BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,753,749	A→G	I10V (ATC→GTC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,753,749	0	A	G	100.0%	931.1 / NA	283	I10V (ATC→GTC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (139/144); total (139/144)

Only 100 of 287 total aligned reads displayed.

TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC  >  NC_000913/1753659‑1753838

 ccGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCg                                                                                        >  2:4806865/1‑93 (MQ=255)

  cGGCAGTGCGCCCAGAAAGTAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCgg                                                                                       >  1:1537810/1‑93 (MQ=255)

   ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGa                                                                                      >  1:3811509/1‑93 (MQ=255)

   ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGa                                                                                      >  2:2365477/1‑93 (MQ=255)

    gCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAc                                                                                     <  1:4089650/93‑1 (MQ=255)

    gCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAc                                                                                     <  2:2206529/93‑1 (MQ=255)

     cAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAcc                                                                                    <  1:5670660/93‑1 (MQ=255)

      tgTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCg                                                                                   >  2:6670669/2‑93 (MQ=255)

          cgcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGaaaa                                                                               >  2:2614211/1‑93 (MQ=255)

           gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAc                                                                              <  2:518757/93‑1 (MQ=255)

           gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAc                                                                              <  1:6710762/93‑1 (MQ=255)

           gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAc                                                                              <  1:2993200/93‑1 (MQ=255)

            cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAcc                                                                             <  2:4780898/93‑1 (MQ=255)

             ccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCg                                                                            <  1:1024390/93‑1 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  2:6184883/1‑93 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  2:5649739/1‑93 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  2:5623556/93‑1 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  1:183236/1‑93 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  2:2128480/1‑93 (MQ=255)

                gAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAt                                                                         <  2:6447374/93‑1 (MQ=255)

                gAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAt                                                                         >  2:4152565/1‑93 (MQ=255)

                  aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCt                                                                       <  1:5640086/93‑1 (MQ=255)

                    gCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGa                                                                     >  1:110384/1‑93 (MQ=255)

                      aaGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAag                                                                   >  1:3742328/1‑93 (MQ=255)

                       aGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAaga                                                                  <  1:2389986/93‑1 (MQ=255)

                        gTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAagag                                                                 >  1:3017150/1‑93 (MQ=255)

                         tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAgaga                                                                >  2:2782571/1‑93 (MQ=255)

                         tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAgaga                                                                <  2:4049832/93‑1 (MQ=255)

                         tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAgaga                                                                <  2:2276805/93‑1 (MQ=255)

                         tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAgaga                                                                <  1:4705224/93‑1 (MQ=255)

                         tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAgaga                                                                >  1:6499434/1‑93 (MQ=255)

                         tttCTCCCATCCGTCTCAACTTAAAGCCTAAGACTGTCATGAAAAAGACCAAAATGGTTTGCCCCGTCGGACCGAAAACCGAATCTGAAgaga                                                                <  2:3139206/93‑1 (MQ=255)

                          ttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGAt                                                               <  1:276124/93‑1 (MQ=255)

                            ctcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGt                                                             <  2:4430037/93‑1 (MQ=255)

                            ctcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGt                                                             <  2:854689/93‑1 (MQ=255)

                              cccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTa                                                           <  2:1563827/93‑1 (MQ=255)

                              cccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAACGAATCTGAGGAGATGtca                                                           >  2:3771904/1‑91 (MQ=255)

                               ccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAg                                                          >  1:4914731/1‑93 (MQ=255)

                                cATCCTTCTCAACTTAAAGACTAAGACTGTCGTGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGc                                                         >  2:1887906/1‑93 (MQ=255)

                                cATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGc                                                         <  2:1969354/93‑1 (MQ=255)

                                   ccTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaa                                                      <  1:3429560/93‑1 (MQ=255)

                                   ccTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaa                                                      <  1:1335140/93‑1 (MQ=255)

                                     ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                    <  1:3200268/93‑1 (MQ=255)

                                     ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                    >  2:4292256/1‑93 (MQ=255)

                                     ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                    <  2:1486037/93‑1 (MQ=255)

                                      tctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                                   <  2:5043776/93‑1 (MQ=255)

                                      tctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                                   <  1:4600211/93‑1 (MQ=255)

                                       ctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATg                                                  <  1:5171156/93‑1 (MQ=255)

                                        tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGc                                                 <  1:5801948/93‑1 (MQ=255)

                                         cAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                                                >  2:5785856/1‑93 (MQ=255)

                                          aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTg                                               <  2:5770696/93‑1 (MQ=255)

                                           aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTgg                                              >  2:3475384/1‑93 (MQ=255)

                                           aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTgg                                              <  1:4033700/93‑1 (MQ=255)

                                           aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTgg                                              >  2:1104633/1‑93 (MQ=255)

                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             <  1:6175057/93‑1 (MQ=255)

                                             ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAc                                            >  1:1243361/1‑93 (MQ=255)

                                             ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAc                                            >  1:5032317/1‑93 (MQ=255)

                                               aaaGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                          >  1:657774/1‑93 (MQ=255)

                                               aaaGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                          >  1:4993845/1‑93 (MQ=255)

                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                                         <  2:3943760/93‑1 (MQ=255)

                                                 agactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAACCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                                        <  1:594347/93‑1 (MQ=255)

                                                 agactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                                        >  2:745283/1‑93 (MQ=255)

                                                  gactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                                       >  1:775431/1‑93 (MQ=255)

                                                       agactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACTGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGa                                  >  2:3246232/1‑93 (MQ=255)

                                                         actGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc                                <  2:6491174/93‑1 (MQ=255)

                                                         actGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc                                >  1:68046/1‑93 (MQ=255)

                                                          ctGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg                               >  2:1661621/1‑93 (MQ=255)

                                                          ctGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg                               <  2:4582073/93‑1 (MQ=255)

                                                           tGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGt                              >  2:1804822/1‑93 (MQ=255)

                                                           tGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGt                              >  1:2030150/1‑93 (MQ=255)

                                                            gTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt                             >  2:1341478/1‑93 (MQ=255)

                                                            gTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt                             <  1:2329077/93‑1 (MQ=255)

                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            >  1:3567038/1‑93 (MQ=255)

                                                             tCATGAAAAAGACCAAAATAGTTCGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            <  1:6343597/93‑1 (MQ=255)

                                                              cATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt                           <  1:731158/93‑1 (MQ=255)

                                                               aTGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATg                          >  2:527249/1‑93 (MQ=255)

                                                                tGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGc                         >  1:999044/1‑93 (MQ=255)

                                                                  aaaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGt                       >  1:435607/1‑93 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      >  1:2206454/1‑93 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      <  2:5378926/93‑1 (MQ=255)

                                                                    aaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCt                     <  2:6235650/93‑1 (MQ=255)

                                                                     aaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTg                    >  2:2841446/1‑93 (MQ=255)

                                                                       gACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                  >  1:5383390/1‑93 (MQ=255)

                                                                          cAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACtt               >  1:4285595/1‑93 (MQ=255)

                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              <  1:2649443/93‑1 (MQ=255)

                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              >  1:2111844/1‑93 (MQ=255)

                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              <  2:1525599/93‑1 (MQ=255)

                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              <  1:1667623/93‑1 (MQ=255)

                                                                            aaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtct             <  1:6060044/93‑1 (MQ=255)

                                                                            aaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtct             <  2:5978128/93‑1 (MQ=255)

                                                                             aaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctc            <  1:6427335/93‑1 (MQ=255)

                                                                               ttGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctctc          >  2:2635285/1‑93 (MQ=255)

                                                                               ttGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctctc          <  2:6364137/93‑1 (MQ=255)

                                                                                 gTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAt        >  2:3641330/1‑93 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       >  1:2515453/1‑93 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       >  2:2958303/1‑93 (MQ=255)

                                                                                    tGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGt     <  1:3266747/93‑1 (MQ=255)

                                                                                    tGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGt     <  2:6073229/93‑1 (MQ=255)

                                                                                    tGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGt     >  1:4477369/1‑93 (MQ=255)

                                                                                     gCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTg    >  2:4674009/1‑93 (MQ=255)

TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC  >  NC_000913/1753659‑1753838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`