BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,844,596	A→G	R280R (CGT→CGC)	hycD ←	membrane‑spanning protein of hydrogenase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,844,596	0	A	G	100.0%	793.6 / NA	244	R280R (CGT→CGC)	hycD	membrane‑spanning protein of hydrogenase 3
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (152/92); total (152/92)

Only 100 of 251 total aligned reads displayed.

TTTAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACC  >  NC_000913/2844507‑2844685

tttAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCgg                                                                                        <  1:4985068/93‑1 (MQ=255)

tttAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCgg                                                                                        <  1:4060882/93‑1 (MQ=255)

tttAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCgg                                                                                        <  1:1924315/93‑1 (MQ=255)

 ttAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCggg                                                                                       >  1:1218938/1‑93 (MQ=255)

  tAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGc                                                                                      >  1:1241877/1‑93 (MQ=255)

    aTCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCa                                                                                    >  1:5474905/1‑93 (MQ=255)

     tCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCAt                                                                                   >  2:3401822/1‑93 (MQ=255)

      cACGCCGCGAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATg                                                                                  <  2:534101/93‑1 (MQ=255)

       aCGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGc                                                                                 >  2:1212164/1‑93 (MQ=255)

          ccgccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGt                                                                              >  1:775878/1‑93 (MQ=255)

          ccgccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGt                                                                              <  1:3767434/93‑1 (MQ=255)

          ccgccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGt                                                                              <  2:4214821/93‑1 (MQ=255)

           cgccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGGAATATCAAGACGCAGGCGGGCCCTGCTGtt                                                                             >  1:1262853/1‑93 (MQ=255)

             ccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGtttt                                                                           <  2:2427080/93‑1 (MQ=255)

             ccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGtttt                                                                           >  1:1202788/1‑93 (MQ=255)

              cAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTc                                                                          <  1:937274/93‑1 (MQ=255)

              cAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTc                                                                          >  2:839799/1‑93 (MQ=255)

                gCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGa                                                                        >  1:6667777/1‑93 (MQ=255)

                gCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGa                                                                        >  1:5905496/1‑93 (MQ=255)

                  aaGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAAc                                                                      >  1:3115586/1‑93 (MQ=255)

                  aaGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAAc                                                                      <  2:3923187/93‑1 (MQ=255)

                   aGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACa                                                                     >  2:5862042/1‑93 (MQ=255)

                   aGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACa                                                                     >  1:4416164/1‑93 (MQ=255)

                   aGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACa                                                                     >  2:4583808/1‑93 (MQ=255)

                   aGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACa                                                                     <  2:5782313/93‑1 (MQ=255)

                    ggAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAg                                                                    >  2:2565458/1‑93 (MQ=255)

                     gagaCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAgc                                                                   >  2:887499/1‑93 (MQ=255)

                     gagaCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAgc                                                                   >  2:3170797/1‑93 (MQ=255)

                      agaCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAgcg                                                                  >  2:3640977/1‑93 (MQ=255)

                       gaCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAgcgc                                                                 >  2:2064466/1‑93 (MQ=255)

                        aCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGcgcg                                                                >  1:821287/1‑93 (MQ=255)

                          gAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGAt                                                              >  1:1035061/1‑93 (MQ=255)

                          gAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGAt                                                              >  2:4960346/1‑93 (MQ=255)

                           aaCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATa                                                             <  2:3777500/93‑1 (MQ=255)

                           aaCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATa                                                             >  1:2111076/1‑93 (MQ=255)

                           aaCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATa                                                             >  1:4552630/1‑93 (MQ=255)

                           aaCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATa                                                             >  1:1821239/1‑93 (MQ=255)

                            aCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATaa                                                            >  1:1021468/1‑93 (MQ=255)

                            aCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATaa                                                            >  1:3698248/1‑93 (MQ=255)

                             cGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAAc                                                           <  1:5091393/93‑1 (MQ=255)

                              gCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAAcc                                                          >  1:6366243/1‑93 (MQ=255)

                              acTAAAAATTCAAAGCCAAACCCAGCCCAAGTAATGGGCGGAATAATAACAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAAcc                                                          <  1:2987303/92‑1 (MQ=255)

                               cTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCa                                                         >  1:2856666/1‑93 (MQ=255)

                                  aaaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGa                                                      <  2:1906958/93‑1 (MQ=255)

                                     aTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGc                                                   >  2:2209381/1‑93 (MQ=255)

                                     aTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGc                                                   <  2:2013507/93‑1 (MQ=255)

                                      tGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGcc                                                  <  2:3111246/93‑1 (MQ=255)

                                      tGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGcc                                                  <  1:1142157/93‑1 (MQ=255)

                                          aaGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGacc                                              >  2:3852344/1‑93 (MQ=255)

                                            gCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGaccac                                            >  1:3998843/1‑93 (MQ=255)

                                            gCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGaccac                                            >  1:338336/1‑93 (MQ=255)

                                            gCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGACGaccac                                            >  1:27928/1‑93 (MQ=255)

                                             ccAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGaccacc                                           <  2:2793963/93‑1 (MQ=255)

                                             ccAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGaccacc                                           >  2:93320/1‑93 (MQ=255)

                                               aaaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAg                                         <  1:2724766/93‑1 (MQ=255)

                                               aaaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAg                                         >  1:1960311/1‑93 (MQ=255)

                                                aaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGt                                        <  2:622107/93‑1 (MQ=255)

                                                aaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGt                                        <  2:4492738/93‑1 (MQ=255)

                                                aaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGt                                        >  2:4076257/1‑93 (MQ=255)

                                                 aCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGtt                                       >  2:2308344/1‑93 (MQ=255)

                                                 aCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGtt                                       <  2:465720/93‑1 (MQ=255)

                                                 aCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGtt                                       >  2:2945258/1‑93 (MQ=255)

                                                   ccagcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGtttt                                     <  1:1630413/93‑1 (MQ=255)

                                                    cagcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTa                                    <  2:6299429/93‑1 (MQ=255)

                                                      gcccagGTAATGCGCGGAGCAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACg                                  >  1:3279760/1‑93 (MQ=255)

                                                        ccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGAt                                >  2:4018706/1‑93 (MQ=255)

                                                        ccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGAt                                >  2:5130045/1‑93 (MQ=255)

                                                         cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATg                               <  1:5369670/93‑1 (MQ=255)

                                                         cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATg                               >  2:6487334/1‑93 (MQ=255)

                                                          agGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATgg                              >  2:6579117/1‑93 (MQ=255)

                                                           ggTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGc                             >  1:4253479/1‑93 (MQ=255)

                                                            gTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCa                            >  2:4673160/1‑93 (MQ=255)

                                                               aTGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATc                         >  1:5709508/1‑93 (MQ=255)

                                                                tGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCa                        >  2:2872660/1‑93 (MQ=255)

                                                                  cgcgGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCAcc                      <  2:4327073/93‑1 (MQ=255)

                                                                   gcgGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCa                     <  1:3533078/93‑1 (MQ=255)

                                                                   gcgGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCa                     <  2:1128839/93‑1 (MQ=255)

                                                                    cgGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAg                    <  1:6428744/93‑1 (MQ=255)

                                                                     ggAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgc                   <  1:6157430/93‑1 (MQ=255)

                                                                     ggAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgc                   >  1:2561174/1‑93 (MQ=255)

                                                                      gAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgcg                  >  2:4376693/1‑93 (MQ=255)

                                                                      gAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgcg                  >  2:294157/1‑93 (MQ=255)

                                                                        gTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGcc                >  2:3343139/1‑93 (MQ=255)

                                                                         tAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCca               <  2:5906863/93‑1 (MQ=255)

                                                                          aaTATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcag              >  2:5454348/1‑93 (MQ=255)

                                                                           atatCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagc             >  2:6172701/1‑93 (MQ=255)

                                                                           atatCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagc             >  2:1372461/1‑93 (MQ=255)

                                                                            tatCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagca            >  1:2445224/1‑93 (MQ=255)

                                                                            tatCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagca            >  2:5993968/1‑93 (MQ=255)

                                                                            tatCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGCTTTACGATGGCAATCACCAGCGCcagca            >  2:5572732/1‑93 (MQ=255)

                                                                              tCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcagc          <  2:2074687/93‑1 (MQ=255)

                                                                              tCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcagc          >  1:988307/1‑93 (MQ=255)

                                                                                  gACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTc      >  1:1395735/1‑93 (MQ=255)

                                                                                  gACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTc      >  1:3398078/1‑93 (MQ=255)

                                                                                   aCGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTcc     <  1:2299639/93‑1 (MQ=255)

                                                                                    cGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCa    >  2:1633105/1‑93 (MQ=255)

                                                                                     gCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCAc   >  2:5079755/1‑93 (MQ=255)

                                                                                     gCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCAc   <  2:1849725/93‑1 (MQ=255)

                                                                                      cAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCAcc  <  1:5323647/93‑1 (MQ=255)

                                                                                      cAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCAcc  <  1:687749/93‑1 (MQ=255)

TTTAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACC  >  NC_000913/2844507‑2844685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`