Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,072,030 T→A C824S (TGC→AGC)  b2000 → outer membrane fluffing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,072,0300TA99.5% 746.9 / NA 205C824S (TGC→AGC) b2000outer membrane fluffing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (102/102);  minor base C (1/0);  total (103/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCG  >  NC_000913/2071941‑2072119
                                                                                         |                                                                                         
cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGcc                                                                                        <  2:6664542/93‑1 (MQ=255)
cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGcc                                                                                        <  1:3639973/93‑1 (MQ=255)
cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGcc                                                                                        <  1:4917941/93‑1 (MQ=255)
cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGcc                                                                                        <  2:1949320/93‑1 (MQ=255)
cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGcc                                                                                        >  2:2485261/1‑93 (MQ=255)
 ggggggATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt                                                                                       >  1:1029119/1‑93 (MQ=255)
 gggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt                                                                                       <  1:2245926/93‑1 (MQ=255)
 gggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt                                                                                       >  1:4947640/1‑93 (MQ=255)
 gggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCt                                                                                       >  2:4549235/1‑93 (MQ=255)
  ggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTg                                                                                      <  1:1958355/93‑1 (MQ=255)
  ggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTg                                                                                      <  2:2664531/93‑1 (MQ=255)
   gggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTgg                                                                                     <  2:4110023/93‑1 (MQ=255)
      tataTGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCg                                                                                  >  1:3664636/1‑93 (MQ=255)
      tataTGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCg                                                                                  >  1:1764224/1‑93 (MQ=255)
       atatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGCTGATGCCGGCAGCCTGGGCgg                                                                                 >  1:267639/1‑93 (MQ=255)
       atatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCgg                                                                                 >  2:3815873/1‑93 (MQ=255)
       atatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCgg                                                                                 >  1:6008209/1‑93 (MQ=255)
       atatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCgg                                                                                 <  1:396707/93‑1 (MQ=255)
        tatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCCGCCTGGGCGGa                                                                                >  2:6549642/1‑93 (MQ=255)
        tatGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGa                                                                                >  2:4166891/1‑93 (MQ=255)
            gTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATAcc                                                                            <  1:452186/93‑1 (MQ=255)
            gTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATAcc                                                                            >  1:5137123/1‑93 (MQ=255)
            gTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATAcc                                                                            <  1:3075708/93‑1 (MQ=255)
             tgctgcTGGCCATTTTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCt                                                                           <  1:1194063/93‑1 (MQ=255)
             tgctgcTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCt                                                                           <  2:5159717/93‑1 (MQ=255)
               ctgctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGa                                                                         <  1:4583662/93‑1 (MQ=255)
                 gctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAAt                                                                       >  2:137700/1‑93 (MQ=255)
                  ctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATc                                                                      >  2:39480/1‑93 (MQ=255)
                   tgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCt                                                                     >  1:357683/1‑93 (MQ=255)
                   tgGCCATTCTTCCGTTGAGGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCt                                                                     <  2:2845815/93‑1 (MQ=255)
                    ggCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTg                                                                    <  1:3191610/93‑1 (MQ=255)
                      ccATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGt                                                                  <  2:1144391/93‑1 (MQ=255)
                       cATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTa                                                                 >  2:94932/1‑93 (MQ=255)
                         ttcttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTaca                                                               >  2:694017/1‑93 (MQ=255)
                         ttcttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTaca                                                               >  1:3038103/1‑93 (MQ=255)
                          tcttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacac                                                              <  1:477483/93‑1 (MQ=255)
                           cttcCTTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacaca                                                             <  1:2854098/93‑1 (MQ=255)
                           cttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacaca                                                             >  2:1020932/1‑93 (MQ=255)
                            ttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGTCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacacac                                                            >  2:3718294/1‑93 (MQ=255)
                            ttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacacac                                                            >  2:6083878/1‑93 (MQ=255)
                            ttcCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTacacac                                                            >  2:5895192/1‑93 (MQ=255)
                               cGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtc                                                         >  1:4001146/1‑93 (MQ=255)
                                gTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCGCCCCGGCCGGATACCTCAATCTGGTACACACTtcc                                                        >  2:5678440/1‑93 (MQ=255)
                                gTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcc                                                        >  2:1566472/1‑93 (MQ=255)
                                gTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCAGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcc                                                        >  2:3407404/1‑93 (MQ=255)
                                 ttGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcct                                                       >  1:3298199/1‑93 (MQ=255)
                                 ttGATGTTAAGGATGATGACGGCTCCCGTGACGGCACGGACCGGGATGATGCCGGGAGCCTGGGCGGATACCTGAATCTGGGACACACGtcct                                                       >  1:3208955/1‑93 (MQ=255)
                                   gATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcctcc                                                     >  2:2739552/1‑93 (MQ=255)
                                    aTGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCg                                                    <  2:3275705/93‑1 (MQ=255)
                                     tGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCgg                                                   >  2:5561970/1‑93 (MQ=255)
                                     tGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCgg                                                   >  2:3087956/1‑93 (MQ=255)
                                      gTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGc                                                  <  1:581741/93‑1 (MQ=255)
                                      gTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGc                                                  >  1:5393814/1‑93 (MQ=255)
                                       ttAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGcc                                                 >  2:640015/1‑93 (MQ=255)
                                        tAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCt                                                >  1:4383181/1‑93 (MQ=255)
                                         aaGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtg                                               >  2:1933298/1‑93 (MQ=255)
                                          aGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtgt                                              >  1:4136167/1‑93 (MQ=255)
                                           ggATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTTCTCCGGCCtgcg                                             >  2:6488251/1‑91 (MQ=255)
                                           ggATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtgtg                                             >  1:67747/1‑93 (MQ=255)
                                           ggATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCtgcg                                             >  1:1954185/1‑91 (MQ=255)
                                            gatgatGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTgg                                            >  2:3020079/1‑93 (MQ=255)
                                               gatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt                                         <  2:4479703/93‑1 (MQ=255)
                                               gatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt                                         <  1:1416094/93‑1 (MQ=255)
                                               gatgaCGGCTCCCGTGACGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCt                                         >  1:3792032/1‑93 (MQ=255)
                                                    cGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACAt                                    <  1:5645355/93‑1 (MQ=255)
                                                    cGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACAt                                    >  2:3550908/1‑93 (MQ=255)
                                                      gCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtg                                  >  1:5758787/1‑93 (MQ=255)
                                                      gCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtg                                  <  2:6214282/93‑1 (MQ=255)
                                                        tCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATtgtg                                <  2:3448474/93‑1 (MQ=255)
                                                         cccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTgg                               <  2:1288203/93‑1 (MQ=255)
                                                          ccGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGc                              >  2:582364/1‑93 (MQ=255)
                                                             tGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGcaca                           >  1:157290/1‑93 (MQ=255)
                                                               ccGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAgg                         <  2:6269594/93‑1 (MQ=255)
                                                                 ggCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGa                       >  2:5728745/1‑93 (MQ=255)
                                                                 ggCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGa                       <  2:4974826/93‑1 (MQ=255)
                                                                  gCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGaa                      <  1:5588011/93‑1 (MQ=255)
                                                                  gCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGaa                      >  1:6604956/1‑93 (MQ=255)
                                                                  gCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGaa                      <  2:6037394/93‑1 (MQ=255)
                                                                   cACGGTCCGGGATGATTCCGGCAGCCTGGACGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTAGCACAGGGAAc                     <  1:4171124/93‑1 (MQ=255)
                                                                   cACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAc                     <  2:2344822/93‑1 (MQ=255)
                                                                    aCGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAcc                    >  2:4242542/1‑93 (MQ=255)
                                                                    aCGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAcc                    >  2:5398520/1‑93 (MQ=255)
                                                                     cGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAAccc                   <  2:3913236/93‑1 (MQ=255)
                                                                       gTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGc                 <  1:5175566/93‑1 (MQ=255)
                                                                        tCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGcc                >  2:4730970/1‑93 (MQ=255)
                                                                          cGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCcac              >  2:1736280/1‑93 (MQ=255)
                                                                          cGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCcac              >  1:6434042/1‑93 (MQ=255)
                                                                             gatgagGCGGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGc           <  2:1781885/93‑1 (MQ=255)
                                                                              atgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCa          <  2:2545704/93‑1 (MQ=255)
                                                                               tgatgCCGGCGGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCAt         <  1:2497649/93‑1 (MQ=255)
                                                                               tgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCAt         >  1:2117332/1‑93 (MQ=255)
                                                                               tgatgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCAt         >  1:2770955/1‑93 (MQ=255)
                                                                                 atgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGa       <  1:6291278/93‑1 (MQ=255)
                                                                                    ccGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGATATTGTGGCACAGGGAACCCGCCACAGCATGAAAg    >  1:1451232/1‑93 (MQ=255)
                                                                                    ccGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAg    <  2:1694023/93‑1 (MQ=255)
                                                                                     cGGCAGCCTGGGCGGATCCCTGAATCTGGTACACACGCCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGc   <  2:88053/93‑1 (MQ=255)
                                                                                     cGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGACCCCGCCACAGCATGAAAGc   <  1:1798689/93‑1 (MQ=255)
                                                                                     cGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGc   >  1:5354164/1‑93 (MQ=255)
                                                                                     cGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGc   >  2:4367391/1‑93 (MQ=255)
                                                                                     cGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGc   <  2:151080/93‑1 (MQ=255)
                                                                                         |                                                                                         
CGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCTGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCG  >  NC_000913/2071941‑2072119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑