BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,713	T→C	S92S (AGT→AGC)	yebI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,713	0	T	C	99.6%	709.5 / ‑4.7	223	S92S (AGT→AGC)	yebI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (92/130); total (92/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 228 total aligned reads displayed.

GTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAGT  >  NC_000913/1941624‑1941802

 ttACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGccc                                                                                       <  1:6474217/93‑1 (MQ=255)

 ttACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGccc                                                                                       <  1:3492393/93‑1 (MQ=255)

  tACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCt                                                                                      >  2:3156433/1‑93 (MQ=255)

   aCGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTg                                                                                     >  2:6632149/1‑93 (MQ=255)

    cGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGCAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCTCCCTGt                                                                                    <  2:2070626/93‑1 (MQ=255)

     gctgctgctGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTc                                                                                   <  2:6327155/93‑1 (MQ=255)

      ctgctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCg                                                                                  <  1:6013202/93‑1 (MQ=255)

       tgctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGc                                                                                 <  1:5963622/93‑1 (MQ=255)

        gctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCt                                                                                >  1:1801268/1‑93 (MQ=255)

        gctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCt                                                                                <  2:4682344/93‑1 (MQ=255)

        gctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCt                                                                                >  2:2257766/1‑93 (MQ=255)

         ctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTg                                                                               <  2:4856039/93‑1 (MQ=255)

          tgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTgg                                                                              >  1:1702294/1‑93 (MQ=255)

          tgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTgg                                                                              <  1:4759255/93‑1 (MQ=255)

          tgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTgg                                                                              >  1:6120634/1‑93 (MQ=255)

            ctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGc                                                                            <  1:3080899/93‑1 (MQ=255)

             tgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGcc                                                                           <  1:2790774/93‑1 (MQ=255)

             tcgcgggcggTCTGGTATGGCTGGACAACCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGcc                                                                           <  1:73044/91‑1 (MQ=255)

              ggcgggcgGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGTCt                                                                          >  2:219804/1‑93 (MQ=255)

              ggcgggcgGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCt                                                                          >  2:2630622/1‑93 (MQ=255)

              ggcgggcgGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCt                                                                          >  2:3880531/1‑93 (MQ=255)

                cgggcggTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCtgg                                                                        <  2:5944954/93‑1 (MQ=255)

                   gcggTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCtggtgg                                                                     <  1:5576086/93‑1 (MQ=255)

                    cggTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTggtggt                                                                    <  2:6430975/93‑1 (MQ=255)

                     ggTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTc                                                                   >  2:3485685/1‑93 (MQ=255)

                         tGGTATGGCTGGAGAAGCGTCGACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGa                                                               <  1:2675610/93‑1 (MQ=255)

                         tGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCTCTGGTCCTGGAGGTCGTGa                                                               <  1:6563744/93‑1 (MQ=255)

                         tGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGa                                                               >  2:1414087/1‑93 (MQ=255)

                         tGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGa                                                               <  1:4488054/93‑1 (MQ=255)

                          ggTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAg                                                              <  1:3334994/93‑1 (MQ=255)

                          ggTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAg                                                              <  1:5185101/93‑1 (MQ=255)

                          ggTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATGAGGGATTATGGCGCCCAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAg                                                              <  2:3216428/93‑1 (MQ=255)

                            tATGGATGGAGAAACGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTc                                                            <  2:1814082/93‑1 (MQ=255)

                            gaTGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTc                                                            <  2:4434115/92‑1 (MQ=255)

                             aTGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGGCTGGTGGTCGTGAGTCt                                                           >  2:2356155/1‑93 (MQ=255)

                             aTGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCt                                                           <  1:1294460/93‑1 (MQ=255)

                             aTGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCt                                                           >  1:2112429/1‑93 (MQ=255)

                                gCTGGAGAAGCGTCCACAGCTGGCGGTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGAt                                                        <  1:3481639/93‑1 (MQ=255)

                                gCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGAt                                                        <  1:474198/93‑1 (MQ=255)

                                gCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGAt                                                        <  1:1768121/93‑1 (MQ=255)

                                 cTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATg                                                       <  2:5771519/93‑1 (MQ=255)

                                   ggAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTc                                                     >  1:5490566/1‑93 (MQ=255)

                                   ggAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTc                                                     <  1:1667343/93‑1 (MQ=255)

                                   ggAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTc                                                     <  2:3086444/93‑1 (MQ=255)

                                     agaAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCATGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTa                                                   >  1:2099471/1‑93 (MQ=255)

                                      gaAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTaa                                                  <  2:4956639/93‑1 (MQ=255)

                                        aGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAata                                                >  1:1458937/1‑93 (MQ=255)

                                        aGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAata                                                <  2:2891502/93‑1 (MQ=255)

                                        aGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAata                                                <  2:3774646/93‑1 (MQ=255)

                                         gCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAatat                                               >  2:35708/1‑93 (MQ=255)

                                             ccACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgt                                           >  1:5857687/1‑93 (MQ=255)

                                               acaGCTGGCGATCGACACGTAATTAGGGATTATGGCGCACAGCGCCATGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgtgt                                         <  1:758576/93‑1 (MQ=255)

                                                caGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGtt                                        <  1:5348026/93‑1 (MQ=255)

                                                caGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGtt                                        >  1:6246812/1‑93 (MQ=255)

                                                caGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGtt                                        <  1:6515056/93‑1 (MQ=255)

                                                caGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGtt                                        >  1:3941900/1‑93 (MQ=255)

                                                 aGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttg                                       <  1:6401108/93‑1 (MQ=255)

                                                  gCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttga                                      >  1:6713435/1‑93 (MQ=255)

                                                  gCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttga                                      <  1:3544092/93‑1 (MQ=255)

                                                    tGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCCGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatt                                    >  1:2921307/1‑93 (MQ=255)

                                                     ggCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgattt                                   <  1:3996836/93‑1 (MQ=255)

                                                     ggCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgattt                                   <  2:2230357/93‑1 (MQ=255)

                                                      gCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttg                                  <  2:2808196/93‑1 (MQ=255)

                                                       cGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttga                                 <  2:4740799/93‑1 (MQ=255)

                                                       cGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttga                                 >  2:447337/1‑93 (MQ=255)

                                                       cGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttga                                 <  1:4055913/93‑1 (MQ=255)

                                                        gATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttgat                                <  2:5222877/93‑1 (MQ=255)

                                                        gATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttgat                                >  2:4006893/1‑93 (MQ=255)

                                                         aTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATg                               <  2:6199927/93‑1 (MQ=255)

                                                          tCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATgg                              >  2:1288168/1‑93 (MQ=255)

                                                           cGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGACTAATATTCGTGTTGATTTGCTGGc                             >  1:1084451/1‑93 (MQ=255)

                                                            gACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCt                            >  2:1020150/1‑93 (MQ=255)

                                                            gACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCt                            >  2:452655/1‑93 (MQ=255)

                                                            gACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCt                            >  1:4188455/1‑93 (MQ=255)

                                                             acacGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCtt                           <  2:836085/93‑1 (MQ=255)

                                                               acGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTAc                         >  1:5907344/1‑93 (MQ=255)

                                                               acGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTAc                         >  2:1707255/1‑93 (MQ=255)

                                                                cGTTATTAGGGATTGTGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTAcc                        <  1:5006120/93‑1 (MQ=255)

                                                                 gTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTTATATTCGTGTTGATTTGATGGCTTACCt                       >  2:1166551/1‑93 (MQ=255)

                                                                  ttattaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTg                      >  2:3954464/1‑93 (MQ=255)

                                                                  ttattaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTg                      >  2:4338372/1‑93 (MQ=255)

                                                                   tattaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTCCCTGt                     >  2:5191521/1‑93 (MQ=255)

                                                                    attaGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGtt                    <  2:5494619/93‑1 (MQ=255)

                                                                    attaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGtt                    >  1:5092573/1‑93 (MQ=255)

                                                                        gggATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGt                >  1:2148564/1‑93 (MQ=255)

                                                                        gggATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGt                >  1:3183898/1‑93 (MQ=255)

                                                                         ggATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTg               >  1:4814627/1‑93 (MQ=255)

                                                                          gATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGa              <  1:4560096/93‑1 (MQ=255)

                                                                           aTTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAt             >  1:81307/1‑93 (MQ=255)

                                                                            ttATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAtt            <  1:1951156/93‑1 (MQ=255)

                                                                              aTGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTg          >  1:4915658/1‑93 (MQ=255)

                                                                              aTGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTg          <  1:5727910/93‑1 (MQ=255)

                                                                               tGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGc         >  1:4368800/1‑93 (MQ=255)

                                                                                ggCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCt        >  1:2565889/1‑93 (MQ=255)

                                                                                 gcgcACAGCTCCCTGTCGCTGGGCCTGGTGTTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTg       <  1:2448664/93‑1 (MQ=255)

                                                                                 gcgcACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTg       <  2:3446310/93‑1 (MQ=255)

                                                                                 gcgcACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTg       <  2:731257/93‑1 (MQ=255)

                                                                                    cacaGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCa    <  1:531453/93‑1 (MQ=255)

                                                                                     acaGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAg   <  2:4612698/93‑1 (MQ=255)

                                                                                     acaGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAg   >  2:5584117/1‑93 (MQ=255)

GTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAGT  >  NC_000913/1941624‑1941802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`