BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	27,903	T→C	L204P (CTG→CCG)	yaaF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	27,903	0	T	C	99.6%	878.8 / NA	267	L204P (CTG→CCG)	yaaF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (151/115); minor base G (1/0); total (152/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

CCTGTGTCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACTGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACG  >  NC_000913/27813‑27992

ccTGTGTCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGc                                                                                         <  2:3748549/93‑1 (MQ=255)

ccTGTGTCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGc                                                                                         <  2:5549038/93‑1 (MQ=255)

 cTGTGTCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGcc                                                                                        <  1:5006792/93‑1 (MQ=255)

  tgtgTCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACAccgccg                                                                                       >  1:304522/1‑93 (MQ=255)

  tgtgTCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACAccgccg                                                                                       >  1:5771257/1‑93 (MQ=255)

  tgtgTCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGCTTGGATGTCACCAATCAGGCAATATTAACTCTTGACTATCTCTCTACAccgccg                                                                                       >  2:5196696/1‑93 (MQ=255)

    tgtCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCa                                                                                     <  2:6029858/93‑1 (MQ=255)

     gtCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAg                                                                                    >  2:6134816/1‑93 (MQ=255)

      tCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGt                                                                                   <  1:5696458/93‑1 (MQ=255)

          ccGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTaaa                                                                               >  1:1164191/1‑93 (MQ=255)

           cGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAAc                                                                              >  2:3986482/1‑93 (MQ=255)

           cGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAAc                                                                              >  1:2760966/1‑93 (MQ=255)

           cGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAAc                                                                              >  1:2321331/1‑93 (MQ=255)

            gCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAAcc                                                                             >  1:5860453/1‑93 (MQ=255)

             cAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCg                                                                            <  1:2628874/93‑1 (MQ=255)

              aGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGt                                                                           <  1:5575527/93‑1 (MQ=255)

               gTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTa                                                                          >  1:3399066/1‑93 (MQ=255)

               gTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTa                                                                          <  1:2688732/93‑1 (MQ=255)

                tGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTAc                                                                         <  1:3818077/93‑1 (MQ=255)

                 ggTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTAcc                                                                        >  2:5832650/1‑93 (MQ=255)

                 ggTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTAcc                                                                        >  1:952037/1‑93 (MQ=255)

                  gTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCg                                                                       >  1:1516542/1‑93 (MQ=255)

                  gTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCg                                                                       <  2:4322227/93‑1 (MQ=255)

                  gTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCg                                                                       >  2:3471360/1‑93 (MQ=255)

                   tATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCgg                                                                      <  1:4103498/93‑1 (MQ=255)

                    aTTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCggg                                                                     <  2:781807/93‑1 (MQ=255)

                    aTTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCggg                                                                     <  2:17862/93‑1 (MQ=255)

                    aTTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCggg                                                                     <  1:2347538/93‑1 (MQ=255)

                     ttGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGa                                                                    >  2:3207249/1‑93 (MQ=255)

                      tGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGaa                                                                   <  1:3775848/93‑1 (MQ=255)

                         aaTCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAAt                                                                <  2:3911596/93‑1 (MQ=255)

                         aaTCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAAt                                                                <  1:4029518/93‑1 (MQ=255)

                           tCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGc                                                              >  1:5191467/1‑93 (MQ=255)

                           tCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGc                                                              <  1:681977/93‑1 (MQ=255)

                           tCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGc                                                              <  1:6559636/93‑1 (MQ=255)

                             gTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTATCGGGAAAATGCtt                                                            >  1:1625710/1‑93 (MQ=255)

                              tCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTc                                                           <  1:5211846/93‑1 (MQ=255)

                              tCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTc                                                           <  2:5607254/93‑1 (MQ=255)

                                aTGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCAc                                                         >  1:3188763/1‑93 (MQ=255)

                                 tgtgCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACg                                                        >  1:4433960/1‑93 (MQ=255)

                                    gCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACTCCGCCGCAGTTAACCCGTACCGGGAAAATGCTTCACGccc                                                     <  1:1293153/93‑1 (MQ=255)

                                     cGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCt                                                    <  2:952037/93‑1 (MQ=255)

                                     cGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCt                                                    <  2:4354890/93‑1 (MQ=255)

                                      ggTTTGGAGGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTg                                                   <  2:3041268/93‑1 (MQ=255)

                                        tttGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGtt                                                 <  2:2990223/93‑1 (MQ=255)

                                         ttGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGttt                                                <  2:6688530/93‑1 (MQ=255)

                                          tGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTa                                               <  2:6545325/93‑1 (MQ=255)

                                          tGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTa                                               <  2:645903/93‑1 (MQ=255)

                                           ggATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAg                                              >  2:4115293/1‑93 (MQ=255)

                                            gATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGc                                             >  2:3312582/1‑93 (MQ=255)

                                             aTGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGcc                                            >  2:3617420/1‑93 (MQ=255)

                                                  aCCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTAc                                       >  2:5104404/1‑93 (MQ=255)

                                                  aCCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTAc                                       >  1:2990398/1‑93 (MQ=255)

                                                   ccAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTAcc                                      <  2:454873/93‑1 (MQ=255)

                                                   ccAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTAcc                                      <  2:2441501/93‑1 (MQ=255)

                                                   ccAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTAcc                                      >  2:4046401/1‑93 (MQ=255)

                                                    cAATCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCg                                     >  1:1110929/1‑93 (MQ=255)

                                                      aTCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTa                                   >  2:2041119/1‑93 (MQ=255)

                                                      aTCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTa                                   >  2:6358937/1‑93 (MQ=255)

                                                      aTCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTa                                   <  1:1418772/93‑1 (MQ=255)

                                                       tCAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAg                                  >  1:5899556/1‑93 (MQ=255)

                                                        cAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGc                                 >  1:6205487/1‑93 (MQ=255)

                                                        cAGGCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGc                                 <  1:1743643/93‑1 (MQ=255)

                                                          ggCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCgg                               >  2:1476365/1‑93 (MQ=255)

                                                           gCAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGc                              <  2:2671316/93‑1 (MQ=255)

                                                            cAATATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCa                             <  2:799513/93‑1 (MQ=255)

                                                             aaTATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAg                            >  1:2165730/1‑93 (MQ=255)

                                                             aaTATTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAg                            <  2:6589187/93‑1 (MQ=255)

                                                              atatTACCTCCTGCCTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGt                           <  1:3485615/93‑1 (MQ=255)

                                                              atatTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGt                           >  1:6336939/1‑93 (MQ=255)

                                                               tatTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTa                          >  2:4599306/1‑93 (MQ=255)

                                                               tatTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTa                          <  2:6300791/93‑1 (MQ=255)

                                                                atTAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTAt                         <  1:4271538/93‑1 (MQ=255)

                                                                 ttAACTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCACCTACCGAACGGGCAGtgtg                        >  2:1279501/1‑93 (MQ=255)

                                                                   aaCTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCa                      >  2:1924347/1‑93 (MQ=255)

                                                                    aCTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCaa                     >  2:2180623/1‑93 (MQ=255)

                                                                    aCTCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCaa                     >  1:5950816/1‑93 (MQ=255)

                                                                      tCCTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAg                   >  2:5393301/1‑93 (MQ=255)

                                                                       ccTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGc                  >  2:4988551/1‑93 (MQ=255)

                                                                       ccTGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGc                  >  2:3675427/1‑93 (MQ=255)

                                                                         tGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCgg                >  2:2547565/1‑93 (MQ=255)

                                                                         tGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCgg                >  2:4033308/1‑93 (MQ=255)

                                                                         tGACTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCgg                >  2:1698783/1‑93 (MQ=255)

                                                                           aCTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCt              <  2:5975140/93‑1 (MQ=255)

                                                                           aCTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCt              <  1:3282408/93‑1 (MQ=255)

                                                                           aCTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCt              <  1:6156883/93‑1 (MQ=255)

                                                                            cTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCtt             >  1:5461796/1‑93 (MQ=255)

                                                                            cTATCTCTCTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCtt             >  2:3808771/1‑93 (MQ=255)

                                                                               tctctcTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTgag          >  1:1888426/1‑91 (MQ=255)

                                                                               tctctcTACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTTCg          >  1:5314525/1‑93 (MQ=255)

                                                                                 tctctACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTTCGaa        >  2:3177571/1‑93 (MQ=255)

                                                                                 tctctACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTTCGaa        >  1:4833941/1‑93 (MQ=255)

                                                                                 tctctACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGaa        <  2:5789654/93‑1 (MQ=255)

                                                                                 tctctACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGaa        <  2:2834338/93‑1 (MQ=255)

                                                                                   tctACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTTGGAATg      >  2:1372856/1‑93 (MQ=255)

                                                                                   tctACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTTCGAATg      >  1:5117291/1‑93 (MQ=255)

                                                                                   tctACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGGATGCAAAGCGGCTTTAGAATg      >  1:601372/1‑93 (MQ=255)

                                                                                    ctACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGGATGc     >  1:4749754/1‑93 (MQ=255)

                                                                                    ctACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGc     <  1:369033/93‑1 (MQ=255)

                                                                                     tACACCGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCa    <  1:3862270/93‑1 (MQ=255)

CCTGTGTCTTCCGCAGTGGTATTGAAATCGTCATGTGCGGTTTGGATGTCACCAATCAGGCAATATTAACTCCTGACTATCTCTCTACACTGCCGCAGTTAAACCGTACCGGGAAAATGCTTCACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACG  >  NC_000913/27813‑27992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`