‑1 (MQ=255)

                                                        cGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGc                                 <  1:1121930/93‑1 (MQ=255)

                                                        cGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGc                                 <  2:6012895/93‑1 (MQ=255)

                                                           cAATGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGc                              <  1:6463085/93‑1 (MQ=255)

                                                            aaTGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGcc                             >  2:2687503/1‑93 (MQ=255)

                                                            aaTGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGcc                             >  2:1365447/1‑93 (MQ=255)

                                                            aaTGACGGCTAGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGccgac                             <  1:5928896/93‑5 (MQ=255)

                                                             aTGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCca                            >  2:5831259/1‑93 (MQ=255)

                                                             aTGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCca                            >  1:6578659/1‑93 (MQ=255)

                                                             aTGGCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCca                            >  2:1622885/1‑93 (MQ=255)

                                                               ggCCGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCcaac                          <  2:5687701/93‑1 (MQ=255)

                                                                 ccGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCcaacaa                        <  1:2803267/93‑1 (MQ=255)

                                                                 ccGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCcaacaa                        >  1:4709701/1‑93 (MQ=255)

                                                                  cGCTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCaacaac                       <  1:6109431/93‑1 (MQ=255)

                                                                    cTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACgg                     <  2:4156346/93‑1 (MQ=255)

                                                                    cTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACgg                     >  1:3529890/1‑93 (MQ=255)

                                                                    cTGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACgg                     <  1:942620/93‑1 (MQ=255)

                                                                     tGGGCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGt                    >  2:1745927/1‑93 (MQ=255)

                                                                       ggCAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTAt                  <  1:4377244/93‑1 (MQ=255)

                                                                         cAAAAAAGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTg                >  1:2845674/1‑93 (MQ=255)

                                                                          aaaaaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAACATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGa               <  1:5415139/93‑1 (MQ=255)

                                                                          aaaaaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGa               >  2:5316996/1‑93 (MQ=255)

                                                                          aaaaaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGa               >  1:1321449/1‑93 (MQ=255)

                                                                          aaaaaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGa               >  1:4702265/1‑93 (MQ=255)

                                                                            aaaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAgg             >  1:3229610/1‑93 (MQ=255)

                                                                            aaaaGCAAGGGAAGGTTCGTGCCGTTGGGCATAAAGCCGGTGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAgg             <  1:6309064/93‑1 (MQ=255)

                                                                             aaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGc            >  1:2353265/1‑93 (MQ=255)

                                                                             aaaGCAAGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGc            <  1:5202319/93‑1 (MQ=255)

                                                                                   aGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAAc      <  2:2827541/93‑1 (MQ=255)

                                                                                   aGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAAc      >  2:1914201/1‑93 (MQ=255)

                                                                                   aGGGAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAAc      <  2:1045896/93‑1 (MQ=255)

                                                                                    gggAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACg     >  1:1882282/1‑93 (MQ=255)

                                                                                    gggAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACg     >  1:1439093/1‑93 (MQ=255)

                                                                                     ggAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGc    <  2:5035138/93‑1 (MQ=255)

                                                                                     ggAAGGTTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGc    <  2:29442/93‑1 (MQ=255)

CCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCATGTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCCTTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAACGGTATTGAGGCGTTAACGCTG  >  NC_000913/194924‑195103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`