BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,533	C→T	A99V (GCG→GTG)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,533	0	C	T	99.6%	1042.5 / ‑5.5	278	A99V (GCG→GTG)	talB	transaldolase B
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (123/154); total (123/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.18e-01

Only 100 of 290 total aligned reads displayed.

AGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTA  >  NC_000913/8443‑8622

agATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATgtgc                                                                                         >  1:3620438/1‑93 (MQ=255)

 gATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGtgcg                                                                                        >  2:5476816/1‑93 (MQ=255)

   tCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTc                                                                                      >  2:4743390/1‑93 (MQ=255)

      tGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCttt                                                                                   <  1:1668262/93‑1 (MQ=255)

       ggACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTc                                                                                  <  1:3475197/93‑1 (MQ=255)

       ggACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTc                                                                                  >  2:6237637/1‑93 (MQ=255)

        gACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTcc                                                                                 <  2:1068428/93‑1 (MQ=255)

         aCGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTAAACTGAAGTTGATGTGCGTCTTTCCt                                                                                >  2:1266334/1‑93 (MQ=255)

          cgcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTa                                                                               <  1:686416/93‑1 (MQ=255)

          cgcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTa                                                                               <  1:5380516/93‑1 (MQ=255)

          cgcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTa                                                                               <  1:4134634/93‑1 (MQ=255)

             gaccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGa                                                                            <  1:4536202/93‑1 (MQ=255)

               ccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGaca                                                                          >  2:3754967/1‑93 (MQ=255)

                cgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGacac                                                                         >  2:878536/1‑93 (MQ=255)

                 gacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACAcc                                                                        >  2:1945337/1‑93 (MQ=255)

                 gacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACAcc                                                                        <  2:2982655/93‑1 (MQ=255)

                  acAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTACGTCTTTCCTATGACACCg                                                                       <  1:6545644/93‑1 (MQ=255)

                    aaaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGaa                                                                     >  2:2838609/1‑93 (MQ=255)

                    aaaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGaa                                                                     >  1:1333795/1‑93 (MQ=255)

                    aaaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGaa                                                                     >  1:3655127/1‑93 (MQ=255)

                     aaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAg                                                                    >  2:2879189/1‑93 (MQ=255)

                     aaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAg                                                                    <  2:5071438/93‑1 (MQ=255)

                      aCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGc                                                                   <  1:3501392/93‑1 (MQ=255)

                      aCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGc                                                                   <  1:5020659/93‑1 (MQ=255)

                      aCTGGCAGTAAATATTGGTCGGAAAACCCTGAAACTGGTTCGGGGCCGTATCTAAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGc                                                                   <  1:6378642/93‑1 (MQ=255)

                       cTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCg                                                                  >  1:464805/1‑93 (MQ=255)

                       cTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCg                                                                  >  1:4848603/1‑93 (MQ=255)

                            aGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAAt                                                             <  1:3057545/93‑1 (MQ=255)

                            aGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAAt                                                             <  1:6131900/93‑1 (MQ=255)

                               aaaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGc                                                          <  2:2946310/93‑1 (MQ=255)

                               aaaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGc                                                          <  2:4023368/93‑1 (MQ=255)

                                aaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCg                                                         <  2:3837287/93‑1 (MQ=255)

                                aaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCg                                                         >  1:244319/1‑93 (MQ=255)

                                 atatTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGa                                                        >  2:6492344/1‑93 (MQ=255)

                                  tatTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaa                                                       <  1:3958353/93‑1 (MQ=255)

                                   tttGGTCTGGAAATCCTGAAGCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaaa                                                      <  1:339307/92‑1 (MQ=255)

                                   atgggTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaaa                                                      <  2:1606713/90‑1 (MQ=255)

                                    ttGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAg                                                     <  2:3438950/93‑1 (MQ=255)

                                     tGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGc                                                    <  1:2977032/93‑1 (MQ=255)

                                       gTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaa                                                  <  2:3548118/93‑1 (MQ=255)

                                        tCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaa                                                 >  2:6567171/1‑93 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                               <  1:5701552/93‑1 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                               <  2:4309523/93‑1 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                               <  1:221291/93‑1 (MQ=255)

                                           ggAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACg                                              >  2:3281053/1‑93 (MQ=255)

                                              aaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCt                                           >  2:2234028/1‑93 (MQ=255)

                                               aTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTg                                          >  2:4833623/1‑93 (MQ=255)

                                                tCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                         <  1:5478257/93‑1 (MQ=255)

                                                tCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                         <  2:5930057/93‑1 (MQ=255)

                                                 ccTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGAt                                        <  2:306852/93‑1 (MQ=255)

                                                  cTGAACCTGGTTGCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATc                                       <  1:2712411/93‑1 (MQ=255)

                                                  cTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATc                                       <  1:1041972/93‑1 (MQ=255)

                                                  cTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATc                                       <  2:6449801/93‑1 (MQ=255)

                                                    gAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCaa                                     >  2:5152653/1‑93 (MQ=255)

                                                     aaaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCaaa                                    <  1:3910348/93‑1 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                   >  1:1161083/1‑93 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                   >  2:2727258/1‑93 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCATCAATTGCGAAAGCAAAACGCCTGATCAAAc                                   <  2:1413056/93‑1 (MQ=255)

                                                       aCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAct                                  >  2:5772773/1‑93 (MQ=255)

                                                        cTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActc                                 <  1:4597230/93‑1 (MQ=255)

                                                         tGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActct                                >  2:2449556/1‑93 (MQ=255)

                                                          ggTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTa                               >  1:4748767/1‑93 (MQ=255)

                                                           gTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTAc                              >  2:5581909/1‑93 (MQ=255)

                                                           gTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTAc                              <  2:4113194/93‑1 (MQ=255)

                                                           gTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTAc                              >  2:3894444/1‑93 (MQ=255)

                                                           gTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTAc                              <  2:575711/93‑1 (MQ=255)

                                                            ttCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACa                             >  1:5612916/1‑93 (MQ=255)

                                                              ccGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAAc                           <  2:1486260/93‑1 (MQ=255)

                                                                gggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGa                         >  1:2554727/1‑93 (MQ=255)

                                                                gggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGa                         <  2:6341591/93‑1 (MQ=255)

                                                                 ggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGAt                        >  1:5798428/1‑93 (MQ=255)

                                                                 ggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGAt                        <  1:2422356/93‑1 (MQ=255)

                                                                 ggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGAt                        >  1:2034472/1‑93 (MQ=255)

                                                                 ggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGAt                        >  2:4358136/1‑93 (MQ=255)

                                                                     gTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTg                    >  1:1216966/1‑93 (MQ=255)

                                                                     gTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTg                    >  1:3147807/1‑93 (MQ=255)

                                                                     gTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTg                    >  1:4382077/1‑93 (MQ=255)

                                                                       aTCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGt                  >  1:1434334/1‑93 (MQ=255)

                                                                        tctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTa                 <  2:6640779/93‑1 (MQ=255)

                                                                         ctcAACTGAAGTTGATGTGCGTCTTTCCTATGACCCCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAt                <  2:4997118/93‑1 (MQ=255)

                                                                          tcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAtt               <  2:2583109/93‑1 (MQ=255)

                                                                          tcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAtt               <  1:92300/93‑1 (MQ=255)

                                                                           cAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTa              <  1:1465316/93‑1 (MQ=255)

                                                                            aaCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAg             >  1:527247/1‑93 (MQ=255)

                                                                            aaCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAg             >  2:5216971/1‑93 (MQ=255)

                                                                             aCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGc            <  2:6355244/93‑1 (MQ=255)

                                                                             aCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGc            <  1:843246/93‑1 (MQ=255)

                                                                             aCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGc            <  1:5932723/93‑1 (MQ=255)

                                                                             aCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGc            >  1:2910592/1‑93 (MQ=255)

                                                                               tGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCaa          <  2:5370944/93‑1 (MQ=255)

                                                                                gAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAAc         <  2:801016/93‑1 (MQ=255)

                                                                                 aaGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACg        <  1:1551686/93‑1 (MQ=255)

                                                                                  aGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGa       <  2:5812457/93‑1 (MQ=255)

                                                                                   gTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGAt      <  2:1722182/93‑1 (MQ=255)

                                                                                   gTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGAt      >  2:4502675/1‑93 (MQ=255)

                                                                                   gTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGAt      >  2:3156867/1‑93 (MQ=255)

                                                                                      gATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGt   >  1:25179/1‑93 (MQ=255)

                                                                                       aTGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCACCGATCGTa  >  2:3191996/1‑93 (MQ=255)

                                                                                       aTGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTa  <  2:6141007/93‑1 (MQ=255)

                                                                                       aTGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTa  <  1:422316/93‑1 (MQ=255)

AGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTA  >  NC_000913/8443‑8622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`