BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,265,885	A→G	S13G (AGC→GGC)	yeiR →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,265,885	0	A	G	99.5%	649.1 / ‑6.8	201	S13G (AGC→GGC)	yeiR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (104/96); total (104/97)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

TTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTC  >  NC_000913/2265795‑2265974

ttttttAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCggcggc                                                                                         <  2:6247310/93‑1 (MQ=255)

ttttttAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCggcggc                                                                                         >  1:493180/1‑93 (MQ=255)

 tttttAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGgcggcg                                                                                        >  1:4250933/1‑93 (MQ=255)

      aGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGaaa                                                                                   >  2:4259688/1‑93 (MQ=255)

       gCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGaaaa                                                                                  >  2:3655400/1‑93 (MQ=255)

       gCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGaaaa                                                                                  >  2:3085938/1‑93 (MQ=255)

       gCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGaaaa                                                                                  <  2:5484100/93‑1 (MQ=255)

       gCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGCCGGCGGGaaaa                                                                                  >  2:1387671/1‑93 (MQ=255)

         cAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAAcc                                                                                >  2:3245240/1‑93 (MQ=255)

         cAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAAcc                                                                                >  1:4914761/1‑93 (MQ=255)

          aGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCa                                                                               >  2:2581634/1‑93 (MQ=255)

            gATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACg                                                                             >  1:1021003/1‑93 (MQ=255)

            gATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGCTTTCTCGGCGGAGGGCAAcccccc                                                                             >  1:805255/1‑90 (MQ=255)

             aTTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCCCCGGTTTTCTCGGCGGCGGGAAAACCACGt                                                                            <  1:5277682/93‑1 (MQ=255)

              ttAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTc                                                                           >  2:5264839/1‑93 (MQ=255)

               tAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCg                                                                          >  2:6175958/1‑93 (MQ=255)

               tAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCg                                                                          <  2:5309954/93‑1 (MQ=255)

               tAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCg                                                                          <  2:2020877/93‑1 (MQ=255)

                aaCGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGa                                                                         >  2:4493942/1‑93 (MQ=255)

                 aCGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAt                                                                        >  1:1437705/1‑93 (MQ=255)

                 aCGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAt                                                                        >  1:4669944/1‑93 (MQ=255)

                   gCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAttc                                                                      >  1:2314239/1‑93 (MQ=255)

                   gCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAttc                                                                      >  1:5484528/1‑93 (MQ=255)

                      cAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAttcttc                                                                   >  2:1726824/1‑93 (MQ=255)

                      cAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAttcttc                                                                   >  1:86199/1‑93 (MQ=255)

                      cAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGAttcttc                                                                   >  1:3980951/1‑93 (MQ=255)

                       aGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCa                                                                  <  1:318261/93‑1 (MQ=255)

                        gTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCAt                                                                 >  2:176609/1‑93 (MQ=255)

                         ttAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATc                                                                <  2:6020736/93‑1 (MQ=255)

                         ttAACTAACCGGAGTATTGCGGAGCCGTTGTGACAGGGACCAACCTCATCACCGTTTTTCTGGGGGGCGGGAAAACCACGTCGATTCTTCATc                                                                <  2:1269388/93‑1 (MQ=255)

                            aCTTCCCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGt                                                             <  2:3686822/93‑1 (MQ=255)

                            aCTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGt                                                             <  1:1353263/93‑1 (MQ=255)

                            aCTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGt                                                             <  2:436101/93‑1 (MQ=255)

                             cTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGtt                                                            >  2:2047138/1‑93 (MQ=255)

                             cTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGtt                                                            >  2:4828688/1‑93 (MQ=255)

                              ttACCAGAGAATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTa                                                           >  1:6092786/1‑93 (MQ=255)

                               tACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCCCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAg                                                          <  1:499240/93‑1 (MQ=255)

                               tACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAg                                                          <  1:6162344/93‑1 (MQ=255)

                               tACCAGAGTATGGCGGAGCCGTTGTGACCAGGACCACCCTCATCCCGGGTTTTCTGGGCGGCGGAAAAACCACGTCGATTCTTCATCTGTTAg                                                          <  2:4560028/93‑1 (MQ=255)

                                 ccAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGcc                                                        <  1:2723630/93‑1 (MQ=255)

                                 ccAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGcc                                                        >  1:6111203/1‑93 (MQ=255)

                                  cAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGccc                                                       >  2:240119/1‑93 (MQ=255)

                                   agagTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCa                                                      >  2:5926899/1‑93 (MQ=255)

                                   agagTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCa                                                      >  1:1453319/1‑93 (MQ=255)

                                    gagTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCAt                                                     <  2:5022578/93‑1 (MQ=255)

                                     agTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATa                                                    <  1:2246812/93‑1 (MQ=255)

                                         ttGCGGAGCCGTTGTGACCAGGACCACCCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGa                                                <  2:3744737/93‑1 (MQ=255)

                                         ttGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGa                                                <  1:2846435/93‑1 (MQ=255)

                                         ttGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGa                                                <  1:6370309/93‑1 (MQ=255)

                                             ggAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATccc                                            <  2:2965245/93‑1 (MQ=255)

                                             ggAGCCGTTCTGACCAGGACCACCCTCATCACCGTTTTTCGCGGCGTGAGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATccc                                            <  1:3745667/93‑1 (MQ=255)

                                                gCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAAc                                         >  2:3164068/1‑93 (MQ=255)

                                                 ccGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACg                                        <  2:2289554/93‑1 (MQ=255)

                                                  cGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGa                                       <  1:3020643/93‑1 (MQ=255)

                                                   gTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaa                                      >  1:2210004/1‑93 (MQ=255)

                                                      gtgACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaaa                                   >  1:574095/1‑93 (MQ=255)

                                                      atgACAAGGCCCAACCTCACCACCGCTTGTCTCTGCGGCGGGAAAACCACGTAGATGCTTCATCTGTTAGCCCATAAAGATCCCAACGaaaaa                                   <  1:1093455/92‑1 (MQ=255)

                                                       tgACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAAt                                  >  2:4630043/1‑93 (MQ=255)

                                                       tgACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAAt                                  <  1:5203366/93‑1 (MQ=255)

                                                        gACCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATg                                 <  2:3434967/93‑1 (MQ=255)

                                                         aCCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATgg                                >  1:330256/1‑93 (MQ=255)

                                                         aCCAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATgg                                <  1:6670463/93‑1 (MQ=255)

                                                           cAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGc                              >  2:3928808/1‑93 (MQ=255)

                                                           cAGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGc                              <  1:5823536/93‑1 (MQ=255)

                                                            aGGACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCg                             <  2:1536263/93‑1 (MQ=255)

                                                             ggACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCgg                            >  2:6555197/1‑93 (MQ=255)

                                                             ggACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCgg                            >  2:238844/1‑93 (MQ=255)

                                                             ggACCAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCgg                            >  2:5850378/1‑93 (MQ=255)

                                                              gcccAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGt                           <  1:1568992/91‑1 (MQ=255)

                                                                ccACCCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTAc                         <  1:4736391/93‑1 (MQ=255)

                                                                 cAACCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACt                        <  1:1601484/93‑1 (MQ=255)

                                                                   aCCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTgg                      >  1:2551626/1‑93 (MQ=255)

                                                                   aCCTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTgg                      >  1:627033/1‑93 (MQ=255)

                                                                    ccTCATCACCGGTTTTCTCGGCGGCGGGAAACCCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGt                     <  1:3543309/93‑1 (MQ=255)

                                                                     cTCATCACCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGtt                    >  2:6615282/1‑93 (MQ=255)

                                                                     cTCATCACCGGTTTTCTCGGCGGCGGAAAACCCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGtt                    <  2:42543/93‑1 (MQ=255)

                                                                      tcatcaCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTa                   >  2:5758314/1‑93 (MQ=255)

                                                                        atcaCCGTTTTGCTCGGCGGCGGAAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAAt                 <  1:3410470/93‑1 (MQ=255)

                                                                        atcaCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAAt                 <  1:3327183/93‑1 (MQ=255)

                                                                        atcaCCGGTTTCCTCGTCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAAt                 <  1:6704045/93‑1 (MQ=255)

                                                                          caCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGa               <  1:1068475/93‑1 (MQ=255)

                                                                           aCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGaa              >  1:437278/1‑93 (MQ=255)

                                                                           aCCGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGaa              >  1:1925018/1‑93 (MQ=255)

                                                                           aCCGGTATTCTCGGCGCCGGGAAAGCCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGaa              <  1:6022363/93‑1 (MQ=255)

                                                                            ccGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAAt             <  1:2687237/93‑1 (MQ=255)

                                                                             cGGTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAAtt            <  1:3085938/93‑1 (MQ=255)

                                                                              ggTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAAttt           >  1:5037622/1‑93 (MQ=255)

                                                                              gcttttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAAttt           <  1:3661152/91‑1 (MQ=255)

                                                                               gTTTTCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTg          >  2:6346085/1‑93 (MQ=255)

                                                                                ttttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgg         >  2:4883556/1‑93 (MQ=255)

                                                                                ttttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgg         >  2:4913565/1‑93 (MQ=255)

                                                                                ttttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgg         >  1:3100819/1‑93 (MQ=255)

                                                                                 tttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTggg        <  2:649665/93‑1 (MQ=255)

                                                                                  ttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgggg       <  2:2750572/93‑1 (MQ=255)

                                                                                  ttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgggg       <  1:4874639/93‑1 (MQ=255)

                                                                                  ttCTCGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTgggg       >  1:4018627/1‑93 (MQ=255)

                                                                                   tctcGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGa      <  1:6297072/93‑1 (MQ=255)

                                                                                   tctcGGCGGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGa      <  1:5119628/93‑1 (MQ=255)

                                                                                      cggcggcggGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGt   <  1:1283257/93‑1 (MQ=255)

                                                                                       ggcggcGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTc  <  1:1755584/93‑1 (MQ=255)

TTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTAGCCCATAAAGATCCCAACGAAAAATGGGCGGTACTGGTTAATGAATTTGGGGAAGTC  >  NC_000913/2265795‑2265974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`