BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,532,993	A→G	D303G (GAC→GGC)	ptsI →	PEP‑protein phosphotransferase system enzyme I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,532,993	0	A	G	100.0%	890.3 / NA	275	D303G (GAC→GGC)	ptsI	PEP‑protein phosphotransferase system enzyme I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (118/157); total (118/157)

Only 100 of 278 total aligned reads displayed.

CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA  >  NC_000913/2532903‑2533082

cTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGcc                                                                                         >  2:4315211/1‑93 (MQ=255)

 tAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcg                                                                                        >  1:811856/1‑93 (MQ=255)

   aCATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcgcg                                                                                      >  2:5023413/1‑93 (MQ=255)

   aCATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcgcg                                                                                      >  2:55554/1‑93 (MQ=255)

    cATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGa                                                                                     <  1:3889407/93‑1 (MQ=255)

     aTTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAc                                                                                    >  2:4645243/1‑93 (MQ=255)

     aTTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAc                                                                                    <  2:5622105/93‑1 (MQ=255)

     aTTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAc                                                                                    <  2:1765315/93‑1 (MQ=255)

      ttGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACg                                                                                   <  2:2194541/93‑1 (MQ=255)

         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  1:5231668/93‑1 (MQ=255)

         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  2:4920291/93‑1 (MQ=255)

          tACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACt                                                                               <  1:4612103/93‑1 (MQ=255)

          tACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACt                                                                               <  1:3541036/93‑1 (MQ=255)

           aCGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTg                                                                              >  2:5168628/1‑93 (MQ=255)

              gTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGccc                                                                           >  2:1700461/1‑93 (MQ=255)

                 cGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACt                                                                        >  2:3369490/1‑93 (MQ=255)

                 cGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACt                                                                        >  1:2287857/1‑93 (MQ=255)

                  gTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTg                                                                       <  2:4251318/93‑1 (MQ=255)

                  gTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGGATCGTACTGAGTTCCTGTTCATGGGCCGCTACGCACTGCCCACTg                                                                       >  2:1153000/1‑93 (MQ=255)

                   tGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTga                                                                      >  1:2566457/1‑93 (MQ=255)

                   tGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTga                                                                      <  2:205483/93‑1 (MQ=255)

                    gACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaa                                                                     <  1:6393497/93‑1 (MQ=255)

                      cGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaaga                                                                   <  1:5343950/93‑1 (MQ=255)

                       gTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaa                                                                  <  2:101595/93‑1 (MQ=255)

                        ttGAAGGTGCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaag                                                                 <  1:5972179/93‑1 (MQ=255)

                         tGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaaga                                                                <  1:315162/93‑1 (MQ=255)

                         tGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaaga                                                                <  1:3328838/93‑1 (MQ=255)

                          gAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaagaa                                                               <  2:375679/93‑1 (MQ=255)

                          gAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTAGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCCCTGCCCACTgaagaagaa                                                               <  2:3899316/93‑1 (MQ=255)

                           aaGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAc                                                              >  1:4146767/1‑93 (MQ=255)

                            aGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACa                                                             <  1:4395492/93‑1 (MQ=255)

                            aGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACa                                                             >  1:4706178/1‑93 (MQ=255)

                            aGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACa                                                             >  1:5203097/1‑93 (MQ=255)

                             ggTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAg                                                            >  2:3500523/1‑93 (MQ=255)

                             ggTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAg                                                            <  1:3101392/93‑1 (MQ=255)

                              gTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGt                                                           <  1:5532425/93‑1 (MQ=255)

                               tGCAGAGCGTAGCGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGtt                                                          <  1:681750/93‑1 (MQ=255)

                               tGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGtt                                                          <  2:3618595/93‑1 (MQ=255)

                                gCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTGCTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGttt                                                         <  1:4958760/93‑1 (MQ=255)

                                gCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGttt                                                         <  1:4065440/93‑1 (MQ=255)

                                gCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGttt                                                         >  1:1596462/1‑93 (MQ=255)

                                gCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGttt                                                         >  1:292392/1‑93 (MQ=255)

                                 cAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtg                                                        <  1:3992224/93‑1 (MQ=255)

                                  agagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgc                                                       <  1:360888/93‑1 (MQ=255)

                                       gTAACGGCACTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAGGAAGAACAGTTTGCTGCtt                                                  <  1:6566017/93‑1 (MQ=255)

                                        taccGGCGCTGAAGGTGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTa                                                 <  1:1413621/90‑1 (MQ=255)

                                        tAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTa                                                 >  2:4521131/1‑93 (MQ=255)

                                        tAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTa                                                 >  1:5451590/1‑93 (MQ=255)

                                          aCGGTGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACa                                               <  2:5829969/93‑1 (MQ=255)

                                          aCGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACa                                               >  1:1048412/1‑93 (MQ=255)

                                          aCGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACa                                               >  2:6013750/1‑93 (MQ=255)

                                          aCGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCACGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACa                                               >  2:2506335/1‑93 (MQ=255)

                                             gcgcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAg                                            >  1:4381375/1‑93 (MQ=255)

                                                cTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAg                                         <  2:4211479/93‑1 (MQ=255)

                                                cTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAg                                         >  2:4514148/1‑93 (MQ=255)

                                                 tGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTATAAAGCAGt                                        >  2:5292818/1‑93 (MQ=255)

                                                   aaGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTgg                                      <  1:4573266/93‑1 (MQ=255)

                                                    aGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGc                                     <  1:643068/93‑1 (MQ=255)

                                                    aGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGc                                     <  2:4591462/93‑1 (MQ=255)

                                                     ggtgTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCt                                    >  1:2653947/4‑93 (MQ=255)

                                                     ggCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCt                                    >  2:958318/1‑93 (MQ=255)

                                                     ggCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCt                                    >  2:3199703/1‑93 (MQ=255)

                                                     ggCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCt                                    <  1:1651568/93‑1 (MQ=255)

                                                       cGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGa                                  <  1:1119889/93‑1 (MQ=255)

                                                        gTTGGTCTGTGTCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGaa                                 <  2:3071906/93‑1 (MQ=255)

                                                        gTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGaa                                 <  2:3006764/93‑1 (MQ=255)

                                                        gTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGaa                                 <  2:4838426/93‑1 (MQ=255)

                                                         ttGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAg                                >  2:2554075/1‑93 (MQ=255)

                                                           ggTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCg                              <  1:1020752/93‑1 (MQ=255)

                                                             tCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtg                            <  2:6548667/93‑1 (MQ=255)

                                                              cTGTATCGTACTGAGTTACTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtgt                           >  2:853112/1‑93 (MQ=255)

                                                               tGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGtgtg                          >  1:4979075/1‑93 (MQ=255)

                                                               tGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGtgtg                          <  1:453396/93‑1 (MQ=255)

                                                               tGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGtgtg                          <  1:4278666/93‑1 (MQ=255)

                                                                  aTCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCt                       <  2:2439370/93‑1 (MQ=255)

                                                                  aTCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCt                       >  1:1251403/1‑93 (MQ=255)

                                                                    cGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCg                     >  1:5660799/1‑93 (MQ=255)

                                                                       aCTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCaa                  >  2:1462803/1‑93 (MQ=255)

                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 <  2:6671458/93‑1 (MQ=255)

                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 <  2:1961823/93‑1 (MQ=255)

                                                                         tGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGc                <  2:1611860/93‑1 (MQ=255)

                                                                         tGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGc                >  1:2120954/1‑93 (MQ=255)

                                                                         tGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGc                <  1:4807200/93‑1 (MQ=255)

                                                                          gAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCg               <  2:5499957/93‑1 (MQ=255)

                                                                          gAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCg               <  2:4186717/93‑1 (MQ=255)

                                                                          gAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCg               <  2:5978248/93‑1 (MQ=255)

                                                                           aGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCgg              >  2:661979/1‑93 (MQ=255)

                                                                             ttCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGtt            <  1:4002507/93‑1 (MQ=255)

                                                                              tCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTa           <  1:1375559/93‑1 (MQ=255)

                                                                              tCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTa           <  1:6113507/93‑1 (MQ=255)

                                                                               ccTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAt          <  1:3023047/93‑1 (MQ=255)

                                                                               ccTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAt          <  2:6117793/93‑1 (MQ=255)

                                                                                cTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtc         >  1:3442328/1‑93 (MQ=255)

                                                                                 tGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcg        >  2:2893808/1‑93 (MQ=255)

                                                                                 tGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGGTAtcg        >  1:2251560/1‑93 (MQ=255)

                                                                                   ttCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgtt      >  2:761443/1‑93 (MQ=255)

                                                                                    tCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttc     <  2:6310072/93‑1 (MQ=255)

                                                                                    tCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttc     <  1:956614/93‑1 (MQ=255)

                                                                                     cATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttcg    <  2:4144091/93‑1 (MQ=255)

                                                                                      aTGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttcgt   <  1:5155562/93‑1 (MQ=255)

CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA  >  NC_000913/2532903‑2533082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`