BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,668,370	T→C	V440A (GTG→GCG)	hcaA1 →	large terminal subunit of phenylpropionate dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,668,370	0	T	C	100.0%	707.9 / NA	221	V440A (GTG→GCG)	hcaA1	large terminal subunit of phenylpropionate dioxygenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (123/98); total (123/98)

Only 100 of 230 total aligned reads displayed.

GCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGTGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGTTTCT  >  NC_000913/2668280‑2668459

gCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAgcgc                                                                                         <  1:3951492/93‑1 (MQ=255)

gCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAgcgc                                                                                         <  2:6584555/93‑1 (MQ=255)

gCATTACTAACTATAGCTTTTCAGAAACTGCCGCTCGTGGAATGTGCCAACGCTGGGCCGATCTTCGGAGTAGCGAAAGCTGGCAGGAAgcgc                                                                                         <  1:6591589/93‑1 (MQ=255)

 cATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCt                                                                                        <  1:2832761/93‑1 (MQ=255)

   ttACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTAGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCg                                                                                      >  2:3622102/1‑93 (MQ=255)

     actaactaTATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGAt                                                                                    >  2:5969903/1‑93 (MQ=255)

      ctaactaTATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATa                                                                                   >  1:1648797/1‑93 (MQ=255)

      ctaactaTATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATa                                                                                   >  1:1826475/1‑93 (MQ=255)

       taactaTATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATaa                                                                                  >  2:2129309/1‑93 (MQ=255)

       taactaTATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATaa                                                                                  >  1:21664/1‑93 (MQ=255)

        aactaTATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATaaa                                                                                 >  2:163577/1‑93 (MQ=255)

          ctaTATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAAc                                                                               <  1:4718719/93‑1 (MQ=255)

           tataTCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAAcc                                                                              >  1:4854430/1‑93 (MQ=255)

           tataTCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAAcc                                                                              >  1:5013771/1‑93 (MQ=255)

           tataTCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAAcc                                                                              <  2:6220738/93‑1 (MQ=255)

            atatCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAAccg                                                                             >  1:3883986/1‑93 (MQ=255)

             tatCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAAccgc                                                                            >  1:1939426/1‑93 (MQ=255)

               tCTTTTCAGAAACTGCCTCTCGTGGCATGTACCAACGCTGAGCCGATCTTCTTAGTAGCGAAAGCTGGCCGGAACAGCTCGATAAAAcccccg                                                                          >  1:2015237/1‑93 (MQ=255)

               tCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAAccgccg                                                                          >  2:2139510/1‑93 (MQ=255)

                   tgcAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTACCGAAACCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTa                                                                      <  2:6475941/91‑1 (MQ=255)

                    tCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTAc                                                                     >  2:4034962/1‑93 (MQ=255)

                      aGAAACTGCCGCTCATCGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACca                                                                   <  1:5218979/93‑1 (MQ=255)

                       gAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACcag                                                                  >  1:4484929/1‑93 (MQ=255)

                        aaaCTGCCGCACGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCACTCGATAAAACCGCCGCTTACcagc                                                                 >  1:6168965/1‑93 (MQ=255)

                         aaCTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTTGATAAAACCGCCGCTTACcagca                                                                >  1:5290314/1‑93 (MQ=255)

                         aaCTGCCGCTCGTGCAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACcagca                                                                <  1:429360/93‑1 (MQ=255)

                          aCTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACcagcag                                                               <  1:2594937/93‑1 (MQ=255)

                            tGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCagga                                                             >  1:4338326/1‑93 (MQ=255)

                            tGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCagga                                                             >  2:3818704/1‑93 (MQ=255)

                            tGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCagga                                                             <  2:4556236/93‑1 (MQ=255)

                             gCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCaggag                                                            <  1:1142946/93‑1 (MQ=255)

                             gCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCaggag                                                            <  2:1106420/93‑1 (MQ=255)

                             gCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCaggag                                                            <  1:6729475/93‑1 (MQ=255)

                             gCCGCTCGTGGAACGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCaggag                                                            >  1:149300/1‑93 (MQ=255)

                             gCCGCTCGTGAAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCGCGATAAAACCGCCGCTTACCAGCaggag                                                            <  1:6101690/93‑1 (MQ=255)

                              ccGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCaggagg                                                           <  1:4164261/93‑1 (MQ=255)

                              ccGCTCGTGGAATGTACCAACGCTGGGCAGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCaggagg                                                           <  1:173070/93‑1 (MQ=255)

                               cGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGt                                                          <  2:5313171/93‑1 (MQ=255)

                               cGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGt                                                          <  2:2182940/93‑1 (MQ=255)

                                gCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCaggagggg                                                         >  2:1400733/1‑91 (MQ=255)

                                gCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGtg                                                         <  1:2876070/93‑1 (MQ=255)

                                 cTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGtga                                                        <  1:2355070/93‑1 (MQ=255)

                                 cTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGtga                                                        <  1:5948651/93‑1 (MQ=255)

                                  tCGTGGAATGTACCAGCGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCACGATAAAACCGCCGCTTACCAGCAGGAGGtgat                                                       <  1:6256307/93‑1 (MQ=255)

                                  tCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGtgat                                                       <  1:3459563/93‑1 (MQ=255)

                                  tCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGtgat                                                       >  1:2400213/1‑93 (MQ=255)

                                  tCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGtgat                                                       >  2:246796/1‑93 (MQ=255)

                                      ggAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGGGATGaaa                                                   >  1:4551174/1‑93 (MQ=255)

                                          tGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAg                                               <  1:100077/93‑1 (MQ=255)

                                           gTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGt                                              <  1:55837/93‑1 (MQ=255)

                                           gTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGt                                              <  1:4078032/93‑1 (MQ=255)

                                               cAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTgcgc                                          <  2:4358755/93‑1 (MQ=255)

                                                  cGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAg                                       <  1:1542553/93‑1 (MQ=255)

                                                   gCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGt                                      <  2:4986546/93‑1 (MQ=255)

                                                   gCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGt                                      <  2:1940811/93‑1 (MQ=255)

                                                    cTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGtt                                     >  1:1550462/1‑93 (MQ=255)

                                                    cTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGtt                                     <  2:5750560/93‑1 (MQ=255)

                                                     tGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGttt                                    >  1:6336952/1‑93 (MQ=255)

                                                      gggCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTc                                   >  2:4864874/1‑93 (MQ=255)

                                                      gggCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTc                                   >  1:5689155/1‑93 (MQ=255)

                                                      gggCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTc                                   <  2:5472983/93‑1 (MQ=255)

                                                       ggCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCa                                  <  1:252028/93‑1 (MQ=255)

                                                        gCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCACGGTTCAc                                 >  2:4483481/1‑93 (MQ=255)

                                                         ccGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACt                                >  2:5070602/1‑93 (MQ=255)

                                                         ccGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACt                                >  1:245750/1‑93 (MQ=255)

                                                          cGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTa                               >  2:2894624/1‑93 (MQ=255)

                                                           gATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTag                              <  1:3340230/93‑1 (MQ=255)

                                                           gATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTag                              >  2:4709694/1‑93 (MQ=255)

                                                           gATCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTag                              <  1:4329820/93‑1 (MQ=255)

                                                            aTCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTaga                             >  1:2693812/1‑93 (MQ=255)

                                                            aTCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTaga                             <  2:1963527/93‑1 (MQ=255)

                                                            aTCTTCTGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTaga                             >  2:1562873/1‑93 (MQ=255)

                                                             tcttctGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTagag                            <  1:5145754/93‑1 (MQ=255)

                                                               ttctGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGGAATGAGTGCGCAAGTTTCACTAGAGtt                          >  1:1093621/1‑93 (MQ=255)

                                                               ttctGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGtt                          >  2:3465320/1‑93 (MQ=255)

                                                                tctGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTa                         >  2:3058145/1‑93 (MQ=255)

                                                                  tGAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACa                       <  2:6072726/93‑1 (MQ=255)

                                                                   gAGTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACAt                      <  1:686657/93‑1 (MQ=255)

                                                                    ggTAGCGAAAGCTGGCAGGAAGCGCTCGATAAACCCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATc                     <  1:83087/92‑1 (MQ=255)

                                                                     gTAGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGCAATGAGTGCGCAAGCTTCACTAGAGATACCTCa                    >  1:3525398/1‑93 (MQ=255)

                                                                       aGCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCAcc                  >  1:5448972/1‑93 (MQ=255)

                                                                        gCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCg                 >  2:2717967/1‑93 (MQ=255)

                                                                        gCGAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGGGCGCAAGTTTCACTAGAGTTACATCACCg                 >  1:1604538/1‑93 (MQ=255)

                                                                          gAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCa               >  2:1294594/1‑93 (MQ=255)

                                                                          gAAAGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCa               >  2:694702/1‑93 (MQ=255)

                                                                           aaaGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCAt              >  1:5538870/1‑93 (MQ=255)

                                                                           aaaGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCAt              >  2:2626649/1‑93 (MQ=255)

                                                                            aaGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCAtt             >  1:92820/1‑93 (MQ=255)

                                                                             aGCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTa            >  1:377543/1‑93 (MQ=255)

                                                                              gCTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAg           <  2:836060/93‑1 (MQ=255)

                                                                               cTGGCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGc          <  2:2009680/93‑1 (MQ=255)

                                                                                 ggCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCa        <  2:754990/93‑1 (MQ=255)

                                                                                  gCAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAg       >  2:5574171/1‑93 (MQ=255)

                                                                                   cAGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGt      <  1:2635156/93‑1 (MQ=255)

                                                                                    aGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGtt     >  1:2179649/1‑93 (MQ=255)

                                                                                    aGGAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGtt     <  1:1685561/93‑1 (MQ=255)

                                                                                     ggAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGttt    >  2:9082/1‑93 (MQ=255)

                                                                                     ggAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGttt    >  1:1968450/1‑93 (MQ=255)

                                                                                     ggAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGACAGttt    >  1:2976472/1‑93 (MQ=255)

                                                                                      gAAGCGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGTTtc   <  2:3144810/93‑1 (MQ=255)

GCATTACTAACTATATCTTTTCAGAAACTGCCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGTGCTCGATAAAACCGCCGCTTACCAGCAGGAGGTGATGAAATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGTTTCT  >  NC_000913/2668280‑2668459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`