BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,094,539	T→C	V225A (GTT→GCT)	hisF →	imidazole glycerol phosphate synthase holoenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,094,539	0	T	C	100.0%	819.5 / NA	251	V225A (GTT→GCT)	hisF	imidazole glycerol phosphate synthase holoenzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (101/150); total (101/150)

Only 100 of 264 total aligned reads displayed.

AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGA  >  NC_000913/2094449‑2094628

aaGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTg                                                                                         >  1:2842159/1‑93 (MQ=255)

aaGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTg                                                                                         <  2:5807986/93‑1 (MQ=255)

 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        <  2:3346127/93‑1 (MQ=255)

 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        <  1:3736585/93‑1 (MQ=255)

 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        <  1:602753/93‑1 (MQ=255)

 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        >  1:6164292/1‑93 (MQ=255)

  gTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAc                                                                                       <  1:5563304/93‑1 (MQ=255)

   tGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACg                                                                                      >  2:1802617/1‑93 (MQ=255)

   tGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACg                                                                                      >  2:1018846/1‑93 (MQ=255)

     cGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgc                                                                                    >  1:3069271/1‑93 (MQ=255)

      gTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcg                                                                                   >  1:454825/1‑93 (MQ=255)

      gTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcg                                                                                   >  2:612052/1‑93 (MQ=255)

       tGATGTTTGCCGCGTCCCTCTGATTGCCTTCTGTGGCGCGGGCTCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCTACGCTGACGgcgc                                                                                  <  1:6596564/93‑1 (MQ=255)

        gAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTGGAAGCCTTCCGCGATGCCGACGCTGCCGgggcg                                                                                 >  2:5588706/1‑93 (MQ=255)

         aaGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgcgc                                                                                <  1:5042406/93‑1 (MQ=255)

         aaGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgcgc                                                                                >  1:6342553/1‑93 (MQ=255)

         aaGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgccctc                                                                                >  1:1269665/1‑89 (MQ=255)

          aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               >  2:3468600/1‑93 (MQ=255)

          aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               >  2:2875810/1‑93 (MQ=255)

          aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               >  2:4565826/1‑93 (MQ=255)

          aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               >  2:508966/1‑93 (MQ=255)

             ttGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGc                                                                            <  2:4931521/93‑1 (MQ=255)

              tGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCa                                                                           <  2:3611996/93‑1 (MQ=255)

               gCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAg                                                                          >  2:270640/1‑93 (MQ=255)

                ccACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGc                                                                         >  1:2926772/1‑93 (MQ=255)

                 cACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCCAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCt                                                                        >  1:6516890/1‑93 (MQ=255)

                  ccGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCtt                                                                       <  2:273294/92‑1 (MQ=255)

                   cGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTc                                                                      <  2:1596990/93‑1 (MQ=255)

                   cGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTc                                                                      <  2:4506513/93‑1 (MQ=255)

                   cGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTCCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTc                                                                      <  2:2508161/93‑1 (MQ=255)

                    gTCCCGCGGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     <  1:2705531/93‑1 (MQ=255)

                     tCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCg                                                                    >  2:2135683/1‑93 (MQ=255)

                       ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCgca                                                                  >  2:5832599/1‑91 (MQ=255)

                       ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTa                                                                  >  1:1646873/1‑93 (MQ=255)

                        cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 >  2:5106198/1‑93 (MQ=255)

                        cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 >  2:3857198/1‑93 (MQ=255)

                         gCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAtt                                                                <  2:3933455/93‑1 (MQ=255)

                           tGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTcc                                                              <  1:2024944/93‑1 (MQ=255)

                            gATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCca                                                             >  1:3485510/1‑93 (MQ=255)

                             aTTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCcac                                                            <  2:6654918/93‑1 (MQ=255)

                               tGCCTCCGGTGGCGCGGGCACCATGGAACACTGCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaa                                                          <  1:690559/93‑1 (MQ=255)

                                gCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaa                                                         <  1:2661997/93‑1 (MQ=255)

                                    ccGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAcaaacaaa                                                     <  2:4996469/93‑1 (MQ=255)

                                     cGGTGGCGCGGGTACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  1:4501490/93‑1 (MQ=255)

                                     cGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  2:4656811/93‑1 (MQ=255)

                                     cGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    >  1:550192/1‑93 (MQ=255)

                                     cGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  1:5305629/93‑1 (MQ=255)

                                      ggTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaata                                                   >  2:2970828/1‑93 (MQ=255)

                                             cgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                            <  1:4212920/93‑1 (MQ=255)

                                             cgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                            <  2:810685/93‑1 (MQ=255)

                                              gggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAatat                                           <  1:1914909/93‑1 (MQ=255)

                                               ggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTTCGTATTCCACAAACAAATAATCAATAtt                                          >  1:4694705/1‑93 (MQ=255)

                                               ggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAtt                                          <  1:6422313/93‑1 (MQ=255)

                                               ggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAtt                                          <  1:5242395/93‑1 (MQ=255)

                                                 cACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTgg                                        <  1:3835375/93‑1 (MQ=255)

                                                 cACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCAACAAACAAATAATCAATATTgg                                        <  2:5667602/93‑1 (MQ=255)

                                                  aCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGt                                       >  1:1529151/1‑93 (MQ=255)

                                                  aCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGt                                       <  2:4291011/93‑1 (MQ=255)

                                                  aCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGt                                       >  1:464901/1‑93 (MQ=255)

                                                     aTGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGaa                                    <  1:2189304/93‑1 (MQ=255)

                                                      tGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAt                                   <  2:1497935/93‑1 (MQ=255)

                                                      tGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAt                                   >  1:3381561/1‑93 (MQ=255)

                                                      tGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAt                                   <  2:2206633/93‑1 (MQ=255)

                                                       ggAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAtt                                  <  1:257893/93‑1 (MQ=255)

                                                       ggAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAtt                                  >  2:4380442/1‑93 (MQ=255)

                                                        gAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTa                                 <  2:5503568/93‑1 (MQ=255)

                                                          acacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaa                               <  2:6520006/93‑1 (MQ=255)

                                                           cacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              <  1:4877062/93‑1 (MQ=255)

                                                           cacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              <  2:2097363/93‑1 (MQ=255)

                                                           cacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              <  2:226598/93‑1 (MQ=255)

                                                           cacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              <  1:2869796/93‑1 (MQ=255)

                                                            ccTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAg                             <  1:4846346/92‑1 (MQ=255)

                                                             cTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                            <  2:1646873/93‑1 (MQ=255)

                                                             cTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                            <  1:814914/93‑1 (MQ=255)

                                                                ccTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTa                         <  2:6058672/93‑1 (MQ=255)

                                                                  tCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAcc                       <  1:5204838/93‑1 (MQ=255)

                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      <  2:4015046/93‑1 (MQ=255)

                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      <  1:2361544/93‑1 (MQ=255)

                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      >  2:3256749/1‑93 (MQ=255)

                                                                    gAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTg                     <  2:899048/93‑1 (MQ=255)

                                                                     aaGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTgg                    >  1:3965208/1‑93 (MQ=255)

                                                                      aGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGc                   >  2:4183446/1‑93 (MQ=255)

                                                                       gCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCa                  >  2:1375516/1‑93 (MQ=255)

                                                                       gCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCa                  >  1:2442968/1‑93 (MQ=255)

                                                                        ccTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCaa                 >  1:740316/1‑93 (MQ=255)

                                                                          ttCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAaca               <  1:135722/93‑1 (MQ=255)

                                                                          ttCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAaca               >  2:1055923/1‑93 (MQ=255)

                                                                            ccGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAAcaca             >  2:5924305/1‑93 (MQ=255)

                                                                             cgcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAg            <  1:1006906/93‑1 (MQ=255)

                                                                              gcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAgg           >  1:4901713/1‑93 (MQ=255)

                                                                                 aTGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCg        <  2:820037/93‑1 (MQ=255)

                                                                                 aTGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCg        >  2:3083993/1‑93 (MQ=255)

                                                                                  tGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGt       <  1:1121348/93‑1 (MQ=255)

                                                                                  tGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGt       <  2:681931/93‑1 (MQ=255)

                                                                                   gCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTg      >  2:3739736/1‑93 (MQ=255)

                                                                                   gCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGCTGAATTAAAAGCGTACCTGGCAACACAGCGCGTg      >  1:1855400/1‑93 (MQ=255)

                                                                                     cGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGga    <  2:6216455/93‑1 (MQ=255)

                                                                                      gACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgag   <  2:1220817/93‑1 (MQ=255)

                                                                                      gACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgag   >  2:2041579/1‑93 (MQ=255)

                                                                                      caCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgag   <  2:4881613/92‑1 (MQ=255)

AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGA  >  NC_000913/2094449‑2094628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`