BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,675,068	T→C	E438E (GAA→GAG)	yphE ←	putative ATP‑binding component of a transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,675,068	0	T	C	100.0%	766.4 / NA	235	E438E (GAA→GAG)	yphE	putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (122/113); total (122/113)

Only 100 of 239 total aligned reads displayed.

CGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGATT  >  NC_000913/2674978‑2675157

 ggCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCg                                                                                        >  2:4350935/1‑93 (MQ=255)

  gCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGa                                                                                       <  1:1933394/93‑1 (MQ=255)

    aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGata                                                                                     <  2:5551748/93‑1 (MQ=255)

    aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGata                                                                                     <  2:5165657/93‑1 (MQ=255)

    aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGata                                                                                     >  2:3651657/1‑93 (MQ=255)

     aCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGatat                                                                                    >  2:6244351/1‑93 (MQ=255)

      ctcctcCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGatata                                                                                   >  1:4998623/1‑92 (MQ=255)

      ctcctcCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATc                                                                                   <  2:5093567/93‑1 (MQ=255)

      ctcctcCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATc                                                                                   >  1:786921/1‑93 (MQ=255)

      ctcctcCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATc                                                                                   <  1:1474666/93‑1 (MQ=255)

       tcctccACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCg                                                                                  >  2:4995901/1‑93 (MQ=255)

       tcctccACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCAATATCg                                                                                  >  2:3749081/1‑93 (MQ=255)

        cctccACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGa                                                                                 <  1:70020/93‑1 (MQ=255)

         ctccACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAc                                                                                >  2:6091918/1‑93 (MQ=255)

         ctccACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAc                                                                                <  1:862394/93‑1 (MQ=255)

         ctccACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAc                                                                                >  1:3387839/1‑93 (MQ=255)

         ctccACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAc                                                                                >  1:1110320/1‑93 (MQ=255)

            cACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAcgcc                                                                             <  2:5856939/93‑1 (MQ=255)

             aCTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAcgccg                                                                            >  2:3979600/1‑93 (MQ=255)

             aCTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAcgccg                                                                            <  2:750266/93‑1 (MQ=255)

             aCTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAcgccg                                                                            >  1:5687940/1‑93 (MQ=255)

              cTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGAcgccgc                                                                           >  1:1302380/1‑93 (MQ=255)

               ttCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCcgcg                                                                          >  1:1566858/1‑93 (MQ=255)

                tCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCcgcgc                                                                         <  2:5401280/93‑1 (MQ=255)

                tCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCcgcgc                                                                         >  1:1260701/1‑93 (MQ=255)

                 cACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCcgcgcg                                                                        <  2:4451034/93‑1 (MQ=255)

                   cTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGtt                                                                      >  2:185911/1‑93 (MQ=255)

                   cTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGtt                                                                      <  1:1014487/93‑1 (MQ=255)

                   cTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGtt                                                                      <  2:5750066/93‑1 (MQ=255)

                     ggAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTgg                                                                    >  2:4106929/1‑93 (MQ=255)

                        gaTAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCtc                                                                 <  2:3421917/93‑1 (MQ=255)

                           aaaCACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCtcgtc                                                              <  1:273515/93‑1 (MQ=255)

                            aaCACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCtcgtcg                                                             >  1:547135/1‑93 (MQ=255)

                             acacCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGa                                                            >  2:2930462/1‑93 (MQ=255)

                               accacGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCgcgc                                                          >  1:3254363/1‑93 (MQ=255)

                               accacGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGc                                                          >  1:3208643/1‑93 (MQ=255)

                               accacGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGGTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGc                                                          >  1:2620495/1‑93 (MQ=255)

                                ccacGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGca                                                         >  1:6014116/1‑93 (MQ=255)

                                 cacGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGcaa                                                        >  1:1729707/1‑93 (MQ=255)

                                  acGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGcaac                                                       >  1:1626689/1‑93 (MQ=255)

                                  acGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGcaac                                                       >  2:1293808/1‑93 (MQ=255)

                                   cGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGcaaca                                                      >  1:5730494/1‑93 (MQ=255)

                                    gCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGcaacaa                                                     >  2:3159499/1‑93 (MQ=255)

                                    gCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGcaacaa                                                     >  2:1594954/1‑93 (MQ=255)

                                       ttttCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAAt                                                  >  2:1082025/1‑93 (MQ=255)

                                        tttCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATc                                                 <  2:4459881/93‑1 (MQ=255)

                                           ccTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctgg                                              >  1:2425222/1‑93 (MQ=255)

                                            cTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctggc                                             <  2:331531/93‑1 (MQ=255)

                                             ttCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctggct                                            >  1:4157394/1‑93 (MQ=255)

                                             ttCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctggct                                            <  2:5927290/93‑1 (MQ=255)

                                             ttCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctggct                                            >  2:1114752/1‑93 (MQ=255)

                                              tCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctggctg                                           <  1:5278665/93‑1 (MQ=255)

                                               cGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATctggctgg                                          <  1:5786978/93‑1 (MQ=255)

                                                ggCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCtggctggc                                         >  1:2591617/1‑93 (MQ=255)

                                                   agccagcTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGgcagc                                      >  2:4789447/1‑93 (MQ=255)

                                                    gccagcTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGgcagca                                     >  2:2412656/1‑93 (MQ=255)

                                                     ccagcTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCAt                                    >  1:2935222/1‑93 (MQ=255)

                                                       agcTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAg                                  >  2:4915150/1‑93 (MQ=255)

                                                         cTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAc                                <  2:5960490/93‑1 (MQ=255)

                                                         cTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAc                                <  1:6670168/93‑1 (MQ=255)

                                                         cTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCACAGAc                                >  2:5610566/1‑93 (MQ=255)

                                                           cACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAccc                              <  2:3076831/93‑1 (MQ=255)

                                                           cACGGACAATACGGTAAATCGGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGAccc                              <  1:1095571/93‑1 (MQ=255)

                                                              ggACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAAc                           <  2:6366694/93‑1 (MQ=255)

                                                               gACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACg                          >  2:217461/1‑93 (MQ=255)

                                                                aCAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGa                         >  1:398783/1‑93 (MQ=255)

                                                                 cAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGAc                        <  2:1994516/93‑1 (MQ=255)

                                                                   aTACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCg                      >  1:2009239/1‑93 (MQ=255)

                                                                   aTACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCg                      >  2:1423042/1‑93 (MQ=255)

                                                                    tACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGa                     <  2:6338065/93‑1 (MQ=255)

                                                                    tACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGa                     <  1:982245/93‑1 (MQ=255)

                                                                      cGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATc                   >  1:5126718/1‑93 (MQ=255)

                                                                      cGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATc                   <  1:6362118/93‑1 (MQ=255)

                                                                      cGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATc                   <  2:4026407/93‑1 (MQ=255)

                                                                      cGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCCTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATc                   <  2:804350/93‑1 (MQ=255)

                                                                        gTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATcac                 >  1:1650698/1‑93 (MQ=255)

                                                                           aaTCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATcaccac              >  2:4315929/1‑93 (MQ=255)

                                                                           aaTCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATcaccac              >  2:2713809/1‑93 (MQ=255)

                                                                            aTCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACt             >  1:4582978/1‑93 (MQ=255)

                                                                            aTCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACt             >  2:1492894/1‑93 (MQ=255)

                                                                            aTCTGCTGTTTGGCCTCGATAGCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACt             <  1:2850249/93‑1 (MQ=255)

                                                                             tCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATATACCCAACGGCCGATCACCACtt            >  2:35334/1‑93 (MQ=255)

                                                                              ctgctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACttt           >  1:4696194/1‑93 (MQ=255)

                                                                               tgctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACtttt          >  2:6116223/1‑93 (MQ=255)

                                                                               tgctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACtttt          <  2:172459/93‑1 (MQ=255)

                                                                                gctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCACCGACCGATCACCACttttt         <  1:6570785/93‑1 (MQ=255)

                                                                                gctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACttttt         <  1:6255676/93‑1 (MQ=255)

                                                                                gctgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACttttt         <  2:1838644/93‑1 (MQ=255)

                                                                                  tgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGc       >  1:3728712/1‑93 (MQ=255)

                                                                                  tgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGc       >  1:2285812/1‑93 (MQ=255)

                                                                                  tgTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGc       <  2:165983/93‑1 (MQ=255)

                                                                                   gTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCt      <  1:5553416/93‑1 (MQ=255)

                                                                                    tttGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTg     >  1:3074089/1‑93 (MQ=255)

                                                                                     ttGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGa    >  1:3907134/1‑93 (MQ=255)

                                                                                      tGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAt   >  1:2178424/1‑93 (MQ=255)

                                                                                      tGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAt   <  2:5418052/93‑1 (MQ=255)

                                                                                      tGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAt   >  1:1269469/1‑93 (MQ=255)

                                                                                       ggCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAtt  >  2:2777471/1‑93 (MQ=255)

                                                                                       ggCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAtt  <  2:5219743/93‑1 (MQ=255)

                                                                                       ggCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGAtt  <  2:1002128/93‑1 (MQ=255)

CGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGCAACAAAATCTGGCTGGCAGCATAGACCCAACGACCGATCACCACTTTTTGCTGATT  >  NC_000913/2674978‑2675157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`