BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,515,312	T→C	M181V (ATG→GTG)	b2395 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,515,312	0	T	C	99.5%	686.5 / NA	210	M181V (ATG→GTG)	b2395	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (98/111); minor base G (0/1); total (98/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTA  >  NC_000913/2515222‑2515401

ctTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCAcgt                                                                                         >  2:5560621/1‑93 (MQ=255)

ctTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCAcgt                                                                                         <  1:740394/93‑1 (MQ=255)

 tttGCCCAACGACGGAGCGCTATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGCGAGATAGTAGAAAAGCAcgtt                                                                                        >  2:3457858/1‑93 (MQ=255)

 tttGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCAcgtt                                                                                        >  2:6667165/1‑93 (MQ=255)

 tttGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCAcgtt                                                                                        >  2:434919/1‑93 (MQ=255)

 tttGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCAcgtt                                                                                        >  2:4588711/1‑93 (MQ=255)

   tGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCAcgttgt                                                                                      <  2:3916072/93‑1 (MQ=255)

   tGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCAcgttgt                                                                                      <  2:1019009/93‑1 (MQ=255)

   tGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCAcgttgt                                                                                      >  1:1629162/1‑93 (MQ=255)

   tGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCAcgttgt                                                                                      >  1:452414/1‑93 (MQ=255)

      ccAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTaaa                                                                                   >  2:159336/1‑93 (MQ=255)

      ccAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTaaa                                                                                   >  2:1522180/1‑93 (MQ=255)

      ccAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTaaa                                                                                   <  2:3748502/93‑1 (MQ=255)

         acgacgGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCa                                                                                <  2:2050569/93‑1 (MQ=255)

         acgacgGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCa                                                                                >  1:1329972/1‑93 (MQ=255)

            acgGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGca                                                                             >  1:6396038/1‑93 (MQ=255)

            acgGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGca                                                                             >  1:6031373/1‑93 (MQ=255)

             cgGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGcac                                                                            <  1:3285265/93‑1 (MQ=255)

               gAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAg                                                                          <  1:3482086/93‑1 (MQ=255)

                aGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGc                                                                         >  1:4867246/1‑93 (MQ=255)

                aGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGc                                                                         <  2:5425470/93‑1 (MQ=255)

                 gCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCg                                                                        >  1:2977408/1‑93 (MQ=255)

                  cGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCgg                                                                       <  2:4659450/93‑1 (MQ=255)

                  cGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCgg                                                                       >  2:413544/1‑93 (MQ=255)

                   gATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGt                                                                      >  1:2622799/1‑93 (MQ=255)

                   gATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGt                                                                      <  1:5945176/93‑1 (MQ=255)

                   gATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGt                                                                      >  1:1449998/1‑93 (MQ=255)

                    atatCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTg                                                                     >  1:1427104/1‑93 (MQ=255)

                    atatCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGATGTAAATCAGCACAGCGGta                                                                     >  1:6092498/1‑92 (MQ=255)

                      atCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGaa                                                                   >  1:4174487/1‑93 (MQ=255)

                      atCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGaa                                                                   >  1:5722580/1‑93 (MQ=255)

                      atCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGaa                                                                   >  1:6229220/1‑93 (MQ=255)

                        cccTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGaaaa                                                                 <  1:5360660/93‑1 (MQ=255)

                        cccTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGaaaa                                                                 >  2:5980448/1‑93 (MQ=255)

                           tGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGc                                                              >  1:4561678/1‑93 (MQ=255)

                           tGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGc                                                              >  1:2014277/1‑93 (MQ=255)

                             cgcCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCtt                                                            <  1:4239886/93‑1 (MQ=255)

                              gcCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTa                                                           <  2:3692037/93‑1 (MQ=255)

                              gcCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTa                                                           <  1:1533309/93‑1 (MQ=255)

                              gcCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTa                                                           >  2:5659165/1‑93 (MQ=255)

                              gcCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGATTa                                                           >  1:386528/1‑93 (MQ=255)

                                cacaATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGc                                                         <  1:3588048/93‑1 (MQ=255)

                                 acaATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCa                                                        >  2:4343365/1‑93 (MQ=255)

                                   aaTATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCaaa                                                      >  2:3284827/1‑93 (MQ=255)

                                   aaTATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCaaa                                                      >  1:1891404/1‑93 (MQ=255)

                                   aaTATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCaaa                                                      <  2:1034002/93‑1 (MQ=255)

                                   aaTATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCaaa                                                      >  2:1404558/1‑93 (MQ=255)

                                    atatCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAAt                                                     >  2:808558/1‑93 (MQ=255)

                                    atatCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAAt                                                     >  1:3321742/1‑93 (MQ=255)

                                    atatCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAAt                                                     <  2:6252770/93‑1 (MQ=255)

                                     tatCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAACGCAGGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATc                                                    <  1:5521293/93‑1 (MQ=255)

                                       tCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCaa                                                  <  1:5019191/93‑1 (MQ=255)

                                         tGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACTTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACg                                                <  2:3567656/93‑1 (MQ=255)

                                            gcAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGAcc                                             <  2:4184889/93‑1 (MQ=255)

                                              aaaGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGAGGTGAAAAGGCTTAGCAAATCAACGACAGt                                           >  1:2539306/1‑93 (MQ=255)

                                               aaGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTg                                          >  1:461464/1‑93 (MQ=255)

                                               aaGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTg                                          >  1:1529240/1‑93 (MQ=255)

                                                aGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGa                                         <  1:245783/93‑1 (MQ=255)

                                                aGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGAAAATCAACGACCGTGa                                         >  2:2106647/1‑93 (MQ=255)

                                                 gTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGaa                                        <  2:1838036/93‑1 (MQ=255)

                                                   ggggACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGaaaa                                      >  2:3343157/1‑93 (MQ=255)

                                                   ggggACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGaaaa                                      <  1:646733/93‑1 (MQ=255)

                                                   ggggACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGaaaa                                      >  1:2591686/1‑93 (MQ=255)

                                                    gggACTTACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCCCAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAAt                                     >  2:2855824/1‑93 (MQ=255)

                                                          tACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATc                               <  1:4369516/93‑1 (MQ=255)

                                                          tACAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATc                               <  2:5740066/93‑1 (MQ=255)

                                                           aCAATCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATcc                              <  2:1826686/93‑1 (MQ=255)

                                                              aTCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCa                           >  2:1356152/1‑93 (MQ=255)

                                                              aTCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCa                           >  2:1042877/1‑93 (MQ=255)

                                                              aTCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCa                           <  1:2047910/93‑1 (MQ=255)

                                                               tCATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCAt                          <  1:4825605/93‑1 (MQ=255)

                                                                cATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATc                         <  1:4442530/93‑1 (MQ=255)

                                                                cATGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATc                         <  2:5114820/93‑1 (MQ=255)

                                                                  tGCGAGTGNGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGc                       <  1:4380916/93‑1 (MQ=255)

                                                                  tGCGAGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGc                       >  1:1168820/1‑93 (MQ=255)

                                                                      aGTGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaa                   >  2:2299517/1‑93 (MQ=255)

                                                                        tGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaaaa                 >  2:3017152/1‑93 (MQ=255)

                                                                        tGAGATAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaaaa                 >  2:2715315/1‑93 (MQ=255)

                                                                         gagaTAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaaaaa                <  1:396631/93‑1 (MQ=255)

                                                                         gagaTAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaaaaa                <  2:6343657/93‑1 (MQ=255)

                                                                         gagaTAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaaaaa                <  1:4105512/93‑1 (MQ=255)

                                                                         gagaTAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGaaaaa                <  1:1165156/93‑1 (MQ=255)

                                                                          agaTAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAg               <  2:2439819/93‑1 (MQ=255)

                                                                           gaTAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAgg              <  1:4757326/93‑1 (MQ=255)

                                                                            aTAGTAGAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGt             <  2:3968552/93‑1 (MQ=255)

                                                                             tagtagAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTg            >  2:3253207/1‑93 (MQ=255)

                                                                             tagtagAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTg            >  2:2782661/1‑93 (MQ=255)

                                                                              agtagAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTgg           <  1:1019785/93‑1 (MQ=255)

                                                                              agtagAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTgg           <  1:3789467/93‑1 (MQ=255)

                                                                               gtagAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGa          >  1:4035344/1‑93 (MQ=255)

                                                                               gtagAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGa          <  1:3155587/93‑1 (MQ=255)

                                                                               gtagAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGa          <  1:2864955/93‑1 (MQ=255)

                                                                               gtagAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGa          <  2:6610531/93‑1 (MQ=255)

                                                                               gtagAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGa          <  2:2578315/93‑1 (MQ=255)

                                                                                 agAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGata        <  2:1940431/93‑1 (MQ=255)

                                                                                 aaaaaaGCCCGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGata        <  1:120896/91‑1 (MQ=255)

                                                                                  gAAAAGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGatat       >  1:985526/1‑93 (MQ=255)

                                                                                   aaaaGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATc      <  2:3624831/93‑1 (MQ=255)

                                                                                    aaaGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCt     >  1:1565917/1‑93 (MQ=255)

                                                                                    aaaGCACGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCt     <  1:921935/93‑1 (MQ=255)

CTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTA  >  NC_000913/2515222‑2515401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`