BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,732,361	T→C	T232A (ACC→GCC)	yfiH ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,732,361	0	T	C	99.5%	611.7 / NA	189	T232A (ACC→GCC)	yfiH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (78/110); minor base G (1/0); total (79/110)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

AGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCC  >  NC_000913/2732271‑2732449

aGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCt                                                                                        <  2:3921771/93‑1 (MQ=255)

aGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCt                                                                                        <  2:3133419/93‑1 (MQ=255)

aGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCt                                                                                        <  2:2820396/93‑1 (MQ=255)

aGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCt                                                                                        <  1:636299/93‑1 (MQ=255)

aGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCt                                                                                        <  1:552903/93‑1 (MQ=255)

aGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCt                                                                                        >  1:3149943/1‑93 (MQ=255)

 gATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCtt                                                                                       >  2:5101800/1‑93 (MQ=255)

   ttAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGt                                                                                     >  1:4740028/1‑93 (MQ=255)

    tAATGTGAAAAGAAAACATCGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGCCCGGT‑GCCTTGTcg                                                                                   <  1:2307679/93‑1 (MQ=255)

         tgAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGcgac                                                                               <  2:1445073/93‑1 (MQ=255)

          gaaaagaaaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGcgacg                                                                              >  2:4867576/1‑93 (MQ=255)

          gaaaagaaaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGcgacg                                                                              >  1:5118888/1‑93 (MQ=255)

          gaaaagaaaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATAGCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGcgacg                                                                              <  1:1929494/93‑1 (MQ=255)

           aaaagaaaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGcgacga                                                                             <  2:1201042/93‑1 (MQ=255)

           aaaagaaaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGcgacga                                                                             >  1:5319877/1‑93 (MQ=255)

              agaaaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATaa                                                                          <  1:5244494/93‑1 (MQ=255)

              agaaaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATaa                                                                          <  1:6044490/93‑1 (MQ=255)

              agaaaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATaa                                                                          <  1:4134403/93‑1 (MQ=255)

              agaaaaTCACGCGCACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATaa                                                                          >  1:348769/1‑93 (MQ=255)

                aaaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAaga                                                                        >  2:6554428/1‑93 (MQ=255)

                 aaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAagag                                                                       <  2:261402/93‑1 (MQ=255)

                 aaaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAagag                                                                       >  2:5927343/1‑93 (MQ=255)

                  actCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAgaga                                                                      <  2:2099068/91‑1 (MQ=255)

                  aaTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAgaga                                                                      >  2:1960948/1‑93 (MQ=255)

                   aTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGagaa                                                                     >  1:818107/1‑93 (MQ=255)

                   aTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGagaa                                                                     <  2:3756566/93‑1 (MQ=255)

                   aTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGagaa                                                                     >  1:1687100/1‑93 (MQ=255)

                    tCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAgaggag                                                                    <  2:2647371/93‑1 (MQ=255)

                     cACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGagaaga                                                                   <  1:3801192/93‑1 (MQ=255)

                     cACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGagaaga                                                                   <  1:931489/93‑1 (MQ=255)

                     cACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGagaaga                                                                   <  1:3428510/93‑1 (MQ=255)

                     cACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGagaaga                                                                   >  1:4771802/1‑93 (MQ=255)

                      aCGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAgaagaa                                                                  <  1:503002/93‑1 (MQ=255)

                          gTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGtc                                                              <  2:2327968/93‑1 (MQ=255)

                            aCCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGtctc                                                            >  1:4808425/1‑93 (MQ=255)

                             ccGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCa                                                           <  1:1180519/93‑1 (MQ=255)

                              cGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCAt                                                          >  1:6338746/1‑93 (MQ=255)

                              cGGATCGTCTTGATTCTTTAGATTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCAt                                                          <  2:2547314/93‑1 (MQ=255)

                               ggATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCAtt                                                         <  1:2950459/93‑1 (MQ=255)

                               ggATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCAtt                                                         >  2:3986438/1‑93 (MQ=255)

                                gATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCAttt                                                        >  1:626136/1‑93 (MQ=255)

                                 aTCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCAtttt                                                       >  2:5260267/1‑93 (MQ=255)

                                  tCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTc                                                      <  2:6145589/93‑1 (MQ=255)

                                   cGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTcc                                                     >  2:6002951/1‑93 (MQ=255)

                                   cGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTcc                                                     <  1:2846348/93‑1 (MQ=255)

                                   cGTCTTGATTCTTTAGGTGATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTcc                                                     <  1:1352629/93‑1 (MQ=255)

                                    gTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg                                                    >  2:3550920/1‑93 (MQ=255)

                                    gTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg                                                    >  1:2592138/1‑93 (MQ=255)

                                    gTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg                                                    <  1:2391838/93‑1 (MQ=255)

                                    gTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg                                                    >  1:5306173/1‑93 (MQ=255)

                                    gTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg                                                    >  1:5925743/1‑93 (MQ=255)

                                      cTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGATAAGAAAGTCTCATTTTCCGta                                                  >  1:515528/1‑93 (MQ=255)

                                       ttGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGtat                                                 >  2:4113458/1‑93 (MQ=255)

                                        tGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGtata                                                <  1:678937/93‑1 (MQ=255)

                                        tGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGtata                                                <  1:1593692/93‑1 (MQ=255)

                                         gATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTatat                                               >  2:4291787/1‑93 (MQ=255)

                                          aTTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATg                                              >  2:49906/1‑93 (MQ=255)

                                           ttCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGt                                             <  2:677949/93‑1 (MQ=255)

                                               ttAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACaa                                         <  1:4289215/93‑1 (MQ=255)

                                                 aGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCGTTTTCCGTATATGTACAACg                                       >  2:4435699/1‑93 (MQ=255)

                                                 aGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACg                                       <  2:1523865/93‑1 (MQ=255)

                                                   gTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGt                                     <  2:5399882/93‑1 (MQ=255)

                                                    ttATATCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTc                                    <  1:3202748/93‑1 (MQ=255)

                                                     tataTCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTcg                                   >  1:3585108/1‑93 (MQ=255)

                                                      atatCAGCCAAATGAACCTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTcgc                                  <  1:4399138/93‑1 (MQ=255)

                                                      atatCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTcgc                                  >  2:4057349/1‑93 (MQ=255)

                                                      atatCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTcgc                                  <  2:1356333/93‑1 (MQ=255)

                                                       tatCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTcgcc                                 >  2:1285891/1‑93 (MQ=255)

                                                       tatCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTcgcc                                 <  2:3452154/93‑1 (MQ=255)

                                                         tCAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTcgccgc                               <  1:5084290/93‑1 (MQ=255)

                                                          cAGCCAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCgccgcc                              <  1:4223408/93‑1 (MQ=255)

                                                             ccAAATGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGaa                           >  2:216973/1‑93 (MQ=255)

                                                                 aTGAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAAtt                       >  1:2069447/1‑93 (MQ=255)

                                                                   gAAACTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTCTATGTACAACGGTCGCCGCCGAAAATTTg                     >  2:1681030/1‑93 (MQ=255)

                                                                     aaCTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCt                   >  2:3283763/1‑93 (MQ=255)

                                                                      aCTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTc                  >  1:5822241/1‑93 (MQ=255)

                                                                       cTTGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCa                 >  1:2756854/1‑93 (MQ=255)

                                                                        ttGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCaa                >  2:565886/1‑93 (MQ=255)

                                                                        ttGCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCaa                <  2:1759272/93‑1 (MQ=255)

                                                                          gCCATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAaca              >  2:3372692/1‑93 (MQ=255)

                                                                           ccATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAacac             <  1:3762204/93‑1 (MQ=255)

                                                                           ccATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAacac             <  1:748261/93‑1 (MQ=255)

                                                                            cATACGACCGGTGTCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACAcc            <  2:1137509/93‑1 (MQ=255)

                                                                            cATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACAcc            <  2:4780399/93‑1 (MQ=255)

                                                                            cATACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACAcc            >  2:4684585/1‑93 (MQ=255)

                                                                             aTACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACAccc           >  2:1708768/1‑93 (MQ=255)

                                                                             aTACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACAccc           <  2:626554/93‑1 (MQ=255)

                                                                             aTACGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTAAACAccc           >  1:3003658/1‑93 (MQ=255)

                                                                               aCGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCAc         >  2:2774872/1‑93 (MQ=255)

                                                                                cGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACg        <  1:2556191/93‑1 (MQ=255)

                                                                                cGACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACg        >  2:6586293/1‑93 (MQ=255)

                                                                                 gACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGt       >  1:983064/1‑93 (MQ=255)

                                                                                 gACCGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGt       <  2:794970/93‑1 (MQ=255)

                                                                                   ccGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTc     <  1:5405825/93‑1 (MQ=255)

                                                                                   ccGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTc     >  2:2132672/1‑93 (MQ=255)

                                                                                   ccGGTGGCCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTc     >  2:4528801/1‑93 (MQ=255)

                                                                                     ggtggcCTTGTGGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGGACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGc   <  2:4695319/93‑1 (MQ=255)

                                                                                      gtggcCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGcc  >  1:3875277/1‑93 (MQ=255)

                                                                                      gtggcCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGcc  >  1:5675104/1‑93 (MQ=255)

                                                                                      gtggcCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGcc  >  2:1377789/1‑93 (MQ=255)

AGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGTATATGTACAACGGTCGCCGCCGAAAATTTGCTCAACACCCACGTTCGCC  >  NC_000913/2732271‑2732449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`