BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,754,691	T→C	V171A (GTT→GCT)	intA →	prophage CP4‑57 integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,754,691	0	T	C	100.0%	964.3 / NA	293	V171A (GTT→GCT)	intA	prophage CP4‑57 integrase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (138/155); total (138/155)

Only 100 of 294 total aligned reads displayed.

CAATCGGTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGTTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAA  >  NC_000913/2754601‑2754780

cAATCGGTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTc                                                                                         <  2:243118/93‑1 (MQ=255)

cAATCGGTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTc                                                                                         <  2:6074295/93‑1 (MQ=255)

 aaTCGGTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTcg                                                                                        >  1:478963/1‑93 (MQ=255)

  aTCGGTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTcgc                                                                                       <  2:3707933/93‑1 (MQ=255)

  aTCGGTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTcgc                                                                                       >  2:4387266/1‑93 (MQ=255)

      gTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCgccgcc                                                                                   <  2:3401925/93‑1 (MQ=255)

       tGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCt                                                                                  <  1:6047434/93‑1 (MQ=255)

       tGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCt                                                                                  >  1:2767240/1‑93 (MQ=255)

       ggATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCt                                                                                  <  2:6424333/92‑1 (MQ=255)

        gATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCtt                                                                                 <  1:952135/93‑1 (MQ=255)

        gATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCtt                                                                                 <  1:1418650/93‑1 (MQ=255)

         atatCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCttt                                                                                <  1:4689254/93‑1 (MQ=255)

          tatCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTg                                                                               >  2:6186875/1‑93 (MQ=255)

            tCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTc                                                                             >  2:4601588/1‑93 (MQ=255)

             cAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCa                                                                            >  2:2628901/1‑93 (MQ=255)

             cAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCa                                                                            >  2:4481402/1‑93 (MQ=255)

             cAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCa                                                                            <  2:1098270/93‑1 (MQ=255)

              aGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAg                                                                           <  2:5242303/93‑1 (MQ=255)

              aGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAg                                                                           >  1:3073387/1‑93 (MQ=255)

               gTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGc                                                                          <  2:2262413/93‑1 (MQ=255)

               gTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGc                                                                          <  2:6255118/93‑1 (MQ=255)

                tATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCg                                                                         >  2:4165923/1‑93 (MQ=255)

                  tCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTa                                                                       <  2:6504839/93‑1 (MQ=255)

                     cTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTa                                                                    >  2:3080389/1‑93 (MQ=255)

                      tGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTaa                                                                   >  1:74598/1‑93 (MQ=255)

                      tGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTaa                                                                   <  2:1980350/93‑1 (MQ=255)

                      tGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTaa                                                                   <  1:2950272/93‑1 (MQ=255)

                        agaTTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACg                                                                 <  1:3896134/93‑1 (MQ=255)

                         gaTTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGa                                                                >  1:3647374/1‑93 (MQ=255)

                         gaTTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGa                                                                >  2:4973192/1‑93 (MQ=255)

                          aTTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGaa                                                               <  1:4639892/93‑1 (MQ=255)

                          aTTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGaa                                                               <  1:2528212/93‑1 (MQ=255)

                            tAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGt                                                             <  2:4680763/93‑1 (MQ=255)

                            tAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGt                                                             <  1:1951214/93‑1 (MQ=255)

                              aGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCa                                                           <  2:782007/93‑1 (MQ=255)

                               ggCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCAt                                                          >  2:5412838/1‑93 (MQ=255)

                               ggCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCAt                                                          >  2:6637861/1‑93 (MQ=255)

                               ggCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCAt                                                          >  2:3517976/1‑93 (MQ=255)

                               ggCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCAt                                                          >  1:3135882/1‑93 (MQ=255)

                                gCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATg                                                         >  1:5517594/1‑93 (MQ=255)

                                gCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATg                                                         >  2:2746772/1‑93 (MQ=255)

                                gCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATg                                                         <  2:1011698/93‑1 (MQ=255)

                                 cTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGa                                                        >  1:5902889/1‑93 (MQ=255)

                                  tCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGAt                                                       <  2:4711564/93‑1 (MQ=255)

                                      aCTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTAt                                                   >  1:4857709/1‑93 (MQ=255)

                                       ctctGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATg                                                  >  2:2021272/1‑93 (MQ=255)

                                        tctGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATgc                                                 >  2:4053385/1‑93 (MQ=255)

                                        tctGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATgc                                                 <  1:2250852/93‑1 (MQ=255)

                                          tGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATgcgc                                               <  2:5571145/93‑1 (MQ=255)

                                             ttAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGa                                            <  2:5159728/93‑1 (MQ=255)

                                              tAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGaa                                           >  1:6672197/1‑93 (MQ=255)

                                              tAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGaa                                           >  1:4411931/1‑93 (MQ=255)

                                               aaaGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAac                                          >  2:1500245/1‑93 (MQ=255)

                                                 aGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAacac                                        >  1:5222357/1‑93 (MQ=255)

                                                 aGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAacaa                                        >  1:1270637/1‑92 (MQ=255)

                                                  gCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAAcaca                                       <  2:31656/93‑1 (MQ=255)

                                                  gCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAAcaca                                       <  1:5206507/93‑1 (MQ=255)

                                                   cAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAg                                      <  1:4023726/93‑1 (MQ=255)

                                                   cAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAg                                      <  1:2809722/93‑1 (MQ=255)

                                                     gTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGc                                    <  1:2102837/93‑1 (MQ=255)

                                                     gTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGc                                    >  2:2560313/1‑93 (MQ=255)

                                                     gTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGc                                    >  1:569826/1‑93 (MQ=255)

                                                     gTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGc                                    >  1:4273376/1‑93 (MQ=255)

                                                      ttCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGcc                                   <  2:2339810/93‑1 (MQ=255)

                                                         aGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAGCGAAGTCATGATTTATGCGCAGAACACAGGCCtga                                >  2:5943170/1‑93 (MQ=255)

                                                          gCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgat                               <  1:1800003/93‑1 (MQ=255)

                                                          gCCGGTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgat                               <  1:2650300/93‑1 (MQ=255)

                                                             gtttCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgattga                            <  1:579/91‑1 (MQ=255)

                                                             ggTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgattga                            <  2:520474/93‑1 (MQ=255)

                                                             ggTTCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCtgattga                            <  1:6413487/93‑1 (MQ=255)

                                                               ttCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATg                          >  1:804809/1‑93 (MQ=255)

                                                                tCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGc                         >  1:1368157/1‑93 (MQ=255)

                                                                tCAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGc                         <  1:2458334/93‑1 (MQ=255)

                                                                 cAGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCt                        >  2:1816068/1‑93 (MQ=255)

                                                                  aGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTg                       <  2:3862430/93‑1 (MQ=255)

                                                                  aGGCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTg                       >  1:1082477/1‑93 (MQ=255)

                                                                   ggCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGt                      <  1:2867019/93‑1 (MQ=255)

                                                                    gCCAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGtt                     <  1:5429962/93‑1 (MQ=255)

                                                                      cAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTcc                   <  1:1197015/93‑1 (MQ=255)

                                                                      cAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTcc                   <  2:3998920/93‑1 (MQ=255)

                                                                      cAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTcc                   <  1:6216987/93‑1 (MQ=255)

                                                                      cAGAGGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTcc                   >  2:2135462/1‑93 (MQ=255)

                                                                       agagGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCt                  >  1:1848392/1‑93 (MQ=255)

                                                                       agagGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCt                  >  2:2971715/1‑93 (MQ=255)

                                                                        gagGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTa                 >  1:6283181/1‑93 (MQ=255)

                                                                         agGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAg                <  2:4561358/93‑1 (MQ=255)

                                                                         agGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAg                <  2:1749883/93‑1 (MQ=255)

                                                                         agGTGCATTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAg                <  2:472804/93‑1 (MQ=255)

                                                                          ggTGCATTAGAGACTGCTCGCAGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAgt               >  2:6703038/1‑93 (MQ=255)

                                                                               aTTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTaaa          <  1:1092166/93‑1 (MQ=255)

                                                                               aTTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTaaa          >  2:579784/1‑93 (MQ=255)

                                                                               aTTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTaaa          <  2:1550395/93‑1 (MQ=255)

                                                                               aTTAGAGACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTaaa          <  2:138734/93‑1 (MQ=255)

                                                                                  agagACTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGCTGCTGTTTCTAGTGTAAatat       >  2:3647167/1‑93 (MQ=255)

                                                                                   gagaCTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATc      <  1:2182736/93‑1 (MQ=255)

                                                                                     gaCTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCgg    >  2:1208243/1‑93 (MQ=255)

                                                                                     gaCTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCgg    <  1:4540793/93‑1 (MQ=255)

                                                                                      aCTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGa   <  2:2845877/93‑1 (MQ=255)

                                                                                      aCTGCTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGa   >  1:378732/1‑93 (MQ=255)

                                                                                       cTGCTCGCCGCCTTTGTCAGAGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGaa  >  1:3366807/1‑93 (MQ=255)

CAATCGGTGATATCAGTATCACTGAGATTAAGGCTCATACTCTGGTTAAAGCAGTTCAGCCGGTTCAGGCCAGAGGTGCATTAGAGACTGTTCGCCGCCTTTGTCAGCGTATTAACGAAGTCATGATTTATGCGCAGAACACAGGCCTGATTGATGCTGTTCCTAGTGTAAATATCGGAA  >  NC_000913/2754601‑2754780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`