BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,746,200	T→C	S73S (AGT→AGC)	b2611 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,746,200	0	T	C	99.6%	806.6 / NA	246	S73S (AGT→AGC)	b2611	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (130/115); total (130/116)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

CTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAA  >  NC_000913/2746110‑2746289

  gCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTTAGCTtga                                                                                       <  2:1235293/93‑1 (MQ=255)

  gCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtga                                                                                       <  1:6054761/93‑1 (MQ=255)

   ccACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtgat                                                                                      <  2:4007549/93‑1 (MQ=255)

     aCGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtgatga                                                                                    >  1:3475961/1‑93 (MQ=255)

     aCGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtgatga                                                                                    >  2:4745020/1‑93 (MQ=255)

     aCGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTtgatga                                                                                    >  2:3825616/1‑93 (MQ=255)

        cAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCt                                                                                 >  1:6415470/1‑93 (MQ=255)

         aaTCGCTCTGGAAGCCCGCATCCTGCTCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTg                                                                                <  1:5389008/93‑1 (MQ=255)

          aTCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGt                                                                               >  2:5775149/1‑93 (MQ=255)

            cGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTAc                                                                             >  1:4073446/1‑93 (MQ=255)

            cGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTAc                                                                             <  2:2720483/93‑1 (MQ=255)

             gCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACg                                                                            >  2:754688/1‑93 (MQ=255)

             gCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACg                                                                            <  1:2370755/93‑1 (MQ=255)

               tctGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGt                                                                          <  2:1859087/93‑1 (MQ=255)

               tctGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGt                                                                          <  2:4931492/93‑1 (MQ=255)

                ctGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTa                                                                         >  1:3317160/1‑93 (MQ=255)

                  ggAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAAt                                                                       <  2:5889779/93‑1 (MQ=255)

                  ggAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAAt                                                                       >  1:3886371/1‑93 (MQ=255)

                      gCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGAcc                                                                   <  2:2128069/93‑1 (MQ=255)

                      gCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGAcc                                                                   <  1:1227101/93‑1 (MQ=255)

                       cccGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCa                                                                  <  1:6364796/93‑1 (MQ=255)

                        ccGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCAt                                                                 >  1:3949914/1‑93 (MQ=255)

                        ccGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCAt                                                                 <  1:6351605/93‑1 (MQ=255)

                        ccGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCAt                                                                 >  1:5981074/1‑93 (MQ=255)

                         cGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCAtt                                                                >  1:1020667/1‑93 (MQ=255)

                         cGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCAtt                                                                <  1:1746211/93‑1 (MQ=255)

                         cGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCAtt                                                                <  2:4170415/93‑1 (MQ=255)

                          gCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATtg                                                               >  2:3441294/1‑93 (MQ=255)

                           cATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATtgt                                                              >  2:247951/1‑93 (MQ=255)

                           cATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTGCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATtgt                                                              <  1:853454/93‑1 (MQ=255)

                             tCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTgg                                                            >  1:3500925/1‑93 (MQ=255)

                             tCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTgg                                                            >  1:5178140/1‑93 (MQ=255)

                             tCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTgg                                                            <  1:4191593/93‑1 (MQ=255)

                              ccTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGc                                                           <  2:696883/93‑1 (MQ=255)

                              ccTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGc                                                           <  2:539751/93‑1 (MQ=255)

                                 gCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTtc                                                        <  1:2038499/93‑1 (MQ=255)

                                 gCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTtc                                                        <  2:5474145/93‑1 (MQ=255)

                                 gCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTtc                                                        <  2:403595/93‑1 (MQ=255)

                                   ccGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTtctc                                                      <  1:5540340/93‑1 (MQ=255)

                                   ccGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTtctc                                                      >  1:6579188/1‑93 (MQ=255)

                                    cGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCg                                                     >  1:6097896/1‑93 (MQ=255)

                                     gACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGc                                                    >  2:2528013/1‑93 (MQ=255)

                                       cGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCaa                                                  >  1:183716/1‑93 (MQ=255)

                                        ggTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAAt                                                 <  2:65093/93‑1 (MQ=255)

                                        ggTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAAt                                                 >  2:6692050/1‑93 (MQ=255)

                                        ggTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAAt                                                 <  1:956820/93‑1 (MQ=255)

                                         gTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATc                                                >  2:4109527/1‑93 (MQ=255)

                                         gTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATc                                                <  2:4405380/93‑1 (MQ=255)

                                         gTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATc                                                >  2:3504932/1‑93 (MQ=255)

                                          tGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCg                                               >  2:3538049/1‑93 (MQ=255)

                                             tAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtgg                                            <  2:1779687/93‑1 (MQ=255)

                                               gCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCCATCGttgct                                          >  2:6340983/1‑93 (MQ=255)

                                                 ggACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtggctgg                                        >  2:2700467/1‑93 (MQ=255)

                                                  gACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtggctggc                                       <  2:4869621/93‑1 (MQ=255)

                                                  gACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtggctggc                                       <  1:3797913/93‑1 (MQ=255)

                                                  gACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtggctggc                                       >  1:4659443/1‑93 (MQ=255)

                                                  gACAAAACCTCAGCCTACTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGtggctggc                                       >  1:688785/1‑93 (MQ=255)

                                                     aaaaCCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgc                                    <  2:3466117/93‑1 (MQ=255)

                                                      aaaCCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgct                                   >  1:5279200/1‑93 (MQ=255)

                                                       aaCCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTTCGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgctg                                  >  1:566262/1‑93 (MQ=255)

                                                       aaCCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTTGCTGgctgctg                                  >  2:4621701/1‑93 (MQ=255)

                                                       aaCCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgctg                                  >  1:4143239/1‑93 (MQ=255)

                                                       aaCCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgctg                                  >  2:3736660/1‑93 (MQ=255)

                                                         ccTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGgctgctgct                                <  2:594944/93‑1 (MQ=255)

                                                          cTCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTTTCGCAATCGTGGCTGGCTGCTGCTa                               >  1:3451355/1‑93 (MQ=255)

                                                           tCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTAc                              >  1:2707603/1‑93 (MQ=255)

                                                           tCAGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTAc                              >  1:1364689/1‑93 (MQ=255)

                                                             aGCCTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTAccc                            >  1:2403292/1‑93 (MQ=255)

                                                               ccTGCTGAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCAt                          <  1:773010/93‑1 (MQ=255)

                                                                ctgctgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCAtt                         <  2:5102882/93‑1 (MQ=255)

                                                                ctgctgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCAtt                         >  2:3887957/1‑93 (MQ=255)

                                                                 tgctgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTg                        >  1:3236141/1‑93 (MQ=255)

                                                                 tgctgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTg                        <  1:3430352/93‑1 (MQ=255)

                                                                 tgctgAACGTTGGTTCATTGGTCAGCGTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTg                        <  2:6083126/93‑1 (MQ=255)

                                                                    tgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCt                     >  1:3064378/1‑93 (MQ=255)

                                                                    tgAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCt                     >  2:2875620/1‑93 (MQ=255)

                                                                     gAACGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTa                    <  1:5822653/93‑1 (MQ=255)

                                                                      aaCGTTGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTAt                   <  1:2448633/93‑1 (MQ=255)

                                                                          ttGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCt               <  1:4462937/93‑1 (MQ=255)

                                                                          ttGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCt               <  2:3663736/93‑1 (MQ=255)

                                                                          ttGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCt               <  2:2947961/93‑1 (MQ=255)

                                                                          ttGGTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCt               >  1:5465973/1‑93 (MQ=255)

                                                                            ggTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTCCTCCTACCCATTGTGCATGCCttt             >  1:1914442/1‑93 (MQ=255)

                                                                             gTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTg            <  2:2017019/93‑1 (MQ=255)

                                                                             gTTCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTg            >  1:2117944/1‑93 (MQ=255)

                                                                              ttCATTGGTCAGCTTGATGATGTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGCTGCTTGCTGCTCCTACCCATTGTCTATGCCTTTgc           >  1:4242646/1‑93 (MQ=255)

                                                                               tCATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTgcg          <  2:3019255/93‑1 (MQ=255)

                                                                                cATTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTgcgc         <  2:3302642/93‑1 (MQ=255)

                                                                                 aTTGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCt        <  2:3132090/93‑1 (MQ=255)

                                                                                  ttGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCtt       >  2:5011887/1‑93 (MQ=255)

                                                                                  ttGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCtt       <  2:2388100/93‑1 (MQ=255)

                                                                                   tGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTa      <  1:281505/93‑1 (MQ=255)

                                                                                   tGGTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTa      <  1:2996353/93‑1 (MQ=255)

                                                                                    tgTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTAt     <  1:5906828/92‑1 (MQ=255)

                                                                                    ggTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTTGCTGCTGCTACCCATTGTCTATGCCTTTTCGGTTAt     >  2:5684693/1‑93 (MQ=255)

                                                                                     gTCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATc    >  2:5702500/1‑93 (MQ=255)

                                                                                      tCAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGGCTTTGCGCTTATCa   >  1:5098642/1‑93 (MQ=255)

                                                                                      tCAGCTTGATGATCTGTACGGGAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCa   >  2:2285684/1‑93 (MQ=255)

                                                                                       cAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCCTTGTCTATGCCTTTGCGCTTATCaa  >  2:3400151/1‑93 (MQ=255)

                                                                                       cAGCTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCaa  <  1:1338104/93‑1 (MQ=255)

CTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAACCTCAGCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAA  >  NC_000913/2746110‑2746289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`