BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,362,769	G→A	E66K (GAA→AAA)	yfaO →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,362,769	0	G	A	99.6%	911.5 / ‑5.3	244	E66K (GAA→AAA)	yfaO	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (117/126); total (117/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 251 total aligned reads displayed.

TCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT  >  NC_000913/2362679‑2362858

    gTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCa                                                                                     <  1:6037344/93‑1 (MQ=255)

     tGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCAc                                                                                    <  1:1971882/93‑1 (MQ=255)

       gcggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  2:665898/93‑1 (MQ=255)

       gcggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  1:466138/93‑1 (MQ=255)

       gcggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  2:2663571/93‑1 (MQ=255)

       gcggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  2:1775786/93‑1 (MQ=255)

        cggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGcc                                                                                 <  1:1151342/93‑1 (MQ=255)

        cggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGcc                                                                                 <  2:6549567/93‑1 (MQ=255)

          gcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGt                                                                               >  1:5395079/1‑93 (MQ=255)

            gTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAAATGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTgg                                                                             >  2:1190242/1‑93 (MQ=255)

             tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            >  2:3927955/1‑93 (MQ=255)

             tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            <  2:2196929/93‑1 (MQ=255)

              ggAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAc                                                                           <  2:2293470/93‑1 (MQ=255)

              ggAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAc                                                                           >  1:5765240/1‑93 (MQ=255)

               gAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAcc                                                                          >  2:862200/1‑93 (MQ=255)

               gAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAcc                                                                          <  2:3667090/93‑1 (MQ=255)

               gAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAcc                                                                          <  2:5943743/93‑1 (MQ=255)

               gAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAcc                                                                          >  1:4089224/1‑93 (MQ=255)

                aGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCt                                                                         <  2:2723405/93‑1 (MQ=255)

                aGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCt                                                                         <  1:4311216/93‑1 (MQ=255)

                  ccTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTc                                                                       <  2:3795951/93‑1 (MQ=255)

                  ccTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTc                                                                       <  1:1580059/93‑1 (MQ=255)

                    tGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAg                                                                     >  2:5375920/1‑93 (MQ=255)

                    tGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAg                                                                     <  1:2645729/93‑1 (MQ=255)

                     ggCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGACCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGc                                                                    >  2:1446769/1‑93 (MQ=255)

                      gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCg                                                                   <  2:3411298/93‑1 (MQ=255)

                       cgaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCga                                                                  <  2:2014982/93‑1 (MQ=255)

                        gaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgat                                                                 <  1:4025528/93‑1 (MQ=255)

                        gaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgat                                                                 >  1:481294/1‑93 (MQ=255)

                           cgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgatgat                                                              <  1:4277858/93‑1 (MQ=255)

                            gaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGata                                                             <  1:5107730/93‑1 (MQ=255)

                            gaaTTCAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGata                                                             >  1:4332532/1‑93 (MQ=255)

                              aTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAAGAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAtt                                                           <  2:4997646/93‑1 (MQ=255)

                               ttGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTc                                                          >  2:2534180/1‑93 (MQ=255)

                                 gAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGc                                                        >  1:1459298/1‑93 (MQ=255)

                                 gAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGc                                                        <  2:3839339/93‑1 (MQ=255)

                                  aaGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCa                                                       >  2:3136751/1‑93 (MQ=255)

                                   agagGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAc                                                      >  1:2716695/1‑93 (MQ=255)

                                    gagGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAcc                                                     >  1:5176704/1‑93 (MQ=255)

                                      ggCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCaa                                                   >  1:3749793/1‑93 (MQ=255)

                                      ggCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCaa                                                   <  1:3238909/93‑1 (MQ=255)

                                       gCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAg                                                  <  1:351385/93‑1 (MQ=255)

                                         aCTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAc                                                >  1:6462700/1‑93 (MQ=255)

                                         aCTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAc                                                >  2:4407511/1‑93 (MQ=255)

                                           tACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGt                                              <  2:2477115/93‑1 (MQ=255)

                                            aCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTa                                             >  2:1869856/1‑93 (MQ=255)

                                             cgccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTAt                                            <  1:3086168/93‑1 (MQ=255)

                                              gccgcGAAATTCGCGAAGAGCTGGAAGAACAGCTGCTTTTGACAAAAATCCCGCCGTAGACCTTCAGCGATGATATTCGCACCAAGGCGTATg                                           <  2:6719223/93‑1 (MQ=255)

                                               ccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGc                                          >  2:3106511/1‑93 (MQ=255)

                                                 gcgAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAg                                        >  2:1028640/1‑93 (MQ=255)

                                                  cgAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGa                                       >  2:1596585/1‑93 (MQ=255)

                                                  cgAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGa                                       >  1:4711132/1‑93 (MQ=255)

                                                   gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      <  1:289610/93‑1 (MQ=255)

                                                   gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      <  1:5546479/93‑1 (MQ=255)

                                                   gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      <  2:5738581/93‑1 (MQ=255)

                                                    aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATg                                     >  2:3016356/1‑93 (MQ=255)

                                                    aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATg                                     >  1:1703243/1‑93 (MQ=255)

                                                    aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATg                                     >  2:75598/1‑93 (MQ=255)

                                                      aTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGt                                   >  1:5645885/1‑93 (MQ=255)

                                                      aTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGt                                   >  2:6612185/1‑93 (MQ=255)

                                                       ttCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTc                                  <  2:4742995/93‑1 (MQ=255)

                                                       ttCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTc                                  >  1:2324152/1‑93 (MQ=255)

                                                       ttCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTc                                  <  2:3055790/93‑1 (MQ=255)

                                                          gcgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCa                               >  2:1937953/1‑93 (MQ=255)

                                                          gcgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCa                               >  2:2571881/1‑93 (MQ=255)

                                                           cgAAGAACTGGGAGATCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              <  1:6713297/93‑1 (MQ=255)

                                                           cgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              <  1:812914/93‑1 (MQ=255)

                                                           cgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              <  1:1537903/93‑1 (MQ=255)

                                                           cgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              <  1:492100/93‑1 (MQ=255)

                                                            gaagaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAg                             <  1:1040727/93‑1 (MQ=255)

                                                             aagaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAgg                            >  2:1061331/1‑93 (MQ=255)

                                                              agaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGa                           >  2:2039688/1‑93 (MQ=255)

                                                               gaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGaa                          <  2:5550539/93‑1 (MQ=255)

                                                               gaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGaa                          <  2:4350097/93‑1 (MQ=255)

                                                                 aCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAaga                        <  1:423645/93‑1 (MQ=255)

                                                                  cTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAagag                       >  2:3544045/1‑93 (MQ=255)

                                                                   tGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAgaga                      <  1:5444773/93‑1 (MQ=255)

                                                                    gggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAt                     >  1:5705769/1‑93 (MQ=255)

                                                                     ggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGGCCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAtt                    <  1:2969042/93‑1 (MQ=255)

                                                                     ggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAtt                    <  1:4995102/93‑1 (MQ=255)

                                                                     ggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAtt                    >  1:3421386/1‑93 (MQ=255)

                                                                        gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  1:2571718/1‑93 (MQ=255)

                                                                        gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  1:5924963/1‑93 (MQ=255)

                                                                         aaCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                >  1:62993/1‑93 (MQ=255)

                                                                          aCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTatat               <  2:3240417/93‑1 (MQ=255)

                                                                          aCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTatat               >  1:3401901/1‑93 (MQ=255)

                                                                          aCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTatat               >  2:6087393/1‑93 (MQ=255)

                                                                           cAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATg              <  1:2852281/93‑1 (MQ=255)

                                                                           cAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATg              <  2:5054027/93‑1 (MQ=255)

                                                                            aGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGa             >  1:2170113/1‑93 (MQ=255)

                                                                             gctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAt            <  2:815698/93‑1 (MQ=255)

                                                                             gctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAt            <  1:6141174/93‑1 (MQ=255)

                                                                              ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           >  2:62959/1‑93 (MQ=255)

                                                                                 ctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTAc        <  1:729398/93‑1 (MQ=255)

                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      >  2:2248965/1‑93 (MQ=255)

                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAAAGATTTATATGATTTACCt      <  1:6564324/93‑1 (MQ=255)

                                                                                     tGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGa    <  1:4613887/93‑1 (MQ=255)

                                                                                      gACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAt   <  2:6143537/93‑1 (MQ=255)

                                                                                       aCAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAtt  <  1:1359898/93‑1 (MQ=255)

                                                                                       aCAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAtt  <  2:5088258/93‑1 (MQ=255)

TCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT  >  NC_000913/2362679‑2362858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`