BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,962,105	C→T	A324T (GCC→ACC)	flhA ←	FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,962,105	0	C	T	99.4%	639.1 / NA	180	A324T (GCC→ACC)	flhA	FlhA
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (69/110); minor base A (1/0); total (70/110)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

ACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTA  >  NC_000913/1962015‑1962194

aCGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCa                                                                                         >  1:5510404/1‑93 (MQ=255)

  gTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGc                                                                                       >  2:2729185/1‑93 (MQ=255)

   tCGCTTCGACAACGGTATTTTTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGcc                                                                                      <  2:3956945/93‑1 (MQ=255)

   tCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGcc                                                                                      <  2:5680427/93‑1 (MQ=255)

     gCTTCGAGAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCg                                                                                    <  2:6230140/93‑1 (MQ=255)

     gCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCg                                                                                    >  2:3161447/1‑93 (MQ=255)

      cTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGa                                                                                   <  1:3256550/93‑1 (MQ=255)

      cTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGa                                                                                   <  1:423890/93‑1 (MQ=255)

        tCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGc                                                                                 <  1:2448970/93‑1 (MQ=255)

        tCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGc                                                                                 >  2:5736206/1‑93 (MQ=255)

         cGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCa                                                                                <  1:3481448/93‑1 (MQ=255)

         cGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCa                                                                                <  1:5171155/93‑1 (MQ=255)

          gACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCaa                                                                               <  1:6245860/93‑1 (MQ=255)

          gACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCaa                                                                               >  1:1193557/1‑93 (MQ=255)

            cAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATc                                                                             <  2:3729070/93‑1 (MQ=255)

            cAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATc                                                                             <  2:6070251/93‑1 (MQ=255)

            cAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATc                                                                             >  1:1676812/1‑93 (MQ=255)

            cAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATc                                                                             <  2:4360846/93‑1 (MQ=255)

             aaCGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATcc                                                                            >  2:590137/1‑93 (MQ=255)

             aaCGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATcc                                                                            <  1:5308322/93‑1 (MQ=255)

                ggTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCggg                                                                         >  1:2910095/1‑92 (MQ=255)

                ggTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGc                                                                         <  1:3116940/93‑1 (MQ=255)

                ggTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGc                                                                         <  1:2516858/93‑1 (MQ=255)

                 gTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCCGGTCAGCCCGAGCCATGCGGCa                                                                        >  2:3068087/1‑93 (MQ=255)

                 gTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCa                                                                        <  2:6612712/93‑1 (MQ=255)

                   attattCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGt                                                                      >  2:4804563/1‑93 (MQ=255)

                    ttattCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTg                                                                     >  2:2871679/1‑93 (MQ=255)

                     tattCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGa                                                                    <  2:6666592/93‑1 (MQ=255)

                      attCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCCGCCCGAGCAATCCGGCAGTGaa                                                                   >  1:5412818/1‑93 (MQ=255)

                       ttCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAAc                                                                  <  1:6060439/93‑1 (MQ=255)

                       ttCTCTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAAc                                                                  <  1:3440974/93‑1 (MQ=255)

                        tctcTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACa                                                                 <  2:1917651/93‑1 (MQ=255)

                        tctcTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACa                                                                 <  2:123176/93‑1 (MQ=255)

                        tctcTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACa                                                                 <  2:4936651/93‑1 (MQ=255)

                        tctcTGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACa                                                                 >  2:6387371/1‑93 (MQ=255)

                          tctGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGc                                                               <  2:980181/93‑1 (MQ=255)

                          tctGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGAGAACAGc                                                               >  2:2484692/1‑93 (MQ=255)

                           ctGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCa                                                              >  1:3840341/1‑93 (MQ=255)

                            tGCCATTTTTACCGGTTTGGGTTCGGCTGGGGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCCGTGGACAGCaa                                                             >  1:729360/1‑93 (MQ=255)

                            tGCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGcca                                                             >  2:6293289/1‑91 (MQ=255)

                            tGCCATTTTTACCGGTGTGGGTGCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCaa                                                             <  2:228992/93‑1 (MQ=255)

                             gCCATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCaaa                                                            >  1:993695/1‑93 (MQ=255)

                               cATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCaaaaa                                                          >  2:4181733/1‑93 (MQ=255)

                               cATTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCaaaaa                                                          >  1:4932578/1‑93 (MQ=255)

                                aTTTTTACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAAt                                                         >  2:3098680/1‑93 (MQ=255)

                                  ttttACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATAc                                                       <  1:3318868/93‑1 (MQ=255)

                                  ttttACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATAc                                                       <  2:188417/93‑1 (MQ=255)

                                  tgttACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATAc                                                       <  1:2633004/91‑1 (MQ=255)

                                   tttACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATAcc                                                      <  1:1480520/93‑1 (MQ=255)

                                   tttACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATAcc                                                      <  1:6426994/93‑1 (MQ=255)

                                    ttACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCa                                                     <  1:5802442/93‑1 (MQ=255)

                                    ttACCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCa                                                     <  2:525872/93‑1 (MQ=255)

                                      aCCGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCAAGCCCGAGCCATCCGGCAGTGAACCGCAAAAATAACAgg                                                   >  2:1293871/1‑93 (MQ=255)

                                       ccGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGt                                                  <  2:2607739/93‑1 (MQ=255)

                                        cGGTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGtt                                                 <  1:4292821/93‑1 (MQ=255)

                                        cGGTGTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGtt                                                 <  2:5475860/93‑1 (MQ=255)

                                          gTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCg                                               <  1:3692830/93‑1 (MQ=255)

                                          gTTTGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCg                                               >  1:3574067/1‑93 (MQ=255)

                                             tGGGTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCa                                            <  2:6701556/93‑1 (MQ=255)

                                              gggTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCAt                                           <  1:4847633/93‑1 (MQ=255)

                                              gggTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCAt                                           >  2:5928717/1‑93 (MQ=255)

                                              gggTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCAt                                           <  2:1474744/93‑1 (MQ=255)

                                              gggTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCAt                                           <  1:3939035/93‑1 (MQ=255)

                                              gggTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCAt                                           >  2:3311046/1‑93 (MQ=255)

                                               ggTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCAtt                                          <  1:4445011/93‑1 (MQ=255)

                                               ggTGCGGCTGTCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCAtt                                          <  1:2491729/93‑1 (MQ=255)

                                                gTTCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTc                                         <  2:4303841/93‑1 (MQ=255)

                                                 ttCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTcc                                        <  1:2324734/93‑1 (MQ=255)

                                                 ttCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTcc                                        <  1:2386621/93‑1 (MQ=255)

                                                 ttCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTcc                                        <  2:3572992/93‑1 (MQ=255)

                                                  tCGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCa                                       <  1:1112049/93‑1 (MQ=255)

                                                   cGGCTGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAg                                      <  2:3547806/93‑1 (MQ=255)

                                                     gctggCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGc                                    <  2:1212868/90‑1 (MQ=255)

                                                        ggCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCAcc                                 >  1:2121798/1‑93 (MQ=255)

                                                         gcgcTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCa                                <  2:5996363/93‑1 (MQ=255)

                                                         gcgcTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCa                                <  1:2803920/93‑1 (MQ=255)

                                                         gcgcTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCa                                <  1:356494/93‑1 (MQ=255)

                                                          cgcTTTTTGTTCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAg                               <  1:194541/93‑1 (MQ=255)

                                                                   ttCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTaa                      >  1:4917572/1‑93 (MQ=255)

                                                                    tCGCGTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTaaa                     <  1:2951031/93‑1 (MQ=255)

                                                                     cgcgTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAAc                    <  1:1821496/93‑1 (MQ=255)

                                                                     cgcgTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAAc                    <  2:5702411/93‑1 (MQ=255)

                                                                        gTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGa                 >  2:1597501/1‑93 (MQ=255)

                                                                        gTCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGa                 <  1:4159654/93‑1 (MQ=255)

                                                                         tCCGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAg                >  2:938646/1‑93 (MQ=255)

                                                                          ccGCGTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGc               <  1:6537043/93‑1 (MQ=255)

                                                                           cgcgTATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCa              >  1:1005699/1‑93 (MQ=255)

                                                                               tATCCACCAGGTCAGCCCGAGCAATCCGTCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCAcggc          >  1:6290106/1‑93 (MQ=255)

                                                                               tATCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCAcggc          <  2:3457333/93‑1 (MQ=255)

                                                                                aTCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCAcggcg         <  2:4597/93‑1 (MQ=255)

                                                                                 tCCACCAGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCAcggcgg        >  2:2410083/1‑93 (MQ=255)

                                                                                  ccaccaGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACggcggc       >  2:4586297/1‑93 (MQ=255)

                                                                                  ccaccaGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACggcggc       <  1:5357683/93‑1 (MQ=255)

                                                                                  ccaccaGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACggcggc       >  1:5886171/1‑93 (MQ=255)

                                                                                    accaGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGgcgc     >  2:2436865/1‑93 (MQ=255)

                                                                                     ccaGGTCANCCCGAGCAATCCGGCNGNGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCt    <  1:477741/93‑1 (MQ=255)

                                                                                     ccaGGTCAGCCCGAGTAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCt    <  1:611366/93‑1 (MQ=255)

                                                                                     ccaGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCt    <  2:6200487/93‑1 (MQ=255)

                                                                                      caGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCtt   <  1:501550/93‑1 (MQ=255)

                                                                                      caGGTCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACAAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCtt   >  2:2020361/1‑93 (MQ=255)

ACGTCGCTTCGACAACGGTATTATTCTCTGCCATTTTTACCGGTTTGGGTTCGGCAGGCGCTTTTTGTTCGCGTCCGCGTATCCACCAGGCCAGCCCGAGCAATCCGGCAGTGAACAGCAAAAATACCAGGTTCGGCATTCCAGGCACCAGGCCGAGTAAACCGAGCACGGCGGCGCTTA  >  NC_000913/1962015‑1962194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`