BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,163,496	T→C	S96P (TCC→CCC)	b2080 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,163,496	0	T	C	99.6%	812.8 / ‑4.6	248	S96P (TCC→CCC)	b2080	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (153/94); minor base A (0/1); total (153/95)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.16e-01

Only 100 of 262 total aligned reads displayed.

TTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTA  >  NC_000913/2163406‑2163583

ttCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCcccc                                                                                       <  2:6165423/93‑1 (MQ=255)

  cTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGt                                                                                     >  1:5251558/1‑93 (MQ=255)

  cTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGt                                                                                     <  1:3133261/93‑1 (MQ=255)

   tATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGtt                                                                                    <  2:5221707/93‑1 (MQ=255)

    aTTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTa                                                                                   >  2:1158772/1‑93 (MQ=255)

     tttCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTaa                                                                                  >  2:495108/1‑93 (MQ=255)

          aTCTGAAAGCCGCTAATCACCAAATTATCGGCTCCAGGCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTTCCCCCGGTTAAGCCa                                                                             >  1:6563827/1‑93 (MQ=255)

           tCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCaa                                                                            >  1:6259915/1‑93 (MQ=255)

           tCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCaa                                                                            >  2:247005/1‑93 (MQ=255)

           tCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCaa                                                                            <  1:5036311/93‑1 (MQ=255)

            cTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAAt                                                                           <  1:3039624/93‑1 (MQ=255)

            cTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAAt                                                                           <  2:3809229/93‑1 (MQ=255)

             tGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATg                                                                          <  2:4500456/93‑1 (MQ=255)

             tGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATg                                                                          >  1:4696916/1‑93 (MQ=255)

             tGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATg                                                                          >  1:453029/1‑93 (MQ=255)

             tGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATg                                                                          >  1:3888358/1‑93 (MQ=255)

              gAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATgg                                                                         >  2:3940763/1‑93 (MQ=255)

              gAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATgg                                                                         >  2:273797/1‑93 (MQ=255)

                aaGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGca                                                                       >  2:6335605/1‑93 (MQ=255)

                 aGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGcac                                                                      <  1:3244067/93‑1 (MQ=255)

                 aGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGcac                                                                      >  1:4277600/1‑93 (MQ=255)

                  gCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGcacc                                                                     >  2:1278494/1‑92 (MQ=255)

                  gCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGcaca                                                                     >  2:6414770/1‑93 (MQ=255)

                     gCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGc                                                                  >  1:5358786/1‑93 (MQ=255)

                     gCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGc                                                                  <  1:6404321/93‑1 (MQ=255)

                     gCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGc                                                                  >  2:4574832/1‑93 (MQ=255)

                        aaTCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAg                                                               >  1:1484974/1‑93 (MQ=255)

                        aaTCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAg                                                               >  1:5597452/1‑93 (MQ=255)

                          tcatcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGAc                                                             <  2:1325945/93‑1 (MQ=255)

                          tcatcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGAc                                                             >  1:3678299/1‑93 (MQ=255)

                           catcaAATTATCGGCTCCAGCCAGATGTACGACACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACg                                                            >  1:3588443/1‑93 (MQ=255)

                            atcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACgg                                                           >  1:5004068/1‑93 (MQ=255)

                            atcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACgg                                                           <  2:3058845/93‑1 (MQ=255)

                            atcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACgg                                                           <  1:2814242/93‑1 (MQ=255)

                             tcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGt                                                          >  2:1764609/1‑93 (MQ=255)

                             tcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGt                                                          <  2:6570764/93‑1 (MQ=255)

                               aaaTTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGa                                                        <  1:4831450/93‑1 (MQ=255)

                               aaaTTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGa                                                        >  2:4613304/1‑93 (MQ=255)

                                 aTTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGaaa                                                      >  1:4020940/1‑93 (MQ=255)

                                 aTTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGaaa                                                      >  1:3716471/1‑93 (MQ=255)

                                  ttATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAg                                                     >  2:872423/1‑93 (MQ=255)

                                   tATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGa                                                    >  2:6730933/1‑93 (MQ=255)

                                    aTCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGAc                                                   <  2:3675787/93‑1 (MQ=255)

                                    aTCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGAc                                                   >  1:1788964/1‑93 (MQ=255)

                                     tCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACa                                                  <  1:4506570/93‑1 (MQ=255)

                                       ggCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAAt                                                <  1:2939122/93‑1 (MQ=255)

                                        gCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATa                                               >  1:6698879/1‑93 (MQ=255)

                                        gCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATa                                               <  1:5986816/93‑1 (MQ=255)

                                        gCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATa                                               <  2:5320409/93‑1 (MQ=255)

                                          tCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACg                                             >  1:1534258/1‑93 (MQ=255)

                                           ccagccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGt                                            >  1:3914679/1‑93 (MQ=255)

                                            cagccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTa                                           <  2:5514011/93‑1 (MQ=255)

                                              gccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAAt                                         <  1:4714424/93‑1 (MQ=255)

                                              gccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAAt                                         <  2:4842833/93‑1 (MQ=255)

                                                cagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTa                                       >  2:5251926/1‑93 (MQ=255)

                                                cagATGTACGCCACCGCGCAATCTCGTGAAACCAGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTa                                       <  1:2131175/93‑1 (MQ=255)

                                                 agATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTAc                                      <  1:1050637/93‑1 (MQ=255)

                                                  gATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACg                                     >  1:6183775/1‑93 (MQ=255)

                                                  gATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACg                                     <  1:23532/93‑1 (MQ=255)

                                                   aTGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGa                                    <  1:570546/93‑1 (MQ=255)

                                                   aTGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGa                                    >  2:2405845/1‑93 (MQ=255)

                                                   aTGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGa                                    >  1:3807746/1‑93 (MQ=255)

                                                    tGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGAt                                   <  1:4635269/93‑1 (MQ=255)

                                                    tGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGAt                                   >  2:2068964/1‑93 (MQ=255)

                                                      tACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGc                                 <  1:1377284/93‑1 (MQ=255)

                                                      tACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGc                                 <  2:5833924/93‑1 (MQ=255)

                                                       aCGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGcc                                <  2:21109/93‑1 (MQ=255)

                                                        cGCCACCGCGCAATCTCGTGAAACGGCAATTCCCCCCGTTAAAGCAAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCg                               <  1:851239/93‑1 (MQ=255)

                                                        cGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCg                               <  2:1509527/93‑1 (MQ=255)

                                                         gCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCgg                              >  1:271762/1‑93 (MQ=255)

                                                         gCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCgg                              <  1:5221336/93‑1 (MQ=255)

                                                            aCCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCg                           >  2:2641439/1‑93 (MQ=255)

                                                            aCCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCg                           >  2:1571341/1‑93 (MQ=255)

                                                            aCCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCg                           >  1:1696966/1‑93 (MQ=255)

                                                            aCCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCg                           <  1:4719125/93‑1 (MQ=255)

                                                              cgcgCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTg                         <  2:2981835/93‑1 (MQ=255)

                                                               gcgcAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTgg                        <  2:351980/93‑1 (MQ=255)

                                                               gcgcAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTgg                        <  2:4334399/93‑1 (MQ=255)

                                                                 gcAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgt                      >  2:2366184/1‑93 (MQ=255)

                                                                  cAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgtt                     >  1:1034668/1‑93 (MQ=255)

                                                                    aTCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgttgt                   >  2:1007828/1‑93 (MQ=255)

                                                                    aTCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgttgt                   <  2:5057307/93‑1 (MQ=255)

                                                                     tctcGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgttgtt                  >  2:3596174/1‑93 (MQ=255)

                                                                     tctcGTAAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgttgtt                  <  1:3416867/93‑1 (MQ=255)

                                                                      ctcGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGttgttg                 <  1:4352236/93‑1 (MQ=255)

                                                                       tcGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTgg                >  2:5139684/1‑93 (MQ=255)

                                                                       tcGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTgg                >  2:4047497/1‑93 (MQ=255)

                                                                       tcGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTgg                <  2:37256/93‑1 (MQ=255)

                                                                       tcGTGAAACCGGAATTCCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTgg                <  2:454244/93‑1 (MQ=255)

                                                                            aaaCCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTcc           <  1:906892/93‑1 (MQ=255)

                                                                            aaaCCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTcc           <  2:6509830/93‑1 (MQ=255)

                                                                             acccGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACTTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCg          <  2:2220396/91‑1 (MQ=255)

                                                                              aCCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCgg         >  2:5737031/1‑93 (MQ=255)

                                                                               ccGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGgc        >  1:4226128/1‑93 (MQ=255)

                                                                               ccGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGgc        <  2:6594623/93‑1 (MQ=255)

                                                                                 ggAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGgcgc      >  2:1782990/1‑93 (MQ=255)

                                                                                 ggAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGgcgc      <  2:4218020/93‑1 (MQ=255)

                                                                                 ggAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGgcgc      >  1:2010498/1‑93 (MQ=255)

                                                                                    aTTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCttt   >  2:6714594/1‑93 (MQ=255)

                                                                                    aTTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCttt   >  2:4894050/1‑93 (MQ=255)

TTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTA  >  NC_000913/2163406‑2163583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`