BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,505,495	C→T	Q140Q (CAG→CAA)	b2386 ←	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,505,495	0	C	T	100.0%	476.1 / NA	138	Q140Q (CAG→CAA)	b2386	putative transport protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (106/32); total (106/32)

Only 100 of 164 total aligned reads displayed.

AATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAA  >  NC_000913/2505405‑2505583

aataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTg                                                                                        <  1:263220/93‑1 (MQ=255)

aataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTg                                                                                        <  2:5835697/93‑1 (MQ=255)

 ataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTgg                                                                                       <  1:5517074/93‑1 (MQ=255)

  taatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTggg                                                                                      <  2:2857516/93‑1 (MQ=255)

   aatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGt                                                                                     >  2:3637669/1‑93 (MQ=255)

    atCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTa                                                                                    <  2:1272726/93‑1 (MQ=255)

    atCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTa                                                                                    <  1:277682/93‑1 (MQ=255)

    atCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTa                                                                                    >  2:6606172/1‑93 (MQ=255)

     tCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAg                                                                                   >  2:1944464/1‑93 (MQ=255)

     tCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAg                                                                                   >  2:2998612/1‑93 (MQ=255)

      cAGCGCACCAATGAAGGTGGACGGGACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGa                                                                                  <  1:5784730/93‑1 (MQ=255)

      cAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGa                                                                                  <  1:2514726/93‑1 (MQ=255)

      cAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGa                                                                                  >  2:6635020/1‑93 (MQ=255)

       aGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGAc                                                                                 >  1:14730/1‑93 (MQ=255)

       aGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGAc                                                                                 <  1:414133/93‑1 (MQ=255)

       aGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGGTGGGTAGAc                                                                                 >  1:5547695/1‑93 (MQ=255)

        gcgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACa                                                                                <  2:1932846/93‑1 (MQ=255)

         cgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACAt                                                                               <  1:5648492/93‑1 (MQ=255)

         cgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACAt                                                                               <  1:1161247/93‑1 (MQ=255)

            aCCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCaa                                                                            <  1:5104846/93‑1 (MQ=255)

             ccAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAAc                                                                           <  2:4222436/93‑1 (MQ=255)

             ccAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAAc                                                                           >  1:130685/1‑93 (MQ=255)

              cAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAAcc                                                                          <  2:4654574/93‑1 (MQ=255)

               aaTGAAGGTGGACGGCACCGGCGAAGAGGTCGCCTATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAAccc                                                                         >  2:6243010/1‑93 (MQ=255)

                aTGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACccg                                                                        >  2:829439/1‑93 (MQ=255)

                aTGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACccg                                                                        >  1:3657789/1‑93 (MQ=255)

                   aaGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACccgccg                                                                     >  2:309493/1‑93 (MQ=255)

                   aaGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGGTT‑AGCAGTCGCGTCGGTTGGGTAGACATCAACccgccg                                                                     >  2:3851442/1‑93 (MQ=255)

                    aGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGa                                                                    >  1:5795057/1‑93 (MQ=255)

                     ggTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAt                                                                   >  2:6409736/1‑93 (MQ=255)

                     ggTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAt                                                                   >  2:6234450/1‑93 (MQ=255)

                     ggTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAt                                                                   >  1:4636491/1‑93 (MQ=255)

                     ggTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAt                                                                   >  2:4579185/1‑93 (MQ=255)

                         gACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATaaaa                                                               >  2:2382165/1‑93 (MQ=255)

                         gACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATaaaa                                                               >  1:3388696/1‑93 (MQ=255)

                          aCGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAAc                                                              >  1:4405322/1‑93 (MQ=255)

                           gggCCCCGGCGAAGGGGCCCCCCATTGCATAGTGCTGGGATGGAGGTTA‑GGCGGTGGCGTGGTTTGGGAAGACATCACCCCGCCGATAAAAcc                                                             <  2:265856/92‑1 (MQ=255)

                             gccccGGCGAAGAGGTCGCCCATTGGATGGTGCGGGGCTAGAGGTTC‑AGCAGTTGCGGCGGTTGGGAAGACATCAACCCGCCGATAAAACCAg                                                           <  2:1803867/90‑1 (MQ=255)

                               acgggCGAGGAGGTCGCCCGTTGAATGGTGGTGGAATCAAAGTTC‑AGCAGTGGCGTGGTTGGGGTAGACAACAACCCGCCGATAAAACCAGcc                                                         <  2:2549673/90‑1 (MQ=255)

                                ccGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCg                                                        >  1:3142625/1‑93 (MQ=255)

                                ccGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCg                                                        >  1:5005036/1‑93 (MQ=255)

                                   gCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGa                                                     >  2:2620443/1‑93 (MQ=255)

                                    cGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGaa                                                    >  1:2178196/1‑93 (MQ=255)

                                    cGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGaa                                                    >  2:1015426/1‑93 (MQ=255)

                                    cGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGACGGGaa                                                    >  1:6707100/1‑93 (MQ=255)

                                     gAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAAc                                                   >  2:5379602/1‑93 (MQ=255)

                                     gAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAAc                                                   >  1:4737302/1‑93 (MQ=255)

                                         agGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCa                                               >  2:3161329/1‑93 (MQ=255)

                                         agGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCa                                               >  1:6368163/1‑93 (MQ=255)

                                         agGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCa                                               >  1:512076/1‑93 (MQ=255)

                                           gTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGt                                             >  2:369128/1‑93 (MQ=255)

                                           gTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGt                                             >  1:2312038/1‑93 (MQ=255)

                                           gTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGt                                             >  1:5677406/1‑93 (MQ=255)

                                             cGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGttt                                           >  2:1628378/1‑93 (MQ=255)

                                             cGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGgtt                                           >  2:2756721/1‑93 (MQ=255)

                                              gCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTg                                          >  1:2593528/1‑93 (MQ=255)

                                              gCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTg                                          >  1:1466285/1‑93 (MQ=255)

                                              ccacATTGCATAGTGGTGGTATGAAAGTAC‑AGCTGTTGCTTCGTTTGGGTAGACAACACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTg                                          <  1:738946/90‑1 (MQ=255)

                                               cccATTGCATAGTGCGGGGATGAAAGTTC‑AGCATTGGCGTCGTTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGc                                         <  2:5896669/93‑1 (MQ=255)

                                                ccATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                        >  2:1307888/1‑93 (MQ=255)

                                                ccATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                        >  1:1643845/1‑93 (MQ=255)

                                                ccATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                        >  1:2248576/1‑93 (MQ=255)

                                                  aTTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACGCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAc                                      >  1:2369518/1‑93 (MQ=255)

                                                  aTTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAc                                      >  1:1763697/1‑93 (MQ=255)

                                                   ttGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc                                     >  1:4362505/1‑93 (MQ=255)

                                                   ttGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc                                     >  2:710212/1‑93 (MQ=255)

                                                   tcgaaTAGTGGTGGGACCGAAGTTC‑AGCATTGGCGGGGTTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc                                     <  2:1225266/89‑1 (MQ=255)

                                                     gCATAGGGCGGGGATCAAAGTGC‑AGCGGTTGCGTGGGTGGGGTAAACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGa                                   <  1:2930213/93‑1 (MQ=255)

                                                     gCAGAGTGGTGGGA‑CGTAGTTCAAGCAGTTGGGTCGGTGGGGTAACAACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGa                                   <  1:5209142/93‑1 (MQ=255)

                                                       aTAGTGTTGGGATGGAATTTC‑AGCAGTGGCGTGGTTGGGGTAAACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATa                                 <  1:5488732/93‑1 (MQ=255)

                                                       aTAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATa                                 >  2:699405/1‑93 (MQ=255)

                                                       aTAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATa                                 >  1:6482067/1‑93 (MQ=255)

                                                       aTAGTGCGGGGATCGAGGTGA‑AGCAGTGGCGTGGGTGGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATa                                 <  2:614342/93‑1 (MQ=255)

                                                          gTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATagag                              >  2:5615235/1‑93 (MQ=255)

                                                          gTGCTGAGATCAAAGTTC‑GGGATGTGGGTGGGTTGGGTAAACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATagag                              <  2:3966443/93‑1 (MQ=255)

                                                          gTGCGGGGATCGAAGTTC‑AGCAGTGGCGTCGGTTGGGTAAACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATagag                              <  1:2149430/93‑1 (MQ=255)

                                                           tGCGGGGATCGAATTTC‑AGCAGTGGCGTGGGTGGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGt                             <  2:4802897/93‑1 (MQ=255)

                                                            gCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGtt                            >  2:418264/1‑93 (MQ=255)

                                                            gCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGtt                            >  1:992604/1‑93 (MQ=255)

                                                              tGGGATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCg                          >  1:3045179/1‑93 (MQ=255)

                                                               gggATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGc                         >  2:4955822/1‑93 (MQ=255)

                                                               gggATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGGTCGc                         >  1:474479/1‑93 (MQ=255)

                                                               gggATCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGATCGc                         >  1:4163017/1‑93 (MQ=255)

                                                                   tCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCcaca                     >  1:6264599/1‑93 (MQ=255)

                                                                   tCGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCcaca                     >  2:653875/1‑93 (MQ=255)

                                                                    cGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaa                    >  1:6139074/1‑93 (MQ=255)

                                                                    cGAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaa                    >  1:3324118/1‑93 (MQ=255)

                                                                     gAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaa                   >  2:5727661/1‑93 (MQ=255)

                                                                     gAAGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaa                   >  1:1747615/1‑93 (MQ=255)

                                                                      aatttC‑CGCGGTTGCGTGGGTGGGGAAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa                  <  1:2169423/90‑1 (MQ=255)

                                                                      aaGTTC‑AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa                  >  2:5133692/1‑93 (MQ=255)

                                                                             agcagTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAgcagca            >  1:795624/1‑93 (MQ=255)

                                                                              gcagTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaa           >  1:4286175/1‑93 (MQ=255)

                                                                               cagTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaaa          >  2:434701/1‑93 (MQ=255)

                                                                               cagTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaaa          >  2:1399604/1‑93 (MQ=255)

                                                                               cagTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaaa          >  2:3453531/1‑93 (MQ=255)

                                                                                   tGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATc      >  2:306768/1‑93 (MQ=255)

                                                                                   tGCGTCGGTCGGGTACACAACACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATc      <  2:4139215/93‑1 (MQ=255)

                                                                                     cGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCgg    >  2:5937618/1‑93 (MQ=255)

                                                                                       tCGGTTGGGTAGACATCAACCAGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGaa  >  1:4242664/1‑93 (MQ=255)

AATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTC‑AGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAA  >  NC_000913/2505405‑2505583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`