BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	321,039	G→A	D70N (GAC→AAC)	ykgE →	putative dehydrogenase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	321,039	0	G	A	100.0%	747.0 / NA	198	D70N (GAC→AAC)	ykgE	putative dehydrogenase subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (116/82); total (116/82)

Only 100 of 209 total aligned reads displayed.

GGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCATCA  >  NC_000913/320949‑321128

 gATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAacaacg                                                                                        <  2:4889842/93‑1 (MQ=255)

 gATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAacaacg                                                                                        >  2:2956037/1‑93 (MQ=255)

  aTGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAAcaacga                                                                                       >  2:4055861/1‑93 (MQ=255)

  aTGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAAcaacga                                                                                       >  1:2395951/1‑93 (MQ=255)

     ctgcGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATcc                                                                                    <  2:2102485/93‑1 (MQ=255)

      tgcGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATccc                                                                                   >  1:3065033/1‑93 (MQ=255)

      tgcGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATccc                                                                                   >  2:3565873/1‑93 (MQ=255)

       gcGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCa                                                                                  <  2:5297087/93‑1 (MQ=255)

       gcGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCa                                                                                  <  2:1528747/93‑1 (MQ=255)

       gcGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCa                                                                                  <  2:6657579/93‑1 (MQ=255)

       gcGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCa                                                                                  <  1:6576035/93‑1 (MQ=255)

         ggTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCatt                                                                                >  1:5078446/1‑93 (MQ=255)

           tCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCattat                                                                              <  1:1478126/93‑1 (MQ=255)

                cTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCAc                                                                         <  2:229171/93‑1 (MQ=255)

                 tGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCAcc                                                                        >  1:440072/1‑93 (MQ=255)

                  gCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCg                                                                       >  1:3570795/1‑93 (MQ=255)

                    gATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggc                                                                     <  2:1418244/93‑1 (MQ=255)

                    gATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggc                                                                     >  1:3131472/1‑93 (MQ=255)

                     aTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGTAGGATAACAACGATCCCATTATTTCACCggct                                                                    <  1:3402447/93‑1 (MQ=255)

                     aTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggct                                                                    >  1:6139665/1‑93 (MQ=255)

                     aTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggct                                                                    <  1:2649158/93‑1 (MQ=255)

                     aTCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggct                                                                    <  1:3925172/93‑1 (MQ=255)

                      tCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggctg                                                                   <  2:1203918/93‑1 (MQ=255)

                      tCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggctg                                                                   <  1:42050/93‑1 (MQ=255)

                      tCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggctg                                                                   >  2:5031661/1‑93 (MQ=255)

                       cAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggctgg                                                                  >  1:2849002/1‑93 (MQ=255)

                       cAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggctgg                                                                  >  2:5742559/1‑93 (MQ=255)

                       cAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggctgg                                                                  >  1:5191224/1‑93 (MQ=255)

                         aTAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCggctggcc                                                                >  1:5660595/1‑92 (MQ=255)

                          tAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGctc                                                               >  2:6549389/1‑93 (MQ=255)

                           aGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGctct                                                              <  2:2857707/93‑1 (MQ=255)

                           aGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGctct                                                              >  1:4965818/1‑93 (MQ=255)

                           aGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGctct                                                              <  2:956512/93‑1 (MQ=255)

                           aGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGctct                                                              <  1:5421575/93‑1 (MQ=255)

                           aGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGctct                                                              <  2:5872831/93‑1 (MQ=255)

                            gCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCTCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCtt                                                             <  2:2421243/93‑1 (MQ=255)

                            gCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCtt                                                             >  1:2641504/1‑93 (MQ=255)

                             cGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTg                                                            >  2:1570475/1‑93 (MQ=255)

                              ggTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGc                                                           >  2:6027652/1‑93 (MQ=255)

                               gTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGcc                                                          >  1:3249721/1‑92 (MQ=255)

                                 tataTCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCAcc                                                        <  1:380279/93‑1 (MQ=255)

                                   tatCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTGCACCGGCTGGCTCTTGCACCTa                                                      >  2:5375046/1‑93 (MQ=255)

                                    atCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTAt                                                     >  1:1303545/1‑93 (MQ=255)

                                     tCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATg                                                    <  1:1679121/93‑1 (MQ=255)

                                      cAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGc                                                   >  1:158839/1‑93 (MQ=255)

                                       aaaGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGcc                                                  <  1:3176902/93‑1 (MQ=255)

                                        aagaagCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCg                                                 >  1:753053/1‑93 (MQ=255)

                                          gaagCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTa                                               <  2:1855132/93‑1 (MQ=255)

                                            agCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTaaa                                             >  2:1089013/1‑93 (MQ=255)

                                             gCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTaaaa                                            <  2:4255549/93‑1 (MQ=255)

                                               gATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAg                                          >  2:4417066/1‑93 (MQ=255)

                                                aTTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGt                                         >  1:1749029/1‑93 (MQ=255)

                                                 ttCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGtt                                        >  1:474350/1‑93 (MQ=255)

                                                 ttCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGtt                                        <  1:4852544/93‑1 (MQ=255)

                                                  tCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTa                                       <  2:5975442/93‑1 (MQ=255)

                                                   ccAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTAc                                      <  1:646461/93‑1 (MQ=255)

                                                    cAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTAcc                                     <  2:6204335/93‑1 (MQ=255)

                                                     aGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTAccc                                    >  1:1310744/1‑93 (MQ=255)

                                                      gggATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCg                                   >  1:5901202/1‑93 (MQ=255)

                                                      gggATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCg                                   >  2:1048166/1‑93 (MQ=255)

                                                      gggATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCg                                   >  1:899990/1‑93 (MQ=255)

                                                      gggATGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCAACTATGCCGTAAAAAGTTAccca                                   >  2:6454033/1‑92 (MQ=255)

                                                         aTGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACg                                >  2:4566285/1‑93 (MQ=255)

                                                         aTGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACg                                >  2:3500840/1‑93 (MQ=255)

                                                         aTGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACg                                >  2:3246673/1‑93 (MQ=255)

                                                         aTGAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACg                                >  2:2772379/1‑93 (MQ=255)

                                                           gAAAAATCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTa                              >  2:1155400/1‑93 (MQ=255)

                                                             aaaaTCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATc                            <  1:3224894/93‑1 (MQ=255)

                                                             aaaaTCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATc                            >  1:5825994/1‑93 (MQ=255)

                                                              aaaTCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCt                           <  1:1065425/93‑1 (MQ=255)

                                                                aTCTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTgg                         <  1:2908840/93‑1 (MQ=255)

                                                                 tCTGATCGCCCCACTGGAGGATAACAACGATCCCATTATTTCACCGGCGGGCTCCTGCAGCTATGCCGCAAAAAGCTACCCGACGGAGCTggg                        >  1:6706464/1‑92 (MQ=255)

                                                                  cTGATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCg                       <  1:6375733/93‑1 (MQ=255)

                                                                    gATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGa                     <  2:3186560/93‑1 (MQ=255)

                                                                    gATCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGa                     >  2:6166739/1‑93 (MQ=255)

                                                                     aTCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGAt                    >  1:534842/1‑93 (MQ=255)

                                                                      tCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATg                   >  1:2292255/1‑93 (MQ=255)

                                                                      tCGCCGCACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATg                   >  2:5180976/1‑93 (MQ=255)

                                                                       cgctgcACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGa                  <  1:1966022/93‑1 (MQ=255)

                                                                          cgcACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAAcc               <  2:2737944/93‑1 (MQ=255)

                                                                          cgcACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAAcc               >  2:3438721/1‑93 (MQ=255)

                                                                          cgcACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAAcc               >  2:3054114/1‑93 (MQ=255)

                                                                           gcACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCt              <  2:6388150/93‑1 (MQ=255)

                                                                           gcACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCt              >  2:6496217/1‑93 (MQ=255)

                                                                            cACTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTg             >  2:4914301/1‑93 (MQ=255)

                                                                             aCTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGa            >  2:3649011/1‑93 (MQ=255)

                                                                              cTGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGaa           >  1:1931697/1‑93 (MQ=255)

                                                                               tGGAGTATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAAt          <  1:2500234/93‑1 (MQ=255)

                                                                               tGGAGGATAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAAt          <  2:6270891/93‑1 (MQ=255)

                                                                                ggaggaTAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATg         <  1:6270808/93‑1 (MQ=255)

                                                                                ggaggaTAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATg         <  1:3680422/93‑1 (MQ=255)

                                                                                 gaggaTAACAACGATCCCATTATTTCACCGGCTGGGTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATgg        >  2:6311921/1‑93 (MQ=255)

                                                                                    gaTAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCa     <  2:735379/93‑1 (MQ=255)

                                                                                    gaTAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCa     >  1:6425503/1‑93 (MQ=255)

                                                                                    gaTAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCa     <  2:4001626/93‑1 (MQ=255)

                                                                                     aTAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCAt    >  2:3863548/1‑93 (MQ=255)

                                                                                     aTAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCAt    >  2:382480/1‑93 (MQ=255)

                                                                                      tAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGGATc   >  2:542251/1‑93 (MQ=255)

                                                                                      tAACAACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCATc   >  2:20365/1‑93 (MQ=255)

                                                                                       aacaacGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCATCa  >  1:6054183/1‑93 (MQ=255)

GGATGCTGCGGTCAGCCTGCGATCAATAGCGGTTATATCAAAGAAGCGATTCCAGGGATGAAAAATCTGATCGCCGCACTGGAGGATAACGACGATCCCATTATTTCACCGGCTGGCTCTTGCACCTATGCCGTAAAAAGTTACCCGACGTATCTGGCGGATGAACCTGAATGGGCATCA  >  NC_000913/320949‑321128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`