BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,696,865	T→C	P53P (CCT→CCC)	yfhH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,696,865	0	T	C	99.6%	716.3 / NA	222	P53P (CCT→CCC)	yfhH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (119/102); total (119/103)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 229 total aligned reads displayed.

GATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGG  >  NC_000913/2696776‑2696954

gATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGAt                                                                                        <  1:6308236/93‑1 (MQ=255)

gATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGAt                                                                                        <  2:2238911/93‑1 (MQ=255)

gATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGAt                                                                                        >  1:2619564/1‑93 (MQ=255)

 atatGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATa                                                                                       >  1:5313622/1‑93 (MQ=255)

 atagGAACGGCTTACTTCGTACCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATa                                                                                       <  1:2390522/93‑1 (MQ=255)

  tatGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATAc                                                                                      <  2:403841/93‑1 (MQ=255)

  tatGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATAc                                                                                      <  2:2430588/93‑1 (MQ=255)

   atGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATaag                                                                                     >  1:1401427/1‑91 (MQ=255)

    tGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACgg                                                                                    >  1:4495931/1‑93 (MQ=255)

     gAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGgc                                                                                   <  2:1389141/93‑1 (MQ=255)

     gAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGgc                                                                                   <  2:459547/93‑1 (MQ=255)

     gAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGgc                                                                                   <  2:6243695/93‑1 (MQ=255)

     gAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGgc                                                                                   <  2:6638838/93‑1 (MQ=255)

       aCGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTAAAACCCGATACGgcgc                                                                                 >  1:5235463/1‑93 (MQ=255)

         ggCTTACTTCGTATCCGTCAGCGTTACCAGGAGCTTGCACAGAGAGAT‑AAAAAACTGGCGGATTATATGCTGCTACAACCCGATAAGgcgcgc                                                                               >  1:1914476/1‑93 (MQ=255)

          gCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGcc                                                                              >  1:2460566/1‑93 (MQ=255)

          gCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGcc                                                                              >  1:4521980/1‑93 (MQ=255)

            ttACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCAt                                                                            <  2:6070763/93‑1 (MQ=255)

             tACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCAtt                                                                           <  2:4677360/93‑1 (MQ=255)

                ttCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTaa                                                                        <  1:5301969/93‑1 (MQ=255)

                ttCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTaa                                                                        >  2:4362633/1‑93 (MQ=255)

                 tCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAg                                                                       >  1:6428439/1‑93 (MQ=255)

                  cGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGc                                                                      >  2:1214602/1‑93 (MQ=255)

                  cGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGc                                                                      <  2:6346961/93‑1 (MQ=255)

                   gTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGct                                                                     >  2:1610631/1‑93 (MQ=255)

                    tATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGctc                                                                    >  1:4943760/1‑93 (MQ=255)

                    tATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGctc                                                                    >  1:282567/1‑93 (MQ=255)

                     aTCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGctct                                                                   <  1:355385/93‑1 (MQ=255)

                      tCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCtctc                                                                  >  2:7142/1‑93 (MQ=255)

                      tCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCtctc                                                                  <  2:5779697/93‑1 (MQ=255)

                       ccGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCa                                                                 >  1:2529472/1‑93 (MQ=255)

                       ccGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCa                                                                 <  2:3500476/93‑1 (MQ=255)

                        cGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAg                                                                >  1:960115/1‑93 (MQ=255)

                         gTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGc                                                               <  1:194698/93‑1 (MQ=255)

                         gTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGc                                                               >  1:135556/1‑93 (MQ=255)

                             gCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACt                                                           <  2:3676972/93‑1 (MQ=255)

                                   ccAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCaa                                                     >  1:6047435/1‑93 (MQ=255)

                                    cAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAAc                                                    >  1:1251686/1‑93 (MQ=255)

                                     aGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACg                                                   >  2:2183125/1‑93 (MQ=255)

                                      ggggCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGa                                                  >  1:3745619/1‑93 (MQ=255)

                                      ggggCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGa                                                  >  1:6142186/1‑93 (MQ=255)

                                      ggggCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGa                                                  <  2:4801497/93‑1 (MQ=255)

                                         gCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGc                                               <  2:2639173/93‑1 (MQ=255)

                                         gCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGc                                               <  1:3289823/93‑1 (MQ=255)

                                            tGCCCAGAGTGATAAAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCg                                             >  2:847463/1‑93 (MQ=255)

                                             gCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGa                                           <  2:1274114/93‑1 (MQ=255)

                                             gCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGa                                           <  2:880189/93‑1 (MQ=255)

                                               ccAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagt                                         >  2:5509694/1‑93 (MQ=255)

                                                 agagTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtca                                       <  2:1062188/93‑1 (MQ=255)

                                                 agagTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtca                                       >  2:2922385/1‑93 (MQ=255)

                                                   agTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagt                                     >  1:5252197/1‑93 (MQ=255)

                                                    gTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtc                                    >  2:4319094/1‑93 (MQ=255)

                                                    gTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtc                                    >  2:5316565/1‑93 (MQ=255)

                                                    gTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagta                                    >  2:4096720/1‑92 (MQ=255)

                                                     tGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtca                                   <  1:5823515/93‑1 (MQ=255)

                                                      gAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtcag                                  >  1:2266035/1‑93 (MQ=255)

                                                       aT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtcagt                                 <  1:5463686/93‑1 (MQ=255)

                                                       aT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtcagt                                 >  1:3800489/1‑93 (MQ=255)

                                                         gaaaaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGagtcagtcagtc                                <  2:2771386/92‑1 (MQ=255)

                                                          aaaaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTcc                               >  1:3434985/1‑93 (MQ=255)

                                                          aaaaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTcc                               <  1:3959850/93‑1 (MQ=255)

                                                          aaaaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTcc                               >  2:1412923/1‑93 (MQ=255)

                                                          aaaaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTcc                               >  2:3415765/1‑93 (MQ=255)

                                                           aaaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCa                              <  1:5750818/93‑1 (MQ=255)

                                                           aaaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCa                              <  1:2683478/93‑1 (MQ=255)

                                                            aaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCac                             >  1:3893529/1‑92 (MQ=255)

                                                            aaaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAg                             >  2:2041214/1‑93 (MQ=255)

                                                             aaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGc                            >  1:5093283/1‑93 (MQ=255)

                                                             aaaCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGc                            >  2:6715460/1‑93 (MQ=255)

                                                               aCTGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGcgt                          <  2:5144145/93‑1 (MQ=255)

                                                                 tGGCGGATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGcgtcg                        <  1:6493008/93‑1 (MQ=255)

                                                                    cGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGa                     <  2:3877870/93‑1 (MQ=255)

                                                                     ggATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGaa                    <  1:6489993/93‑1 (MQ=255)

                                                                     ggATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGaa                    <  2:3185276/93‑1 (MQ=255)

                                                                     ggATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGaa                    >  2:6614825/1‑93 (MQ=255)

                                                                     ggATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGaa                    <  2:492836/93‑1 (MQ=255)

                                                                     ggATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGaa                    <  1:5348389/93‑1 (MQ=255)

                                                                      gATTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAg                   <  2:1786736/93‑1 (MQ=255)

                                                                       aTTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGACGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGt                  >  2:5383842/1‑93 (MQ=255)

                                                                       aTTATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGt                  <  2:6429485/93‑1 (MQ=255)

                                                                         tATCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTc                <  1:3121848/93‑1 (MQ=255)

                                                                           tCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTcgc              >  2:1756893/1‑93 (MQ=255)

                                                                           tCTGCTGCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTcgc              >  2:4902365/1‑93 (MQ=255)

                                                                            ctgctgCTACAGCCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTcgcg             <  2:5907208/93‑1 (MQ=255)

                                                                            ctgctgCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTcgcg             <  2:299534/93‑1 (MQ=255)

                                                                            ctgctgCTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTcgcg             <  2:5639719/93‑1 (MQ=255)

                                                                             tgctgcTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCgcgc            >  1:3045766/1‑93 (MQ=255)

                                                                             tgctgcTACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCgcgc            >  1:4410507/1‑93 (MQ=255)

                                                                              gctgctACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCa           >  1:4176535/1‑93 (MQ=255)

                                                                                tgctACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCaaa         >  1:1042233/1‑93 (MQ=255)

                                                                                  ctACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCaaaaa       >  1:3711508/1‑93 (MQ=255)

                                                                                  ctACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCaaaaa       <  2:5648926/93‑1 (MQ=255)

                                                                                  ctACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCaaaaa       <  2:2557307/93‑1 (MQ=255)

                                                                                  ctACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCaaaaa       <  1:6621542/93‑1 (MQ=255)

                                                                                   tACAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAAc      <  2:5005876/93‑1 (MQ=255)

                                                                                    aCAACCCGATGCGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACt     <  1:1764353/93‑1 (MQ=255)

                                                                                     cAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTc    >  2:6131466/1‑93 (MQ=255)

                                                                                     cAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTc    <  2:5961803/93‑1 (MQ=255)

                                                                                     cAACCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTc    <  2:6469445/93‑1 (MQ=255)

                                                                                       aCCCGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCgg  >  2:2875941/1‑93 (MQ=255)

GATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAT‑AAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGG  >  NC_000913/2696776‑2696954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`