BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,325,759	A→G	G135G (GGT→GGC)	b2225 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,325,759	0	A	G	99.6%	770.8 / ‑5.1	239	G135G (GGT→GGC)	b2225	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (107/131); total (108/131)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

Only 100 of 252 total aligned reads displayed.

GCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAGTGAAAATATA  >  NC_000913/2325669‑2325848

gCCATCGGCCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGcc                                                                                         <  2:2672193/93‑1 (MQ=255)

 ccATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGccc                                                                                        <  1:4753887/93‑1 (MQ=255)

 ccATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGccc                                                                                        >  1:1322022/1‑93 (MQ=255)

 ccATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGccc                                                                                        <  2:4166925/93‑1 (MQ=255)

 acagcGGACGAAACAACGTGAGGGTAGAGGTCGGTATTGTCCGTACCGGCAAAGAGAACGACCCGTAAACGTGCACGAATCGTTCCTGTGccc                                                                                        <  2:3516565/89‑1 (MQ=255)

  cATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCg                                                                                       <  2:3204793/93‑1 (MQ=255)

   aTCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCTCGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCgg                                                                                      <  2:4324802/93‑1 (MQ=255)

    tCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGc                                                                                     >  2:51197/1‑93 (MQ=255)

      gtgCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGACCCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGt                                                                                   <  2:2394273/91‑1 (MQ=255)

      gggCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGt                                                                                   <  2:6639769/93‑1 (MQ=255)

      gggCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGt                                                                                   >  2:2519020/1‑93 (MQ=255)

       ggCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGtt                                                                                  <  1:4154263/93‑1 (MQ=255)

       ggCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGtt                                                                                  >  2:1403623/1‑93 (MQ=255)

       ggCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGtt                                                                                  <  2:892402/93‑1 (MQ=255)

         cGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTACCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGa                                                                                <  1:2937570/93‑1 (MQ=255)

          gTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAg                                                                               <  2:2906450/93‑1 (MQ=255)

            aacaacGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTa                                                                             >  1:1519179/1‑93 (MQ=255)

            aacaacGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTa                                                                             >  1:3127012/1‑93 (MQ=255)

            aacaacGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTa                                                                             >  2:3366505/1‑93 (MQ=255)

             acaacGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTaa                                                                            >  2:876378/1‑93 (MQ=255)

              caacGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGACGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTaaa                                                                           >  1:6623253/1‑93 (MQ=255)

                  gTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTg                                                                       >  2:4500452/1‑93 (MQ=255)

                  gTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTg                                                                       <  2:1581005/93‑1 (MQ=255)

                      aggtcgaggtcgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCAtt                                                                   >  2:5552018/1‑93 (MQ=255)

                      aggtcgaggtcgGTATTGTCCGTACCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCAtt                                                                   <  2:6346919/93‑1 (MQ=255)

                       ggtggaggtcgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCAttt                                                                  <  2:1169755/93‑1 (MQ=255)

                       ggtcgaggtcgGTATTGTCCGTATCCCACGAGAGACCCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCAttt                                                                  <  1:1999474/93‑1 (MQ=255)

                       ggtcgaggtcgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCAttt                                                                  <  1:3212922/93‑1 (MQ=255)

                         tcgaggtcgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCAttttt                                                                <  2:822924/93‑1 (MQ=255)

                         tcgaggtcgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCAttttt                                                                <  2:4799952/93‑1 (MQ=255)

                           gaggtcgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTg                                                              <  1:999684/93‑1 (MQ=255)

                            aggtcgGTATTGTCCGTATCCCACGAGAGACCCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGt                                                             <  1:6688712/93‑1 (MQ=255)

                            aggtcgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGt                                                             <  1:2443624/93‑1 (MQ=255)

                             ggtcgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTc                                                            <  1:3279352/93‑1 (MQ=255)

                             ggtcgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTc                                                            <  1:208817/93‑1 (MQ=255)

                             cgtcgCTATTTTCCGTATCCCACGAGAGATCCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTc                                                            <  1:1176045/92‑1 (MQ=255)

                                cgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGc                                                         <  1:2687100/93‑1 (MQ=255)

                                cgGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGc                                                         <  2:4421420/93‑1 (MQ=255)

                                  gTATTGTCCGTGTCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCtt                                                       <  1:3996248/93‑1 (MQ=255)

                                  gTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCtt                                                       >  1:4813988/1‑93 (MQ=255)

                                  gTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCtt                                                       >  2:152428/1‑93 (MQ=255)

                                  gTATTGTCCGTACCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCtt                                                       <  1:5912343/93‑1 (MQ=255)

                                   tATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCttt                                                      <  1:5077529/93‑1 (MQ=255)

                                    aTTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAACACTGCATTTTTTGTCGGCTTTg                                                     >  2:5376618/1‑93 (MQ=255)

                                    aTTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTg                                                     >  2:5266037/1‑93 (MQ=255)

                                      tGTCCGTATCCCACGAGAGACCCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGcc                                                   <  1:4647677/93‑1 (MQ=255)

                                      tGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGcc                                                   <  2:2322351/93‑1 (MQ=255)

                                        tCCTTACCCCACGAGAGACCCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCa                                                 <  1:2332916/93‑1 (MQ=255)

                                         ccGTACCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCAt                                                <  1:4570250/93‑1 (MQ=255)

                                           gTATCCCACGAGAGACCCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATcc                                              <  2:6605193/93‑1 (MQ=255)

                                           gTATCCCACGAGAGAACCAGCCGTAAACGTGCGCGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATcc                                              <  1:431275/93‑1 (MQ=255)

                                           gTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATcc                                              >  2:3112197/1‑93 (MQ=255)

                                           gTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATcc                                              <  2:1932692/93‑1 (MQ=255)

                                           gTAGCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATcc                                              <  1:172443/93‑1 (MQ=255)

                                            tATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCg                                             <  1:3017878/93‑1 (MQ=255)

                                             aTCCCACGAGAGACCCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCgg                                            <  1:71290/93‑1 (MQ=255)

                                              tCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCggg                                           >  1:2134874/1‑93 (MQ=255)

                                               cccACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGc                                          <  2:3933480/93‑1 (MQ=255)

                                                ccACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCt                                         >  2:6337777/1‑93 (MQ=255)

                                                   cGAGAGACCCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCtgat                                      <  2:4845479/93‑1 (MQ=255)

                                                   cGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCtgat                                      <  2:4412830/93‑1 (MQ=255)

                                                   cGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCtgat                                      <  1:89177/93‑1 (MQ=255)

                                                       agaACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGAcc                                  <  2:2105971/93‑1 (MQ=255)

                                                        gaACCAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCt                                 <  1:4417847/93‑1 (MQ=255)

                                                           ccAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCa                              <  1:4792956/93‑1 (MQ=255)

                                                           ccAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCa                              >  1:5635807/1‑93 (MQ=255)

                                                            cAGCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCAc                             >  1:2821998/1‑93 (MQ=255)

                                                              gcagTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGc                           <  2:922158/90‑1 (MQ=255)

                                                              gCCGTAAACGTGCACGAATCTTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGACTTTGCCCATCCGGGCTGATGACCTGCACga                           <  1:2750349/93‑2 (MQ=255)

                                                              gCCGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGc                           >  1:1632492/1‑93 (MQ=255)

                                                                cGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTg                         >  2:3883141/1‑93 (MQ=255)

                                                                cGTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTg                         >  2:1988724/1‑93 (MQ=255)

                                                                 gTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGt                        <  2:2231354/93‑1 (MQ=255)

                                                                 gTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGt                        >  2:6479435/1‑93 (MQ=255)

                                                                 gTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGt                        <  1:4294227/93‑1 (MQ=255)

                                                                 gTAAACGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGt                        <  2:2065189/93‑1 (MQ=255)

                                                                   aaaCGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTtg                      >  1:538022/1‑93 (MQ=255)

                                                                   aaaCGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTtg                      >  1:2101409/1‑93 (MQ=255)

                                                                    aaCGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTtgc                     >  1:4991148/1‑93 (MQ=255)

                                                                    aaCGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTtgc                     >  1:1945265/1‑93 (MQ=255)

                                                                    aaCGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTtgc                     <  2:2862096/93‑1 (MQ=255)

                                                                    aaCGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTtgc                     >  1:6053852/1‑93 (MQ=255)

                                                                    aaCGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTtgc                     <  1:6304714/93‑1 (MQ=255)

                                                                    aaCGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTtgc                     <  2:6460055/93‑1 (MQ=255)

                                                                      cGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTtgctg                   >  1:5564831/1‑93 (MQ=255)

                                                                      cGTGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTtgctg                   <  1:6482618/93‑1 (MQ=255)

                                                                        tGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGcc                 >  1:1031795/1‑93 (MQ=255)

                                                                        tGCACGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGcc                 <  2:3611404/93‑1 (MQ=255)

                                                                           aCGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTc              >  2:2363976/1‑93 (MQ=255)

                                                                           aCGAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTc              >  2:6192580/1‑93 (MQ=255)

                                                                             gAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAg            <  2:691144/93‑1 (MQ=255)

                                                                             gAATCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAg            >  1:1801955/1‑93 (MQ=255)

                                                                              aaTCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAGt           >  1:2624058/1‑93 (MQ=255)

                                                                              aaTCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAGt           <  2:4631351/93‑1 (MQ=255)

                                                                               aTCGTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAGTg          <  1:5283552/93‑1 (MQ=255)

                                                                                  gTTCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAGTGaaa       >  1:3883670/1‑93 (MQ=255)

                                                                                   ttCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAGTGaaaa      >  1:3375222/1‑93 (MQ=255)

                                                                                   ttCCTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAGTGaaaa      >  1:5522882/1‑93 (MQ=255)

                                                                                    tCCTGTGCCCGGCGTTAAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAGTGAAAat     <  1:3596006/93‑1 (MQ=255)

                                                                                     ccTGTGCCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAGTGAAAata    <  2:598452/93‑1 (MQ=255)

GCCATCGGGCGTAACAACGTGAAGGTCGAGGTCGGTATTGTCCGTATCCCACGAGAGAACCAGCCGTAAACGTGCACGAATCGTTCCTGTACCCGGCGTTGAGTAAAACTGCATTTTTTGTCGGCTTTGCCCATCCGGGCTGATGACCTGCACGCTGTTGCTGCCTTCAGTGAAAATATA  >  NC_000913/2325669‑2325848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`