Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,909,255 T→C V319A (GTG→GCG)  b1828 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,909,2550TC99.5% 667.3 / ‑5.1 208V319A (GTG→GCG) b1828putative transport protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (112/95);  minor base A (0/1);  total (112/96)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 218 total aligned reads displayed.

TTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTT  >  NC_000913/1909165‑1909344
                                                                                          |                                                                                         
tttCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGa                                                                                         >  2:561935/1‑93 (MQ=255)
     cTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAAcgatggcgatggat                                                                                    >  2:6278558/1‑91 (MQ=255)
     cTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCt                                                                                    >  1:5872312/1‑93 (MQ=255)
     cTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCt                                                                                    >  2:4101364/1‑93 (MQ=255)
         ccTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGc                                                                                >  2:4298941/1‑93 (MQ=255)
         ccTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGc                                                                                >  1:2490741/1‑93 (MQ=255)
          cTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCt                                                                               >  1:1057994/1‑93 (MQ=255)
          cTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCt                                                                               >  2:336554/1‑93 (MQ=255)
          cTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCt                                                                               >  2:3728412/1‑93 (MQ=255)
          cTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCt                                                                               >  2:4605587/1‑93 (MQ=255)
          cTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCt                                                                               >  2:6231105/1‑93 (MQ=255)
           ttttACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTg                                                                              >  2:6423842/1‑93 (MQ=255)
             ttACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGc                                                                            >  1:141306/1‑93 (MQ=255)
              tACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCa                                                                           <  1:4297351/93‑1 (MQ=255)
               aCCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAg                                                                          >  1:6197938/1‑93 (MQ=255)
                ccTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAgg                                                                         <  2:3455450/93‑1 (MQ=255)
                 cTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGc                                                                        <  1:1561881/93‑1 (MQ=255)
                  tGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCt                                                                       <  1:6113116/93‑1 (MQ=255)
                   gCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTa                                                                      <  2:1864912/93‑1 (MQ=255)
                     aaaCCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATTGCGATGGCTCCGCTGGCAGGCTAtt                                                                    >  1:5180196/1‑93 (MQ=255)
                     aaaCCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTAtt                                                                    <  1:6434084/93‑1 (MQ=255)
                     aaaCCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTAtt                                                                    >  2:1426045/1‑93 (MQ=255)
                      aaCCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTAttt                                                                   >  2:6472062/1‑93 (MQ=255)
                      aaCCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTAttt                                                                   >  1:5645059/1‑93 (MQ=255)
                       aCCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATttg                                                                  >  1:4725898/1‑93 (MQ=255)
                       aCCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATttg                                                                  >  1:5760885/1‑93 (MQ=255)
                         cGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCGGGCTATttgat                                                                <  1:2207953/93‑1 (MQ=255)
                         cGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATttgat                                                                >  1:1662618/1‑93 (MQ=255)
                          gTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATttgatt                                                               >  2:3029049/1‑93 (MQ=255)
                          gTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATttgatt                                                               <  2:267926/93‑1 (MQ=255)
                          gTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATttgatt                                                               >  1:2782805/1‑93 (MQ=255)
                            gCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATttgattga                                                             <  2:1356210/93‑1 (MQ=255)
                            gCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATttgattga                                                             <  1:5132684/93‑1 (MQ=255)
                             cTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGaa                                                            <  2:1971370/93‑1 (MQ=255)
                             cTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGaa                                                            <  2:6639868/93‑1 (MQ=255)
                             cTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGaa                                                            <  2:4454970/93‑1 (MQ=255)
                             cTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGaa                                                            <  2:5046282/93‑1 (MQ=255)
                              tCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAAc                                                           <  2:3825943/93‑1 (MQ=255)
                               cGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAAcg                                                          <  2:5752403/93‑1 (MQ=255)
                                   cGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTa                                                      <  1:3117490/93‑1 (MQ=255)
                                    gTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTAc                                                     >  1:1953935/1‑93 (MQ=255)
                                     tAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACa                                                    >  1:779470/1‑93 (MQ=255)
                                     tAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACa                                                    >  1:2444311/1‑93 (MQ=255)
                                       gTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATg                                                  >  2:70866/1‑93 (MQ=255)
                                          aaGTCGAAACAGGTTTAGTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGGAg                                               >  1:3643208/1‑93 (MQ=255)
                                          aaGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATTCAg                                               >  1:6527494/1‑93 (MQ=255)
                                          aaGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAg                                               <  1:1779413/93‑1 (MQ=255)
                                          aaGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAg                                               <  2:1107384/93‑1 (MQ=255)
                                          aaGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAg                                               >  2:2804760/1‑93 (MQ=255)
                                          aaGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAg                                               <  2:2199211/93‑1 (MQ=255)
                                            gTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGa                                             >  1:3238336/1‑93 (MQ=255)
                                            gTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGa                                             >  2:2832166/1‑93 (MQ=255)
                                            atCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGa                                             <  2:3340157/92‑1 (MQ=255)
                                              cGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGGTTGAACGCGTACATGCAGGAtt                                           >  2:3136515/1‑93 (MQ=255)
                                              cGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGAtt                                           <  1:4612053/93‑1 (MQ=255)
                                               gAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTg                                          >  1:2010384/1‑93 (MQ=255)
                                                 aaCAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCt                                        <  1:1898440/93‑1 (MQ=255)
                                                  aCAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTg                                       >  1:3400484/1‑93 (MQ=255)
                                                    aGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTggg                                     >  1:4984815/1‑93 (MQ=255)
                                                     ggTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTgggg                                    <  2:4804401/93‑1 (MQ=255)
                                                        ttACTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCt                                 <  2:6147174/93‑1 (MQ=255)
                                                         tACTTCTGACACCGTGGCCGTTAGCAATGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCtt                                <  1:3012372/93‑1 (MQ=255)
                                                          aCTTCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCttt                               >  1:2601275/1‑93 (MQ=255)
                                                             tCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAgg                            <  1:5978124/93‑1 (MQ=255)
                                                             tCTGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAgg                            >  2:3259040/1‑93 (MQ=255)
                                                               tGACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGgt                          <  1:4651236/93‑1 (MQ=255)
                                                                gACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGgtt                         >  1:3794165/1‑93 (MQ=255)
                                                                gACACCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGgtt                         <  1:4376034/93‑1 (MQ=255)
                                                                 acacCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGgttg                        <  1:4246361/93‑1 (MQ=255)
                                                                  cacCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGgttgt                       <  1:4990024/93‑1 (MQ=255)
                                                                  cacCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGgttgt                       >  1:3973732/1‑93 (MQ=255)
                                                                   acCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGgttgtt                      >  1:5416199/1‑93 (MQ=255)
                                                                   acCGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGgttgtt                      >  2:3547224/1‑93 (MQ=255)
                                                                    ccGTGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTtc                     >  2:516263/1‑93 (MQ=255)
                                                                       tGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTtcatc                  >  2:656797/1‑93 (MQ=255)
                                                                       tGGCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTtcatc                  >  2:1725690/1‑93 (MQ=255)
                                                                        ggCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTCGGGTTGTtcatca                 >  1:6663282/1‑93 (MQ=255)
                                                                        ggCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTtcatca                 >  1:6400414/1‑93 (MQ=255)
                                                                         gCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTcatcat                >  2:6365324/1‑93 (MQ=255)
                                                                         gCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTcatcat                <  2:6095007/93‑1 (MQ=255)
                                                                         gCCGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTcatcat                >  1:3376138/1‑93 (MQ=255)
                                                                          ccGTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATg               <  1:3166270/93‑1 (MQ=255)
                                                                            gTTAGCAACGATGGCGATGTCTCCGCTGGCAGGCTATTTGATTGACCGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGc             <  1:1237397/93‑1 (MQ=255)
                                                                            gTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGc             <  1:1808930/93‑1 (MQ=255)
                                                                            gTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGc             >  2:1188656/1‑93 (MQ=255)
                                                                            gTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGc             <  2:5720515/93‑1 (MQ=255)
                                                                            gTTAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGc             >  2:3994883/1‑93 (MQ=255)
                                                                             ttAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCt            >  2:233255/1‑93 (MQ=255)
                                                                             ttAGCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGGTGTTCATCATGGCt            >  2:6196919/1‑93 (MQ=255)
                                                                                gCAACGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCg         <  1:321881/93‑1 (MQ=255)
                                                                                  aaCGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCggg       <  2:3762954/93‑1 (MQ=255)
                                                                                  aaCGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCggg       >  1:2924237/1‑93 (MQ=255)
                                                                                   aCGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCgggg      >  2:1072643/1‑93 (MQ=255)
                                                                                   aCGATGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCgggg      >  1:6627760/1‑93 (MQ=255)
                                                                                    cgatggcgatggCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGc     >  1:143698/1‑93 (MQ=255)
                                                                                      aTGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCtt   <  2:1839599/93‑1 (MQ=255)
                                                                                      aTGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCtt   <  2:2769268/93‑1 (MQ=255)
                                                                                      aTGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCtt   <  2:5290807/93‑1 (MQ=255)
                                                                                       tGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCttt  <  1:1847/93‑1 (MQ=255)
                                                                                       tGGCGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCttt  <  2:433017/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTTCCCTGCCCTTTTACCTGCAAACCGTGCTCGGGCGTAGTGAAGTCGAAACAGGTTTACTTCTGACACCGTGGCCGTTAGCAACGATGGTGATGGCTCCGCTGGCAGGCTATTTGATTGAACGCGTACATGCAGGATTGCTGGGGGCTTTAGGGTTGTTCATCATGGCTGCGGGGCTTT  >  NC_000913/1909165‑1909344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑