BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	204,590	T→A	I33N (ATC→AAC)	rnhB →	RNAse HII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	204,590	0	T	A	99.5%	735.4 / NA	214	I33N (ATC→AAC)	rnhB	RNAse HII
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (78/135); minor base C (0/1); total (78/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

AATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAG  >  NC_000913/204500‑204679

aaTTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAAcc                                                                                              <  1:3192084/93‑1 (MQ=255)

 aTTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCt                                                                                             <  1:4231725/93‑1 (MQ=255)

 aTTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCt                                                                                             <  2:2205977/93‑1 (MQ=255)

 aTTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCt                                                                                             <  2:2957006/93‑1 (MQ=255)

  tttGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCtt                                                                                            <  2:4058916/93‑1 (MQ=255)

   ttGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTg                                                                                           <  2:5321826/93‑1 (MQ=255)

     gTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGCTAGTTGGCGCGGTCGTCACCGCTGCGGTGAATCTTGAc                                                                                         >  2:2329538/1‑93 (MQ=255)

     gTTTATCCGCACACGCAGCTGGGTGC‑GGTGTGGATGAAGTCGGACGCGGGGCGTTAGGTGGCGCGGTCGACACCGCTGCGGGGAACCTTGAcg                                                                                        >  1:1124110/1‑92 (MQ=255)

      tttATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGAcc                                                                                        <  1:5876740/93‑1 (MQ=255)

      tttATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGAcc                                                                                        <  2:6430617/93‑1 (MQ=255)

      tttATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGAcc                                                                                        >  1:6443639/1‑93 (MQ=255)

       ttATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGAccc                                                                                       <  1:6016481/93‑1 (MQ=255)

       ttATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGAccc                                                                                       <  1:3084194/93‑1 (MQ=255)

          tCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGgc                                                                                    <  2:5958962/93‑1 (MQ=255)

          tCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGgc                                                                                    <  1:4892937/93‑1 (MQ=255)

           ccGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGgcg                                                                                   >  2:6693863/1‑93 (MQ=255)

            cGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGgcgc                                                                                  >  2:6117065/1‑93 (MQ=255)

            cGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGgcgc                                                                                  >  1:6298625/1‑93 (MQ=255)

             gCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGgcgcg                                                                                 >  1:6148547/1‑93 (MQ=255)

              cacaCGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGgcgcgc                                                                                >  1:361705/1‑93 (MQ=255)

               acacGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGCACCTTGACCCGgcgcgtc                                                                               >  1:4645896/1‑91 (MQ=255)

               acacGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGcc                                                                               <  1:5634104/93‑1 (MQ=255)

                cacGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGccc                                                                              <  1:6306028/93‑1 (MQ=255)

                cacGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGccc                                                                              <  2:3139851/93‑1 (MQ=255)

                   gCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑t                                                                          <  2:3399889/93‑1 (MQ=255)

                    cAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑tt                                                                         <  2:2022150/93‑1 (MQ=255)

                    cAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑tt                                                                         <  2:6175893/93‑1 (MQ=255)

                     aGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGC‑TTg                                                                        >  1:3865331/1‑93 (MQ=255)

                     aGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑GTg                                                                        >  2:4772550/1‑93 (MQ=255)

                      gCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑tttc                                                                       >  2:5592786/1‑91 (MQ=255)

                       cTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑cc                                                                     <  1:3714103/93‑1 (MQ=255)

                        tGGTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCg                                                                    <  1:6690469/93‑1 (MQ=255)

                         ggTTGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCgg                                                                   >  2:872188/1‑93 (MQ=255)

                           ttGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGc                                                                 >  2:821382/1‑93 (MQ=255)

                           ttGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGc                                                                 <  2:5269470/93‑1 (MQ=255)

                           ttGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGc                                                                 <  2:517576/93‑1 (MQ=255)

                            tGCGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCt                                                                <  1:5083849/93‑1 (MQ=255)

                             gCGGGTGTGGATGAAGTCGGCCGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGccg                                                               >  1:2785850/1‑91 (MQ=255)

                              cGGGTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑Ga                                                             >  1:2045241/1‑93 (MQ=255)

                               gggTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCGCCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑Gaa                                                            <  1:5939574/93‑1 (MQ=255)

                                ggTGTGGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGCGCT‑GCAt                                                           >  2:6353461/1‑93 (MQ=255)

                                   gtgGATGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑At                                                       >  1:5247287/1‑93 (MQ=255)

                                   gtgGAGGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGTT‑GAATG‑At                                                       <  1:6549800/93‑1 (MQ=255)

                                       aTGAAGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCa                                                  <  1:3991872/93‑1 (MQ=255)

                                           aGTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCaaaaa                                              <  1:1347680/93‑1 (MQ=255)

                                            gTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGG‑TTG‑CCGGGGT‑GAATG‑ATT‑CCaaaaaa                                             >  2:1337878/1‑92 (MQ=255)

                                            gTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATT‑ATT‑CCaaaaaa                                             >  1:6031465/1‑92 (MQ=255)

                                            gTCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCACTGCGGTGAACCTTGACCCGGCGCGGCCGG‑GGGACCGGGC‑‑GAAGG‑ATT‑CCAAAAAg                                             >  2:3679980/1‑93 (MQ=255)

                                             tCGGACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GGATG‑ATT‑TCaaaaaaa                                            >  2:1437385/1‑91 (MQ=255)

                                              cgcaCGCGGGGCGTTACTTGGCGCGGTCGTCACCGCTGCGGTGAACTTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCt                                           <  1:1662377/90‑1 (MQ=255)

                                               ggACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGGCCCA‑CGT‑GCGGGCT‑GGATGAATT‑CCaaaaaata                                           >  2:39092/1‑90 (MQ=255)

                                               ggACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTg                                          <  1:6248546/93‑1 (MQ=255)

                                                gACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGAGTTG‑CCGGG‑T‑GAAAG‑ATTACCaaaaacata                                          >  1:3787817/1‑89 (MQ=255)

                                                gACGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGa                                         <  1:337060/93‑1 (MQ=255)

                                                 aCGCGGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAg                                        <  1:4551341/93‑1 (MQ=255)

                                                  cgcgGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGc                                       <  2:852062/93‑1 (MQ=255)

                                                  cgcgGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGc                                       <  2:1754708/93‑1 (MQ=255)

                                                   gcgGGGCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCCC‑ATG‑CCCGGCTCGAAGG‑CTC‑CCAAAAAACtaacg                                       >  2:3531240/1‑89 (MQ=255)

                                                      gggcGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGC‑TTT‑CCCGGCT‑GAATG‑AGT‑TCAAAAAACTGAGCGgaa                                   >  2:5844925/4‑93 (MQ=255)

                                                      gggcGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGaaa                                   >  1:1077131/4‑93 (MQ=255)

                                                      gggcGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAACTGAGCGgaa                                   >  1:1589630/4‑93 (MQ=255)

                                                        gcGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGaaaaa                                 <  1:4174464/92‑1 (MQ=255)

                                                         cGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAACTGAGCGaaaaaa                                >  2:5051563/1‑92 (MQ=255)

                                                         cGTTAGTCGGCGCGTTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAAc                                <  1:4034493/93‑1 (MQ=255)

                                                          gTTAGTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACg                               >  1:749873/1‑93 (MQ=255)

                                                              gTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGt                           <  2:6132763/93‑1 (MQ=255)

                                                              gTTGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAAAGCCGt                           >  1:4377906/1‑93 (MQ=255)

                                                               ttGGCGCGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTc                          <  1:4106395/93‑1 (MQ=255)

                                                                tGGCGCGTTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCt                         <  1:1705821/93‑1 (MQ=255)

                                                                  gcgcGGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTgg                       <  1:6624016/93‑1 (MQ=255)

                                                                   cgcgGTCTTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGgc                      <  1:2077193/93‑1 (MQ=255)

                                                                   cgcgGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGgc                      <  2:167596/93‑1 (MQ=255)

                                                                    gcgGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTgggg                     >  1:872257/1‑91 (MQ=255)

                                                                     cgGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGgcgc                    <  1:6474943/93‑1 (MQ=255)

                                                                     cgGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGgcgc                    <  1:5662236/93‑1 (MQ=255)

                                                                     cgGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGgcgc                    <  1:6349842/93‑1 (MQ=255)

                                                                     cgGTCGTCACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGgcgc                    <  2:5705983/93‑1 (MQ=255)

                                                                       gtcgtcACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGctc                  >  2:4338216/1‑93 (MQ=255)

                                                                       gtcgtcACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGctc                  >  2:2884663/1‑93 (MQ=255)

                                                                       gtcgtcACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGctc                  >  2:1143101/1‑93 (MQ=255)

                                                                       gtcgtcACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGctc                  <  2:1989572/93‑1 (MQ=255)

                                                                        tcgtcACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGctct                 <  2:448265/93‑1 (MQ=255)

                                                                         cgtcACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTa                <  1:4978770/93‑1 (MQ=255)

                                                                          gtcACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTAt               <  1:4769667/93‑1 (MQ=255)

                                                                           tcACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATg              <  1:4370295/93‑1 (MQ=255)

                                                                           tcACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATg              >  2:4090503/1‑93 (MQ=255)

                                                                            cACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGa             <  1:4510239/93‑1 (MQ=255)

                                                                            cACCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGa             <  1:3243358/93‑1 (MQ=255)

                                                                             cccGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGaa            <  1:182868/92‑1 (MQ=255)

                                                                             aCCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑TCAAAAAACTGAGCGAAAAACGCCGTCTAGCGCTCTATGaa            >  2:6473468/1‑93 (MQ=255)

                                                                             aCCGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGaa            <  2:1083578/93‑1 (MQ=255)

                                                                              ccGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAag           <  2:17283/93‑1 (MQ=255)

                                                                              ccGCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAag           <  1:2418720/93‑1 (MQ=255)

                                                                                gCTGCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAagag         <  1:2285764/93‑1 (MQ=255)

                                                                                   gCGGTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATc      >  2:3178956/1‑93 (MQ=255)

                                                                                    cGGTGAACCGTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCa     <  1:1254509/93‑1 (MQ=255)

                                                                                     ggTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCCAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATcca    >  2:4194425/1‑91 (MQ=255)

                                                                                      gTGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCaaa   <  1:2941706/93‑1 (MQ=255)

                                                                                       tGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAag  <  2:4636752/93‑1 (MQ=255)

                                                                                       tGAACCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAag  <  1:281169/93‑1 (MQ=255)

AATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGCGGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGA‑TTG‑CCGGGCT‑GAATG‑ATT‑CCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAGATCAAAG  >  NC_000913/204500‑204679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`