BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,008	T→C	Y264Y (TAT→TAC)	b1695 →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,008	0	T	C	99.6%	795.6 / ‑4.8	245	Y264Y (TAT→TAC)	b1695	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (116/128); total (116/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 254 total aligned reads displayed.

TGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAA  >  NC_000913/1775918‑1776097

 gtgATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGcc                                                                                        >  1:5871827/1‑93 (MQ=255)

 gtgATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGcc                                                                                        >  1:3476983/1‑93 (MQ=255)

  tgATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGcca                                                                                       >  1:4039898/1‑93 (MQ=255)

  tgATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGcca                                                                                       >  2:4293854/1‑93 (MQ=255)

   gATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGccaa                                                                                      >  2:2097240/1‑93 (MQ=255)

   gATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGccaa                                                                                      >  2:3677927/1‑93 (MQ=255)

    aTGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGccaac                                                                                     >  1:1745119/1‑93 (MQ=255)

    aTGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGccaac                                                                                     <  1:4442887/93‑1 (MQ=255)

    aTGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGccaac                                                                                     <  2:4637010/93‑1 (MQ=255)

       tACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGccaaccaa                                                                                  <  1:3115929/93‑1 (MQ=255)

        aCAACTTTGAGATGGAGCGCGTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCcaaccaac                                                                                 <  2:4439925/93‑1 (MQ=255)

        aCAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCcaaccaac                                                                                 <  2:6389476/93‑1 (MQ=255)

        aCAACTTTGAGATGGAGCGCCTGACCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCcaacaaac                                                                                 >  1:846928/1‑93 (MQ=255)

         cAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACg                                                                                >  2:2624339/1‑93 (MQ=255)

          aaCTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGt                                                                               <  1:3707553/93‑1 (MQ=255)

            cTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTAt                                                                             >  1:5387103/1‑93 (MQ=255)

            cTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTAt                                                                             <  2:2740768/93‑1 (MQ=255)

              ttGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCg                                                                           >  1:3365377/1‑93 (MQ=255)

                gagaTGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCt                                                                         <  1:6103007/93‑1 (MQ=255)

                 agaTGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCtt                                                                        <  1:4360241/93‑1 (MQ=255)

                 agaTGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCtt                                                                        <  2:3074712/93‑1 (MQ=255)

                   aTGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCtttt                                                                      >  1:2552069/1‑93 (MQ=255)

                   aTGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCtttt                                                                      <  2:6176965/93‑1 (MQ=255)

                    tGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTg                                                                     <  1:3594481/93‑1 (MQ=255)

                     ggAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTgg                                                                    >  1:1232789/1‑93 (MQ=255)

                     ggAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTgg                                                                    >  2:5200421/1‑93 (MQ=255)

                       aGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTa                                                                  <  2:140563/93‑1 (MQ=255)

                        gcgcCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTaa                                                                 <  2:3588069/93‑1 (MQ=255)

                        gcgcCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTaa                                                                 <  2:4932653/93‑1 (MQ=255)

                          gcCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGc                                                               >  2:2307350/1‑93 (MQ=255)

                           ccTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGcc                                                              >  1:975496/1‑93 (MQ=255)

                            cTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGccc                                                             >  1:1075994/1‑93 (MQ=255)

                             tGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCa                                                            >  1:4821732/1‑93 (MQ=255)

                             tGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCa                                                            >  1:4858510/1‑93 (MQ=255)

                              gATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCAt                                                           <  1:2689915/93‑1 (MQ=255)

                              gATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCAt                                                           >  1:631727/1‑93 (MQ=255)

                               aTCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCAtt                                                          <  2:3276797/93‑1 (MQ=255)

                                tCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTg                                                         >  2:6087146/1‑93 (MQ=255)

                                tCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTg                                                         >  2:5401644/1‑93 (MQ=255)

                                  aaCGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGt                                                       >  1:4484398/1‑93 (MQ=255)

                                  aaCGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGt                                                       <  2:615521/93‑1 (MQ=255)

                                   aCGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTc                                                      >  2:6672329/1‑93 (MQ=255)

                                   aCGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTc                                                      <  2:744814/93‑1 (MQ=255)

                                    cgccgcGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCa                                                     <  1:3563005/93‑1 (MQ=255)

                                      ccgcGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATa                                                   >  2:1546992/1‑93 (MQ=255)

                                         cgcgcAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAAcc                                                >  1:4374512/1‑93 (MQ=255)

                                          gcgcAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCa                                               <  2:6527167/93‑1 (MQ=255)

                                           cgcAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAg                                              <  1:5617520/93‑1 (MQ=255)

                                            gcagcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAga                                             >  1:619618/1‑93 (MQ=255)

                                            gcagcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAga                                             >  2:6631789/1‑93 (MQ=255)

                                            gcagcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAga                                             <  1:2812339/93‑1 (MQ=255)

                                             cagcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgat                                            <  2:6005458/93‑1 (MQ=255)

                                             cagcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgat                                            <  2:1475660/93‑1 (MQ=255)

                                              agcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgatc                                           <  1:6436585/93‑2 (MQ=255)

                                               gcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgatga                                          >  2:1114384/1‑93 (MQ=255)

                                               gcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgatga                                          <  1:2219517/93‑1 (MQ=255)

                                               gcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgatga                                          >  2:4540901/1‑93 (MQ=255)

                                               gcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgatga                                          <  1:3846996/93‑1 (MQ=255)

                                                caCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgatgat                                         >  1:4004633/1‑93 (MQ=255)

                                                caCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgatgat                                         >  2:6415576/1‑93 (MQ=255)

                                                  ccGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATcc                                       <  1:5071652/93‑1 (MQ=255)

                                                  ccGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATcc                                       >  2:5728090/1‑93 (MQ=255)

                                                    ggCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAg                                     <  2:4162832/93‑1 (MQ=255)

                                                    cgCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAg                                     <  2:712616/92‑1 (MQ=255)

                                                       tttGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGaa                                  >  2:1211340/1‑93 (MQ=255)

                                                       tttGCCGAATGCGCCTTTGAAGATACCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGaa                                  >  1:2094885/1‑93 (MQ=255)

                                                        ttGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGaaa                                 <  1:1946216/93‑1 (MQ=255)

                                                         tGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGaaaa                                >  2:4826148/1‑93 (MQ=255)

                                                         tGCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGaaaa                                >  1:287159/1‑93 (MQ=255)

                                                          gCCGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGaaaaa                               >  1:6636797/1‑93 (MQ=255)

                                                           ccGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAAc                              <  1:250254/93‑1 (MQ=255)

                                                           ccGAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAAc                              <  1:3884870/93‑1 (MQ=255)

                                                             gAATGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTg                            >  2:5813720/1‑93 (MQ=255)

                                                             gAATGCGCCTTAGAAGATGCCGCCCGCTACGCCAACCAGCGTATGGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTg                            <  1:3779998/93‑1 (MQ=255)

                                                              aaTGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTgg                           <  2:3139635/93‑1 (MQ=255)

                                                               aTGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGgc                          >  2:2851473/1‑93 (MQ=255)

                                                                tGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGgcg                         >  2:3996462/1‑93 (MQ=255)

                                                                tGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGgcg                         >  2:3377173/1‑93 (MQ=255)

                                                                tGCGCCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGgcg                         >  2:1761550/1‑93 (MQ=255)

                                                                 gcgcCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGgcgc                        <  1:238094/93‑1 (MQ=255)

                                                                  cgcCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCGATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCt                       <  1:670395/93‑1 (MQ=255)

                                                                  cgcCTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCt                       <  2:5274681/93‑1 (MQ=255)

                                                                    ccTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGa                     <  1:5268727/93‑1 (MQ=255)

                                                                     cTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGAt                    <  2:4350125/93‑1 (MQ=255)

                                                                     cTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGAt                    <  1:4722678/93‑1 (MQ=255)

                                                                     cTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGAt                    >  1:5516615/1‑93 (MQ=255)

                                                                     cTTTGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGAt                    >  1:5967146/1‑93 (MQ=255)

                                                                      tttGAAGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATg                   <  1:1931251/93‑1 (MQ=255)

                                                                          aaGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGa               <  1:5775213/93‑1 (MQ=255)

                                                                           aGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGAt              >  1:4301767/1‑93 (MQ=255)

                                                                           aGATGCCGCCCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGAt              >  2:1841123/1‑93 (MQ=255)

                                                                              agccgccCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTaa           <  2:3971115/92‑1 (MQ=255)

                                                                               gccgccCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAg          <  1:3923717/93‑1 (MQ=255)

                                                                               gccgccCGCTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAg          <  1:6253223/93‑1 (MQ=255)

                                                                                ccgcccgcTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGa         <  2:498644/93‑1 (MQ=255)

                                                                                 cgcccgcTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGAt        <  1:1820857/93‑1 (MQ=255)

                                                                                  gcccgcTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGGGCTGATGGCGATTAAGATc       >  1:166082/1‑93 (MQ=255)

                                                                                    ccgcTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGa     <  1:6732621/93‑1 (MQ=255)

                                                                                      gcTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGaca   >  2:1887309/1‑93 (MQ=255)

                                                                                       cTACGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGacaa  <  1:1510203/93‑1 (MQ=255)

TGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAA  >  NC_000913/1775918‑1776097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`