BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,151,093	A→G	C20R (TGC→CGC)	b2073 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,093	0	A	G	99.6%	748.9 / ‑4.8	230	C20R (TGC→CGC)	b2073	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (110/119); minor base T (0/1); total (110/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

Only 100 of 234 total aligned reads displayed.

CATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTC  >  NC_000913/2151003‑2151182

catcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGAc                                                                                         <  1:486303/93‑1 (MQ=255)

 atcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACg                                                                                        >  2:5627692/1‑93 (MQ=255)

   caCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTg                                                                                      >  2:645586/1‑93 (MQ=255)

    aCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTgg                                                                                     >  2:727879/1‑93 (MQ=255)

     cGAAAGGTAACCACTCCGGCTTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAATCATCCAGCAGCAAAATGCAGGGGCGACGTGGt                                                                                    <  1:4665226/93‑1 (MQ=255)

       aaaGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTc                                                                                  <  2:1643744/93‑1 (MQ=255)

        aaGGTACCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAACCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTcc                                                                                 <  1:2375480/93‑1 (MQ=255)

        aaGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTcc                                                                                 <  1:875207/93‑1 (MQ=255)

        aaGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACC‑TTCATAGAACCAGAAAAATCCAGCAGCAAAAAGCAGGGGCGACGTGGTTCCg                                                                                >  2:1438035/1‑93 (MQ=255)

         aGGTAACCAATCCGGCGTTAAGTTCCTTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCg                                                                                >  1:5254963/1‑93 (MQ=255)

          ggTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCgg                                                                               <  1:5461311/93‑1 (MQ=255)

            tAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCggct                                                                             <  1:3468090/93‑3 (MQ=255)

            tAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGt                                                                             <  1:2671998/93‑1 (MQ=255)

            tAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGt                                                                             <  2:1167947/93‑1 (MQ=255)

            tAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGt                                                                             <  1:3127868/93‑1 (MQ=255)

             aaCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGtt                                                                            >  2:5634944/1‑93 (MQ=255)

             aaCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGtt                                                                            <  2:6522817/93‑1 (MQ=255)

             aaCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGtt                                                                            >  1:3110033/1‑93 (MQ=255)

              aCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTa                                                                           >  1:1490704/1‑93 (MQ=255)

              aCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTa                                                                           >  2:5496840/1‑93 (MQ=255)

               ccAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTAc                                                                          <  1:6406864/93‑1 (MQ=255)

               ccAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTAc                                                                          <  2:4750960/93‑1 (MQ=255)

                cAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACt                                                                         <  1:3221362/93‑1 (MQ=255)

                 aaTCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTg                                                                        >  1:6646950/1‑93 (MQ=255)

                 aaTCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTg                                                                        >  2:3850003/1‑93 (MQ=255)

                  aTCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTgg                                                                       >  1:899024/1‑93 (MQ=255)

                   tCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGc                                                                      <  2:3392721/93‑1 (MQ=255)

                    ccGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCa                                                                     >  1:3996218/1‑93 (MQ=255)

                    ccGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCa                                                                     >  2:492609/1‑93 (MQ=255)

                     cGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCaa                                                                    <  2:3247983/93‑1 (MQ=255)

                        cGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAAt                                                                 >  1:991469/1‑93 (MQ=255)

                         gTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATc                                                                <  1:1690238/93‑1 (MQ=255)

                          ttAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCg                                                               >  2:5272638/1‑93 (MQ=255)

                          ttAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGAAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCg                                                               <  2:3566045/93‑1 (MQ=255)

                           tAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGc                                                              <  2:3147661/93‑1 (MQ=255)

                            aaGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCt                                                             >  2:4470710/1‑93 (MQ=255)

                              gTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtgg                                                           >  1:6250086/1‑93 (MQ=255)

                               ttCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtggt                                                          <  2:5827047/93‑1 (MQ=255)

                                tCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtggtg                                                         >  2:4847955/1‑93 (MQ=255)

                                 cATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtggtgg                                                        >  1:6582879/1‑93 (MQ=255)

                                  aTTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGc                                                       <  1:2781011/93‑1 (MQ=255)

                                  aTTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGc                                                       >  1:5750634/1‑93 (MQ=255)

                                   ttAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCa                                                      <  1:5894260/93‑1 (MQ=255)

                                   ttAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCa                                                      >  2:1679823/1‑93 (MQ=255)

                                   ttAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCa                                                      >  1:4761631/1‑93 (MQ=255)

                                   ttAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCa                                                      >  2:4945712/1‑93 (MQ=255)

                                    tAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCaa                                                     >  2:2670895/1‑93 (MQ=255)

                                     aaTAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCaaa                                                    <  1:2536779/93‑1 (MQ=255)

                                     aaTAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCaaa                                                    <  1:2913460/93‑1 (MQ=255)

                                      aTAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCATGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAAc                                                   <  2:5376163/93‑1 (MQ=255)

                                      aTAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAAc                                                   <  1:4275735/93‑1 (MQ=255)

                                        aGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGt                                                 >  2:4681216/1‑93 (MQ=255)

                                            cTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAtt                                             <  2:3501431/93‑1 (MQ=255)

                                            cTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAtt                                             <  2:394529/93‑1 (MQ=255)

                                             tACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAttt                                            <  2:5892282/93‑1 (MQ=255)

                                             tACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAttt                                            <  1:448036/93‑1 (MQ=255)

                                             tACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAttt                                            >  1:5558732/1‑93 (MQ=255)

                                               ccGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGt                                          >  1:6501607/1‑93 (MQ=255)

                                               ccGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGt                                          <  1:2169459/93‑1 (MQ=255)

                                                cGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGtt                                         <  1:3906243/93‑1 (MQ=255)

                                                 gTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTc                                        >  1:5063451/1‑93 (MQ=255)

                                                 gTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTc                                        <  2:3359415/93‑1 (MQ=255)

                                                  ttCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTct                                       >  1:4730669/1‑92 (MQ=255)

                                                  ttCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCg                                       <  2:1274618/93‑1 (MQ=255)

                                                   tCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGc                                      <  2:6271149/93‑1 (MQ=255)

                                                    cATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCt                                     <  2:5206866/93‑1 (MQ=255)

                                                     aTAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTc                                    >  1:2435210/1‑93 (MQ=255)

                                                       aGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCAt                                  <  2:363218/93‑1 (MQ=255)

                                                        gAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATa                                 >  1:3795290/1‑93 (MQ=255)

                                                        gAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATa                                 >  1:2934175/1‑93 (MQ=255)

                                                         aaCCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATaa                                >  2:5087290/1‑93 (MQ=255)

                                                          aCCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAg                               <  2:3135657/93‑1 (MQ=255)

                                                             aGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGaaa                            <  1:2093389/93‑1 (MQ=255)

                                                             aGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGaaa                            <  2:2322097/93‑1 (MQ=255)

                                                             aGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGCGATTTGTTCGCTCATAAGaaa                            >  1:3516619/1‑93 (MQ=255)

                                                                aaCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATcc                         <  2:6073886/93‑1 (MQ=255)

                                                                aaCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATcc                         >  2:1351645/1‑93 (MQ=255)

                                                                aaCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATcc                         >  1:4150463/1‑93 (MQ=255)

                                                                aaCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATcc                         >  2:1593394/1‑93 (MQ=255)

                                                                aaCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATcc                         <  1:3313150/93‑1 (MQ=255)

                                                                 aCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCt                        <  1:5501997/93‑1 (MQ=255)

                                                                 aCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCt                        <  2:216791/93‑1 (MQ=255)

                                                                  cATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCtt                       <  1:3606179/93‑1 (MQ=255)

                                                                    tCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTc                     >  2:1789175/1‑93 (MQ=255)

                                                                       agcagcAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTa                  <  2:3544654/93‑1 (MQ=255)

                                                                        gcagcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTag                 >  2:1972439/1‑93 (MQ=255)

                                                                        gcagcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTag                 >  2:4137078/1‑93 (MQ=255)

                                                                         cagcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTaga                <  1:166510/93‑1 (MQ=255)

                                                                           gcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAgaga              <  1:5359252/93‑1 (MQ=255)

                                                                           gcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAgaga              <  1:1944368/93‑1 (MQ=255)

                                                                           gcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAgaga              >  1:1227413/1‑93 (MQ=255)

                                                                            caAAATGCAGGGGCTACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGaa             <  1:1340961/93‑1 (MQ=255)

                                                                               aaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTa          <  2:5053837/93‑1 (MQ=255)

                                                                                  gCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCg       <  2:544370/93‑1 (MQ=255)

                                                                                  gCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCg       >  1:6317400/1‑93 (MQ=255)

                                                                                   cAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGa      <  2:674104/93‑1 (MQ=255)

                                                                                    aGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAg     <  1:5187663/93‑1 (MQ=255)

                                                                                     ggggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAgg    >  2:2864361/1‑93 (MQ=255)

                                                                                     ggggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAgg    >  1:1068363/1‑93 (MQ=255)

                                                                                       ggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTc  <  1:743647/93‑1 (MQ=255)

CATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTC  >  NC_000913/2151003‑2151182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`