BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,387,335	T→C	V68A (GTA→GCA)	b2275 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,387,335	0	T	C	99.6%	816.4 / NA	250	V68A (GTA→GCA)	b2275	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (134/115); total (134/116)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

ACGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGTACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATC  >  NC_000913/2387245‑2387424

aCGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCAc                                                                                         >  1:4835546/1‑93 (MQ=255)

 cGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCAcc                                                                                        <  2:4979570/93‑1 (MQ=255)

  ggAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCt                                                                                       >  2:2564649/1‑93 (MQ=255)

  ggAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCt                                                                                       >  2:3051998/1‑93 (MQ=255)

  ggAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCt                                                                                       >  2:2072094/1‑93 (MQ=255)

    agaCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGc                                                                                     >  1:6612300/1‑93 (MQ=255)

     gaCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGcc                                                                                    <  2:2328261/93‑1 (MQ=255)

     gaCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGcc                                                                                    <  2:1953718/93‑1 (MQ=255)

      aCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCt                                                                                   <  1:5453790/93‑1 (MQ=255)

      aCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCt                                                                                   <  2:620793/93‑1 (MQ=255)

      aCTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCt                                                                                   <  2:1223444/93‑1 (MQ=255)

       cTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCtt                                                                                  >  1:1300927/1‑93 (MQ=255)

       cTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCtt                                                                                  >  2:3115350/1‑93 (MQ=255)

        tGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCttt                                                                                 >  1:2286594/1‑93 (MQ=255)

           ttCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTcc                                                                              <  1:5212728/93‑1 (MQ=255)

               gTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCa                                                                          >  2:5692503/1‑93 (MQ=255)

                tttGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCAt                                                                         >  1:2329903/1‑93 (MQ=255)

                tttGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCAt                                                                         <  2:3787728/93‑1 (MQ=255)

                tttGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCAt                                                                         <  2:6483988/93‑1 (MQ=255)

                tttGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGAACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCAt                                                                         >  1:5800201/1‑93 (MQ=255)

                   gTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaa                                                                      <  1:3258178/93‑1 (MQ=255)

                    tACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaag                                                                     <  1:5514343/93‑1 (MQ=255)

                    tACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACAATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaag                                                                     >  2:4460784/1‑93 (MQ=255)

                     aCTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaaga                                                                    >  1:5591854/1‑93 (MQ=255)

                     aCTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaaga                                                                    >  2:6589620/1‑93 (MQ=255)

                      cTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTaagaa                                                                   >  2:6282693/1‑93 (MQ=255)

                        ggCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGt                                                                 >  1:4348269/1‑93 (MQ=255)

                        ggCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGt                                                                 <  2:388164/93‑1 (MQ=255)

                        ggCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGt                                                                 >  2:4626481/1‑93 (MQ=255)

                         gCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGtt                                                                <  1:1772088/93‑1 (MQ=255)

                             aTTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCgaa                                                            <  2:6377243/93‑1 (MQ=255)

                              ttGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCgaag                                                           <  2:4568961/93‑1 (MQ=255)

                                ggTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCgaagaa                                                         <  1:5669516/93‑1 (MQ=255)

                                ggTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCgaagaa                                                         >  1:3897644/1‑93 (MQ=255)

                                  tCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTTGAAGAATg                                                       <  1:6547346/93‑1 (MQ=255)

                                  tCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATg                                                       <  1:6129093/93‑1 (MQ=255)

                                    aGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGc                                                     <  2:2427949/93‑1 (MQ=255)

                                    aGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGc                                                     >  1:2475061/1‑93 (MQ=255)

                                    aGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGc                                                     <  2:4750353/93‑1 (MQ=255)

                                     ggAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCt                                                    >  2:2758178/1‑93 (MQ=255)

                                     ggAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCt                                                    >  2:3391161/1‑93 (MQ=255)

                                     ggAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCt                                                    >  1:807300/1‑93 (MQ=255)

                                      gAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTg                                                   >  1:1664173/1‑93 (MQ=255)

                                      gAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTg                                                   <  1:1072987/93‑1 (MQ=255)

                                      gAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTg                                                   >  1:3439219/1‑93 (MQ=255)

                                       aGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTgg                                                  >  2:5883513/1‑93 (MQ=255)

                                        gCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGa                                                 >  1:2742233/1‑93 (MQ=255)

                                         cATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGaa                                                <  1:6235102/93‑1 (MQ=255)

                                          aTAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAg                                               <  1:2638696/93‑1 (MQ=255)

                                          aTAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTGTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGGAg                                               >  1:6146023/1‑93 (MQ=255)

                                           tAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAgg                                              >  1:5223186/1‑92 (MQ=255)

                                             aaCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGa                                            >  2:5512184/1‑93 (MQ=255)

                                             aaCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGa                                            >  1:322676/1‑93 (MQ=255)

                                             aaCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGa                                            <  2:4421638/93‑1 (MQ=255)

                                              aCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATTGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAACTGaa                                           >  2:4065247/1‑93 (MQ=255)

                                              aCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGaa                                           <  2:5971830/93‑1 (MQ=255)

                                              aCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGaa                                           >  2:8002/1‑93 (MQ=255)

                                              aCTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGaa                                           <  1:3140996/93‑1 (MQ=255)

                                                tACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatg                                         >  2:6536169/1‑93 (MQ=255)

                                                tACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatg                                         <  1:4938709/93‑1 (MQ=255)

                                                 aCCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatga                                        <  1:4105942/93‑1 (MQ=255)

                                                 aCCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatga                                        >  1:3581754/1‑93 (MQ=255)

                                                  ccTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatgat                                       <  2:1652565/93‑1 (MQ=255)

                                                  ccTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatgat                                       >  2:3238801/1‑93 (MQ=255)

                                                   cTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAatgatg                                      >  1:4172411/1‑93 (MQ=255)

                                                       aGTGTGCGAAACGTACCATGACGAAGGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGGAGTGAAtgaggatga                                  >  1:1351128/1‑93 (MQ=255)

                                                           tgCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTGCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATccc                              >  2:3575648/1‑93 (MQ=255)

                                                            gCGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATcccc                             >  1:5120749/1‑93 (MQ=255)

                                                             cGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCa                            >  2:5088378/1‑93 (MQ=255)

                                                             cGAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCa                            >  1:2884609/1‑93 (MQ=255)

                                                              gAAACGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCAt                           >  2:5394026/1‑93 (MQ=255)

                                                                aaCGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGa                         >  2:4023344/1‑93 (MQ=255)

                                                                aaCGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGa                         <  2:4198122/93‑1 (MQ=255)

                                                                aaCGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGa                         >  1:4467460/1‑93 (MQ=255)

                                                                 aCGTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGaa                        >  2:517727/1‑93 (MQ=255)

                                                                   gTACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGaaaa                      <  1:6430904/93‑1 (MQ=255)

                                                                    tACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAAt                     >  1:5061843/1‑93 (MQ=255)

                                                                    tACCATGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAAt                     <  2:6517984/93‑1 (MQ=255)

                                                                     aCCATGACGAATGGCGAATCGCCCCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATg                    <  1:2025673/93‑1 (MQ=255)

                                                                        aTGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCa                 >  2:2849004/1‑93 (MQ=255)

                                                                         tGACGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCAt                >  1:4258609/1‑93 (MQ=255)

                                                                           aCGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCa              <  2:6704595/93‑1 (MQ=255)

                                                                            cGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAg             >  2:2186345/1‑93 (MQ=255)

                                                                            cGAATGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAg             >  2:4859275/1‑93 (MQ=255)

                                                                              aaTGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCa           >  2:1741544/1‑93 (MQ=255)

                                                                              aaTGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCa           <  2:3702749/93‑1 (MQ=255)

                                                                               aTGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATTGCATCAGCAt          >  1:2589269/1‑93 (MQ=255)

                                                                               aTGGCGAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCAt          >  1:3554305/1‑93 (MQ=255)

                                                                                    gAATCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAg     <  1:1038747/93‑1 (MQ=255)

                                                                                     aaTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGa    >  1:3763592/1‑93 (MQ=255)

                                                                                     aaTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGa    <  1:2156332/93‑1 (MQ=255)

                                                                                     aaTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGa    >  2:1543220/1‑93 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt   <  2:6010422/93‑1 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt   >  2:1045993/1‑93 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt   >  2:4338692/1‑93 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt   <  2:6674031/93‑1 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGAt   >  2:2501795/1‑93 (MQ=255)

                                                                                      aTCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCCAAGAt   >  2:3319982/1‑93 (MQ=255)

                                                                                       tCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATc  >  1:3353508/1‑93 (MQ=255)

                                                                                       tCGCACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATc  <  2:2838918/93‑1 (MQ=255)

ACGGAGACTGTTTCGGTTTGTACTGGCCTATTGGTCAGGAGCATAAACTACCTATAGTGTGCGAAACGTACCATGACGAATGGCGAATCGTACCTGCCTTTTCCAGCATTAAGAAGTTCGAAGAATGGCTGGAAGTGAATGATGATGATCCCCATGAAAATGGCATCAGCATCGAAGATC  >  NC_000913/2387245‑2387424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`