BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,774,233	T→C	G98G (GGT→GGC)	yfjX →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,774,233	0	T	C	99.6%	704.2 / ‑4.8	221	G98G (GGT→GGC)	yfjX	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (120/100); total (120/101)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

Only 100 of 235 total aligned reads displayed.

TTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGAC  >  NC_000913/2774143‑2774322

ttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGgcgc                                                                                         >  1:519298/1‑93 (MQ=255)

ttACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGgcgc                                                                                         <  2:6377395/93‑1 (MQ=255)

   cacGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGa                                                                                      <  2:130378/93‑1 (MQ=255)

     cGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGaaa                                                                                    <  1:863039/93‑1 (MQ=255)

      gCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAAt                                                                                   <  2:1259032/93‑1 (MQ=255)

      gCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAAt                                                                                   <  1:4935746/93‑1 (MQ=255)

        tCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGa                                                                                 <  2:4521485/93‑1 (MQ=255)

         cAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAg                                                                                <  1:911588/93‑1 (MQ=255)

          aGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAgc                                                                               <  1:995898/93‑1 (MQ=255)

          aGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAgc                                                                               <  1:1708648/93‑1 (MQ=255)

          aGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGGGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATTAgc                                                                               >  2:1081773/1‑93 (MQ=255)

           gCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAgcg                                                                              >  2:3724953/1‑93 (MQ=255)

           gCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATAGCGCGGAAATGAgcg                                                                              >  1:703232/1‑93 (MQ=255)

               tGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGa                                                                          >  2:5026686/1‑93 (MQ=255)

               tGGCGGAGCGTTTATGGCTCCTGATGCTGACGGGGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGa                                                                          >  1:6282779/1‑93 (MQ=255)

                ggCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGaa                                                                         >  2:2586185/1‑93 (MQ=255)

                 gCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAg                                                                        <  1:1931562/93‑1 (MQ=255)

                  cGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGc                                                                       <  1:5890756/93‑1 (MQ=255)

                  cGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGc                                                                       <  2:3587009/93‑1 (MQ=255)

                   ggAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGcc                                                                      >  2:2686775/1‑93 (MQ=255)

                   ggAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGcc                                                                      <  2:826931/93‑1 (MQ=255)

                    gAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATCGCGCGGAAATGAGCGCGGAAGCCg                                                                     >  1:544855/1‑93 (MQ=255)

                      gCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCa                                                                   >  1:1870221/1‑93 (MQ=255)

                      gCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCa                                                                   >  1:2942079/1‑93 (MQ=255)

                         tttATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGt                                                                >  2:2738562/1‑93 (MQ=255)

                         tttATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGt                                                                <  2:5706277/93‑1 (MQ=255)

                         tttATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGt                                                                <  1:584275/93‑1 (MQ=255)

                            aTGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATc                                                             <  1:2440422/93‑1 (MQ=255)

                            aTGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGGATc                                                             >  1:3600321/1‑93 (MQ=255)

                              ggCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGc                                                           <  2:4031694/93‑1 (MQ=255)

                                cTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTg                                                         <  1:2618554/93‑1 (MQ=255)

                                cTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTg                                                         >  2:6175693/1‑93 (MQ=255)

                                cTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTg                                                         <  2:2001388/93‑1 (MQ=255)

                                 tCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGt                                                        >  2:4212089/1‑93 (MQ=255)

                                  ccTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTc                                                       <  1:275220/93‑1 (MQ=255)

                                  ccTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTc                                                       <  1:3777217/93‑1 (MQ=255)

                                  ccTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTc                                                       <  1:144781/93‑1 (MQ=255)

                                  ccTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAAGGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTc                                                       <  1:3312087/93‑1 (MQ=255)

                                    tGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTg                                                     <  2:6626434/93‑1 (MQ=255)

                                      aTGCTGACGGTGACGATAAATGGCATTGGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGcc                                                   <  2:3644806/93‑1 (MQ=255)

                                       tGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCt                                                  >  2:2198109/1‑93 (MQ=255)

                                       tGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCt                                                  <  1:2873618/93‑1 (MQ=255)

                                       tGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCt                                                  >  2:476797/1‑93 (MQ=255)

                                         cTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGa                                                <  1:5061013/93‑1 (MQ=255)

                                         cTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGa                                                <  2:346224/93‑1 (MQ=255)

                                          tGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGAt                                               >  1:4798204/1‑93 (MQ=255)

                                          tGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGAt                                               >  1:4147812/1‑93 (MQ=255)

                                          tGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGAt                                               <  2:721061/93‑1 (MQ=255)

                                           gACGGTGACGATAAATGGCATTTGCTCAACGTCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGAtt                                              <  2:1334701/93‑1 (MQ=255)

                                            aCGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTg                                             <  1:1502442/93‑1 (MQ=255)

                                              ggTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGaa                                           <  1:3516610/93‑1 (MQ=255)

                                              ggTGACGATAAATGGCATTGCCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGaa                                           <  1:813152/93‑1 (MQ=255)

                                               gTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAat                                          <  2:3056513/93‑1 (MQ=255)

                                                tGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAata                                         >  1:6515551/1‑93 (MQ=255)

                                                 gACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAatat                                        <  1:1055873/93‑1 (MQ=255)

                                                  aCGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAAtata                                       <  1:2386858/93‑1 (MQ=255)

                                                   cGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAg                                      >  2:2327518/1‑93 (MQ=255)

                                                   cGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAg                                      >  1:3417115/1‑93 (MQ=255)

                                                      tAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCa                                   >  1:2571066/1‑93 (MQ=255)

                                                      tAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCa                                   >  1:607259/1‑93 (MQ=255)

                                                      tAAATGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCa                                   >  2:1705881/1‑93 (MQ=255)

                                                       aaaTGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCAt                                  <  1:1567756/93‑1 (MQ=255)

                                                       aaaTGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCAt                                  >  1:4264245/1‑93 (MQ=255)

                                                       aaaTGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCAt                                  <  1:5497744/93‑1 (MQ=255)

                                                        aagggCATTTGCTCAACGCCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc                                 <  1:2697360/90‑1 (MQ=255)

                                                        aaTGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc                                 >  1:5205866/1‑93 (MQ=255)

                                                        aaTGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc                                 >  2:5196486/1‑93 (MQ=255)

                                                        aaTGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc                                 >  1:6484183/1‑93 (MQ=255)

                                                        aaTGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATc                                 <  2:4396480/93‑1 (MQ=255)

                                                         aTGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCa                                <  1:75853/93‑1 (MQ=255)

                                                         aTGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCa                                >  2:2778506/1‑93 (MQ=255)

                                                          tGGCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCAc                               <  1:1003613/93‑1 (MQ=255)

                                                            gCATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgc                             >  2:240440/1‑93 (MQ=255)

                                                             cATTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcc                            >  1:5084340/1‑93 (MQ=255)

                                                              aTTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct                           <  2:329322/93‑1 (MQ=255)

                                                              aTTTGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcct                           >  1:222494/1‑93 (MQ=255)

                                                               tttGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcctg                          >  2:504257/1‑93 (MQ=255)

                                                                ttGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcctgc                         <  2:2109558/93‑1 (MQ=255)

                                                                ttGCTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcctgc                         >  1:2855687/1‑93 (MQ=255)

                                                                  gCTCATCGGCATGAATGGCAATGCCGCGAAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACgcctgcct                       <  1:6161888/93‑1 (MQ=37)

                                                                   cTCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTc                      <  2:4911007/93‑1 (MQ=255)

                                                                    tCAACGGCATGAGTGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCa                     <  2:2264231/93‑1 (MQ=255)

                                                                    tCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCa                     >  2:5122113/1‑93 (MQ=255)

                                                                    tCAACGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCa                     >  2:4683344/1‑93 (MQ=255)

                                                                       aCGGCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCg                  >  1:5636056/1‑93 (MQ=255)

                                                                          gCATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAAt               <  1:4465734/93‑1 (MQ=255)

                                                                          gCATGAATGGCAATGGCGCGAAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAAt               <  1:5997264/93‑1 (MQ=255)

                                                                           cATGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATg              >  1:5778364/1‑93 (MQ=255)

                                                                            aTGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGc             >  1:556202/1‑93 (MQ=255)

                                                                            aTGAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGc             >  2:14962/1‑93 (MQ=255)

                                                                              gAATGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGa           >  2:296589/1‑93 (MQ=255)

                                                                               aatggcaatggcGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGAt          <  1:4118152/93‑1 (MQ=255)

                                                                                 tGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGc        >  1:4351817/1‑93 (MQ=255)

                                                                                 tGGCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGc        >  1:1309777/1‑93 (MQ=255)

                                                                                  ggCAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGcc       <  1:3245253/93‑1 (MQ=255)

                                                                                    cAATGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCAGCACGCCTGCCTCACCGAATGCGATGCCAt     >  1:6595771/1‑93 (MQ=255)

                                                                                     aaTGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATg    >  1:3005338/1‑93 (MQ=255)

                                                                                     aaTGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATg    >  2:2355049/1‑93 (MQ=255)

                                                                                      aTGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGa   >  1:4887676/1‑93 (MQ=255)

                                                                                      aTGGCGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGa   >  1:2111222/1‑93 (MQ=255)

TTACACGCTCAGCAATGGCGGAGCGTTTATGGCTCCTGATGCTGACGGTGACGATAAATGGCATTTGCTCAACGGCATGAATGGCAATGGTGCGGAAATGAGCGCGGAAGCCGCAGGTATCGCTGTCTGCCTGATTGAATATAGCCATCACGCCTGCCTCACCGAATGCGATGCCATGAC  >  NC_000913/2774143‑2774322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`